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Biology

2021

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Full-Text Articles in Aquaculture and Fisheries

Scorpionfish (Scorpaena Porcus) Neurocranium, Erin Porter, Cairone Reft, David Kerstetter Dec 2021

Scorpionfish (Scorpaena Porcus) Neurocranium, Erin Porter, Cairone Reft, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium preparation of a Scorpionfish (Scorpaena porcus) by Erin Porter and Cairone Reft for the Biology of Fishes course at Nova Southeastern University taught by Dr. Kerstetter.


A Global Agenda For Advancing Freshwater Biodiversity Research, Alain Maasri, Sonja C. Jähnig, Hendrik Freitag, 93 Co-Authors Dec 2021

A Global Agenda For Advancing Freshwater Biodiversity Research, Alain Maasri, Sonja C. Jähnig, Hendrik Freitag, 93 Co-Authors

Biology Faculty Publications

Global freshwater biodiversity is declining dramatically, and meeting the challenges of this crisis requires bold goals and the mobilisation of substantial resources. While the reasons are varied, investments in both research and conservation of freshwater biodiversity lag far behind those in the terrestrial and marine realms. Inspired by a global consultation, we identify 15 pressing priority needs, grouped into five research areas, in an effort to support informed stewardship of freshwater biodiversity. The proposed agenda aims to advance freshwater biodiversity research globally as a critical step in improving coordinated actions towards its sustainable management and conservation.


Genetic Effect Of Long-Term Angling Pressure On Bluegill Populations In Small Impoundments In Kansas, Morganne Borsh Nov 2021

Genetic Effect Of Long-Term Angling Pressure On Bluegill Populations In Small Impoundments In Kansas, Morganne Borsh

Three Minute Thesis (3MT®) at UNI

Bluegill (Lepomis macrochirus) is a versatile sport fish popularly fished across Kansas. Over the last decade, Bluegill catch rates have decreased, likely from high angler harvest rates of larger individuals. Large males that exhibit parental care have high growth rates and aggressively defend nests, making them vulnerable to angling. Males that display cuckoldry behavior reach maturity sooner, exhibit slower growth rates, and are generally smaller. The goal of this study is to determine if fisheries-induced evolution resulting from long-term harvest has led to a decline in the growth rate of Bluegill. A total of 100 individuals were sampled from ten …


24andmore: Using Genomics To Improve Walleye (Sander Vitreus) Stocking In Iowa, Rachel Mcdonnell Nov 2021

24andmore: Using Genomics To Improve Walleye (Sander Vitreus) Stocking In Iowa, Rachel Mcdonnell

Three Minute Thesis (3MT®) at UNI

Walleye, Sander vitreus, is one of the top gamefish in Iowa. Due to poor natural reproduction in many Iowa waterbodies, populations are managed with stocking. There are two strains of walleye used to stock Iowa’s inland waters: a river strain derived from the Mississippi River near Genoa, WI and a lake strain derived from Spirit Lake, IA. Each strain may have unique characteristics relative to their environment that could affect the fitness and success of fish stocked. The goal of this study is to understand the genetic diversity within and between each strain to offer stocking guidance to the Iowa …


Overfishing Drives Over One-Third Of All Sharks And Rays Toward A Global Extinction Crisis, Nicholas K. Dulvy, Nathan Pacoureau, Cassandra L. Rigby, Riley A. Pollom, Rima W. Jabado, David A. Ebert, Brittany Finucci, Caroline M. Pollock, Jessica Cheok, Danielle H. Derrick, Katelyn B. Herman, C. Samantha Sherman, Wade J. Vanderwright, Julia M. Lawson, Rachel H.L. Walls, John K. Carlson, Patricia Charvet, Kinattumkara K. Bineesh, Daniel Fernando, Gina M. Ralph, Jay H. Matsushiba, Craig Hilton-Taylor, Sonja V. Fordham, Colin A. Simpfendorfer Nov 2021

