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California Polytechnic State University, San Luis Obispo

2010

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Articles 31 - 43 of 43

Full-Text Articles in Animal Sciences

Culturally Speaking: Beyond Yogurt, Phillip S. Tong May 2010

Culturally Speaking: Beyond Yogurt, Phillip S. Tong

Dairy Science

No abstract provided.


Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent As. Fujimoto, Rafael Jimenez-Flores, Daniel G. Peterson May 2010

Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent As. Fujimoto, Rafael Jimenez-Flores, Daniel G. Peterson

Animal Science

The MAC-T cell line has been used extensively to investigate bovine mammary epithelial cell function. A lactogenic phenotype is generally induced in this cell line by a combination of dexamethasone, insulin and prolactin and has typically been assessed by milk protein production. Few studies have focused on identifying other factors that may affect milk protein synthesis in the MAC-T cell line, and none have considered the lipid class distribution of MAC-T cells as a component of the lactogenic phenotype. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and has …


Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, Robert J. Delmore, Jr., J. M. Hodgen, B. J. Johnson Apr 2010

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, Robert J. Delmore, Jr., J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …


Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, R. J. Delmore, J. M. Hodgen, B. J. Johnson Apr 2010

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, R. J. Delmore, J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …


Mesenchymal Stem Cells Isolated From A Novel Equine Tissue Exhibit Embryonic Molecular Markers And Adipogenic Differentiation Potential, Jane Marie Isquith, Kelly Shaw, Karen Shaw, Daniel Peterson, Matthew Burd Apr 2010

Mesenchymal Stem Cells Isolated From A Novel Equine Tissue Exhibit Embryonic Molecular Markers And Adipogenic Differentiation Potential, Jane Marie Isquith, Kelly Shaw, Karen Shaw, Daniel Peterson, Matthew Burd

Animal Science

Mesenchymal stem cells (MSC) have been isolated from equine tissues, most notably adipose and bonemarrow. On the basis of ability for self-renewal, molecular marker expression and differentiation potential, we characterized MSCs isolated from a tissue not yet explored in the equine. After isolation, MSCs weremaintained in culture for 25 passages before senescence was observed. Reverse transcription-PCR revealedembryonic transcription factor Oct4 mRNA, as well as mRNA for fucosyltransferase 4 and ST3B-galactosid α 2,3 sialySSEA4, respylxanthine l ltransferase 2, two enzymes responsible for synthesis of embryonic surface markersSSEA1 and ectively. Treatment with insulin, dexamethasone, indomethacin andisobutylmethed to increased oil red O staining …


Lh Increases Insl3 Mrna Abundance In Ma-10 Leydig Cells, Mary Elizabeth Strong, Matthew Anderson Burd, Daniel Gunnar Peterson Apr 2010

Lh Increases Insl3 Mrna Abundance In Ma-10 Leydig Cells, Mary Elizabeth Strong, Matthew Anderson Burd, Daniel Gunnar Peterson

Animal Science

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoterin Leydig cells can be activated by cAMP through the transcription factor Nur77. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. MA-10 Leydig cells were treated with either 0 and 100 ng/mL LH for 3 and 6 hours in a 2x2 factorial …


Comparison Of The Effects Of Growth Hormone On Mac-T Cells And Primary Mammary Cells, Lisa Mcdonnell, Tasha Johnson, Daniel G. Peterson Apr 2010

Comparison Of The Effects Of Growth Hormone On Mac-T Cells And Primary Mammary Cells, Lisa Mcdonnell, Tasha Johnson, Daniel G. Peterson

Animal Science

The ability of growth hormone (GH) to increase milk yield through somatomedins is well characterized,however recent studies utilizing mammary epithelial cell (MEC) lines have indicated a possible direct effecton MEC. In the mammary alveolar cell-T (MAC-T) line, relative abundance of mRNA for two milk protein genes, α-lactalbumin and αS1-casein, and GH receptor was increased by GH. MAC-T cells were compared tobovine mammary tissue explants and primary MEC isolated from raw milk through culture in classicallactation medium (dexamethasone, insulin, prolactin) with 0 or 10 ng/mL GH. Explant and primary cellmRNA abundance showed a similar pattern to MAC-T indicating that the effects …


Identifying Alternative Waste Conversion Technologies For Use At California Polytechnic State University San Luis Obispo, Matthew R. Bono Mar 2010

Identifying Alternative Waste Conversion Technologies For Use At California Polytechnic State University San Luis Obispo, Matthew R. Bono

BioResource and Agricultural Engineering

This project was undertaken to identify and study alternative technologies for converting waste streams available on the Cal Poly San Luis Obispo campus into energy. As a large campus on the Central Coast of California Cal Poly not only produces lots of waste, but has limited options for its disposal. Furthermore, the campus is in the midst of an on-going green initiative, and more efficient use of its waste products would be a welcome development for the entire campus community. This report gathers information on waste streams originating at Cal Poly and information about waste to energy conversion technologies. The …


Culturally Speaking: The Year Of Sodium, Phillip S. Tong Feb 2010

Culturally Speaking: The Year Of Sodium, Phillip S. Tong

Dairy Science

No abstract provided.


