Life Sciences Commons^™

Distinct Peripheral Blood Rna Responses To Salmonella In Pigs Differing In Salmonella Shedding Levels: Intersection Of Ifng, Tlr And Mirna Pathways, Ting-Hua Huang, Jolita J. Uthe, Shawn M. D. Bearson, Cumhur Yusuf Demirkale, Dan Nettleton, Susan Knetter, Curtis Christian, Amanda Ramer-Tait, Michael J. Wannemuehler, Christopher K. Tuggle

Department of Food Science and Technology: Faculty Publications

Transcriptomic analysis of the response to bacterial pathogens has been reported for several species, yet few studies have investigated the transcriptional differences in whole blood in subjects that differ in their disease response phenotypes. Salmonella species infect many vertebrate species, and pigs colonized with Salmonella enterica serovar Typhimurium (ST) are usually asymptomatic, making detection of these Salmonella-carrier pigs difficult. The variable fecal shedding of Salmonella is an important cause of foodborne illness and zoonotic disease. To investigate gene pathways and biomarkers associated with the variance in Salmonella shedding following experimental inoculation, we initiated the first analysis of the whole …

Modeling Protein Expression And Protein Signaling Pathways, Donatello Telesca, Peter Muller, Steven Kornblau, Marc Suchard, Yuan Ji

COBRA Preprint Series

High-throughput functional proteomic technologies provide a way to quantify the expression of proteins of interest. Statistical inference centers on identifying the activation state of proteins and their patterns of molecular interaction formalized as dependence structure. Inference on dependence structure is particularly important when proteins are selected because they are part of a common molecular pathway. In that case inference on dependence structure reveals properties of the underlying pathway. We propose a probability model that represents molecular interactions at the level of hidden binary latent variables that can be interpreted as indicators for active versus inactive states of the proteins. The …

Juvenile Hormone Regulates Vitellogenin Gene Expression Through Insulin-Like Peptide Signaling Pathway In The Red Flour Beetle, Tribolium Castaneum, Zhentao Sheng, Jingjing Xu, Hua Bai, Fang Zhu, Subba R. Palli

Entomology Faculty Publications

Our recent studies identified juvenile hormone (JH) and nutrition as the two key signals that regulate vitellogenin (Vg) gene expression in the red flour beetle, Tribolium castaneum. Juvenile hormone regulation of Vg synthesis has been known for a long time in several insects, but the mechanism of JH action is not known. Experiments were conducted to determine the mechanism of action of these two signals in regulation of Vg gene expression. Injection of bovine insulin or FOXO double-stranded RNA into the previtellogenic, starved, or JH-deficient female adults increased Vg mRNA and protein levels, thereby implicating the pivotal role for …

Expertos Analizan Impacto Del Cambio Climático En Granos Básicos Y Sorgos Forrajeros En El Salvador, Kimberly Christiansen

INTSORMIL Impacts and Bulletins

En el evento participan profesionales de El Salvador, así como de las universidades de Kansas State y Purdue, de Estados Unidos.

Exponer y analizar los principales efectos derivados del cambio climático en los cultivos de El Salvador, así como el impacto que ha tenido la adopción de variedades forrajeras de sorgo en el país, son los objetivos principales de los foros realizados este miércoles por el Centro Nacional de Tecnología Agropecuaria y Forestal “Enrique Álvarez Córdova” (CENTA) con apoyo del Instituto Internacional de Sorgo, Mijo y otros granos (INTSORMIL).

Rentabilidad De La Introduccion De Nuevos Cultivares De Sorgo En La Industria Lactea De El Salvador: Estudio De Impacto Economico, Alexis H. Villacís, John H. Sanders

INTSORMIL Presentations

La introducción de nuevas tecnologías:

Propulsor del desarrollo agrícola.

Sorgos sensitivos:

Reserva o póliza de seguro

Introducidos recientemente desde los años 70 para la siembra en los valles en la segunda temporada.

Últimos 30 años introducción acelerada: Alimentación de aves y en el sector del ganado lechero.

Inversiones de CENTA y MAG.

Aumento de ingresos:

Mayor demanda de alimentos de alta calidad (carne, leche, queso, frutas y verduras).

Investigación agrícola dirigida por René Clara del CENTA: Desarrollo de nuevos cultivares.

Investigación agrícola y extensionismo es costoso:

Rentabilidad de las inversiones públicas?

Quiénes fueron los beneficiarios?

