Life Sciences Commons^™

Engineering Exosomes To Specifically Target The Mitochondria Of Brain Cells, Xin Yan, Xinqian Chen, Zhiying Shan, Lanrong Bi

Michigan Tech Publications, Part 2

Mitochondrial dysfunction is associated with various health conditions, including cardiovascular and neurodegenerative diseases. Mitochondrial-targeting therapy aims to restore or enhance mitochondrial function to treat or alleviate these conditions. Exosomes, small vesicles that cells secrete, containing a variety of biomolecules, are critical in cell-to-cell communication and have been studied as potential therapeutic agents. Exosome-based therapy has the potential to treat both cardiovascular and neurodegenerative diseases. Combining these two approaches involves using exosomes as carriers to transport mitochondrial-targeting agents to dysfunctional or damaged mitochondria within target cells. This article presents a new technique for engineering brain-derived exosomes that target mitochondria and has …

Combined Purebred And Crossbred Genetic Evaluation Of Columbia, Suffolk, And Crossbred Lamb Birth And Weaning Weights: Systematic Effects And Heterogeneous Variances, Napoleón Vargas Jurado, David R. Notter, Joshua B. Taylor, Daniel J. Brown, Michelle R. Mousel, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Despite the benefits of crossbreeding on animal performance, genetic evaluation of sheep in the U.S. does not directly incorporate records from crossbred lambs. Crossbred animals may be raised in different environments as compared to purebreds. Systemic factors such as age of dam and birth and rearing type may, therefore, affect purebred and crossbred performance differently. Furthermore, crossbred performance may benefit from heterozygosity, and genetic and environmental variances may be heterogeneous in different breeds and their crosses. Such issues must be accounted for in a combined (purebred and crossbred) genetic evaluation. The objectives of this study were to i) determine the …

Reassessment Of Amblyscirtes Hegon (Hesperiidae) As A Complex Of Four Distinct Species Revealed By Genomic Analysis, Harry Pavulaan, Ricky Patterson, Nick V. Grishin

The Taxonomic Report of the International Lepidoptera Survey

After the discovery of a unique phenotype in the southern United States with a different ventral ground color than nominotypical Amblyscirtes hegon (Scudder, 1863), which occurs in the northeastern United States, genomic analysis revealed that A. hegon is a species complex. Phenotypic, genitalic, and genomic differences of the complex are presented here. Four species are identified: A. hegon; A. nemoris (W. H. Edwards, 1864), stat. rest.; A. matheri Patterson, Pavulaan & Grishin, sp. n. (TL: USA, Mississippi, Warren County); and A. gelidus Grishin, Patterson & Pavulaan, sp. n. (TL: USA, Michigan, Van Buren County).

Single Night Surveys Of Moth Communities Can Serve As Ultra-Rapid Biodiversity Assessments, Daniel P. Duran, Matthew Timar, Blaine Rothauser

School of Earth & Environment Faculty Scholarship

Biodiversity conservation decisions are typically based on limited data and resources. For this reason, there is great interest in surveying taxa that may allow for a rapid assessment of the biodiversity at a site. Numerous taxa have been proposed and utilized for rapid assessments that allow for such a survey in a matter of weeks or less. Herein, we test the idea that nocturnal moths have many of the characteristics that make them ideal for such surveys, such as relative ease of identification, strong ecological association with specific plant species and habitats, high alpha diversity, extended seasonal activity, and ease …

Oliver Agee Catfish Neurocranium, Charlotte R. Oliver, Kristina Agee, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 10.7 cm total length, [Catfish], collected by [Kerstetter].

