Life Sciences Commons^™

Hematopoiesis Of Immature Myeloid Dendritic Cells In Stroma-Dependent Spleen Long-Term Cultures Occurs Independently Of Nf-Kb/Relb Function, Jonathan Tan, Keping Ni, Fei Le, Helen O'Neill

Jonathan Tan

Objective The nuclear factor-κB (NF-KB)/RelB transcription factor plays an essential role in development of some dendritic cell (DC) subsets in mice. In this laboratory, immature myeloid DC are produced in vitro in a stroma-dependent murine spleen long-term culture (LTC) system. In LTC, DC differentiate from hematopoietic progenitors maintained within the stromal cell matrix. Expression and function of RelB in development of LTC-DC has been investigated, with a view to assessing the relationship between DC produced in this system and other known subsets of DC. Materials and Methods RelB expression by LTC-DC was confirmed by detection of protein by Western blotting, …

Concise Review: Dendritic Cell Development In The Context Of The Spleen Microenvironment, Jonathan Tan, Helen O'Neill

Jonathan Tan

The dendritic cell (DC) population in spleen comprises a mixture of cells including endogenous DC progenitors, DC precursors migrating in from blood and bone marrow, and DC in different states of differentiation and activation. A role for different microenvironments in supporting the dynamic development of murine DC of different types or lineages is considered here. Recent evidence for production of DC dependent on splenic stromal cells is reviewed in the light of evidence that cell production is dependent on cells comprising an endothelial niche in spleen. The possibility that self-renewing progenitors in spleen give rise to DC with tolerogenic or …

Pioneering Lobster Aquaculture In Rhode Island, Michael A. Rice

Michael A Rice

Analyzing In Situ Gene Expression In The Mouse Brain With Image Registration, Feature Extraction And Block Clustering, Manjunatha Jagalur, Chris Pal, Erik G. Learned-Miller, R R Thomas Zoeller, David Kulp

Erik G Learned-Miller

Background: Many important high throughput projects use in situ hybridization and may require the analysis of images of spatial cross sections of organisms taken with cellular level resolution. Projects creating gene expression atlases at unprecedented scales for the embryonic fruit fly as well as the embryonic and adult mouse already involve the analysis of hundreds of thousands of high resolution experimental images mapping mRNA expression patterns. Challenges include accurate registration of highly deformed tissues, associating cells with known anatomical regions, and identifying groups of genes whose expression is coordinately regulated with respect to both concentration and spatial location. Solutions to …

Microarray-Based Method For Detection Of Unknown Genetic Modifications, Torstein Tengs

Dr. Torstein Tengs

Background

Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed a strategy for detection and characterization of unknown genetic modifications and we present a proof of concept for this method using Arabidopsis thaliana and Oryza sativa (rice). The approach relies on direct hybridization of total genomic DNA to high density microarrays designed to have probes tiled throughout a set of reference sequences.

Results

We show that by using arrays with 25 basepair probes covering both …

Vector And Parameters For Targeted Transgenic Rna Interference In Drosophila Melanogaster, Jian Quan Ni, Michele Markstein, Richard Binari, Barret Pfeiffer, Lu Ping Liu, Christians Villalta, Matthew Booker, Lizabeth Perkins, Norbert Perrimon

Michele Markstein

The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs have been inserted into the genome randomly using P-element–mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.

Ecological Services To And From Rangelands Of The United States, Ed Frederickson, Kris Havstad, Debra Peters, Rhonda Skaggs, Joel Brown, Brandon Bestelmeyer, Jeffrey Herrick, Jack Wright

Ed L. Frederickson

The over 300 million ha of public and private rangelands in the United States are characterized by low and variable precipitation, nutrient-poor soils, and high spatial and temporal variability in plant production. This land type has provided a variety of goods and services, with the provisioning of food and fiber dominating through much of the 20th century. More recently, food production from a rangeland-based livestock industry is often pressured for a variety of reasons, including poor economic returns, increased regulations, an aging rural population, and increasingly diverse interests of land owners. A shift to other provisioning, regulating, cultural, and supporting …

Never Mind Grendel! Can Beowulf Conquer The 21st-Century Guilt Trip?, Stephen Asma

Stephen T Asma

The writer casts doubt on whether the emasculated Beowulf put forward by J. R. R. Tolkien and in the recent movie version of the story transcends and nullifies the heroic original. He suggests that both Beowulfs may be necessary.

