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Full-Text Articles in Life Sciences
Effect Of Concrete Pond And Net-Cage Culture Systems On Growth Performance Andhaematological Parameters Of Siberian Sturgeon (Acipenser Baerii), Selçuk Duman
Turkish Journal of Veterinary & Animal Sciences
The effect of concrete pond and net-cage culture systems on growth and haematological indices of the Siberian sturgeon (Acipenser baerii) were studied in a 90-day experiment. A total of 300 fish with a stocking density of 12.6 ± 0.37 kg/m2 (initial average weight 1140 ± 53.8 g) were stocked in floating net-cages. Also, 276 fish with a stocking density of 12.6 ± 0.29 kg/m2 and an initial average weight of 1097 ± 72.6 g were stocked in concrete ponds. Throughout the study, the average weight, mean length, and haematological parameters of the fish in each environment were recorded every 30 …
An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun
An Atypical Localization Of Bacillus Anthracis In A Cow's Milk And Udder, Mi̇tat Şahi̇n, Fati̇h Büyük, Özgür Çelebi̇, Mustafa Reha Coşkun
Turkish Journal of Veterinary & Animal Sciences
A 6-year-old Simental-Swiss Brown cross-bred cow, which aborted in the first trimester of the gestation and involuntarily presented anthrax bacilli around the udder, was subjected to this study. The cow was vaccinated with an attenuated anthrax vaccine (Bacillus anthracis 34 F2) 6 months ago and had normal physical examination findings. Samples taken 10 days after the abortion were representative of all possible spaces that could carry the bacteria such as milk, blood, environment, and swabs from rectum, udder and tits of an infected cow. Microbiological examination was carried out by staining the smears with Azure and in vitro culture of …
Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz
Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz
Turkish Journal of Biology
A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in …
Isolation Of Salmonella Spp. In Camel Sausages From Retail Markets In Aydın, Turkey, And Polymerase Chain Reaction (Pcr) Confirmation, Gökben Özbey, Fi̇li̇z Kök, Adi̇le Muz
Isolation Of Salmonella Spp. In Camel Sausages From Retail Markets In Aydın, Turkey, And Polymerase Chain Reaction (Pcr) Confirmation, Gökben Özbey, Fi̇li̇z Kök, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
A total of 100 camel sausage samples from different retail markets in Aydın, located in southwest Turkey, were examined for the presence of Salmonella spp. by culture method. After sausage samples was preenriched in buffered peptone water and incubated 18-20 h at 37 °C. were transferred to Rappaport-Vassiliadis broth and Selenite broth, respectively, and incubated at 42 and 37 °C, respectively. After 24 and 48 h of incubation, one loopful from each of the enriched broths was streaked onto plates of Salmonella-Shigella agar and xylose lysine deoxycholate agar and incubated at 37 °C for 24 h. A 572-bp fragment of …
Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Isolation Of Aerobic Bacterial Agents From The Lungs Of Sheep And Goats With Pneumonia And Detection Of Pasteurella Multocida And Mannheimia Haemolytica By Polymerase Chain Reaction, Gökben Özbey, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to isolate Pasteurella spp and other aerobic bacterial agents from the lungs of sheep and goats with pneumonia and to identify Pasteurella multocida and Mannheimia haemolytica by both culture methods and polymerase chain reaction (PCR). In addition, mouse pathogenecity tests were carried out on suspected P. multocida isolates. In the examination of lung samples collected from sheep, 15 (4.3%) P. multocida and 8 (2.3%) M. haemolytica strains were isolated and identified. The numbers of species identified in the goat samples were 1 (0.7%) for P. multocida and 6 (4%) for M. haemolytica. The differences …
In Vitro And In Vivo Effects Of Serine And Threonine On Follicle Growth, Differentiation And Atresia, Hüseyi̇n Baki̇ Çi̇ftçi̇
In Vitro And In Vivo Effects Of Serine And Threonine On Follicle Growth, Differentiation And Atresia, Hüseyi̇n Baki̇ Çi̇ftçi̇
Turkish Journal of Veterinary & Animal Sciences
This work was designed to test the effect of serine and threonine on follicle growth, differentiation and atresia under in vitro and in vivo conditions. Two experiments were conducted. In the first experiment, follicles 90-120 µm in diameter, from 21-day old mice, were dissected mechanically and cultured individually for 7 days. Follicles were subjected to 4 different treatments. Follicles in medium without amino acids and gonadotrophins were assigned as control groups. Follicles in medium with PMSG and without amino acids constituted a positive control whereas the other treatments were test groups. Every 24 h follicle diameters were measured and the …
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Isolation Of Bacterial Agents From The Lungs Of Cattle With Pneumonia And Detection Of Pasteurella Spp. By Polymerase Chain Reaction, Ayşe Kiliç, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
