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Full-Text Articles in Aquaculture and Fisheries
Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown
Vaccines For Infection Salmon Anemia Virus, Nathan Edward Charles Brown
Electronic Theses and Dissertations
Infectious salmon anemia (ISA) virus is an emerging pathogen of fanned Atlantic salmon. Due to the massive economic losses inflicted by the ISA virus, effective measures to control future outbreaks are necessary. An attractive method for preventing ISA virus from infecting stocks of Atlantic salmon is vaccination. DNA vaccination is a proven cheap, effective means of protecting fish from aquatic viruses. In this thesis DNA vaccination of Atlantic salmon was investigated. Three different strains of ISA virus were cultured and purified by density gradient ultracentrifugation. ISA virus RNA was isolated and used to amplify full-length cDNAs of five ISA virus …