Fate And Transport Of Pathogen Indicators From Pasturelands, Michelle L. Soupir

Michelle L. Soupir

The U.S. EPA has identified pathogen indicators as a leading cause of impairments in rivers and streams in the U.S. Elevated levels of bacteria in streams draining the agricultural watersheds cause concern because they indicate the potential presence of pathogenic organisms. Limited understanding of how bacteria survive in the environment and are released from fecal matter and transported along overland flow pathways results in high uncertainty in the design and selection of appropriate best management practices (BMPs) and in the bacterial fate and transport models used to identify sources of pathogens.

The overall goal of this study was to improve …

Laminar Flow In Mini-Fluidics Channels Assembly And Its Application In Zebra Fish Embryo Research, Radek Glaser

Radek Glaser

A Mini-Fluidics system was designed to facilitate the muscle growth of the Zebra Fish embryos. This experimental device is made of peristaltic pump, inflow/outflow manifolds, fluid storage tank, series of valves and flexible pipes and the main plate with six mini channels. These closed channels provide pathways for an extremely laminar flow. The Zebra Fish embryos are placed in the channels and exposed to the forces present in the fluid.

Reduced Soil Compaction Enhances Establishment Of Non-Native Plant Species, G. Page Kyle, Karen H. Beard, Andrew Kulmatiski

Karen H. Beard

Many studies have shown that soil disturbance facilitates establishment of invasive, non-native plant species, and a number of mechanisms have been isolated that contribute to the process. To our knowledge no studies have isolated the role of altered soil compaction, a likely correlate of many types of soil disturbance, in facilitating invasion. To address this, we measured the response of seeded non-native and native plant species to four levels of soil compaction in mesocosms placed in an abandoned agricultural field in the Methow Valley, Washington, USA. Soil compaction levels reflected the range of resistance to penetration (0.1-3.0 kg cm(-2)) measured …

Statistical Issues In Proteomic Research, Jeffrey S. Morris

Jeffrey S. Morris

No abstract provided.

Systemic And Mucosal Infection Program Protective Memory Cd8 T Cells In The Vaginal Mucosa., Thandi M. Onami, P. K. Suvas, H. M. Dech, J. Zeng

Thandi M. Onami

Whether mucosal immunization is required for optimal protective CD8 T cell memory at mucosal surfaces is controversial. In this study, using an adoptive transfer system, we compare the efficacy of two routes of acute lymphocytic choriomeningitis viral infection on the generation, maintenance, and localization of Ag-specific CD8 T cells in tissues, including the vaginal mucosa. Surprisingly, at day 8, i.p. infection results in higher numbers of Ag-specific CD8 T cells in the vaginal mucosa and iliac lymph node, as well as 2-3x more Ag-specific CD8 T cells that coexpress both IFN-gamma and TNF-alpha in comparison to the intranasal route of …

Agris: An Fao Information Resource For The Agricultural Journalist, Joe Zumalt

Joseph R. Zumalt

A review of AGRIS.

Non-Standard Sources In A Standardized World: Responsible Practice And Ethics Of Acquiring Turtle Specimens For Scientific Use, Edgar Lehr, Russell Burke, Linda Ford, Steve Mockford, Peter Pritchard, Jose Rosado, Darrell Senneke, Bryan Stuart

Edgar Lehr

No abstract provided.

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Serg/Gorduj Gordienko

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Polybrominated Diphenyl Ethers And Organochlorine Pesticides In Human Milk From Massachusetts, Usa, Kathleen Arcaro, B. Johnson-Restrepo, R. Addink, C. Wong, K. Kannan

Kathleen Arcaro

Concentrations of polybrominated diphenyl ethers (PBDEs), and organochlorine pesticides (OCPs; DDTs, HCHs, CHLs, and HCB) were measured in human breast milk samples collected across Massachusetts, USA, in 2004. Seventeen PBDE congeners were found in the samples, ranging in concentration from 0.06 to 1910 ng g(-1) lipid wt. BDE-47 (2,2',4,4'-tetraBDE), BDE-99 (2,2',4,4',5-pentaBDE), and BDE-100 (2,2',4,4',6-pentaBDE) were the major congeners detected in breast milk samples. Overall mean (+/-SD) concentrations of DDTs, HCHs, CHLs, and HCB were 64.5 +/- 75, 18.9 +/- 19, 32.4 +/- 36, and 2.3 +/- 2.2 ng g(-1) lipid wt, respectively. Concentrations of PBDEs were strongly correlated with concentrations …