Overfishing Drives Over One-Third Of All Sharks And Rays Toward A Global Extinction Crisis, Nicholas K. Dulvy, Nathan Pacoureau, Cassandra L. Rigby, Riley A. Pollom, Rima W. Jabado, David A. Ebert, Brittany Finucci, Caroline M. Pollock, Jessica Cheok, Danielle H. Derrick, Katelyn B. Herman, C. Samantha Sherman, Wade J. Vanderwright, Julia M. Lawson, Rachel H.L. Walls, John K. Carlson, Patricia Charvet, Kinattumkara K. Bineesh, Daniel Fernando, Gina M. Ralph, Jay H. Matsushiba, Craig Hilton-Taylor, Sonja V. Fordham, Colin A. Simpfendorfer

Biological Sciences Faculty Publications

The scale and drivers of marine biodiversity loss are being revealed by the International Union for Conservation of Nature (IUCN) Red List assessment process. We present the first global reassessment of 1,199 species in Class Chondrichthyes-sharks, rays, and chimeras. The first global assessment (in 2014) concluded that one-quarter (24%) of species were threatened. Now, 391 (32.6%) species are threatened with extinction. When this percentage of threat is applied to Data Deficient species, more than one-third (37.5%) of chondrichthyans are estimated to be threatened, with much of this change resulting from new information. Three species are Critically Endangered (Possibly Extinct), representing …


Fisheries Science Update – October 2021: West Coast Demersal Scalefish Resource, Department Of Primary Industries And Regional Development, Western Australia Oct 2021

Fisheries Science Update – October 2021: West Coast Demersal Scalefish Resource, Department Of Primary Industries And Regional Development, Western Australia

Fisheries occasional publications

Key points: 2021 stock assessment outcomes • The West Coast Demersal Scalefish Resource (WCDSR) is halfway into a 20-year recovery plan following a period of overfishing in the 1990s and 2000s. • This latest assessment provides an important “health check” on the recovery status. • The recovery plan is based on maintaining recreational (including charter) and commercial sector’s total fishing mortality below catch limits and protecting key spawning aggregations to recover the WCDSR by 2030. • The Department of Primary Industries and Regional Development (DPIRD) tracks recovery progress by undertaking weight of evidence stock assessments of WCDSR indicator species dhufish …


Squid And Cuttlefish Resources Of Western Australia, Daniel E. Yeoh Phd, Danielle J. Johnston Phd, David C. Harris Oct 2021

Squid And Cuttlefish Resources Of Western Australia, Daniel E. Yeoh Phd, Danielle J. Johnston Phd, David C. Harris

Fisheries research reports

This report provides a description and assessment of the squid and cuttlefish resources of Western Australia, and all of the fishing activities (i.e. fisheries / fishing sectors) affecting these resources. Encompassed are multiple species in the orders Oegopsida and Myopsida (squid or ‘calamari’) and Sepiida (cuttlefish), including; southern calamari (Sepioteuthis australis), northern calamari (Sepioteuthis lessoniana), Gould’s squid (Nototodarus gouldi), loligo squids Uroteuthis (Photololigo) spp.,giant cuttlefish (Sepia apama), broadclub cuttlefish (Sepia latimanus) and pharaoh cuttlefish (Sepia pharaonis).


Ecological Risk Assessment For The Temperate Demersal Elasmobranch Resource, Department Of Primary Industries And Regional Development, Western Australia Oct 2021

Ecological Risk Assessment For The Temperate Demersal Elasmobranch Resource, Department Of Primary Industries And Regional Development, Western Australia

Fisheries research reports

No abstract provided.


2021 Assessment Of The Status Of The West Coast Demersal Scalefish Resource, David V. Fairclough, Sybrand Alex Hesp, Ainslie Denham, Emily A. Fisher, Rachel Marks, Karina L. Ryan, Elaine Lek, Rhys Allen, Brett M. Crisafulli Oct 2021

2021 Assessment Of The Status Of The West Coast Demersal Scalefish Resource, David V. Fairclough, Sybrand Alex Hesp, Ainslie Denham, Emily A. Fisher, Rachel Marks, Karina L. Ryan, Elaine Lek, Rhys Allen, Brett M. Crisafulli

Fisheries research reports

A recovery program for the West Coast Demersal Scalefish Resource was introduced between late 2007 and early 2010, based on the maintenance of retained catches of demersal species (overall suite and each indicator species) by both the commercial and recreational sectors below 50% of the catches reported in 2005/06 (original catch recovery benchmarks).