Returns To The Introduction Of New Sorghum Cultivars Into The Dairy Industry Of El Salvador, Alexis H. Villacís, John H. Sanders

INTSORMIL Presentations

The driver of agricultural development is the introduction of new technology. Sensitive sorghums have long been a critical component of hillside Central American agriculture providing a reserve or insurance policy when the principal staple of maize falters. The insensitive sorghums have been more recently introduced since the ‘70s for planting in the valleys in the second season. In the last thirty years the introduction of new insensitive cultivars has accelerated initially for poultry and in the previous two decades for dairy production. This growth has been facilitated by strategic investments in research by CENTA and the Ministry of Agriculture.

As …

Predator Mediated Selection And The Impact Of Developmental Stage On Viability In Wood Frog Tadpoles (Rana Sylvatica), Ryan Calsbeek, Shawn Kuchta

Dartmouth Scholarship

Complex life histories require adaptation of a single organism for multiple ecological niches. Transitions between life stages, however, may expose individuals to an increased risk of mortality, as the process of metamorphosis typically includes developmental stages that function relatively poorly in both the pre- and post-metamorphic habitat. We studied predator-mediated selection on tadpoles of the wood frog, Rana sylvatica, to identify this hypothesized period of differential predation risk and estimate its ontogenetic onset. We reared tadpoles in replicated mesocosms in the presence of the larval odonate Anax junius, a known tadpole predator.

Chronic Hindlimb Ischemia Impairs Functional Vasodilation And Vascular Reactivity In Mouse Feed Arteries, Trevor R. Cardinal, Kyle R. Struthers, Thomas J. Kesler, Matthew D. Yocum, David T. Kurjiaka, James B. Hoying

Peer Reviewed Articles

Vasodilation of lower leg arterioles is impaired in animal models of chronic peripheral ischemia. In addition to arterioles, feed arteries are a critical component of the vascular resistance network, accounting for as much as 50% of the pressure drop across the arterial circulation. Despite the critical importance of feed arteries in blood flow control, the impact of ischemia on feed artery vascular reactivity is unknown. At 14 days following unilateral resection of the femoral–saphenous artery–vein pair, functional vasodilation of the profunda femoris artery was severely impaired, 11 ± 9 versus 152 ± 22%. Although endothelial and smooth muscle-dependent vasodilation were …

Cooper, Thomas Poe, 1881-1958 (Sc 2488), Manuscripts & Folklife Archives

Manuscript Collection Finding Aids

Finding aid only for Manuscripts Small Collection 2488. Letter from Thomas Cooper, Dean and Director, Agricultural Extension Division, College of Agriculture and Home Economics, University of Kentucky, to Emma L. Barnes, Carlisle, Kentucky. He confirms her appointment to the University’s Agricultural Extension Division as an Assistant Home Demonstration Agent in Nicholas County at an annual salary of $2,700.

Statistical Optimization Of Blanching Time And Temperature Of Irish York Cabbage Using Desirability Function, Amit Jaiswal, Nissreen Abu-Ghannam, Shilpi Gupta

Articles

The effect of different heat treatments, as a means of preprocessing, on the phytochemicals present in Irish York cabbage was studied. A comparison of blanching (by immersing in water) and microwaving (using water as a medium) indicated that microwaving is detrimental to the phytochemicals present in cabbage. To achieve a blanching time and temperature combination that would result in minimal loss of phytochemicals, central composite design that integrates a desirability approach was used. A second-order polynomial equation was developed, indicating the effect of the blanching time and temperature on the total phenol content (TPC), total flavonoid content (TFC) and 2,2-diphenyl-1-picrylhydrazyl …

Global Change, Global Trade, And The Next Wave Of Plant Invasions, Bethany A. Bradley, Dana M. Blumenthal, Regan Early, Edwin D. Grosholz, Joshua J. Lawler, Luke P. Miller, Cascade J.B. Sorte, Carla M. D'Antonio, Jeffrey M. Diez, Jeffrey S. Dukes, Ines Ibanez, Julian D. Olden

Faculty Publications, Biological Sciences

Many non-native plants in the US have become problematic invaders of native and managed ecosystems, but a new generation of invasive species may be at our doorstep. Here, we review trends in the horticultural trade and invasion patterns of previously introduced species and show that novel species introductions from emerging horticultural trade partners are likely to rapidly increase invasion risk. At the same time, climate change and water restrictions are increasing demand for new types of species adapted to warm and dry environments. This confluence of forces could expose the US to a range of new invasive species, including many …

Management Zone Delineation Techniques On Irrigated Corn In Nebraska, Aaron M. Bereuter

