Life Sciences Commons^™

Vibmilk: Non-Intrusive Milk Spoilage Detection Via Smartphone Vibration, Yuezhong Wu, Wei Song, Yanxiang Wang, Dong Ma, Weitao Xu, Mahbub Hassan, Wen Hu

Research Collection School Of Computing and Information Systems

Quantifying the chemical process of milk spoilage is challenging due to the need for bulky, expensive equipment that is not user-friendly for milk producers or customers. This lack of a convenient and accurate milk spoilage detection system can cause two significant issues. First, people who consume spoiled milk may experience serious health problems. Secondly, milk manufacturers typically provide a “best before” date to indicate freshness, but this date only shows the highest quality of the milk, not the last day it can be safely consumed, leading to significant milk waste. A practical and efficient solution to this problem is proposed …

A Review Of Dielectric Barrier Discharge Cold Atmospheric Plasma For Surface Sterilization And Decontamination, Kolawole Adesina, Ta Chun Lin, Yue-Wern Huang, Marek Locmelis, Daoru Frank Han

Biological Sciences Faculty Research & Creative Works

Numerous investigations have shown that non-equilibrium discharges at atmospheric pressure, also known as "cold atmospheric plasma" (CAP) are efficient to remove biological contaminants from surfaces of a variety of materials. Recently, CAP has quickly advanced as a technique for microbial cleaning, wound healing, and cancer therapy due to the chemical and biologically active radicals it produces, known collectively as reactive oxygen and nitrogen species (RONS). This article reviews studies pertaining to one of the atmospheric plasma sources known as Dielectric Barrier Discharge (DBD) which has been widely used to treat materials with microbes for sterilization, disinfection, and decontamination purposes. To …

The Impacts Of Anthropogenic Activity And Climate Change On The Formation Of Harmful Algal Blooms (Habs) And Its Ecological Consequence, Zhangxi Hu, Aifeng Li, Zhun Li, Margaret R. Mulholland

OES Faculty Publications

No abstract provided.

Isolating And Characterizing Lipid Degrading Microbes From Human Decomposition Soils, Brooke Amara Talley, Allison R. Mason, Jennifer Debruyn

Chancellor’s Honors Program Projects

No abstract provided.

Soil Microbial Community: Understanding The Belowground Network For Sustainable Grassland Management, Y. G. Zhu, W. D. Kong, B. D. Chen, Z. B. Nan, P. Christie

IGC Proceedings (1997-2023)

1. In addition to the use of conventional methodologies in soil microbial research, molecular techniques are now being applied to gain insights into the soil microbial community;
2. Plant diversity can exert impacts on soil microbial diversity (through root activities and plant litter etc.), but may in itself be significantly altered by soil properties;
3. Soil microbial diversity largely determines the stability of soil ecosystems under biotic and abiotic perturbations.
4. Management of soil microbial diversity can only be achieved through better understanding their structures and functions.

Nitrite Cycling In The Primary Nitrite Maxima Of The Eastern Tropical North Pacific, Nicole M. Travis, Colette L. Kelly, Margaret R. Mulholland, Karen L. Casciotti

OES Faculty Publications

The primary nitrite maximum (PNM) is a ubiquitous feature of the upper ocean, where nitrite accumulates in a sharp peak at the base of the euphotic zone. This feature is situated where many chemical and hydrographic properties have strong gradients and the activities of several microbial processes overlap. Near the PNM, four major microbial processes are active in nitrite cycling: ammonia oxidation, nitrite oxidation, nitrate reduction and nitrite uptake. The first two processes are mediated by the nitrifying archaeal/bacterial community, while the second two processes are primarily conducted by phytoplankton. The overlapping spatial habitats and substrate requirements for these microbes …

Fine-Scale Genetic Structure In Rhizosphere Microbial Communities Associated With Chamaecrista Fasciculata (Fabaceae), Mahboubeh Hosseinalizadeh Nobarinezhad, Lisa E. Wallace

Biological Sciences Faculty Publications

Soil microbiota of the rhizosphere are an important extension of the plant phenotype because they impact the health and fitness of host plants. The composition of these communities is expected to differ among host plants due to influence by host genotype. Given that many plant populations exhibit fine-scale genetic structure (SGS), associated microbial communities may also exhibit SGS. In this study, we tested this hypothesis using Chamaecrista fasciculata, a legume species that has previously been determined to have significant SGS. We collected genetic data from prokaryotic and fungal rhizosphere communities in association with 70 plants in an area of …

