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Articles 1 - 30 of 134
Full-Text Articles in Life Sciences
Using In Silico Tools To Analyze The 5ʹ Untranslated Regions Of The Alcohol Dehydrogenase Gene From Arabidopsis Thaliana And Omega Sequence, Reza Mohammadhassan, Tina Asadishad
Using In Silico Tools To Analyze The 5ʹ Untranslated Regions Of The Alcohol Dehydrogenase Gene From Arabidopsis Thaliana And Omega Sequence, Reza Mohammadhassan, Tina Asadishad
Makara Journal of Science
The 5ʹ ends of protein-encoding genes contain 5ʹ untranslated regions (5ʹUTRs), which can effectively participate in regulating gene expression. The 5ʹUTRs of Arabidospis thaliana–derived alcohol dehydrogenase gene (AtADH) and omega (Ω) sequence from tobacco mosaic virus (TMV) are the most effective enhancers in biotechnology. In this study, bioinformatics techniques were employed to analyze the characteristics of the above sequences. After 5ʹUTR sequence collection, the inner ribosome entrance sites; small RNA (sRNA) target sequences; nucleotide contents; and upstream start and stop codons, ORFs, and coding DNA sequences of the Ω sequence and AtADH 5ʹUTR were identified. Moreover, the …
Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Turkish Journal of Botany
In this study, it was aimed to determine the relationship between β carbonic anhydrase (βCA) gene expression levels and drought stress severity. For this purpose, the expression levels of six βCA genes in the roots and leaves of Arabidopsis thaliana were investigated under mild, moderate, and severe drought conditions. In addition, changes in the biomass, leaf water content, photosynthetic efficiency, CA enzyme activity, free proline content, and lipid peroxidation were also determined. The results showed that all of the β CA gene expression levels and CA enzyme activity increased in the leaf under mild drought conditions; however, the expression levels …
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Research in Biology
The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …
The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya
The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya
LSU Doctoral Dissertations
Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO2 and HCO3-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO2 diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Turkish Journal of Botany
Plants activate glutathione (GSH)-dependent detoxification pathways at biochemical and molecular levels under boron (B) toxicity. Sulfate uptake and transport are necessary for GSH biosynthesis in plants. Therefore, the transcriptional regulation of some sulfate transporters was determined in this study to clarify the importance of these transporters in leaf and root tissues of Arabidopsis thaliana under toxic B conditions. The expression level of SULTR1;3 was dramatically increased in leaf and root tissues under moderate and severe toxic B conditions, suggesting source-to-sink sulfate translocation under B toxicity. Stable expression levels of SULTR2;1, SULTR2;2, and low SULTR3;5 expression might restrict the sulfate movement …
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
Masters Theses
The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …
Examination Of Changes In Gene Family Size In Carnivorous Plants Through Genomic Analysis, Moonia Ammari
Examination Of Changes In Gene Family Size In Carnivorous Plants Through Genomic Analysis, Moonia Ammari
Theses, Dissertations and Culminating Projects
The traits associated with carnivory in plants have independently evolved within several orders of Angiosperm. Despite this lack of a common origin, carnivorous plants show many remarkably similar adaptations, suggesting that common evolutionary pressures may have shaped these species. Here, we hypothesize that one common trait carnivorous plants share is the number of genes associated with specific biological functions, such as nutrient transport. To test this hypothesis, we first identified genes and gene families in the model plant species Arabidopsis thaliana that are associated with previously characterized biological functions that may be important for plant carnivory. We then compared the …
Altered Collective Mitochondrial Dynamics In The Arabidopsis Msh1 Mutant Compromising Organelle Dna Maintenance, Joanna M. Chustecki, Ross D. Etherington, Daniel J. Gibbs, Iain G. Johnston
Altered Collective Mitochondrial Dynamics In The Arabidopsis Msh1 Mutant Compromising Organelle Dna Maintenance, Joanna M. Chustecki, Ross D. Etherington, Daniel J. Gibbs, Iain G. Johnston
Department of Biochemistry: Faculty Publications