The Presence Of Micropterus Salmoides (Largemouth Bass) Influences The Populations Of Rana Draytonii (California Red-Legged Frog) And Pseudacris Regilla (Pacific Treefrog) In Two Ponds In Santa Barbara County, California, Kenneth Lee Gilliland Feb 2010

The Presence Of Micropterus Salmoides (Largemouth Bass) Influences The Populations Of Rana Draytonii (California Red-Legged Frog) And Pseudacris Regilla (Pacific Treefrog) In Two Ponds In Santa Barbara County, California, Kenneth Lee Gilliland

Master's Theses

Alien fish have been implicated in the decline of Rana draytonii (California red-legged frog) and Pseudacris regilla (Pacific treefrog) populations. Micropterus salmoides (largemouth bass) is a common sport fish that has been introduced into the sympatric range of these two anuran species; however, no studies have investigated the individual influence that this alien fish has on these two species. I conducted M. salmoides control or eradication experiments during a four year study in two ponds within the Transverse Mountain Range of Santa Barbara County, California. Changes in the densities of all life stages of R. draytonii and P. regilla were …


Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho Jan 2010

Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho

Dairy Science

Current research on bioactive molecules in milk has documented health advantages of bovine milk and its components. Milk Phospholipids, selected for this study, represent molecules with great potential benefit in human health and nutrition. In this study we used confocal reflectance and multiphoton microscopy to monitor changes in skin morphology upon skin exposure to ultraviolet light and evaluate the potential of milk phospholipids in preventing photodamage to skin equivalent models. The results suggest that milk phospholipids act upon skin cells in a protective manner against the effect of ultraviolet (UV) radiation. Similar results were obtained from MTT tissue viability assay …


The Effects Of Zilpaterol Hydrochloride On Carcass Cutability And Tenderness Of Calf-Fed Holstein Steers, A. J. Garmyn, J. N. Shook, D. L. Vanoverbeke, J. L. Beckett, Robert J. Delmore, D. A. Yates, D. M. Allen, G. G. Hilton Jan 2010

The Effects Of Zilpaterol Hydrochloride On Carcass Cutability And Tenderness Of Calf-Fed Holstein Steers, A. J. Garmyn, J. N. Shook, D. L. Vanoverbeke, J. L. Beckett, Robert J. Delmore, D. A. Yates, D. M. Allen, G. G. Hilton

Animal Science

To evaluate the impact of zilpaterol hydrochloride (ZH) on carcass cutability and tenderness of calf-fed Holstein steers, calf-fed Holstein carcasses (n = 102) were selected from a pool of 2,300 steers that were fed 0 or 8.3 mg/kg (DM basis) of ZH. Zilpaterol hydrochloride was supplemented the last 20 d of the finishing period and withdrawn for 3 d before slaughter. Carcasses were selected based on carcass weight as well as predetermined USDA Yield grade categories. For tenderness evaluation, steaks from the strip loin, bottom round, and top round (n = 54 per subprimal) were aged for 14 or 21 …


Effects Of Zilpaterol Hydrochloride Feeding Duration On Beed And Calf-Fed Holstein Strip Loin Steak Color, H. R. Rogers, J. C. Brooks, M. C. Hunt, G. G. Hilton, D. L. Vanoverbeke, J. Killefer, T. E. Lawrence, R. J. Delmore, B. J. Johnson, D. M. Allen, M. N. Streeter, W. T. Nichols, J. P. Hutcheson, D. A. Yates, J. N. Martin, M. F. Miller Jan 2010

Effects Of Zilpaterol Hydrochloride Feeding Duration On Beed And Calf-Fed Holstein Strip Loin Steak Color, H. R. Rogers, J. C. Brooks, M. C. Hunt, G. G. Hilton, D. L. Vanoverbeke, J. Killefer, T. E. Lawrence, R. J. Delmore, B. J. Johnson, D. M. Allen, M. N. Streeter, W. T. Nichols, J. P. Hutcheson, D. A. Yates, J. N. Martin, M. F. Miller

Animal Science

ABSTRACT: Two studies using beef and calf-fed Holstein cattle were conducted to determine the effect of zilpaterol hydrochloride (ZH) supplementation on the color of strip loin steaks packaged in traditional and modified-atmosphere packaging. Select (USDA) strip loins were obtained from the carcasses of beef (n = 118) or calf-fed Holstein (n = 132) cattle fed ZH (6.8 g/ton on a 90% DM basis) for the last 0, 20, 30, or 40 d of feeding. One portion of the strip loin was moisture enhanced, cut into steaks, and packaged in an atmosphere containing 80% oxygen and 20% carbon dioxide. The remaining …