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Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Pasteurella spp and other aerobic bacterial agents from the lungs of sheep and goats with pneumonia and to identify Pasteurella multocida and Mannheimia haemolytica by both culture methods and polymerase chain reaction (PCR). In addition, mouse pathogenecity tests were carried out on suspected P. multocida isolates. In the examination of lung samples collected from sheep, 15 (4.3%) P. multocida and 8 (2.3%) M. haemolytica strains were isolated and identified. The numbers of species identified in the goat samples were 1 (0.7%) for P. multocida and 6 (4%) for M. haemolytica. The differences …
In Vitro And In Vivo Effects Of Serine And Threonine On Follicle Growth, Differentiation And Atresia, Hüseyi̇n Baki̇ Çi̇ftçi̇
In Vitro And In Vivo Effects Of Serine And Threonine On Follicle Growth, Differentiation And Atresia, Hüseyi̇n Baki̇ Çi̇ftçi̇
Turkish Journal of Veterinary & Animal Sciences
This work was designed to test the effect of serine and threonine on follicle growth, differentiation and atresia under in vitro and in vivo conditions. Two experiments were conducted. In the first experiment, follicles 90-120 µm in diameter, from 21-day old mice, were dissected mechanically and cultured individually for 7 days. Follicles were subjected to 4 different treatments. Follicles in medium without amino acids and gonadotrophins were assigned as control groups. Follicles in medium with PMSG and without amino acids constituted a positive control whereas the other treatments were test groups. Every 24 h follicle diameters were measured and the …
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
Lungs from 8222 cattle slaughtered at an abattoir in Elazığ were examined macroscopically, and pneumonia was detected in 500 (6.1%) lungs. These samples were inoculated onto blood agar supplemented with 7% sheep blood for isolation of bacterial agents. A polymerase chain reaction (PCR) based upon the use of species-specific primers was carried out on DNA samples extracted from suspected Pasteurella spp. isolates. In addition, a mouse inoculation test was carried out on suspected Pasteurella multocida isolates. A total of 311 (62.2%) bacterial agents were isolated from the lung samples and were identified as 7.2% Staphylococcus aureus, 6% Pasteurella multocida, 6% …
Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan
Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan
Turkish Journal of Veterinary & Animal Sciences
We investigated the effects of drop volume on the development of mouse presumptive zygotes to the completely hatched blastocyst stage. For this aim, we established 3 experimental groups. In the first group, we cultured hybrid presumptive zygotes to the hatched blastocyst stage in 10 presumptive zygotes per 5, 10, 20, 40 and 50 µl drops. In the second group, we used microinjected hybrid zygotes for culture. In the last group, we cultured CD-1 mouse presumptive zygotes to the hatched blastocyst stage. There was no statistical difference in the first group. In contrast, the data demonstrated that there were significant differences …