Mitochondria form highly dynamic populations in the cells of plants (and almost all eukaryotes). The characteristics and benefits of this collective behaviour, and how it is influenced by nuclear features, remain to be fully elucidated. Here, we use a recently developed quantitative approach to reveal and analyse the physical and collective ‘social’ dynamics of mitochondria in an Arabidopsis msh1 mutant where the organelle DNA maintenance machinery is compromised. We use a newly created line combining the msh1 mutant with mitochondrially targeted green fluorescent protein (GFP), and characterize mitochondrial dynamics with a combination of single-cell time-lapse microscopy, computational tracking, and network …
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Faculty Works
The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …
Application Of Synthetic Peptide Cep1 Increases Nutrient Uptake Rates Along Plant Roots, Sonali Roy, Marcus Griffiths, Ivone Torres-Jerez, Bailey Sanchez, Elizabeth Antonelli, Divya Jain, Nicholas Krom, Shulan Zhang, Larry M. York, Wolf-Rüdiger Scheible, Michael Udvardi
Application Of Synthetic Peptide Cep1 Increases Nutrient Uptake Rates Along Plant Roots, Sonali Roy, Marcus Griffiths, Ivone Torres-Jerez, Bailey Sanchez, Elizabeth Antonelli, Divya Jain, Nicholas Krom, Shulan Zhang, Larry M. York, Wolf-Rüdiger Scheible, Michael Udvardi
Agricultural and Environmental Sciences Faculty Research
The root system of a plant provides vital functions including resource uptake, storage, and anchorage in soil. The uptake of macro-nutrients like nitrogen (N), phosphorus (P), potassium (K), and sulphur (S) from the soil is critical for plant growth and development. Small signaling peptide (SSP) hormones are best known as potent regulators of plant growth and development with a few also known to have specialized roles in macronutrient utilization. Here we describe a high throughput phenotyping platform for testing SSP effects on root uptake of multiple nutrients. The SSP, CEP1 (C-TERMINALLY ENCODED PEPTIDE) enhanced nitrate uptake rate per unit root …
Involvement Of Poly(Adp-Ribose)Ylation (Parylation) In The Regulation Of Antioxidant Defense System In Arabidopsis Thaliana Under Salt Stress, Rengi̇n Özgür Uzi̇lday
Involvement Of Poly(Adp-Ribose)Ylation (Parylation) In The Regulation Of Antioxidant Defense System In Arabidopsis Thaliana Under Salt Stress, Rengi̇n Özgür Uzi̇lday
Turkish Journal of Botany
Posttranslational modifications (PTM) are one of the first responses of plants to environmental stress and involve changing the location and activity of proteins in the cell. Addition of poly(ADP-ribose) (PAR) to proteins, poly(ADP-ribose)ylation (PARylation), is a posttranslational modification resulting from the binding of ADP-ribose from NAD+ to target proteins by PAR polymerases (PARP). PARylation is involved in many physiological events in plants including abiotic and biotic stress response. The aim of this work was to understand involvement of PARylation in inducing enzymatic antioxidant defence and alternative electron sinks in response to salinity. For this purpose A. thaliana plants were treated …
Exploring Knockdown Phenotypes And Interactions Between Atad3 Proteins In Arabidopsis Thaliana, Eli S. Gordon
Exploring Knockdown Phenotypes And Interactions Between Atad3 Proteins In Arabidopsis Thaliana, Eli S. Gordon
Masters Theses
Mitochondria are required for a diverse array of cellular functions and processes. ATAD3 (ATPase family AAA domain containing protein 3) proteins are newly discovered mitochondrial membrane proteins in Arabidopsis thaliana. Homologous to ATAD3A in metazoans, Co-Immunoprecipitation/Mass spectrometry and genomic analysis identified a four ATAD3A homologues in A. thaliana. The four A. thaliana proteins are referred to as ATAD3A1 (At3g03060), ATAD3A2 (At5g16930), ATAD3B1 (At2g18330), and ATAD3B2 (At4g36580). Studies in metazoans indicate that ATAD3A localizes to Mitochondria-ER contact sites and is involved in a variety of processes required for proper mitochondrial function, but ATAD3A proteins are poorly defined in plants. …
Formins Control Dynamics Of F-Actin In The Central Cell Of Arabidopsis Thaliana, Mohammad F. Ali, Tomokazu Kawashima
Formins Control Dynamics Of F-Actin In The Central Cell Of Arabidopsis Thaliana, Mohammad F. Ali, Tomokazu Kawashima
Plant and Soil Sciences Faculty Publications
In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin …
The Influence Of Singlet Oxygen And Loss Of Function Of Fatty Acid Desaturase 7 In The Chloroplast On Aphid Resistance In Arabidopsis Thaliana, Hillary Donna Fischer