Catch reductions were aimed at reducing exploitation levels (F, long-term fishing mortality of the key indicator species’ stocks) to below the threshold reference point (F = M, the natural mortality rate), which would then allow stocks to recover to above the …


Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elaine Lek, Joshua Brown Sep 2021

Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elaine Lek, Joshua Brown

Fisheries research reports

Due to the species-specific nature of otoliths and given they are often the only part of the fish preserved when fish die, otolith catalogues can be used in numerous applications, such as diet studies in fish eating animals, including pinnipeds, fish and sea birds; archaeological purposes such as reconstructing indigenous people’s diets from otoliths found in middens or evolutionary history of fish species by comparing fossilized otoliths. Given the unique mixture of subtropical and temperate fish, including many endemic species that occur off the southwest corner of WA having a catalogue for this area is extremely important for people working …


Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elain Lek, Joshua Brown Sep 2021

Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elain Lek, Joshua Brown

Fisheries research reports

Due to the species-specific nature of otoliths and given they are often the only part of the fish preserved when fish die, otolith catalogues can be used in numerous applications, such as diet studies in fish eating animals, including pinnipeds, fish and sea birds; archaeological purposes such as reconstructing indigenous people’s diets from otoliths found in middens or evolutionary history of fish species by comparing fossilized otoliths. Given the unique mixture of subtropical and temperate fish, including many endemic species that occur off the southwest corner of WA having a catalogue for this area is extremely important for people working …


Squid And Cuttlefish Resources Of Western Australia, Daniel Yeoh, Danielle J. Johnston Phd, David C. Harris Sep 2021

Squid And Cuttlefish Resources Of Western Australia, Daniel Yeoh, Danielle J. Johnston Phd, David C. Harris

Fisheries research reports

No abstract provided.


Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elain Lek, Joshua Brown Sep 2021

Otoliths Of South-Western Australian Fish: A Photographic Catalogue, Chris Dowling, Kim Smith, Elain Lek, Joshua Brown

Fisheries research reports

No abstract provided.


Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold Aug 2021

Assessing The Short-Term Effects Of Translocation On Freshwater Mussels: Is Habitat Or Water Quality More Important?, Joshua D. Arnold

Masters Theses

Freshwater mussels (Order: Unionida) are very important to the function of aquatic ecosystems and are typically indicators of good water quality. They provide a valuable link between the water column and the benthic substrate in which they live and are a valuable food resource for many species of animals. However, most species native to North America are currently threatened with extinction, to the point that more than 70% of native freshwater mussels are listed as either threatened or endangered at the state or federal level. The cause of this decline can be attributed to historical over exploitation, habitat alteration, and …


Transcriptomics And Toxcast Data Identify Bioeffects In Zebrafish Embryos Exposed To Chemical Mixtures In An Effluent-Dominated Stream, Emma Meade Aug 2021

Transcriptomics And Toxcast Data Identify Bioeffects In Zebrafish Embryos Exposed To Chemical Mixtures In An Effluent-Dominated Stream, Emma Meade

Theses and Dissertations

Wastewater treatment plant (WWTP) effluent-dominated streams provide critical habitat for aquatic organisms but also continually expose them to complex mixtures of pharmaceuticals and other contaminants of emerging concern (CECs) that can potentially impair growth, behavior, and reproduction. Limited toxicity data on the adverse biological impacts of in vivo exposure to these mixtures make it difficult to assess risk for aquatic organisms, particularly with respect to pharmaceuticals whose designed bioactivity often extends beyond conventionally monitored biological pathways. To address this knowledge gap, the goal of this thesis was to identify biomarkers of exposure to complex CEC mixtures relevant to specific chemicals …


Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam Jul 2021

Effects Of Salinity On Eastern Oysters: Locating Lower-Salinity Tolerant Populations And Defining Resource Zones Suitable To Restoration, Fisheries, And Aquaculture., Lauren Swam