Effects Of Continuous In Situ Low-Dose Ionizing Radiation On Microorganisms, Molly E. Wintenberg

All Dissertations

Precise detection and monitoring of nuclear fuel cycle, enrichment, and weapon development activities are critical for supporting warfighter preparation in chemical, biological, radiological, nuclear, and explosives (CBRNE) operations, clandestine activities, and nuclear compliance. A biological sensing system could serve as an alternative to traditional detection methods by using organic material naturally present in the environment to discreetly detect residual trace nuclear material. Microorganisms provide an optimal platform for an alternative sensing system; however, their response to low levels of ionizing radiation is poorly characterized. Combining the power of next-generation sequencing and transcriptomic analysis, this dissertation takes an approach to obtain …

Microbiological Contamination Associated With The Proximity Of A Refuse Dumpsite To A River Situated In Okada, Edo State, Nigeria, Ebere J. Okafor-Elenwo, Odaro S. Imade, Osazee E. Izevbuwa

Journal of Bioresource Management

This study was performed to evaluate the potential contamination of the Okponha river situated near a dumpsite in Okada, Edo State, Nigeria. Water samples were collected and analyzed for bacteriological and parasitological quality using standard procedures. Isolation and enumeration of bacterial colonies were performed by pour plate technique and the isolated bacteria were identified by standard phenotypic tests. Helminths and protozoa were screened by the direct smear technique. The values of HPC (3.79 ± 0.12 log₁₀ CFU/ml) and TCC (2.20 ± 0.14 log₁₀ CFU/ml) obtained from the river water samples exceeded WHO and NAFDAC recommended limits (≤ 2 …

Soil Microbial Community: Understanding The Belowground Network For Sustainable Grassland Management, Y. G. Zhu, W. D. Kong, B. D. Chen, Z. B. Nan, P. Christie

IGC Proceedings (1997-2023)

Key points

1. In addition to the use of conventional methodologies in soil microbial research, molecular techniques are now being applied to gain insights into the soil microbial community;

2. Plant diversity can exert impacts on soil microbial diversity (through root activities and plant litter etc.), but may in itself be significantly altered by soil properties;

3. Soil microbial diversity largely determines the stability of soil ecosystems under biotic and abiotic perturbations.

4. Management of soil microbial diversity can only be achieved through better understanding their structures and functions.

Effects Of Probiotic Supplementation For Piglets In A Nursery: A Meta-Analysis Ofcontrolled Randomized Studies, Isabella Cristina De Castro Lippi, Larissa Braganholo Vargas, Fabiana Ribeiro Caldara, Henrique Biasotto Morais, Jean Kaique Valentim, Janaína Palermo Mendes

Turkish Journal of Veterinary & Animal Sciences

The objective of this article was to evaluate the effect of probiotic addition on average daily gain and feed conversion on the final piglet nursery phase. This study used a systematic review by meta-analysis to contrast the effect of piglets supplementation without antibiotics (negative control) and with antibiotics (positive control). These results archived a dichotomy that suggests a need for different statistical treatments to evaluate these outcomes obtained by articles. Because of this, a metaanalysis evaluation is justified to identify the animal performance on probiotic or antibiotic supplementation conditions. The use of probiotics improved the feed conversion in the experiments …

Influence Of Seasonality And Agricultural Practices On Soil Microbes In Kamishihoro, Japan, Y. Madegwa, Y. Uchida

IGC Proceedings (1997-2023)

Soil microbes are important for maintenance of soil fertility and ecosystem functions. Agricultural practices such as land use, seasonality and fertilizer application effect the soil microbial community structure. However, the effect of these management practices on soil microbes and related functions, especially in one specific region, is still not clear.