The Influence Of Singlet Oxygen And Loss Of Function Of Fatty Acid Desaturase 7 In The Chloroplast On Aphid Resistance In Arabidopsis Thaliana, Hillary Donna Fischer
Graduate Theses and Dissertations
Fatty Acid Desaturase 7 (FAD7) is a chloroplast-localized enzyme that alters the fatty acid content of photosynthetic membranes, and that negatively regulates plant defenses against aphids. Previous studies in the model organism Arabidopsis (Arabidopsis thaliana) have shown that loss-of-function mutations in FAD7 decrease population growth of the green peach aphid (GPA; Myzus persicae Sulzer). This study further characterized the effects of a fad7 null mutant on aphids, and investigated the role of reactive oxygen species (ROS), including singlet oxygen (1O2), in plant responses to aphid resistance in fad7 as well as in wild type plants and a mutant with heightened …
The Ear Motif In The Arabidopsis Mads Transcription Factor Agamous-Like 15 Is Not Necessary To Promote Somatic Embryogenesis, Sanjay Joshi, Christian Keller, Sharyn E. Perry
The Ear Motif In The Arabidopsis Mads Transcription Factor Agamous-Like 15 Is Not Necessary To Promote Somatic Embryogenesis, Sanjay Joshi, Christian Keller, Sharyn E. Perry
Plant and Soil Sciences Faculty Publications
AGAMOUS-like 15 (AGL15) is a member of the MADS domain family of transcription factors (TFs) that can directly induce and repress target gene expression, and for which promotion of somatic embryogenesis (SE) is positively correlated with accumulation. An ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif of form LxLxL within the carboxyl-terminal domain of AGL15 was shown to be involved in repression of gene expression. Here, we examine whether AGL15′s ability to repress gene expression is needed to promote SE. While a form of AGL15 where the LxLxL is changed to AxAxA can still promote SE, another form with a …
Identification And Expression Profiling Of Toxic Boron-Responsive Micrornas And Their Targets In Sensitive And Tolerant Wheat Cultivars, Doğa Seli̇n Kayihan, Emre Aksoy, Ceyhun Kayihan
Identification And Expression Profiling Of Toxic Boron-Responsive Micrornas And Their Targets In Sensitive And Tolerant Wheat Cultivars, Doğa Seli̇n Kayihan, Emre Aksoy, Ceyhun Kayihan
Turkish Journal of Agriculture and Forestry
Post transcriptional gene regulation in wheat cultivars caused by boron (B) toxicity has not been reported to date. In this study, two wheat cultivars Bolal-2973 (B-tolerant) and Atay-85 (B-sensitive) were compared with regard to the transcriptional regulation of miR319, miR172 and miR398 and their plausible target genes in order to address differences in their performances under high B levels. The expression levels of Cu/ZnSOD gene were found to be greater in Atay exposed to toxic B compared to Bolal, as verified by stable expression level of miR398. In wheat cultivars, both toxic B concentrations might cause an induction of leaf …
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
Turkish Journal of Botany
Recently, boron (B) has been found to form a complex with anthocyanin, which could be evidence for the B tolerance mechanisms that reduce free B in the leaf tissues of plants. However, the molecular mechanism of anthocyanin biosynthesis and transport has not been satisfactorily elucidated in plants exposed to toxic B. Therefore, the changes in expression levels of some of the phenylpropanoid pathway genes, early and late flavonoid biosynthetic genes, and transcription factors related to anthocyanin biosynthesis and transport were determined in Arabidopsis thaliana under B toxicity. Accordingly, 1 mM boric acid treatment did not cause any significant change in …
Construction And Analysis Of Genetic Regulatory Networks With Rna-Seq Data From Arabidopsis Thaliana, Tessa Kriz
Construction And Analysis Of Genetic Regulatory Networks With Rna-Seq Data From Arabidopsis Thaliana, Tessa Kriz
Dissertations, Master's Theses and Master's Reports
Reconstruction of gene regulatory networks (GRNs) is a fundamental aspect of genetic engineering and provides a deeper understanding of the biological processes of an organism. Two methods were implemented to reconstruct the gene regulatory networks of Arabidopsis thaliana under two treatments: methyl jasmonate (MeJa) and salicylic acid (SA). The Joint Reconstruction of multiple Gene Regulatory Networks (JRmGRN) method was utilized to construct a joint network for identifying hub genes common to both conditions in addition to networks specific to each condition. The Differential Network Analysis with False Discover Rate Control method constructed a network of connections unique to only one …
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Physiology Faculty Publications