Catfish Neurocranium 3-D Scan, Pamela Gonzalez, Jarod Johnson, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Visual Imagery Subcategories And Their Neural Associations, Miguel Ibarguren

Undergraduate Scholarship and Creative Works

This literature review compiles research findings related to the brain areas associated with various visual imagery subcategories and explores their contributions to them. Through a search procedure, four research projects were used to identify ten distinct brain areas that demonstrated greater involvement in visual imagery. The visual imagery subcategories discussed in these research projects, and investigated in this review, include scene construction, episodic memory, imagery vividness, and visuospatial imagery. Scene construction was found to utilize the parahippocampal gyrus, hippocampus, retrosplenial cortex, and posterior parietal cortex. Episodic memory involves the same areas as scene construction, but also includes the precuneus and …

Common Snook (Centropomus Undecimalis) Neurocranium, Ryan Robison, Lesley Wallace, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium length of 16.5 cm Common Snook (Centropomus undecimalis), collected from Dr. Kerstetter's Lab on 10/23 by Ryan Robison and Lesley Wallace.

Yellow Grouper (Epinephelus Awoara), Tyler Jurain, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Allergic Bronchopulmonary Aspergillosis: Radiological And Microbiological Profile Of Patients Presented In An Outpatient Pulmonary Clinic In A Developing Country, Nousheen Iqbal, Muhammad Irfan, Mustafa Bin Ali Zubairi, Maaha Ayub, Safia Awan, Kauser Jabeen, Ali Bin Sarwar Zubairi

Section of Pulmonary & Critical Care

There is limited data available about allergic bronchopulmonary aspergillosis (ABPA) in Pakistan. The aim of the study was to describe the radiological and microbiological profile of ABPA patients presenting to the outpatient pulmonary clinic of a tertiary care hospital in Karachi, Pakistan. A retrospective study was conducted on ABPA patients who presented to the pulmonary outpatient clinic at Aga Khan University Hospital, Karachi, Pakistan, from January 2017 to December 2019. Data was collected on microbiology and radiology features on predesigned proforma. A total of 7759 asthmatic patients presented at the outpatient pulmonology clinic during the study period. Of the 245 …

Jack Crevalle Neurocranium, Rodolfo A. Heredia, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.

Brown Bullhead Catfish (Ameiurus Nebulosus) Neurocranium, Reginald Leon Kempson Iii, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from [6.3] cm total length (30 cm) [Brown Bullhead Catfish (Ameiurus nebulosus)], collected from [Dr.Kerstetters lab] on [6/December/2023] by [Reginald Kempson III]

Barriers To Use Of Cross-Laminated Timber In Maine, Shane R. O'Neill

Forest Resources Faculty Scholarship

To increase understanding of both the adoption rate and in-state manufacturing of mass timber In Maine, the 131^st Legislature and Governor Mills passed LD 881, a resolve directing a study of the barriers facing cross-laminated timber In Maine and provide recommendations to promote their use in construction. This study was developed in response to the resolve. The study engaged 108 unique participants to define available training, education, and experiences across the stakeholders throughout the building lifecycle process in the state.

From this information, the following five recommendations are proposed:

1. Understand the policies and initiatives of other states to develop …

Snail Races: An Inquiry-Based Approach To Learn Invasive Species Ecology, Emma Grindle, Heather E. Bergan-Roller, Ashton Johnson, Grace Lunaburg, Jennifer A.H. Koop

Faculty Peer-Reviewed Publications

Inquiry-based lessons allow learning that is hands-on and student-driven, fostering engagement and retention of knowledge in any discipline. Here, we use this learning framework to engage students in exploring the effects of biotic and abiotic factors on animal dispersal as a means of connecting students to multiple ecological concepts. Instructors are provided with a guided lecture on key ecological concepts including the impacts of invasive species, dispersal mechanisms, and species interactions with the environment. Furthermore, the interactive lecture introduces students to the snail study system and explains the experimental process, which involves low-cost, readily available materials (e.g., kiddie pools). Students …

Conservation Agriculture And Cover Crop Adoption By Smallholder Farmers In Cambodia: Understanding Perceptions, Challenges, And Opportunities For Soil Improvement, Presley Nickens, David R. Ader, Max C. Miller, Ii, Pao Srean, Tom Gill, Sovanneary Huot