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Increased interest in site-specific management of irrigated corn in Nebraska has identified the need for a decision-making framework for zone delineation. Zones based on static soil and landscape properties provide a valuable foundation for variable rate management strategies. The purpose of this project was to identify the optimal soil and landscape attributes for generating management zones (MZ) of relative productivity potential. Three producer-managed cornfields across Nebraska with varying soil types, topographic characteristics, and climate regimes were identified as research sites during the 2010 growing season. Nine soil and landscape attributes were identified as potential properties for estimation of productivity potential. …

A Study Of Correlations Between The Definition And Application Of The Gene Ontology, Yuji Mo

Department of Computer Electronics and Engineering: Dissertations, Theses, and Student Research

When using the Gene Ontology (GO), nucleotide and amino acid sequences are annotated by terms in a structured and controlled vocabulary organized into relational graphs. The usage of the vocabulary (GO terms) in the annotation of these sequences may diverge from the relations defined in the ontology. We measure the consistency of the use of GO terms by comparing GO's defined structure to the terms' application. To do this, we first use synthetic data with different characteristics to understand how these characteristics influence the correlation values determined by various similarity measures. Using these results as a baseline, we found that …

Pulmonary Diffusional Screening And The Scaling Laws Of Mammalian Metabolic Rates, Chen Hou, Michael Mayo

Biological Sciences Faculty Research & Creative Works

Theoretical considerations suggest that the mammalian metabolic rate is linearly proportional to the surface areas of mitochondria, capillary, and alveolar membranes. However, the scaling exponents of these surface areas to the mammals' body mass (approximately 0.9-1) are higher than exponents of the resting metabolic rate (RMR) to body mass (approximately 0.75), although similar to the one of exercise metabolic rate (EMR); the underlying physiological cause of this mismatch remains unclear. The analysis presented here shows that discrepancies between the scaling exponents of RMR and the relevant surface areas may originate from, at least for the system of alveolar membranes in …

Abiotic Stress Responses In Photosynthetic Organisms, Joseph Msanne

School of Natural Resources: Dissertations, Theses, and Student Research

Cellular and molecular aspects of abiotic stress responses in Arabidopsis thaliana subjected to cold, drought, and high salinity and in two photosynthetic green alga, Chlamydomonas reinhardtii and Coccomyxa sp. C-169, subjected to nitrogen deprivation were investigated. Cold, drought, and high salinity can negatively affect plant growth and crop production. The first research aimed at determining the physiological functions of the stress-responsive Arabidopsis thaliana RD29A and RD29B genes. Cold, drought, and salt induced both genes; the promoter of RD29Awas found to be more responsive to drought and cold stresses, whereas the promoter of RD29B was highly responsive to salt stress. …

Climate Change And Climate Variability: El Salvador – Impacts On Productivity Of Grain Crops And Opportunities For Management And Improvement, P. V. Vara Prasad

INTSORMIL Presentations

1. Climate change and climate variability (past and future).

2. Climate change and variability in El Salvador (climate models).

3. Impact of temperature on grain yield of dry bean, maize and sorghum in El Salvador (crop simulation models).

4. Effects of temperature, drought and/or carbon dioxide: experimental evidence (response of grain sorghum, maize and dry bean).

5. Opportunities for crop management and genetic improvement.

Maternal Smoking During Pregnancy And Risk Factors For Cardiovascular Disease In Adulthood, Bernardo Lessa Horta, Denise P. Gigante, Aydin Nazmi, Vera Maria F. Silveira, Isabel Oliveira, Cesar G. Victora

Food Science and Nutrition

Objective: This study was aimed at assessing the effect of maternal smoking during pregnancy on metabolic cardiovascular risk factors in early adulthood in a Brazilian birth cohort, after controlling for possible confounding variables and health behaviors in early adulthood.

Methods: In 1982, the maternity hospitals in Pelotas, southern Brazil, were visited and all births were identified. Those livebirths whose family lived in the urban area of the city were studied prospectively. In 2004–2005, we attempted to follow the whole cohort, the subjects were interviewed, examined and blood sample was collected. The following outcomes were studied: blood pressure; HDL cholesterol; triglycerides; …

How To Control Texture In Cultured Dairy, Phillip S. Tong

Dairy Science

No abstract provided.