In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In …
The Effects Of Environmental Stress On Arabidopsis Thaliana Establishment And Development, Paige Petty
The Effects Of Environmental Stress On Arabidopsis Thaliana Establishment And Development, Paige Petty
Student Research, Papers, and Creative Works
Arabidopsis thaliana is a model organism for plant research because it grows quickly, is easy to maintain, and findings from Arabidopsis can be easily extrapolated to crop species such as tomato, soybean, corn, and sorghum. Of particular interest is the process of photomorphogenesis, which promotes seedling establishment in soil. A rise in temperature causes stress for the seedling, which can inhibit photomorphogenesis and cause elongated hypocotyls that grow regardless of light intensity. Drought can also affect the rate of photosynthesis and limit seedling growth. In this project, we explored how the adult Arabidopsis plant is affected by delayed seedling establishment …
Genome-Wide Occupancy Of Polycomb Group Proteins And Chromatin Remodeler Splayed And Their Interplay In Arabidopsis, Jie Shu
Electronic Thesis and Dissertation Repository
The Polycomb group (PcG) proteins form two protein complexes, Polycomb repressive complex 1 (PRC1) and PRC2, which are key epigenetic regulators in eukaryotes. PRC2 represses gene expression by catalyzing trimethylation of histone H3 lysine 27 (H3K27me3). In Arabidopsis thaliana, CURLY LEAF (CLF) and SWINGER (SWN) are two major H3K27 methyltransferases, playing essential roles in plant growth and development. Despite their importance, genome-wide occupancy profiles of CLF and SWN have not yet been determined and compared. In this thesis, I generated transgenic lines expressing GFP-tagged CLF/SWN and determined the genome-wide distributions of CLF and SWN in Arabidopsis seedlings. I also …
The Effect Of Metal Composition And Particle Size On Nanostructure-Toxicity In Plants, Natalie Lynn Smith
The Effect Of Metal Composition And Particle Size On Nanostructure-Toxicity In Plants, Natalie Lynn Smith
MSU Graduate Theses
Silver nanoparticles (AgNPs) have consistently been shown to have a detrimental effect on bacteria, fungi, and plants. The interaction of AgNPs with plants has received considerable scientific attention, because it is potentially through plants that these structures can enter the food chain and bioaccumulate in humans and animals. To determine the effects of AgNPs on plants, Arabidopsis thaliana seedlings were chronically exposed to sublethal levels of AgNPs using a standardized method. To gain insight on mechanism of phytotoxicity, the seedlings were exposed to low concentrations of Ag+ (in the form of silver nitrate), AgNPs, or gold nanoparticles (AuNPs). To …
Expression Of The High-Affinity K+ Transporter 1 (Pphkt1) Gene From Almond Rootstock ‘Nemaguard’ Improved Salt Tolerance Of Transgenic Arabidopsis, Amita Kaundal, Devinder Sandhu, Marco Duenas, Jorge F. S. Ferreira
Expression Of The High-Affinity K+ Transporter 1 (Pphkt1) Gene From Almond Rootstock ‘Nemaguard’ Improved Salt Tolerance Of Transgenic Arabidopsis, Amita Kaundal, Devinder Sandhu, Marco Duenas, Jorge F. S. Ferreira
Plants, Soils, and Climate Faculty Publications
Soil salinity affects plant growth and development, which directly impact yield. Plants deploy many mechanisms to cope with, or mitigate, salt stress. One of such mechanism is to control movement of ions from root to shoot by regulating the loading of Na+ in the transpiration stream. The high-affinity K+ transporter 1 (HKT1) is known to play a role in the removal of Na+from the xylem and bring it back to the root. As almond is a salt-sensitive crop, the rootstock plays an important role in successful almond cultivation in salt-affected regions. We currently lack knowledge on the molecular mechanisms involved …
Characterization Of The Motion Of Cellulose Synthase Protein Complexes In The Plant Cell Membrane, Nina Zehfroosh
Characterization Of The Motion Of Cellulose Synthase Protein Complexes In The Plant Cell Membrane, Nina Zehfroosh
Doctoral Dissertations
The polysaccharide cellulose is the main component of plant cell walls, so it is the most abundant polymer on Earth. While it is widely used in industry due to its remarkable properties, such as renewability and biodegradability, its biosynthesis is still not well understood. The large transmembrane protein Cellulose Synthase Complex (CSC) is responsible for synthesizing cellulose by polymerizing UDP glucose into the constituent glucan chains of cellulose. In this project, I used variable angle epi-fluorescence microscopy (VAEM) in combination with single-particle tracking to characterize the motion of GFP labeled CSCs in the hypocotyl of Arabidopsis thaliana (A. thaliana …