Agricultural and Environmental Sciences Faculty Research

Practical solutions for soil conservation are needed to ensure sustainable food production. Conservation agriculture and the use of cover crops are promising strategies for soil improvement in agricultural systems. These strategies are being promoted in Cambodia to address rapidly declining soil fertility; however, there is a lack of insight into the perceptions of Cambodian smallholders towards cover cropping within a conservation agriculture approach. A greater understanding of the utilization and perceptions of cover crops is needed to increase adoption and prevent further soil degradation. This study utilized a mixed methods approach with quantitative data from a farmer survey and qualitative …

Recognizing Power And Control When Planning Your Estate, Allan Vyhnalek

Cornhusker Economics

The older generations have spent their entire professional lives using the power and control that they accumulated through hard work and their long tenures. Having them retire and give up that control and power is difficult for some in those generations. Overall, it is challenging for them to give up control or power.

What Did Higher Profit Cow-Calf Producers Do To Be More Profitable?, Randy Saner

Center for Agricultural Profitability

Cow-calf producers are always looking at ways to cut costs and improve profits. Looking at recent data from the University of Minnesota Center for Financial Management, FINBIN Livestock Analysis for 2022 representing cow-calf operations from Nebraska, South Dakota, and North Dakota can provide producers with some ideas on ways to improve profitability.

Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John Vucetich, Jennifer A. Leonard, Robert K. Wayne

Michigan Tech Publications, Part 2

Effective population size estimates are critical information needed for evolutionary predictions and conservation decisions. This is particularly true for species with social factors that restrict access to breeding or experience repeated fluctuations in population size across generations. We investigated the genomic estimates of effective population size along with diversity, subdivision, and inbreeding from 162,109 minimally filtered and 81,595 statistically neutral and unlinked SNPs genotyped in 437 grey wolf samples from North America collected between 1986 and 2021. We found genetic structure across North America, represented by three distinct demographic histories of western, central, and eastern regions of the continent. Further, …

Gag Grouper (Mycteroperca Microlepis) Neurocranium, Sierra Potts, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

10 cm neurocranium from Gag Grouper, Mycteroperca microlepis, collected from Snapper Mike's on 05/11/2023 by Sierra Potts

Chromosome 10q2432 Variants Associate With Brain Arterial Diameters In Diverse Populations: A Genome-Wide Association Study, Minghua Liu, Farid Khasiyev, Sanjeev Sariya, Antonio Spagnolo-Allende, Danurys L Sanchez, Howard Andrews, Qiong Yang, Alexa Beiser, Ye Qiao, Emy A Thomas, Jose Rafael Romero, Tatjana Rundek, Adam M Brickman, Jennifer J Manly, Mitchell Sv Elkind, Sudha Seshadri, Christopher Chen, Saima Hilal, Bruce A Wasserman, Giuseppe Tosto, Myriam Fornage, Jose Gutierrez

Journal Articles

BACKGROUND: Brain arterial diameters (BADs) are novel imaging biomarkers of cerebrovascular disease, cognitive decline, and dementia. Traditional vascular risk factors have been associated with BADs, but whether there may be genetic determinants of BADs is unknown.

METHODS AND RESULTS: The authors studied 4150 participants from 6 geographically diverse population-based cohorts (40% European, 14% African, 22% Hispanic, 24% Asian ancestries). Brain arterial diameters for 13 segments were measured and averaged to obtain a global measure of BADs as well as the posterior and anterior circulations. A genome-wide association study revealed 14 variants at one locus associated with global BAD at genome-wide …

Convolutional Neural Network-Based Gene Prediction Using Buffalograss As A Model System, Michael Morikone

Dissertations and Doctoral Documents from University of Nebraska-Lincoln, 2023–

The task of gene prediction has been largely stagnant in algorithmic improvements compared to when algorithms were first developed for predicting genes thirty years ago. Rather than iteratively improving the underlying algorithms in gene prediction tools by utilizing better performing models, most current approaches update existing tools through incorporating increasing amounts of extrinsic data to improve gene prediction performance. The traditional method of predicting genes is done using Hidden Markov Models (HMMs). These HMMs are constrained by having strict assumptions made about the independence of genes that do not always hold true. To address this, a Convolutional Neural Network (CNN) …