Characterization Of The Tolerance Response In The Soybean Ks4202 To Aphis Glycines Matsumura, Travis J. Prochaska

Department of Entomology: Dissertations, Theses, and Student Research

Since the introduction of the soybean aphid, Aphis glycines Matsumura, to the soybean growing regions of the United States, the soybean aphid has caused considerable economic damage and yield loss to soybean growers. The objectives of this research were to evaluate selected genotypes for resistance to the soybean aphid and characterize transcriptional changes in response to aphid feeding to better understand the underlying tolerant mechanism(s) in KS4202 and genes contributing to its tolerance response. A field study (2009) was conducted to evaluate selected soybean genotypes during their reproductive stages for resistance to A. glycines. The economic injury level (EIL) …

Model Comparisons For Estimating Carbon Emissions From North American Wildland Fire, Nancy H. F. French, Willam J. De Groot, Liza K. Jenkins, Brendan M. Rogers, Ernesto Alvarado, Brian Amiro, Bernardus De Jong, Scott Goetz, Elizabeth Hoy, Edward Hyer, Robert Keane, B. E. Law, Donald Mckenzie, Steven G. Mcnulty, Roger Ottmar, Diego R. Perez-Salicrup, James Randerson, Kevin M. Robertson, Merritt Turetsky

Michigan Tech Research Institute Publications

Research activities focused on estimating the direct emissions of carbon from wildland fires across North America are reviewed as part of the North American Carbon Program disturbance synthesis. A comparison of methods to estimate the loss of carbon from the terrestrial biosphere to the atmosphere from wildland fires is presented. Published studies on emissions from recent and historic time periods and five specific cases are summarized, and new emissions estimates are made using contemporary methods for a set of specific fire events. Results from as many as six terrestrial models are compared. We find that methods generally produce similar results …

Emergent Behavior In A Coupled Economic And Coastline Model For Beach Nourishment, Eli D. Lazarus, D E. Mcnamara, M D. Smith, S Gopalakrishnan, A B. Murray

Publications

Developed coastal areas often exhibit a strong systemic coupling between shoreline dynamics and economic dynamics. "Beach nourishment", a common erosion-control practice, involves mechanically depositing sediment from outside the local littoral system onto an actively eroding shoreline to alter shoreline morphology. Natural sediment-transport processes quickly rework the newly engineered beach, causing further changes to the shoreline that in turn affect subsequent beach-nourishment decisions. To the limited extent that this landscape/economic coupling has been considered, evidence suggests that towns tend to employ spatially myopic economic strategies under which individual towns make isolated decisions that do not account for their neighbors. What happens …

Essential Oils Of Cupressus Funebris, Juniperus Communis, And J. Chinensis (Cupressaceae) As Repellents Against Ticks (Acari: Ixodidae) And Mosquitoes (Diptera: Culicidae) And As Toxicants Against Mosquitoes, John F. Carroll, Nurhayat Tabanca, Matthew H. Kramer, Natasha M. Elejalde, David E. Wedge, Ulrich R. Bernier, Monique Coy, James Becnel, Betul Demirci, Kemal Husnu Can Başer, Jian Zhang, Sui Zhang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Juniperus communis leaf oil, J. chinensis wood oil, and Cupressus funebris wood oil (Cupressaceae) from China were analyzed by gas chromatography and gas chromatography-mass spectrometry. We identified 104 compounds, representing 66.8-95.5% of the oils. The major components were: α-pinene (27.0%), α-terpinene (14.0%), and linalool (10.9%) for J. communis; cuparene (11.3%) and δ-cadinene (7.8%) for J. chinensis; and α-cedrene (16.9%), cedrol (7.6%), and β-cedrene (5.7%) for C. funebris. The essential oils of C. funebris, J. chinensis, and J. communis were evaluated for repellency against adult yellow fever mosquitoes, Aedes aegypti (L.), host-seeking nymphs of the lone …

Maternal Separation Affects Dopamine Transporter Function In The Spontaneously Hypertensive Rat: An In Vivo Electrochemical Study, Jacqueline S. Womersley, Jennifer H. Hsieh, Lauriston A. Kellaway, Greg A. Gerhardt, Vivienne A. Russell

Neuroscience Faculty Publications

BACKGROUND: Attention-deficit/hyperactivity disorder (ADHD) is a developmental disorder characterised by symptoms of inattention, impulsivity and hyperactivity. The spontaneously hypertensive rat (SHR) is a well-characterised model of this disorder and has been shown to exhibit dopamine dysregulation, one of the hypothesised causes of ADHD. Since stress experienced in the early stages of life can have long-lasting effects on behaviour, it was considered that early life stress may alter development of the dopaminergic system and thereby contribute to the behavioural characteristics of SHR. It was hypothesized that maternal separation would alter dopamine regulation by the transporter (DAT) in ways that distinguish SHR …

Utilizing Fishermen Knowledge And Expertise: Keys To Success For Collaborative Fisheries Research, Noëlle Yochum, Richard M. Starr, Dean Wendt