The Mitochondrial Nad+ Transporter (Ndt1) Plays Important Roles In Cellular Nad+ Homeostasis In Arabidopsis Thaliana, Izabel De Souza Chaves, Elias Feitosa-Araujo, Alexandra Florian, David B. Medeiros, Paula Da Fonseca-Pereira, Lennart Charton, Elmien Heyneke, Jorge A. C. Apfata, Marcel V. Pires, Tabea Mettler-Altmann, Wagner L. Arajuo, H. Ekkehard Neuhaus, Ferdinando Palmieri, Toshihiro Obata, Andreas P. M. Weber, Nicole Linka, Alisdair R. Femie, Adriano Nunes-Nesi
The Mitochondrial Nad+ Transporter (Ndt1) Plays Important Roles In Cellular Nad+ Homeostasis In Arabidopsis Thaliana, Izabel De Souza Chaves, Elias Feitosa-Araujo, Alexandra Florian, David B. Medeiros, Paula Da Fonseca-Pereira, Lennart Charton, Elmien Heyneke, Jorge A. C. Apfata, Marcel V. Pires, Tabea Mettler-Altmann, Wagner L. Arajuo, H. Ekkehard Neuhaus, Ferdinando Palmieri, Toshihiro Obata, Andreas P. M. Weber, Nicole Linka, Alisdair R. Femie, Adriano Nunes-Nesi
Department of Biochemistry: Faculty Publications
Nicotinamide adenine dinucleotide (NAD+) is an essential coenzyme required for all living organisms. In eukaryotic cells, the final step of NAD+ biosynthesis is exclusively cytosolic. Hence, NAD+ must be imported into organelles to support their metabolic functions. Three NAD+ transporters belonging to the mitochondrial carrier family (MCF) have been biochemically characterized in plants. AtNDT1 (At2g47490), focus of the current study, AtNDT2 (At1g25380), targeted to the inner mitochondrial membrane, and AtPXN (At2g39970), located in the peroxisomal membrane. Although AtNDT1 was presumed to reside in the chloroplast …
Moderate Level Of Toxic Boron Causes Differential Regulation Of Micrornas Related To Jasmonate And Ethylene Metabolisms In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Moderate Level Of Toxic Boron Causes Differential Regulation Of Micrornas Related To Jasmonate And Ethylene Metabolisms In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Turkish Journal of Botany
Earlier our colleagues detected that the genes related to jasmonate (JA), ethylene, and cell wall modification were significantly regulated under boron (B) toxicity in wheat. Determination of regulation mechanisms of these novel genes under B toxicity is very important in Arabidopsis thaliana as a model plant. As key regulators, the microRNAs (miRNAs) regulate gene expression at the posttranscriptional level and respond to numerous abiotic stresses in plants. In this study, expression levels of miRNAs such as miR159, miR172, miR319, and miR394 targeting JA and ethylene-related transcription factors and also miR397 targeting laccase were determined in Arabidopsis thaliana under toxic B …
Arabidopsis Thaliana Transformed With Streptanthus Farnsworthianus Metal Tolerance Protein 1 (Sfmtp1) Or The Native A. Thaliana Metal Tolerance Protein 1 ( Atmtp1) Cdna And The Effects On Sod Enzyme Activity Upon Nickel Treatment, Selina Urbina Fernandez
Theses and Dissertations
Superoxide Dismutase enzyme activity was analyzed in transgenic Arabidopsis thaliana, a non-accumulator weed plant species of the Brassicaceae family, that was transformed with Streptanthus farnsworthianus Metal Tolerance Protein1 (SfMTP1) or A. thaliana Metal Tolerance Protein 1 (AtMTP1 ). The transgenic A. thaliana was exposed to a 12 day treatment of 100 ?M Nickel Acetate in 1/10 X Hoaglands solution in hydroponics. Analysis of Super Oxide Dismutase activity determined that transgenic A. thaliana transformed with the AtMTP1 cDNA and treated with 100 µM Nickel showed a significant increase in Super Oxide Dismutase activity compared to A. thaliana …
Desert Plant Bacteria Reveal Host Influence And Beneficial Plant Growth Properties, Abdul Aziz Eida, Maren Ziegler, Feras F. Lafi, Craig T. Michell, Christian R. Voolstra, Heribert Hirt, Maged M. Saad
Desert Plant Bacteria Reveal Host Influence And Beneficial Plant Growth Properties, Abdul Aziz Eida, Maren Ziegler, Feras F. Lafi, Craig T. Michell, Christian R. Voolstra, Heribert Hirt, Maged M. Saad
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© 2018 Eida et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Deserts, such as those found in Saudi Arabia, are one of the most hostile places for plant growth. However, desert plants are able to impact their surrounding microbial community and select beneficial microbes that promote their growth under these extreme conditions. In this study, we examined the soil, rhizosphere and endosphere bacterial communities of four native desert plants Tribulus terrestris, Zygophyllum simplex, …