Effects Of Dimerization On The Deacylase Activities Of Human Sirt2., Jie Yang, Nathan I Nicely, Brian P Weiser

Rowan-Virtua School of Osteopathic Medicine Faculty Scholarship

Human sirtuin isoform 2 (SIRT2) is an NAD+-dependent enzyme that functions as a lysine deacetylase and defatty-acylase. Here, we report that SIRT2 readily dimerizes in solution and in cells and that dimerization affects its ability to remove different acyl modifications from substrates. Dimerization of recombinant SIRT2 was revealed with analytical size exclusion chromatography and chemical cross-linking. Dimerized SIRT2 dissociates into monomers upon binding long fatty acylated substrates (decanoyl-, dodecanoyl-, and myristoyl-lysine). However, we did not observe dissociation of dimeric SIRT2 in the presence of acetyl-lysine. Analysis of X-ray crystal structures led us to discover a SIRT2 double mutant (Q142A/E340A) that …

Nonan Gaitprint: An Imu Gait Database Of Healthy Young Adults, Tyler M. Wiles, Madhur Mangalam, Joel H. Sommerfeld, Seung Kyeom Kim, Kolby J. Brink, Anaëlle Charles, Alli Grunkemeyer, Marilena Kalaitzi Manifrenti, Spyridon Mastorakis, Nicholas Stergiou, Aaron Likens

Journal Articles

An ongoing thrust of research focused on human gait pertains to identifying individuals based on gait patterns. However, no existing gait database supports modeling efforts to assess gait patterns unique to individuals. Hence, we introduce the Nonlinear Analysis Core (NONAN) GaitPrint database containing whole body kinematics and foot placement during self-paced overground walking on a 200-meter looping indoor track. Noraxon Ultium MotionTM inertial measurement unit (IMU) sensors sampled the motion of 35 healthy young adults (19–35 years old; 18 men and 17 women; mean ± 1 s.d. age: 24.6 ± 2.7 years; height: 1.73 ± 0.78 m; body mass: …

Examination Of The Prevalence And Behaviors Of Pathogenic And Spoilage Bacteria At Multiple Stages In The Meat Processing Chain, Samuel C. Watson

Dissertations and Doctoral Documents from University of Nebraska-Lincoln, 2023–

As long as there is food, there will also be bacteria that are capable of compromising the quality and safety of food. This dissertation aims to study bacteria capable of spoiling meat, causing illness, or both. The literature review summarizes the understanding of several bacterial pathogens, current issues in combating those pathogens in the food supply, and how spoilage Pseudomonas behave in the meat supply. Chapter 2 explored the impact of organic acid treatments on the spoilage microbiome of raw beef during extended storage. While Pseudomonas and Yersinia were both present in significant levels, Lactococcus was determined to be the …

Characterization Of Adipocyte Mechanical Properties And Stretch Control Of Mechanosignaling In Adipocytes, Tasneem Bouzid

Dissertations and Doctoral Documents from University of Nebraska-Lincoln, 2023–

Obesity has reached global epidemic levels in recent decades and is the leading risk factor for type-2 diabetes (T2D). Adipose tissue behaves as a link between obesity and T2D, with dysfunctional extracellular matrix (ECM) remodeling, adipokine secretion and lipid metabolism leading to insulin resistance. The extracellular mechanophysical milieus is understood to regulate adipocyte differentiation and function through activation of mechanosensory machinery and related pathways. Therefore, exploring the adipocytic response to mechanical loading can provide great pathophysiological insight into obesity and T2D. Adipose tissue is exposed to various compound forces due to weight-bearing and movement, and adipocyte stiffness influences stress distribution …

Factors Affecting Forage Quality And The Subsequent Response In Production And Energy Metabolism In Lactating Jersey Cows, Kassidy Kate Buse