LSU Master's Theses

Eastern oysters (Crassostrea virginica) provide valuable ecosystem services and support a productive commercial industry in the northern Gulf of Mexico. Declining abundance from water quality changes and other factors drives development of management and restoration strategies focused on a comprehensive, metapopulation approach. Identifying oyster resource zones based on water quality combined with selective breeding of oysters adapted to specific conditions provides strategies to support aquaculture development and ensure resilient oyster populations and high production. Using 2015-2019 satellite-derived continuous salinity and temperature data for coastal Louisiana, this work created maps defining oyster resource zones supportive of (1) broodstock sanctuary …


Prawn Resource Of Exmouth Gulf Harvest Strategy : 2021-2026 : Version 2.0, Department Of Fisheries Jun 2021

Prawn Resource Of Exmouth Gulf Harvest Strategy : 2021-2026 : Version 2.0, Department Of Fisheries

Fisheries management papers

No abstract provided.


Marine Protected Species Identification Guide, Department Of Fisheries Jun 2021

Marine Protected Species Identification Guide, Department Of Fisheries

Fisheries occasional publications

No abstract provided.


Vignette 04: Olympia Oysters, Jodie Toft, Betsy Peabody May 2021

Vignette 04: Olympia Oysters, Jodie Toft, Betsy Peabody

Institute Publications

Olympia oysters (Ostrea lurida) are our only native oyster species here in the Salish Sea. Olympia oysters once covered an estimated 13-26% of the intertidal area in Puget Sound, mostly near the heads of inlets. A combination of overharvest, pollution, and habitat loss reduced the current population to less than 4% of historic numbers, though sparse numbers of Olympia oysters can still be found throughout most of their historic distribution. Looking to the future, as our region’s marine waters experience effects of climate change and ocean acidification (OA), native species such as the Olympia oyster may prove to …


Vignette 05: Blocking Culverts Impact Salmonid Survival, Northwest Indian Fisheries Commission May 2021

Vignette 05: Blocking Culverts Impact Salmonid Survival, Northwest Indian Fisheries Commission

Institute Publications

During the first six years of implementing the U.S. v. Washington culvert case injunction, the State of Washington has corrected 150 fish-blocking culverts in the Puget Sound Region. At the current rate, if additional support is not gained, the corrections of the remaining 799 culverts would be completed in 32 years or the year 2052.


Vignette 23: Indigenous Management Systems Can Promote More Sustainable Salmon Fisheries In The Salish Sea, William I. Atlas, Natalie C. Ban, Jonathan W. Moore, Adrian M. Tuohy, Spencer Greening, Andrea J. Reid, Nicole Morven, Elroy White, William G. Housty, Jess A. Housty, Christina N. Service, Larry Greba, Sam Harrison, Katherine Ir Butts, Elissa Sweeney-Bergen, Donna Macintyre, Matthew R. Sloat, Katrina Connors May 2021

Vignette 23: Indigenous Management Systems Can Promote More Sustainable Salmon Fisheries In The Salish Sea, William I. Atlas, Natalie C. Ban, Jonathan W. Moore, Adrian M. Tuohy, Spencer Greening, Andrea J. Reid, Nicole Morven, Elroy White, William G. Housty, Jess A. Housty, Christina N. Service, Larry Greba, Sam Harrison, Katherine Ir Butts, Elissa Sweeney-Bergen, Donna Macintyre, Matthew R. Sloat, Katrina Connors

Institute Publications

Indigenous peoples of the Northern Pacific Rim have harvested salmon for more than 10,000 years, and Pacific salmon (Oncorhynchus spp.) form the foundation of social-ecological systems encompassing communities from California to Kamchatka and Northern Japan. Through continuous placed-based interdependence with salmon, Indigenous societies formed deliberate and well-honed systems of salmon management. These systems promoted the sustained productivity of salmon fisheries. In Canada and the United States, Indigenous sovereignty and resource stewardship were forcibly disrupted by colonial government authority. Despite the destructive impacts of colonization, Indigenous culture and knowledge are resurgent in Canada and the United States. Indigenous fishing technologies and …


