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Full-Text Articles in Aquaculture and Fisheries

Diversity Of The Ohio River Bacterial Communities Using Next-Generation Sequencing Techniques, Emily Michele Anneken Jan 2012

Diversity Of The Ohio River Bacterial Communities Using Next-Generation Sequencing Techniques, Emily Michele Anneken

Theses, Dissertations and Capstones

The ability to sequence bacterial genetic material directly from environmental samples has unlocked the field of metagenomics. Next-generation sequencing techniques, such as pyrosequencing, have been instrumental in providing knowledge about bacterial communities. This study focused on identifying the bacterial diversity of the Ohio River via pyrosequencing and compared the diversity of cultivable bacteria versus the entire river community. To ensure the maximum number of cultivable bacteria was obtained, cultures were grown on nonselective media and incubated at river temperature. Bacterial DNA was extracted, sequenced, and classified. The dominant phyla for the Ohio River included Cyanobacteria (38-66% of the total), Actinobacteria …


The Effect Of Auditory Call Playback On Anuran Detection And Capture Rates, Derek Adam Bozzell Jan 2012

The Effect Of Auditory Call Playback On Anuran Detection And Capture Rates, Derek Adam Bozzell

Theses, Dissertations and Capstones

Calls of male anurans during breeding seasons are species-specific identification tools. However, males cease calling after any nearby disturbance, including those of researchers. I proposed a variation on current methods that attempts to reduce this lag in calling after researcher-created disturbance by utilizing the propensity for competition in male frogs. I surveyed 14 breeding sites in Cabell and Wayne counties during the 2010 and 2011 breeding seasons. First, I used traditional visual encounter surveys (VESs). After using automated recording devices to gather site-specific recordings of calls of all species present, I conducted secondary VESs while playing these playlists over a …


A Reproductive And Trophic Transfer Study Associated With Selenium Concentrations In The Upper Mud River Watershed, Amanda Lee Wilson Jan 2012

A Reproductive And Trophic Transfer Study Associated With Selenium Concentrations In The Upper Mud River Watershed, Amanda Lee Wilson

Theses, Dissertations and Capstones

Selenium in mining-related discharges has created concern in the Appalachian Region where coal is a significant resource. In West Virginia, evaluation of streams receiving mining discharges focused attention on the Mud River watershed where bioaccumulation of selenium was highest in preliminary surveys. Chronic exposure (mainly dietary) of mature female fish to selenium has the potential to cause developmental abnormalities in developing embryos due to the maternal transfer of selenium into the eggs. Literature suggests that factors affecting the bioaccumulation rate of selenium, and the concentration of selenium associated with the aforementioned effects are site-specific. The purpose of this study was …


Freshwater Mussel Populations Of The Monongahela River, Pa And Evaluation Of The Orsanco Copper Pole Substrate Sampling Technique Using G.I.S. Interpolation With Geometric Means, Jonathan Hart Jan 2012

Freshwater Mussel Populations Of The Monongahela River, Pa And Evaluation Of The Orsanco Copper Pole Substrate Sampling Technique Using G.I.S. Interpolation With Geometric Means, Jonathan Hart

Theses, Dissertations and Capstones

Large river studies for freshwater mussel populations and habitat in the Monongahela River within Pennsylvania have been almost non-existent over the past century. Aquatic diversity and water quality have been impaired in the Monongahela River since the Industrial Revolution and early impoundments were constructed to control the river. To date, there have been no thorough mussel population studies conducted on the Pennsylvanian Monongahela River proper since A.E. Ortmann in 1919. The mussel population accounts for this large river system are invaluable accounts of the aquatic condition of the Monongahela River. Mussel populations and habitat within the river have diminished drastically …


The Status And Distribution Of Invasive Crayfishes And Their Effects On Native Crayfish Communities In West Virginia, Casey David Swecker Jan 2012

The Status And Distribution Of Invasive Crayfishes And Their Effects On Native Crayfish Communities In West Virginia, Casey David Swecker

Theses, Dissertations and Capstones

Introductions of non-native crayfish species have resulted in the global decline of native crayfish populations, including those in North America. The last large survey of crayfishes from West Virginia was in 1988 and 1989. In this thorough document Raymond Jezerinac, Whitney Stocker, and Donald Tarter identified three separate areas in West Virginia where non-native crayfish species have been introduced. One area is located in the Potomac River drainage in northeastern West Virginia commonly referred to as the eastern panhandle of the state. This is also the only drainage in West Virginia where the native spiny cheek crayfish (Orconectes limosus) occurs. …