Life Sciences Commons^™

Successful Weight Management: Barriers And Facilitators To Maintaining Weight After Weight Loss Via A Meal Replacement Program, Hope D. Kleine

Electronic Theses and Dissertations

Purpose: Meal replacement programs (MRPs) facilitate weight loss. Unfortunately, a large percentage of individuals that lose weight through MRP are not successful at maintaining their weight. Weight regain one year following weight loss via meal replacements has been as high as 40-50%, demonstrating a critical need to improve weight maintenance in MRP participants. Therefore, the purpose of this study is to identify barriers and facilitators of weight maintenance after reaching a goal weight in MRP participants.

Methods: Seven focus groups of ≤8 clients were conducted to discuss barriers and facilitators of weight maintenance prior to reaching a point of …

Icook 4-H: Report Of Accelerometer Derived Physical Activity In 9-10 Year Old Children From Baseline, Post, And Follow-Up, Chase Merfeld

Electronic Theses and Dissertations

To examine the relationship between baseline, post, and follow-up accelerometer derived physical activity (PA) in 9-10 year old children participating in iCook 4-H, a 16-week, 5- state, randomized control family centered childhood obesity prevention program. This family focus intervention was designed to promote PA through lifestyle modifications. Between group differences in minutes-per-day and intensity of PA were determined on accelerometer compliant participants. All subjects wore an accelerometer (Actigraph GT3X+) for 7 days at baseline, post, and follow-up intervention assessment. Mean daily minutes of accelerometer derived sedentary time (ST) and PA including light (LPA), moderate (MPA), vigorous (VPA) and moderate-to-vigorous (MVPA) …

Fish Ladders Designed For Drop Culverts And Central Stoneroller Ecology Across A Latitudinal Gradient, John Arthur Lorenzen

Electronic Theses and Dissertations

Stream connectivity is essential for the persistence of fish populations and maintenance of fish assemblage structure. Stream-road crossings can become barriers resulting in a loss of connectivity. Creating innovative tools for fisheries managers to allow fish passage through barrier stream-road crossings is important worldwide as climate change may force range shifts to stay within thermal requirements. Understanding how populations and metapopulations respond to climate is important as fisheries managers can use this information to make informed management decisions in regards to climate change and possible range shifts. Thus, we developed and tested a fish ladder to retrofit barrier stream-road crossings …

Characterization Of Small Non-Coding Rnas In The Seminal Plasma Of Beef Bulls With Predicted High And Low Fertility, Stephanie Perkins

Electronic Theses and Dissertations

MicroRNAs (miRNAs) are a family of small non-coding RNAs (sRNA) that play a key role in regulating gene expression by binding to their complementary mRNA and altering translation. It has been reported that this down-regulation of specific areas of the genome plays a role in male fertility. Piwi-interacting RNAs (piRNAs) are another class of germline-specific non-coding RNAs that form silencing complexes to maintain germline genome integrity. In bovine seminal plasma, piRNAs have not previously been characterized. Thus, the objective of this study was to investigate a potential relationship between expression of these sRNAs and fertility in beef bulls and to …

Use Of Extraction Technologies In Food Safety Studies, Changling Qiu

Electronic Theses and Dissertations

Food safety has become a top concern in our society. The public in general is increasingly concerned about the safety of the food products they consume every day as more and more food contamination incidents and widespread recalls arise. It is necessary to trace any presence and/or the concentration levels of contaminants, pesticides, herbicides, or other harmful substances in food samples. Sample preparation is a crucial step in a food analytical method, as it takes up most of the total analysis time, contributing highly to the total cost of analysis and greatly influencing the results of the analysis. Traditional extraction …

The Behavioral, Biochemical And Genetic Effects Of Sleep Deprivation In Zebrafish (Danio Rerio), Jade Catherine Boykin

Electronic Theses and Dissertations

Vertebrate sleep is a universal phenomenon and encompasses an array of conserved behavioral and physiological characteristics. Studies of sleep in humans have shown that sleep deprivation leads to adverse effects to human health and additional stress. Teleosts have emerged as a useful model species to study the vertebrate stress response, given its stress axis has high levels of homology with the mammals, including humans. For this study, the behavior of zebrafish (Danio rerio) subjected to acute (24 hours) and chronic (20 days) extended light exposure was monitored and whole-body cortisol was analyzed as an indication of activation of …