Therefore, the study was conducted in Kamishihoro, Japan, to determine effect of land use (cropland, grassland), season (summer, spring) and fertilizer (anaerobic digestate, control) on soil microbial community and functions. Experimental procedure included DNA extraction, quantification, purification, amplification of 16SrRNA (bacterial gene) and Next Generation Sequencing (Ion PGM). Data analysis …

The Genetic Architecture Of Sexual Dimorphism In The Moss, Leslie M. Kollar, Scott Kiel, Ashley J. James, Cody T. Carnley, Danielle N. Scola, Taylor N. Clark, Tikahari Khanal, Todd Rosenstiel, Elliott T. Gall, Karl Grieshop, Stuart F. Mcdaniel

Biology Faculty Publications and Presentations

A central problem in evolutionary biology is to identify the forces that maintain genetic variation for fitness in natural populations. Sexual antagonism, in which selection favours different variants in males and females, can slow the transit of a polymorphism through a population or can actively maintain fitness variation. The amount of sexually antagonistic variation to be expected depends in part on the genetic architecture of sexual dimorphism, about which we know relatively little. Here, we used a multivariate quantitative genetic approach to examine the genetic architecture of sexual dimorphism in a scent-based fertilization syndrome of the moss We found sexual …

Predicting, Monitoring And Controlling The Growth Of Listeria In Complex Food Matrices, Laura Mary Nyhan

Theses

Listeria monocytogenes is a foodborne pathogen which is a significant challenge in food production, particularly for ready-to-eat (RTE) products. Incidence of Listeria in RTE foods can be reduced by the application of intelligent combinations of preservative factors or hurdles, while data quantifying the efficacy of hurdle combinations can be exploited and used in the area of predictive microbiology. Traditional culture-based techniques, such as viable plate counts, are commonly used to monitor the growth/survival of microorganisms in foods, however these methods are tedious, time-consuming and due to their destructive nature, are associated with low reproducibility and high variability. Therefore, rapid, non-invasive …

Carbon Dynamic In The Decomposer Subsystem Of The Leymus Chinensis Grassland In Northeastern China, Yong Wang, Jixun Guo

IGC Proceedings (1997-2023)

No abstract provided.

Antimicrobial Properties Of An Unknown Microorganism Isolated From The Local Environment, Danielle Duryea

Undergraduate Honors Thesis Projects

Bacterial species that cause disease can usually be combatted with antibiotics; but as the years pass, more and more bacterial pathogens are becoming resistant to these treatments. In fact, the Center for Disease Control has identified eighteen classes of bacteria ranging from urgent to concerning threats due antibiotic resistance (2019), warning the advance of an antibiotic resistance crisis in which untreatable bacterial infections will become a leading cause of death (Bennadi, 2014). The Small World Initiative is a program created in 2012 at Yale University to address the antibiotic crisis through a crowdsourcing effort where undergraduate students are encouraged to …

Soil Microbial Seasonal Community Dynamics In Response To Cover Crop And Phosphorus Fertilizer Usage In A No-Till Corn-Soybean System In 2018, C. L. Stewart, L. M. Starr, N. O. Nelson, K. L. Roozeboom, G. J. Kluitenberg, D. R. Presley, P. J. Tomlinson

Kansas Agricultural Experiment Station Research Reports

This study examined microorganism community composition in plots managed with and without cover crops and three contrasting phosphorous (P) fertilizer manage­ment techniques in a no-till corn-soybean system. This work was performed in the spring and fall of 2018 at the Kansas Agricultural Watershed Field Laboratory (KAW), Manhattan, KS. The study design was a 2 × 3 complete block factorial design with three replications, with cover crop presence or absence and three levels of P fertilizer management (control, fall broadcast, and spring injected). To examine microorganism community composition, phospholipid fatty acid (PLFA) analysis was used. Only the main effect of cover …

Microbial Energy Storage And Antimicrobial Effects Of Metal Nanoparticles, Octavia Allen

West Chester University Master’s Theses

With the use of Microbial Electrolysis Cells (MECs) and anaerobic digester (AD) designs, wastewater treatment could be more effectively processed. Inoculation of anaerobic microorganisms, methanogens specifically, can help convert organic matter in wastewater metabolizing it to produce CH₄. Most MECs are stationary and are not actively mixed to promote expedited CH₄ production. The objective of this work was to design Wind-Actuated Vibrating Electrochemical (WAVE) digesters and utilize electrical current to increase CH₄ production rates generated by anaerobic microorganisms in MECs, optimizing efficiency of wastewater treatment. It was hypothesized WAVE mixed digesters will enhance conversion of wastewater …

Msa: Dust As An Ecosystem Driver: Determining The Ecosystem Consequences Of Cross-System Subsidies Of Nutrients And Microorganisms In Dusts, Janice Brahney

Funded Research Records

No abstract provided.

