Life Sciences Commons^™

Nutrition Education To Minimize Health Risk: Approaches For Teaching College Students And Female High School Athletes, Katie Nicole Brown

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Obesity, heart disease, and osteoporosis are among the major health concerns facing Americans today. Obesity, elevated blood cholesterol, and even atherosclerosis are affecting America’s children and adolescents and putting them at increased risk for heart disease later in life. Similarly, low bone mineral density is seen among some adolescents including female athletes, increases their risk for immediate and lifetime fracture. Female athletes who do not consume enough calories to compensate for calories expended during physical activity are in a state of low energy availability which can lead to menstrual dysfunction, as well as bone loss, and injury (the female athlete …

Local And Regional Drivers Of Biodiversity: From Life-History Traits To System-Level Properties, Sarah R. Supp

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Biodiversity research includes the study of where species occur, the commonness and rarity of species, the number of species, and the diversity of life-history traits that occur in a single location, or community. Research is increasingly recognizing that a combination of local and regional scale processes influence community dynamics over ecological and evolutionary time-scales. However, ecologists currently lack a comprehensive understanding of the mechanisms driving biodiversity in different systems and at different spatial scales. This presents a critical problem because without understanding the important mechanisms that determine and maintain biodiversity, it is difficult to accurately predict community response to environmental …

Characterization And Potential Utility Of Porcine Trophoblast-Derived Stem-Like Cells, Edison A. Suasnavas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In mammalian pregnancy, the placenta is a very important organ which helps to establish a healthy pregnancy. Its functions could be described in four points: 1) It allows the fetus to receive gases and nutrients from the mother in a safe way; 2) it lets the fetus get rid of waste through the mother's kidneys; 3) it releases essential pregnancy-related hormones and growth factors that let the uterus hold the fetus; 4) it secretes immune response regulators to give the fetus immune protection against the mother. Abnormalities in the placenta can be the cause of death to the fetus. Mammalian …

Characterization Of Lactose Monolaurate For Its Antimicrobial And Emulsification Properties And Its Effect On Crystallization Behavior Of Anhydrous Milk Fat, Ashwini Wagh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A major class of synthetic emulsifiers used in food is sucrose esters. However, the use of lactose esters in foods is not very well known. Lactose monolaurate was characterized in our laboratory with respect to its antimicrobial, emulsification and fat crystallization modifier properties. The benefits of this study were multifold. A single compound was explored for multiple functionalities. Enzymatic synthesis and characterization of lactose monolaurate was carried out in the laboratory using food grade solvents. This process utilized lactose, a major byproduct of the dairy industry which is cheaper than sucrose.

In the first study, lactose monolaurate was explored for …

Development Of Sediment Budgets At Multiple Scales, Susannah O'Brien Erwin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Channel morphology in alluvial rivers is determined by the flow of water and sediment, and the characteristics of the materials making up the channel boundary. Many modern river management problems depend upon our ability to predict channel behavior in response to changes in the delivery of water or sediment. Sediment delivery to a river may be altered by natural or human-caused changes, such as changes in land use in the watershed, construction and operations of dam, forest fires, or climate change. Understanding and predicting the effects of these alterations is important because changes in sediment supply and transport may alter …

Assessing The Importance Of Sequencing Laboratory Welding Practicums, Malcolm Riley Rose

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to determine if having fewer operator-controlled variables during welding will improve secondary students' ability to meet weld quality standards for an AWS 1F test. Two different welding processes, Gas Metal Arc Welding and Shielded Metal Arc welding, were used in the research. The population of this study (N = 71), participants were randomly assigned into one of four treatment groups. The study was conducted over six class periods. Participants completed a welding pretest, taught safety procedures followed by welding instruction of both GMAW and SMAW process. Practice sessions were given for each welding …

Effects Of Integrating Mathematical Concepts Into An Animal Science Curriculum, Andrea Rae Clark

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Nationwide, mathematical scores have been a topic of concern among elementary and secondary educators for many years. Teaching contextualized math has been found to be effective and includes providing a direct application to real-life scenarios rather than teaching linear equations and algebraic principles outside of their application.