Dissertations and Doctoral Documents from University of Nebraska-Lincoln, 2023–

Forage quality is the potential of a forage to produce a production response in animals. Because of how essential they are in dairy cattle rations, forages can have major influence on the behavior and production of dairy cattle. In experiment 1, the effect of forage fragility on the rumen environment and digestibility was evaluated. Increasing the inclusion of a less fragile forage led to greater rumen retention time, which resulted in lower rumen pH and greater NDF digestibility. Experiment 2 evaluated the effects of diets high in either corn silage or alfalfa haylage supplemented with either corn- or soybased protein …

Resolution Of The Tetrabothrius Jagerskioeldi Cryptic Species Complex Among Holarctic Alcidae (Charadriiformes): Cestodes Among Fraterculinae—Exploring Marine Diversity, Host Range, And Dynamic Oceanography In The Greater North Pacific, Eric P. Hoberg, Kaylen Marie Soudachanh, Svetlana K. Bondarenko

MANTER: Journal of Parasite Biodiversity

In the biosphere, limits for diversity among species, communities, and biomes are revealed through intensive and extensive field-based inventory and assembly of voucher specimens and associated informatics examined in a phylogenetic, historical, ecological, and biogeographic arena. Archival resources for specimens and information contribute to a cumulative view of faunal structure and assembly under a comparative umbrella. Ultimately, species definitions, and inclusive partitions among populations and lineages, are fundamental in articulating hypotheses that examine interactions about evolution, the nature of organisms, and the condition of environments across space and time. We conclude our proposals establishing species limits for tapeworms of the …

Microbiome-Enabled Genomic Selection Improves Prediction Accuracy For Nitrogen-Related Traits In Maize, Zhikai Yang, Tianjing Zhao, Hao Cheng, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Root-associated microbiomes in the rhizosphere (rhizobiomes) are increasingly known to play an important role in nutrient acquisition, stress tolerance, and disease resistance of plants. However, it remains largely unclear to what extent these rhizobiomes contribute to trait variation for different genotypes and if their inclusion in the genomic selection protocol can enhance prediction accuracy. To address these questions, we developed a microbiome-enabled genomic selection method that incorporated host SNPs and amplicon sequence variants from plant rhizobiomes in a maize diversity panel under high and low nitrogen (N) field conditions. Our cross-validation results showed that the microbiome-enabled genomic selection model significantly …

Dataset For The Incorporation Of Climate Change Into A Multiple Stressor Risk Assessment For The Chinook Salmon (Oncorhynchus Tshawytscha) Population In The Yakima River, Washington Usa, Wayne Landis, Chelsea J. Mitchell, John D. Hader, Rory Nathan, Emma E. Sharpe

Environmental Sciences Faculty and Staff Publications

Data files available below

This data set is in support of Landis et al (in press 2024). A key question in understanding the implications of climate change is how to integrate ecological risk assessments that focus on contaminants with the environmental alterations from climate projections. This article summarizes the results of integrating selected direct and indirect effects of climate change into an existing Bayesian network previously used for ecological risk assessment. The existing Bayesian network Relative Risk Model (BN-RRM) integrated the effects of organophosphate pesticides concentrations, water temperature, and dissolved oxygen levels on the Chinook salmon population in the Yakima …

Hypothesis And Theory: Do Trees "Release The Tension" In Rainwater? Surface Tension Reduction In Throughfall And Stemflow From Urban Trees, Benjamin J. Noren, Nicholas R. Lewis, Kelly Cristina Tonello, Anna Ilek, John T. Van Stan Ii

Biological, Geological, and Environmental Faculty Publications

Knowledge of the processes and impacts associated with the canopy's partitioning of rainfall into stemflow (water that drains to the base of tree stems) and throughfall (water that drips through gaps and from canopy surfaces) has expanded in recent years. However, the effect of canopy interactions on the fundamental physical properties of rainwater as it travels through the canopy to the soil, particularly surface tension, remains understudied. To discuss specific hypotheses within this context and their relevance to ecohydrological theory, the surface tension of rainwater samples was examined directly. Over a period of 9 months, open rainwater, throughfall and stemflow …