Vignette 19: Invasive European Green Crab, Jeff Adams, Emily Grason, P. Sean Mcdonald, Allen Pleus, Jude Apple, Roger Fuller, Lucas Hart, Alexandra Simpson May 2021

Vignette 19: Invasive European Green Crab, Jeff Adams, Emily Grason, P. Sean Mcdonald, Allen Pleus, Jude Apple, Roger Fuller, Lucas Hart, Alexandra Simpson

Institute Publications

European green crab pose documented threats to cultured and wild shellfish, eelgrass, and shoreline habitats and ecosystems. Because they can prey on juvenile crabs and shellfish, dense populations of EGC in the Salish Sea region could put fisheries and aquaculture resources in peril. After Fisheries and Oceans Canada researchers reported an established EGC population in Sooke Basin, BC in 2012, the Washington Department of Fish and Wildlife (WDFW) worked with Washington Sea Grant (WSG) to secure Puget Sound Marine and Nearshore Grant Program funding and establish a volunteer-based early detection and monitoring program. WSG launched Crab Team in 2015 with …


Vignette 14: Eelgrass Wasting Disease, Olivia Graham, Morgan Eisenlord, Drew Harvell May 2021

Vignette 14: Eelgrass Wasting Disease, Olivia Graham, Morgan Eisenlord, Drew Harvell

Institute Publications

Rising seawater temperatures can increase the risk of disease outbreaks in many taxa. Pathogens are potentially the ultimate keystone species in that their small biomass can have massive impacts that ripple through ecosystems. Disease outbreaks can be particularly damaging when they affect ecosystem engineers, such as seagrasses. Outbreaks of wasting disease in seagrasses are one of a myriad of stressors associated with declining temperate and tropical seagrass meadows around the globe. Levels of eelgrass wasting disease are high in the San Juan Islands and Puget Sound. These increasing levels of disease are a threat to sustainability of eelgrass meadows, our …


Life History, Diet And Habitat Of The Federally Endangered Laurel Dace (Chrosomus Saylori), Shawna M. Fix May 2021

Life History, Diet And Habitat Of The Federally Endangered Laurel Dace (Chrosomus Saylori), Shawna M. Fix

Masters Theses

Laurel Dace (Chrosomus saylori) is a small, freshwater minnow that is endemic to headwater streams on Walden Ridge, Tennessee. The species was listed as federally endangered in 2011 and the U.S. Fish and Wildlife Service Recovery Plan indicates a need for life history, diet and habitat studies. A literature review, samples of archived specimens, and a surrogate species, the Tennessee Dace (Chrosomus tennesseensis), were used to describe the life history and diet of the Laurel Dace. A total of 370 Tennessee Dace were collected from Laurel Ford Branch, a stream 8.6 km from the Laurel Dace …


Climate-Driven Impacts On Himalayan Aquatic Biodiversity: A Case Study Involving Snowtrout (Cyprinidae: Schizothorax), Riri Wiyanti Retnaningtyas May 2021

Climate-Driven Impacts On Himalayan Aquatic Biodiversity: A Case Study Involving Snowtrout (Cyprinidae: Schizothorax), Riri Wiyanti Retnaningtyas

Graduate Theses and Dissertations

Monitoring biodiversity, to include its relative dispersal and contraction, has become a conservation task of great importance, particularly given the catastrophic and ongoing loss of habitat due to climate change. However, the timing, direction, and magnitude of these rates vary across taxa and ecosystems. Predicting specific impacts of climate change can thus be difficult and this, in turn, hampers management action. Metrics are needed to not only quantify contemporary requirements of species, but also predict potential distributions that fluctuate in lockstep with climate.