Inevitable Future: Space Colonization Beyond Earth With Microbes First, Jose Lopez, Raquel S. Peixoto, Alexandre S. Rosado

Biology Faculty Articles

Based on modern microbiology, we propose a major revision in current space exploration philosophy and planetary protection policy, especially regarding microorganisms in space. Mainly, microbial introduction should not be considered accidental but inevitable. We hypothesize the near impossibility of exploring new planets without carrying and/or delivering any microbial travelers. In addition, although we highlight the importance of controlling and tracking such contaminations—to explore the existence of extraterrestrial microorganisms—we also believe that we must discuss the role of microbes as primary colonists and assets, rather than serendipitous accidents, for future plans of extraterrestrial colonization. This paradigm shift stems partly from the …

Going Back To The Soil: An Integrated Approach To Farming., Renée V. Christie

Theses and Dissertations (Comprehensive)

Agricultural productivity is often constrained by nutrient availability; as such, copious amounts of synthetic fertilizers are applied to maintain productivity. However, the intensive use of synthetic fertilizers has reduced the capacity of the soil to carry out crucial roles such as nutrient cycling because of shifts in the microbial community composition and structure. In addition, much of the applied synthetic fertilizers become lost to the environment through run-off, which contributes to soil degradation. With the increasing demand on agricultural systems to provide food and fibre and the adverse impacts of agricultural production on the soil resource, amendments that support soil …

Microbial Diversity In Urban Environments: Concern For Antibiotic Resistance, Ality Aghedo, Mangala Tawde, Nazrul I. Khandaker

Publications and Research

We are almost oblivious to the presence of microorganisms in our daily lives, but they exist and come into contact with us all the time. While some bacteria are harmless, and even beneficial, other bacteria can cause infections. A common treatment to bacterial infections is antibiotics and the success of an antibiotic depends on the resistance of the bacteria to the antibiotic. We conducted experiments to identify the types of bacteria that can be found on surfaces or within soil environments that come into contact with a vast number of people on a daily basis and how this can affect …

The Forensic Characterization Of Bacterial And Fungal Organisms In Traditional Chinese Medicine, Julia Grzymkowski, Christopher J. Ehrhardt, Justin L. Poklis, Michelle R. Peace

Undergraduate Research Posters

There has been an increase in use of Traditional Chinese Medicine (TCM) in the United States because they are less expensive and believed to be more effective with less adverse effects in comparison to traditional pharmaceutics. Therefore, sales have increased in the US, despite articles and case studies demonstrating the dangers, such as injury and death, related to TCM, stemming from improper labelling, toxic contaminants, and, in some cases, the presence of pathogenic bacteria. The aim of this study was to perform a survival experiment to demonstrate the importance of proper herbal brewing technique and to conduct a molecular and …

The Application Of Edta-Tris And Chlorhexidine In The Treatment Of Endometritis As A Replacement For Antibiotic Therapy In Cows, Igor Stojanov, Aleksandar Milovanovic, Dragana Ruzic-Muslic, Radomir Ratajac, Milica Zivkov Balos, Nevena Maksimovic, Jelena Apic

Turkish Journal of Veterinary & Animal Sciences

The treatment of reproductive disorders is based on the use of the available antibiotics or antiseptic agents. Prolonged or inadequate antibiotic treatment contributes to the development of antimicrobial resistances, while the use of antiseptics, particularly in high concentrations, leads to endometrial lesions and prolonging of the service period. The present study included monitoring of: 1) reproductive disorders of cows; 2) cytological, morphological, and bacteriological laboratory analyses; 3) susceptibility testing of isolated bacterial and reference strains to the EDTA-Tris (ethylene diamine tetraacetic acid-hydroxymethyl aminomethane) and chlorhexidine (CHX); and 4) an estimate of the time before onset of the new sexual cycle. …

Movement Of Cryptosporidium Parvum Oocysts Through Soils Without Preferential Pathways: Exploratory Test, Christophe J.G. Darnault, Zhenyang Peng, Chan Yu, Biting Li, Astrid R. Jacobson, Philippe C. Baveye

Plants, Soils, and Climate Faculty Publications

Groundwater contamination by oocysts of the waterborne pathogen Cryptosporidium parvum is a significant cause of animal and human disease worldwide. Although research has been undertaken in the past to determine how specific physical and chemical properties of soils affect the risk of groundwater contamination by C. parvum, there is as yet no clear conclusion concerning the range of mobility of C. parvum that one should expect in field soils. In this context, the key objective of this research was to determine the magnitude of C. parvum transport in a number of soils, under conditions in which fast and preferential …

