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Uptake And Internal Distribution Of Exogenously Supplied Amino Acids In The Pacific Oyster, Crassostrea Gigas (Thunberg), Michael A. Rice, Grover C. Stephens
Uptake And Internal Distribution Of Exogenously Supplied Amino Acids In The Pacific Oyster, Crassostrea Gigas (Thunberg), Michael A. Rice, Grover C. Stephens
Michael A Rice
A technique is described in which irrigation of the mantle cavity of oysters, Crassostrea gigas, induces pumping at steady rates. Simultaneous net influx of 10 amino acids (asp, glu, asn, ser, gly, tau, ala, val, phe, lys) is observed by high-performance liquid chromatography (HPLC). Individual amino acids exhibit first-order exponential depletion from an initial medium concentration of 2.0 μM. Influx of radiolabeled alanine corresponds very closely to net entry of alanine as estimated by HPLC. Therefore, net entry of alanine from dilute solution (5 μM) occurs. The entry of alanine is well described by Michaelis-Menten kinetics with a maximum influx …