Montane ecosystems in the Himalayas are highly impacted by climate change, yet remain largely understudied due to …


Particle Tracking Reveals Pelagic Red Crabs As Indicators Of Climate-Driven Range Expansion In The California Current, Elizabeth Saraf May 2021

Particle Tracking Reveals Pelagic Red Crabs As Indicators Of Climate-Driven Range Expansion In The California Current, Elizabeth Saraf

Senior Honors Projects

Since their first recorded occurrence in 1859, Pelagic Red Crabs (PRC; Pleuroncodes planipes) have experienced increased frequency of episodic mass stranding events in the Eastern Pacific Ocean. PRC’s are transported over 1,000 km northward of their central domain from the coast of central Baja California, Mexico. They have been reported by the thousands on the beaches of central and southern California. Their presence influences the surrounding ecosystem and commercial fisheries because they are nutritional prey items and large predators change their diet to incorporate PRC’s when they are available. Seasonal coastal currents and the California Undercurrent transport warmer waters …


Effects Of Heavy Metal Pollution On The Antipredator Behavior Of Orangethroat Darters (Etheostoma Spectabile), Caleb S. O'Neal May 2021

Effects Of Heavy Metal Pollution On The Antipredator Behavior Of Orangethroat Darters (Etheostoma Spectabile), Caleb S. O'Neal

MSU Graduate Theses

Heavy metal pollution can have numerous negative impacts on stream fishes, including both lethal and sublethal effects. Because of the sensitivity of fishes to toxins, they are excellent environmental indicators of stream and watershed health. The Tri-State Mining District is a Superfund site located in parts of Missouri, Kansas and Oklahoma that offers a good opportunity to study sublethal effects of heavy metal pollutants on fish behavior. I observed the antipredator behavior of Orangethroat Darters (Etheostoma spectabile) from 3 streams that varied in the abundance of heavy metal pollutants. In the lab, darters from the most polluted site …


Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz Apr 2021

Evaluation Of Lake Sturgeon (Acipenser Fulvescens) Spawning Success On An Artificial Reef Constructed In The Kalamazoo River, Michigan, Jason P. Lorenz

Masters Theses

Since lake sturgeon Acipenser fulvescens monitoring began on the Kalamazoo River in 2009, there has been no documentation of survival past the egg stage. Spawning has occurred directly below Calkins Dam and, in 2016, an artificial spawning reef was constructed 275-m downstream to improve habitat and increase survival to the larval stage. The site was monitored with egg mats and larval drift nets to document evidence of successful natural reproduction of lake sturgeon one year prior to construction (2016) and three years post-construction of the artificial reef (2017-2019). Fertilized lake sturgeon eggs were captured all four years of the study …


Using Acoustic Telemetry To Analyze The Impacts Of Common Carp On Wild Rice Restoration, Franklin Zomer Mar 2021

Using Acoustic Telemetry To Analyze The Impacts Of Common Carp On Wild Rice Restoration, Franklin Zomer

All NMU Master's Theses

Wild rice (Zizania palustris) is an important cultural, spiritual, and dietary resource to Lake Superior Ojibwe. Common Carp (Cyprinus carpio) are an introduced species that negatively impact aquatic vegetation through changes in water quality, uprooting of plants, and consumption of seed. Acoustic telemetry was used to document Common Carp movements in Waishkey Bay in the upper St. Marys River, MI where wild rice habitat is present. Exclosures were established to measure the influence of carp presence on wild rice seeding success. Common Carp were observed to spend much of their time in Waishkey Bay but also …


Global Extinction Risk For Seahorses, Pipefishes And Their Near Relatives (Syngnathiformes), Riley A. Pollom, Gina M. Ralph, Caroline M. Pollock, Amanda C.J. Vincent Jan 2021

Global Extinction Risk For Seahorses, Pipefishes And Their Near Relatives (Syngnathiformes), Riley A. Pollom, Gina M. Ralph, Caroline M. Pollock, Amanda C.J. Vincent

Biological Sciences Faculty Publications

Few marine taxa have been comprehensively assessed for their conservation status, despite heavy pressures from fishing, habitat degradation and climate change. Here we report on the first global assessment of extinction risk for 300 species of syngnathiform fishes known as of 2017, using the IUCN Red List criteria. This order of bony teleosts is dominated by seahorses, pipefishes and seadragons (family Syngnathidae). It also includes trumpetfishes (Aulostomidae), shrimpfishes (Centriscidae), cornetfishes (Fistulariidae) and ghost pipefishes (Solenostomidae). At least 6% are threatened, but data suggest a mid-point estimate of 7.9% and an upper bound of 38%. Most of the threatened species are …