Growth Of Thermophilic And Hyperthermophilic Fe(Iii)-Reducing Microorganisms On A Ferruginous Smectite As The Sole Electron Acceptor, Derek Lovley, Kazem Kashefi, Evgenya S. Shelobolina, W. Crawford Elliott

Derek Lovley

Recent studies have suggested that the structural Fe(III) within phyllosilicate minerals, including smectite and illite, is an important electron acceptor for Fe(III)-reducing microorganisms in sedimentary environments at moderate temperatures. The reduction of structural Fe(III) by thermophiles, however, has not previously been described. A wide range of thermophilic and hyperthermophilic Archaea and Bacteria from marine and freshwater environments that are known to reduce poorly crystalline Fe(III) oxides were tested for their ability to reduce structural (octahedrally coordinated) Fe(III) in smectite (SWa-1) as the sole electron acceptor. Two out of the 10 organisms tested, Geoglobus ahangari and Geothermobacterium ferrireducens, were not able …

Spatial And Temporal Patterns Of Nitrogen Deposition In China: Synthesis Of Observational Data, Chaoqun (Crystal) Lu, Hanqin Tian

Chaoqun (Crystal) Lu

Anthropogenic nitrous pollutant emissions in China significantly increased during the last decades, which contributed to the accelerated nitrogen (N) deposition. In order to characterize spatial pattern of nitrogen deposition, we employed the kriging technique to interpolate sampling data of precipitation chemistry and ambient air concentration from site-network observations over China. The estimation of wet deposition in China was limited to aqueous NO3− and NH4+, while ambient NO2 was the only species involved in the predicted dry deposition fluxes. To obtain wet deposition fluxes, precipitation concentration was multiplied by 20-year mean precipitation amounts with a resolution of 10 × 10 km. …

Long-Term Tillage And Cropping Sequence Effects On Dryland Residue And Soil Carbon Fractions, Upendra M. Sainju, Thecan Caesar-Tonthat, Andrew W. Lenssen, Robert G. Evans, Robert Kolberg

Andrew W. Lenssen

Long-term management practices are needed to increase dryland C storage and improve soil quality. We evaluated the 21-yr effects of combinations of tillage and cropping sequences on dryland crop biomass (stems + leaves) returned to the soil, residue C, and soil C fractions at the 0- to 20-cm depth in a Dooley sandy loam (fine-loamy, mixed, frigid, Typic Argiborolls) in eastern Montana. Treatments were no-till continuous spring wheat (Triticum aestivum L.) (NTCW), spring-tilled continuous spring wheat (STCW), fall- and spring-tilled continuous spring wheat (FSTCW), fall- and spring-tilled spring wheat–barley (Hordeum vulgare L.) (1984–1999) followed by spring wheat–pea (Pisum sativum L.) …

Dynamic Water Adsorption Characteristics Of Distillers Dried Grains With Solubles (Ddgs), V. Ganesan, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

Distillers dried grains with solubles (DDGS) is one of the coproducts obtained from dry-grind ethanol manufacturing. As the ethanol industry is growing exponentially, the production of DDGS has been significantly increasing as well. To optimize the use of DDGS, it has to be economically transported from one part of the country to other parts, and stored efficiently. But DDGS has some flow issues, which often makes storage and transportation very problematic. So the objective of this study was to investigate the dynamic water adsorption characteristics of DDGS with four soluble levels at four temperatures and four relative humidities. Three mathematical …

Producer-Scrounger Roles And Joining Based On Dominance In A Free-Living Group Of Mexican Jays (Aphelocoma Ultramarina), John Mccormack, Piotr Jablonski, Jerram Brown

John E. McCormack

While foraging, animals often exploit group members to obtain food. One way to describe this behaviour is with the producer-scrounger (PS) model, where scroungers use social interaction to obtain food discovered by producers. Mexican jays (Aphelocoma ultramarina) are a groupforaging species with a linear dominance hierarchy. We studied interactions in a free-living foraging group to determine (1) if foraging interactions can be explained with the PS model, (2) if these roles are consistent and (3) if dominance or relatedness affects joining frequency. We recorded board-flipping, eating, and joining events during sets of feeding trials. We show that Mexican jays use …