Lungs from 8222 cattle slaughtered at an abattoir in Elazığ were examined macroscopically, and pneumonia was detected in 500 (6.1%) lungs. These samples were inoculated onto blood agar supplemented with 7% sheep blood for isolation of bacterial agents. A polymerase chain reaction (PCR) based upon the use of species-specific primers was carried out on DNA samples extracted from suspected Pasteurella spp. isolates. In addition, a mouse inoculation test was carried out on suspected Pasteurella multocida isolates. A total of 311 (62.2%) bacterial agents were isolated from the lung samples and were identified as 7.2% Staphylococcus aureus, 6% Pasteurella multocida, 6% …
Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan
Effect Of Culture Medium Volume On The Development And Viability Of Microinjected Or Noninjected One-Cell Hybrid And Cd-1 Strain Mouse Embryos, Haydar Bağiş, Hande Odaman Mercan
Turkish Journal of Veterinary & Animal Sciences
We investigated the effects of drop volume on the development of mouse presumptive zygotes to the completely hatched blastocyst stage. For this aim, we established 3 experimental groups. In the first group, we cultured hybrid presumptive zygotes to the hatched blastocyst stage in 10 presumptive zygotes per 5, 10, 20, 40 and 50 µl drops. In the second group, we used microinjected hybrid zygotes for culture. In the last group, we cultured CD-1 mouse presumptive zygotes to the hatched blastocyst stage. There was no statistical difference in the first group. In contrast, the data demonstrated that there were significant differences …
Identification Of Salmonella Isolated From Freshwater Mussels (Unio Elongatulus Eucirrus Bourguignat, 1860) By Polymerase Chain Reaction, Engi̇n Şeker, Mustafa Sarieyyüpoğlu, Burhan Çeti̇nkaya
Identification Of Salmonella Isolated From Freshwater Mussels (Unio Elongatulus Eucirrus Bourguignat, 1860) By Polymerase Chain Reaction, Engi̇n Şeker, Mustafa Sarieyyüpoğlu, Burhan Çeti̇nkaya
Turkish Journal of Veterinary & Animal Sciences
In this study, 627 live freshwater mussels collected from the Koçkale and Pertek regions of Keban Dam Lake were examined for the presence of Salmonella spp. Homogenates prepared from the soft tissue of mussels, after pre-enrichment in Rappaport Vasilliadis, were inoculated onto Brilliant Green Phenol Red Lactose agar and Salmonella-Shigella selective media. Biochemical tests and DNA extraction were carried out on isolates suspected of containing Salmonella. From 19 (4.8%) out of 397 mussels collected in the Koçkale region, Salmonella spp. were isolated and identified, but the agent could not be detected in mussels obtained from the Pertek region. DNA extracted …
Determination Of Prevalence Of Paratuberculosis In Dairy Cattle By Polymerase Chain Reaction (Pcr), Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Basri̇ Ertaş, Hasan Öngör, İ. Yavuz Sezen, H. Basri̇ Gülcü
Determination Of Prevalence Of Paratuberculosis In Dairy Cattle By Polymerase Chain Reaction (Pcr), Burhan Çeti̇nkaya, Adi̇le Muz, Hasan Basri̇ Ertaş, Hasan Öngör, İ. Yavuz Sezen, H. Basri̇ Gülcü
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the prevalence of paratuberculosis in dairy cattle in ElazİÛ and the surrounding villages. A polymerase chain reaction (PCR) based on IS900, an insertion sequence specific to Mycobacterium avium subsp. paratuberculosis, was used to detect mycobacterial DNA in milk samples of 500 dairy cows. The detection limit of the PCR was assessed by spiking a known negative milk sample with a dilution series (1x10 9 to 10 bacteria/ml) of reference M. avium subsp. paratuberculosis culture). In the agarose gel examination of the PCR amplified products, approximately 50 bacteria/ml were detected. In the examination …
A Study On The Possibilities Of Obtaining Larva From Native Flat Oysters (Ostrea Edulis L. ) Living In The Black Sea And Larval Metamorphosis Stage, Orhan Uyan, Orhan Aral
A Study On The Possibilities Of Obtaining Larva From Native Flat Oysters (Ostrea Edulis L. ) Living In The Black Sea And Larval Metamorphosis Stage, Orhan Uyan, Orhan Aral
Turkish Journal of Zoology
In this research, the possiblities of obtaining larva from oysters ( Ostrea edulis L.) living in the Black Sea and their metamorphosis stage were investigated. There were no difficulties in obtaining larvae. The results of this investigation indicate that flat oyster ( Ostrea edulis L.) culture is possible in Black Sea conditions.
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Turkish Journal of Biology
This study was conducted to compare the suitability of three microalgal species (Tetraselmis chuii, Skeletonema costatum and Rhinomonas reticulata), both individually and in combination, as food for Penaeus indicus during larval culture. To determine the best feeding density, each alga was fed to protozoea 1 (PZI) larvae from 10 to 80 cells mL -1 . The best algal-cell density, promoting the highest survival rate, greatest growth and fastest larval development, was obtained at 60-70 cells mL -1 with a combination of T. chuii and S. costatum. Low cell densities (10-20 cells mL -1 ) were not effective. The diatom S. …