A study was conducted measuring the effects of integrating mathematical skills in an animal science curriculum. All students were taught a unit of instruction about animal nutrition and feeding. The control group received a typical nutrition unit and the treatment group received the same unit of instruction with the addition of mathematical skill …

Greater Sage-Grouse Habitat Selection And Use Patterns In Response To Vegetation Management Practices In Northwestern Utah, Stephanie E. Graham

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Greater sage-grouse (Centrocercus urophasianus; sage-grouse) are a sagebrush obligate species and an indicator of sagebrush (Artemisia spp.) habitat quality. Sage-grouse populations have declined across western North America. Fragmentation of landscapes and habitat loss have been identified as factors that negatively impact sage-grouse populations. Wildfires can increase the distribution of invasive plants and contribute to fragmentation and habitat loss across sagebrush ecosystems. Greenstripping has been identified as a technique to reduce the threat of wildfire and subsequent spread of invasive species. Forage kochia (Bassia prostrata) is a semi-shrub that contains a high moisture content year-round, high …

Kisspeptins - Expression, Purification, And Biological Assays, Mathew Puthayathu Kurian

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Kisspeptins are hormones involved in the onset of puberty in adolescents and maintenance of reproductive function in adults. Defects in kisspeptin signaling are associated with many reproductive disorders. Kisspeptins were originally discovered as metastasis suppressors and have been found to suppress the metastasis of certain cancers. Unfortunately, many aspects of kisspeptin action at a cellular level remain unknown. Kisspeptins, therefore, are attractive candidates for large-scale production for use as research and therapeutic reagents. We developed a simple and cost effective method to express and purify kisspeptins in large quantities. We also tested the biological activity of kisspeptins on cells expressing …

Characterization Of The Product Specificity And Kinetic Mechanism Of Protein Arginine Methyltransferase 1, Shanying Gui

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protein enzymes perform a vast array of functions within living organisms, catalyzing various metabolic reactions including DNA replication, DNA repair, protein synthesis, etc. In order to maintain proper cellular functions, enzymes need to be accurately regulated under different circumstances. Specifically, enzymes can be modified after their creation to give them additional functions. These modifications can do a variety of things including activating (turning on) or inactivating (turning off) an enzyme, changing what proteins or molecules can interact with the enzyme, changing the enzyme’s location in the cell, and/or targeting the enzyme for destruction. This dissertation focuses on a single class …

Strategic Approaches To Develop Optimal Feeding Program Of Brown Midrib Corn Silage To Lactating Dairy Cows In The Intermountain West, Michael Shane Holt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In two lactation studies reported in this dissertation, it was hypothesized that feeding 35% brown midrib corn silage (BMRCS) and 25% alfalfa hay (dry matter basis) would result in increased dry matter intake (DMI) around peak lactation compared with feeding conventional corn silage (CCS), causing longer peak milk production, and that feeding dairy cows in early lactation a 16% crude protein diet with fair quality alfalfa hay (FAH) in BMR-based diets would maintain milk production, reduce urinary N excretion, and improve N efficiency compared to those fed high quality alfalfa hay ( …

Integrated Management Of Downy Brome (Bromus Tectorum L.) Infested Rangeland, Heather Elwood

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Invasive weed species are a threat to the health and functionality of many rangeland systems. Downy brome (Bromus tectorum) is an invasive annual grass that affects the productivity of rangelands by decreasing the grazing capacity for livestock as well as altering the wildfire cycle and competing against more desirable vegetation for limited resources.

In 2006, an Invasive Plant Management Plan and Environmental Assessment was approved for Dinosaur National Monument, calling for prioritization of invasive species management on high value wildlife habitat, vector areas, and for species with a high ecological impact. The Cub Creek Watershed was identified as a priority …

A Multiday Record-Assisted Fruit, Vegetable, And Snack Questionnaire To Assess Intake Among Fourth And Fifth Grade Students, Anne B. Lambert

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Assessing FV intake among young children is difficult and can be a time-consuming, resource-intensive, and burdensome. The aim of this study was to create a simple, self-assessment tool to estimate and detect changes in FV consumption among fourth and fifth graders. Such a tool would ease the complications and resources of other diet assessment options that are used to measure FV consumption when implementing school-based nutrition programs.

The questionnaire provided a moderately valid estimate of lunchtime FV consumption, but it was inconsistent at detecting small, yet important increases in FV consumption between time points. The ability of the questionnaire to …

Apoptotic And Epigenetic Induction Of Embryo Failure Following Somatic Cell Nuclear Transfer, Aaron Patrick Davis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The cloning of domestic species has tremendous potential, as the technology can be used in selective breeding, conservation, and the production of transgenic animals. The technique of cloning involves the transplant of DNA from a cell to a recipient gamete. Following transfer to a surrogate, the cloned embryo may successfully complete development to a live offspring. Despite intensive research, the success rate of cloning remains prohibitively low, and the potential benefits of cloning have not yet been realized. Embryos produced from cloning suffer from high rates of embryo degradation, implantation failure, and abortion. The goal of this dissertation research project …

