Life Sciences Commons^™

Selection Of Efficient Taq Dna Polymerase To Optimize T-Dna Genotyping Method For Rapid Detection Of Mutant Arabidopsis Thaliana Plants, Inna B. Chastukhina, Liliia R. Nigmatullina, Lia R. Valeeva, Eugene V. Shakirov

Biological Sciences Faculty Research

Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other multicellular organisms, research in the model plant Arabidopsis thaliana takes advantage of short generation time and an ever increasing arsenal of genetic and transgenic tools, including large collections of T-DNA knockout and activation lines. The availability of thousands of publicly available transgenic Arabidopsis lines provides a unique opportunity to address a number of important biological questions. However, identification of individual T-DNA mutant plants from a pool of seeds provided by a biological stock distribution center remains a …