Biological Sciences

Collaborative fisheries research provides a mechanism for integrating the unique knowledge, experience, and skills of fishermen and scientists. It is a joint intellectual endeavor that begins with the inception of a project and continues until its final stages, with each group having mutual investment in—and ownership of— the project. Collaborative fisheries research promotes communication and trust among fishermen, scientists, and managers and can provide much-needed scientifically valid data for fisheries management. It can enhance federal and state management data collection programs, which span broad sections of coastline, by increasing the ability to detect changes in local metapopulations that may be …

What Are The Effects Of Raw Vs. Pasteurized Milk Consumption On Growth Rate And Fertility In A Colony Of Mice?, Kari Sholing, Lindsay Ganong, Amy Olson

Nutrition Student Work

Interest in consuming unprocessed foods has led to assumptions that raw milk has health benefits over pasteurized milk. This study was designed to evaluate the belief that raw milk is nutritionally superior. Eighteen mice were randomly assigned to one of two groups: raw milk or pasteurized milk. Mice were grouped into breeding trios and given a fresh supply of milk every four hours between 7 a.m. and 11 p.m. for 55 days. Milk consumption was tracked at each feeding by measuring the amount of milk that was provided and the amount of milk that was left from the previous feeding. …

Heritable Choice Of Colony Size In Cliff Swallows: Does Experience Trump Genetics In Older Birds?, Erin A. Roche, Charles R. Brown, Mary Bomberger Brown

School of Natural Resources: Faculty Publications

The variation in breeding colony size seen in populations of most colonial birds may reflect heritable choices made by individuals that are phenotypically specialized for particular social environments. Although a few studies have reported evidence for genetically based choice of group sizes in birds, we know relatively little about the extent to which animals potentially rely on experience versus innate preferences in deciding how many conspecifics to settle with at different times of their lives. We conducted a cross-fostering experiment in 1997–1998 on cliff swallows, Petrochelidon pyrrhonota, in southwestern Nebraska, USA, in which some individuals were reared in colonies …

Scotts Bluff National Monument Plant Community Composition And Structure Monitoring, 2011 Annual Report, Isabel W. Ashton, Michael Prowatzke, Michael R. Bynum, Tim Shepherd, Stephen K. Wilson, Kara Paintner-Green

United States National Park Service: Publications

Executive Summary

The Northern Great Plains Inventory & Monitoring Network (NGPN) was established to develop and provide scientifically credible information on the current status and long-term trends of the composition, structure, and function of ecosystems in thirteen parks located in five northern Great Plains states. NGPN identified upland plant communities, exotic plant early detection, and riparian lowland communities as vital signs that can be used to better understand the condition of terrestrial park ecosystems (Gitzen et al. 2010). Upland and riparian ecosystems are important targets for vegetation monitoring because the status and trends in plant communities provide critical insights into …

Agate Fossil Beds National Monument Plant Community Composition And Structure Monitoring, 2011 Annual Report, Isabel W. Ashton, Michael Prowatzke, Michael R. Bynum, Tim Shepherd, Stephen K. Wilson, Kara Paintner-Green

United States National Park Service: Publications

Executive Summary

The Northern Great Plains Inventory & Monitoring Network (NGPN) was established to develop and provide scientifically credible information on the current status and long-term trends of the composition, structure, and function of ecosystems in thirteen parks located in five northern Great Plains states. NGPN identified upland plant communities, exotic plant early detection, and riparian lowland communities as vital signs that can be used to better understand the condition of terrestrial park ecosystems (Gitzen et al. 2010). Upland and riparian ecosystems are important targets for vegetation monitoring because the status and trends in plant communities provide critical insights into …

Hands-On Labs (Hol): Using Technology And Web-Based Resources To Transform A Traditional Lab-Based Biology Course Into A Distance Learning (Dl) Hybrid Course, Across Schools At Pace University., Erica Kipp

Cornerstone 3 Reports : Interdisciplinary Informatics

No abstract provided.

Animal Pain: What It Is And Why It Matters, Bernard E. Rollin

Animal Welfare Collection

The basis of having a direct moral obligation to an entity is that what we do to that entity matters to it. The ability to experience pain is a sufficient condition for a being to be morally considerable. But the ability to feel pain is not a necessary condition for moral considerability. Organisms could have possibly evolved so as to be motivated to flee danger or injury or to eat or drink not by pain, but by ‘‘pangs of pleasure’’ that increase as one fills the relevant need or escapes the harm. In such a world, ‘‘mattering’’ would be positive, …