Effects Of Eugenol, Α-Terpineol, Terpin-4-Ol, And Methyl Eugenol On Consumption Of Alfalfa Pellets By Sheep, Ed Frederickson

Ed L. Frederickson

Many secondary compounds are typically present in unpalatable shrubs on arid and semi-arid rangelands. However, the relationship between intake by livestock and concentration of individual chemicals has been examined for very few of these compounds. Four experiments were conducted to examine effects of individual volatile compounds on intake of alfalfa pellets by lambs. Forty-five lambs (9 lambs/treatment) were individually fed alfalfa pellets with eugenol, α-terpineol, terpin-4-ol, or methyl eugenol applied at one of five concentrations. Treatments were multiples (0, 0.5, 1, 2, and 10×) of the concentration (×) of each compound on the leaf surface of Flourensia cernua. Treatment solutions …

Recent Postglacial Range Expansion Drives The Rapid Diversification Of A Songbird Lineage In The Genus Junco, Borja Milá, John Mccormack, Gabriela Castañeda, Robert Wayne, Thomas Smith

John E. McCormack

Pleistocene glacial cycles are thought to have played a major role in the diversification of temperate and boreal species of North American birds. Given that coalescence times between sister taxa typically range from 0.1 to 2.0 Myr, it has been assumed that diversification occurred as populations were isolated in refugia over long periods of time, probably spanning one to several full glacial cycles. In contrast, the rapid postglacial range expansions and recolonization of northern latitudes following glacial maxima have received less attention as potential promoters of speciation. Here we report a case of extremely rapid diversification in the songbird genus …

Influence Of The Hydrodynamic Environment On Quorum Sensing In Pseudomonas Aeruginosa Biofilms, Jeffrey J. Margolis, Mary Jo Kirisits, Boloroo L. Purevdorj-Gage, Benjamin Vaughan, David L. Chopp, Paul Stoodley, Matthew R. Parsek

Jeffrey Margolis

Cerebrospinal Fluid And Serum Biomarkers Of Cerebral Malaria Mortality In Ghanaian Children, Winston A. Anderson

Winston Anderson

Biogeographical Distribution And Natural Groupings Among Five Sympatric Wild Cats In Tropical South Asia, Mohammed Ashraf

Mohammed Ashraf

Anopheles Gambiae Purine Nucleoside Phosphorylase: Catalysis, Structure And Inhibition, Erika Taylor, Agnes Rinaldo-Matthis, Lei Li, Mahmoud Ghanem, Keith Hazleton, M. Belen Cassera, Steven Almo, Vern Schramm

Erika A. Taylor, Ph.D.

The purine salvage pathway of Anopheles gambiae, a mosquito that transmits malaria, has been identified in genome searches on the basis of sequence homology with characterized enzymes. Purine nucleoside phosphorylase (PNP) is a target for the development of therapeutic agents in humans and purine auxotrophs, including malarial parasites. The PNP from Anopheles gambiae (AgPNP) was expressed in Escherichia coli and compared to the PNPs from Homo sapiens (HsPNP) and Plasmodium falciparum (PfPNP). AgPNP has kcat values of 54 and 41 s-1 for 2'-deoxyinosine and inosine, its preferred substrates, and 1.0 s-1 for guanosine. However, the chemical step is fast for …

The Potential Role Of Probiotics In Reducing Poverty-Associated Infections In Developing Countries, Kingsley C. Anukam

Kingsley C Anukam

Probiotics are defined by the Food and Agricultural Organization/ World Health Organization as “live microorganisms which when administered in adequate amounts confer a health benefit on the host” [1]. The potential benefits of their use have not been adequately investigated, especially in the developing world. Japan introduced Yakult, a probiotic fermented food drink in 1935, and in the Northern hemisphere, research and use of probiotics has gained an unprecedented momentum in the last decade [2]. Use of probiotics is not uncommon in Europe [3], but in many developing countries use of probiotics in its present definition is a foreign concept. …

Looking Up From The Gutter: Pop-Culture And Philosophy, Stephen Asma

Stephen T Asma

No abstract provided.