A Phenomenological Investigation Of Cheerleaders' Lived Experiences Of Mental Blocks, Erin M. Lawrence

Electronic Theses and Dissertations

A mental block is a phenomenon in which an athlete’s mind no longer allows them to perform a physical skill that was previously well-learned and automatic. This phenomenon has received attention in gymnastics and trampoline but the literature is lacking an investigation of this phenomenon in all-star cheerleading. The purpose of this study was to examine the phenomenon of mental blocks by obtaining it from the first-person perspective of cheerleaders themselves. A purposeful sample of 8 all-star cheerleader participants was interviewed and transcriptions were analyzed utilizing qualitative analysis. Four main themes were found: 1) specific to backwards moving skills, 2) …

Small Molecule Disruption Of Ros-Regulating Pathways In Human Carcinoma, Rebecca E. Mccall

Electronic Theses and Dissertations

Gold (I) coordinated N-heterocyclic carbenes containing appended redox cycling moieties were designed and analyzed for their ability to target the antioxidant network via multiple mechanisms in various human cancer cell models. Complexes containing either ferrocene or naphthoquinone redox cycling moieties were screened for inhibition of cell growth, inhibition of thioredoxin reductase (TrxR), and accentuation of exogenous ROS. The cell death pathway employed was determined by flow cytometry via detection of Annexin-V FITC. Furthermore, differential gene expression between treated cells and untreated cells revealed the induction of endoplasmic reticulum stress and unfolded protein response pathways.

Assemblage Structure, Production, And Food Web Dynamics Of Macroinvertebrates In Tropical Island Headwater Streams, Keysa G. Rosas-Rodriguez

Electronic Theses and Dissertations

Variation in long-term temperature and precipitation patterns will likely influence the decomposition and export of benthic organic matter and influence aquatic macroinvertebrate consumer communities. Tropical systems are relatively understudied; therefore basal information is urgently needed. As part of an ongoing long-term study, we monitored macroinvertebrates in two shrimp-dominated and fishless headwater streams within the Luquillo Experimental Forest in Puerto Rico from 2009-2010. We combined growth rates with yearly biomass data to calculate secondary production and examined gut contents to develop quantitative food webs. Macroinvertebrate assemblages were dominated by a few insect taxa, with similar biotic composition across streams and habitats, …

Female Natural Bodybuilding Competition Preparation: A 6-Week Case Study, Samantha G. Gwazdauskas

Electronic Theses and Dissertations

Bodybuilding is unlike any other sport. Competitors are judged on appearance rather than performance, where muscle size and definition are keys to achieving success. This case study tracked four, drug-free, female bodybuilders for 6-weeks leading up to their competitions. The purpose of this project was to examine the physiological and psychological experiences of female bodybuilders as they prepare for competition. Each competitor reported for testing at three occasions to evaluate cardiovascular measures, body composition, resting metabolic rate, performance measures of strength, power, muscular endurance, and maximal oxygen consumption, mood state, social physique anxiety, and overall experience. Each subject in this …

Abiotic And Biotic Factors Influence Refuge Use At The Community And Organismal Level, George Todd

Electronic Theses and Dissertations

Animal behavior is influence by a wide range of factors. One factor that can heavily influence behavior is the presence or absence of refuge (i.e., refuge provides a direct benefit to animals’ fitness). Many animals seek refuge to avoid predation despite clear costs to other life processes. The decision to use refuge is complex and anthropogenic activities may alter the abundance of refuge. Artificial refuge structures can be successful in conservation efforts and are an effective means to measure biodiversity. Thus, I used cover boards to examine how habitat structure and season influence vertebrate abundance and diversity in the southeastern …

Assessing Shoreline Change And Vegetation Cover Adjacent To Back-Barrier Shoreline Stabilization Structures In Georgia Estuaries, Katherine R. Wakefield

