Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Publication
- Publication Type
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
Microbial Impacts On Endocrine Disrupting Contaminants: Las Vegas Wash And Lake Mead, Nevada, Susanna May Blunt
Microbial Impacts On Endocrine Disrupting Contaminants: Las Vegas Wash And Lake Mead, Nevada, Susanna May Blunt
UNLV Theses, Dissertations, Professional Papers, and Capstones
International concern over endocrine disrupting chemicals (EDCs) has become heightened in recent years as more studies reveal their persistence in the environment and their detrimental effects on wildlife. However, little is known about the role of microorganisms in the fate and transport of these compounds in surface waters. Las Vegas Wash, a stream flowing into Lake Mead and fed primarily by treated wastewater effluent, provided a unique experimental system in which to study the role microorganisms play in the dispersal of these compounds in aquatic systems. Samples were collected from the Las Vegas Wash downstream of the Las Vegas Valley's …
Mutant Alcohol Dehydrogenase Leads To Improved Ethanol Tolerance In Clostridium Thermocellum, Steven D. Brown, Adam M. Guss, Tatiana V. Karpinets, Jerry M. Parks
Mutant Alcohol Dehydrogenase Leads To Improved Ethanol Tolerance In Clostridium Thermocellum, Steven D. Brown, Adam M. Guss, Tatiana V. Karpinets, Jerry M. Parks
Dartmouth Scholarship
Clostridium thermocellum is a thermophilic, obligately anaerobic, Gram-positive bacterium that is a candidate microorganism for converting cellulosic biomass into ethanol through consolidated bioprocessing. Ethanol intolerance is an important metric in terms of process economics, and tolerance has often been described as a complex and likely multigenic trait for which complex gene interactions come into play. Here, we resequence the genome of an ethanol-tolerant mutant, show that the tolerant phenotype is primarily due to a mutated bifunctional acetaldehyde-CoA/alcohol dehydrogenase gene (adhE), hypothesize based on structural analysis that cofactor specificity may be affected, and confirm this hypothesis using enzyme assays. …