Evaluation Of Filtration And Dna Extraction Methods For Environmental Dna Biodiversity Assessments Across Multiple Trophic Levels, Anni Djurhuus, Jesse Port, Collin Closek, Kevan Yamahara, Ofelia Romero-Maraccini, Kristine Walz, Dawn Goldsmith, Reiko Michisaki, Mya Breitbart, Alexandria Boehm, Francisco Chavez

Marine Science Faculty Publications

Metabarcoding of marine environmental DNA (eDNA), originating from tissue, cells, or extracellular DNA, offers the opportunity to survey the biological composition of communities across multiple trophic levels from a non-invasive seawater sample. Here we compare results of eDNA metabarcoding of multiple trophic levels from individual seawater samples collected from a kelp forest in Monterey Bay, California in order to establish methods for future cross-trophic level eDNA analysis. Triplicate 1 L water samples were filtered using five different 47 mm diameter membrane filters (PVDF, PES, GFF, PCTE, and NC) and DNA was extracted from triplicates of each filter-type using three widely-used …

Mass Spectrometric Analysis And Machine Learning Enable Microorganism Classification Based On Rna Posttranscriptional Modifications, Colin Christopher Aldrich

Legacy Theses & Dissertations (2009 - 2024)

RNA post-transcriptional modifications (PTMs) are dynamic features that can be up- or down-regulated by the health and metabolic state of a cell. These covalent modifications are installed and removed on RNA nucleosides by enzymes controlled by the activation and deactivation of specific genes. The goal of this research was to demonstrate that RNA PTMs can serve as a unique feature for the classification/identification of microorganisms. We utilized a scheme based on electrospray ionization mass spectrometry (ESI-MS) to obtain global PTM profiles from total RNA extracted from various microorganisms in optimal growth conditions as well as Salmonella typhimurium (S. typhimurium) spiked …

Atp Luminescence Assay As A Bioburden Estimator Of Biomass Accumulation In Caves, Janez Mulec, Andreea Oarga-Mulec

International Journal of Speleology

A commercially available adenosine triphosphate (ATP) detection system (Hygiena, USA), supported by cultivable microbial indicators, was used to estimate bioburden in different habitats in and outside show caves: air, water and solid surfaces. A strong positive correlation between ATP concentration expressed as Relative Light Units (RLU) and Colony-Forming-Units (CFU) was observed for swab samples from cave surfaces. In terms of ATP units, surfaces in a single cave system (Postojna Cave) varied considerably (240-1,258,800 RLU/ 20 cm²) and commonly exceeded the bioburden level of analogues on the surface (0-114,390 RLU/ 20 cm²). Cave sub-habitats were colonized by …

A Study On The Dissolution Of Autunite Minerals By Facultative Bacteria In Bicarbonate Media, Sandra C. Herrera Landaez

FIU Electronic Theses and Dissertations

Uranium (U) is a key contaminant at the Hanford site. The formation of uranyl-phosphate bearing minerals such as autunite as a result of tripoliphosphate injections has been used as a U immobilization strategy. Bacteria are known as key factors governing the fate and transport of soil contaminants. This research evaluated the interaction of facultative bacteria Shewanella Oneidensis MR-1 with autunite mineral in bicarbonate-amended media solutions. The concentration of several elements such as U, calcium (Ca) and phosphorous (P) released as a result of autunite mineral biodissolution were determined as a function of time; changes in cell density and protein assay …

Cultivation-Dependent Analysis Of Microorganisms Associated With Various Hydraulic Fracturing Fluids, Anthony Harrington

UNLV Theses, Dissertations, Professional Papers, and Capstones

The overarching goal of this study is to gain more insight to the microbial interactions associated with hydraulic fracturing by studying the culturable microorganisms present in various types of hydraulic fracturing fluids. Extraction of natural gas and other unconventional resources using hydraulic fracturing has increased in the last decade and very few studies have been conducted on the microorganisms associated with the various water types used in the process. From the very few published studies, the only cultivation-dependent method used involved determining the most probable number (MPN) of various metabolic groups, however the researchers failed to ask more in-depth questions …