Electronic Theses and Dissertations

Anthropogenic stabilization of erosional shorelines by hard-armoring structures is used for protection of property, especially if buildings, historical monuments, cultural resources, or other infrastructure are present. The post-installation effects of shoreline stabilization structures on adjacent shorelines in the back-barrier marshes of coastal Georgia are a concern, and interest in living shorelines as erosion control devices has increased because of their use of natural materials and vegetation. AMBUR shoreline analysis software was used to calculate pre-and post-installation shoreline change rates of shorelines adjacent to riprap and bulkhead structures. There was no significant difference between the post-installation shoreline change rates of the …

Combined Effects Of Tetrakis Hydroxymethyl Phosphonium Chloride And Ammonium On Plankton Community Structure In Blackwater Pond Mesocosms, Marina N. Osier

Electronic Theses and Dissertations

Flame retardant chemicals enter aquatic systems through municipal and industrial wastewater, and despite being detected in surface waters, most have not been thoroughly assessed for environmental risk. Halogenated flame retardants, known to be persistent and highly toxic to aquatic organisms are being phased out, and replaced with new or under-tested organophosphorus flame retardants. Organophosphorus flame retardants, such as tetrakis hydroxymethyl phosphonium chloride (THPC), often have similar applications to and are generally not as persistent as halogenated flame retardants. Single-species toxicity tests of organophosphorus flame retardants reveal a wide range of toxicity values within the range of environmentally relevant concentrations in …

Biochemical Composition Of Caribbean Gorgonian Corals And Their Symbiosis Under Ambeint And Stressful Conditions, Kartick Prakash Shirur

Electronic Theses and Dissertations

Gorgonian corals are the dominant benthic fauna on Caribbean reefs. Unlike scleractinian corals, gorgonian abundance on these reefs has remained stable or has increased. Therefore, to predict how climate change will affect the reef community, it is essential to understand gorgonian physiology under ambient and stressful situations. Under ambient conditions, eight gorgonian species hosted different clade B Symbiodinium, and in two gorgonian species in which the bacterial microbiomes were investigated, two distinct communities were found. In the eight gorgonian species, skeletal content varied from 75-96%, with those having more tissue also having more Symbiodinium density within their branches. The composition …

Genetic And Temporal Variation In Resource Exchange Between An Ectomycorrhizal Fungus, Rhizopogon Occidentalis, And Seedlings Of Pinus Radiata, Mariah Meachum

Electronic Theses and Dissertations

Various economic theories have been adapted to explain the evolutionary persistence of resource exchange mutualisms such as mycorrhizae. The ratio of resources exchanged is an important variable comto all these models. Measuring resource exchange ratios is the first step in testing the hypotheses predicted by these models and will provide insight into resource dynamics of mutualisms. We examined how the carbon, nitrogen and phosphorus resource exchange dynamics between Pinus radiata and an ectomycorrhizal fungus, Rhizopogon occidentalis, varied between native populations of Pinus radiata and over the first 64 weeks of the mycorrhizal mutualism. Using a mycocosm approach, the C:N and …

Spine Lifting Biomechanics Between Varying Occupational Activity Levels And Recreational Physical Activity Levels, Caitlin Rose Francis

Electronic Theses and Dissertations

Background: Moderate to vigorous physical activity as the optimum movement patterns for health have continued to be the dominant focus of health and fitness research. Yet, emerging evidence of deleterious, adverse health effects of prolonged inactivity, independent of regular physical activity, presents a new element to establishing the ideal model of movement patterns for health. The musculoskeletal trunk of the body becomes unbalanced as a result of prolonged inactivity, and a biomechanical analysis can help to identify high-risk loading behavior associated with these unbalances. Moreover, poor spine biomechanics can indicate a need for adjustment to present recommendations for optimum movement …

The Effect Of Pitching With Underweight And Overweight Balls On Pitch Velocity In Collegiate Baseball Pitchers., Hillary Ake

Electronic Theses and Dissertations

BACKGROUND: In an effort to improve overhand throw velocity in baseball pitchers, weighted implement training, which utilizes balls that are heavier or lighter than a competition ball, have been employed. Weighted ball programs have previously been used in baseball pitchers ranging from high school to professional with varying ball weights with mixed results (Straub, 1966; Brose and Hanson, 1967; DeRenne, 1985; DeRenne, 1990; van den Tillaar and Ettema, 2011). PURPOSE: To determine the effect of a commercially available weighted ball program on the throwing velocity of collegiate baseball pitchers over the course of an off-season. METHODS: This retrospective study examined …

Assessment Of Anti-Saccades Within 24 To 48 Hours Post-Concussion, Nathan D'Amico

Electronic Theses and Dissertations

INTRODUCTION: Oculomotor control dysfunction is present in about 90% of concussed athletes, with anti-saccades being the most prominent. PURPOSE: To investigate anti-saccades, reflexive gaze deviations from a fixed point or area of interest, between NCAA Division I athletes 24 to 48 hours post-concussion (PC) and healthy, matched controls (MC). METHODS: 10 PC (4 female, 6 male; age: 18.9 ± 0.9 years) and 10 MC (4 female, 6 male; age: 18.3 ± 0.6 years) wore a monocular eye tracker (240Hz) while performing 2 trials of the 60-second WiiFit Soccer Heading game. During play, participants were instructed to not deviate their gaze …

The Nutritional Value Of Fecal Sacs: Testing The Parental Nutrition Hypothesis, Amanda W. Burns

Electronic Theses and Dissertations

Nest sanitation is an important aspect of parental investment in birds with altricial nestlings. Passerine nestlings produce fecal matter that is encased in a mucous membrane for easy disposal. These fecal sacs are either removed from the nest and transported a distance away or they are ingested by the adult. A decline in fecal sac ingestion rates as nestlings age has been observed in several species. The parental nutrition hypothesis posits that this pattern can be attributed to increasing digestive efficiency as nestlings age. The parental nutrition hypothesis makes three predictions: fecal sac consumption rates should decrease as nestlings age, …

Effects Of Beta-Alanine Supplementation And High Intensity Interval Training Among Recreationally Active Females, Mary E. Yarbrough

Electronic Theses and Dissertations

Beta-alanine is a non-essential amino acid that when combined with L-histidine forms the dipeptide carnosine. Recent research has shown that supplementary intake of beta-alanine can substantially increase carnosine content in muscle fibers and has been associated with attenuating fatigue and enhancing high intensity exercise performance. The aim of this study was to examine the effects of 4 weeks of beta-alanine supplementation combined with high intensity interval training (HIIT) on indices of aerobic and anaerobic performance, rowing performance, and body composition. Twenty-one recreationally active females (22.2 ± 2.2 yrs.) participated in a double blind, placebo controlled study and were randomly assigned …

The Effects Of Thermal Opportunity And Habitat On Physiological Performance In Ecologically Distinct Populations Of The Florida Scrub Lizard Sceloporus Woodi, Lauren K. Neel

Electronic Theses and Dissertations

An ectotherm’s ability to thermoregulate affects many physiological traits. Therefore, thermal factors are paramount when considering behavior, activity time, body temperatures, energy budget, and performance capabilities. The Florida scrub lizard (Sceloporus woodi) is a ground-dwelling lizard that occupies longleaf pine and sand pine scrub habitats in the Ocala National Forest. Here, this species’ habitat is maintained by either clear-cut logging in scrub stands, or prescribed burning in long leaf pine stands. These habitat types differ in their dominant vegetation, canopy cover, and availability of vertical perches and thus are ecologically different. I used biophysical models to measure the …

Effects Of Salinity And Ph Change On The Physiology Of An Estuarine Fish Species, Fundulus Heteroclitus Heteroclitus, Shauna M. Tietze

Electronic Theses and Dissertations

Sapelo Island, Georgia is a pristine environment characterized by many variable salt marsh habitats. Throughout the island, salinity ranges from freshwater to full strength seawater and acidity levels range from low pH (pH < 4.4) to above neutral (pH > 8). Fundulus heteroclitus (Mummichog) is a resident organism of the salt marsh environment that has evolved coping strategies to withstand fluctuations in temperature, salinity and pH. Field and laboratory studies have shown F. heteroclitus to exhibit broad tolerance of individual environmental stressors; however, there is limited information on their ability to handle multiple stressors simultaneously. The goal of this study was to determine if exposure to low …