The Micronutrient Profile Of The Typical American Diet Enhances Colorectal Carcinogenesis, Stephany Del Carmen Perez Monsanto

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The typical Western dietary pattern is characterized by the consumption of energy-dense, nutrient-poor foods and has been linked to increased risk of colorectal cancer (CRC). Our research group previously developed the total Western diet (TWD) that emulates typical human dietary intakes of macro- (carbohydrates, proteins, and fats) and micronutrients (vitamins and minerals) on an energy density basis for rodents. In the present study, we sought to determine the impact of TWD on biomarkers of metabolic syndrome and obesity in comparison to a commercial 45% fat diet used for models of diet-induced obesity (DIO diet) and the standard basal AIN93G diet, …

Diet-Induced Obesity Decreases Liver Iron Stores In Mice Fed Iron Deficient, Adequate, Or Excessive Diets, Brett J. Healy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Epidemiological and observational evidence suggests that obesity is related to poor Fe status. To determine interactions between obesity, dietary Fe intake and Fe status; male, weanling C57BL/6J mice were fed either high fat diets to induce obesity or a standard diet for 16 weeks. Fe concentrations of both the high fat or control diet (4.5 vs 3.8 kcal/g) were set at: 5, 50 or 500 mg Fe/kg diet. Mice fed the high fat diets had significantly higher percentage body fat (17.9%) compared to mice fed control diets (5.3%, P<0.001). Among obese mice, dietary Fe levels did not significantly influence body composition. Conversely among lean mice, mice fed the iron excessive diet had significantly less fat mass when compared to mice fed the iron deficient diet (P<0.05). Obesity and/or dietary Fe concentration did not significantly affect plasma Fe levels. ANOVA analysis showed significant effects of diet-induced obesity, dietary Fe and an interaction between both factors on liver Fe levels (P< 0.05). Obese mice had significantly lowered liver Fe levels compared to lean cohorts fed the same amount of dietary Fe (P<0.05 for all comparisons). Moreover, lean mice fed the Fe deficient diet (5 mg Fe/kg diet) had similar liver Fe levels (127 mg Fe/kg ± 0.04) compared to obese mice fed the 50 mg Fe/kg diet (132 mg Fe/kg ± 0.05). These data suggest that obesity, independent of dietary Fe intake, influences liver Fe stores.

Bioactivities Of Milk Polar Lipids In Influencing Intestinal Barrier Integrity, Systemic Inflammation, And Lipid Metabolism, Albert Lihong Zhou

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of lactation is for nutrient provision and also importantly for protection from various environmental stressors. Milk polar lipids reduce cholesterol, protect against bacterial infection, reduce inflammation and help maintain gut integrity. Dynamic interactions within dietary fat, lipid metabolism, gut permeability and inflammatory cytokines remain unclear in the context of obesity and systemic inflammation. A rat model and three mouse models were developed to test the hypotheses that dietary milk polar lipids may affect lipid metabolism and intestinal integrity and may protect against systemic inflammation in the context of stressful diet, systemic inflammation, and obesity.

The milk polar lipids …

Comparison Of Topographic Surveying Techniques In Streams, Sara G. Bangen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Bonneville Power Administration (BPA) Fish and Wildlife Program (FWP) mitigates impacts, including mortality, from hydroelectric dams in the Columbia River Basin for ESA-listed salmon and steelhead populations and other species of special concern. Given the extensive economic resources invested in mitigation and the incredible diversity of in-stream habitat across the Columbia River basin, questions have arisen about which sampling strategies are most tractable across the entire Columbia River Watershed, but also produce datasets that allow researchers to answer meaningful questions about salmonid populations and trends in habitat. In response to these issues, the NOAA Integrated Status and Effectiveness Monitoring …

Increased Production And Extraction Efficiency Of Triacylglycerides From Microorganisms And An Enhanced Understanding Of The Pathways Involved In The Production Of Triacylglycerides And Fatty Alcohols, Robert M. Willis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The continued increase in the demand for fossil fuels combined with their ever dwindling supply has prompted the search for a suitable alternative fuel. The research contained within this dissertation seeks to increase the lipid (fat) content of cellular feedstocks, improve extraction efficiencies of lipids, and to understand the pathways involved in the production of fatty alcohols and triacylglycerides, compounds commonly used in many industrial processes, from microbial feedstocks. This work has been done in an attempt to increase the overall economic viability of microbial biofuels production.

The production of biofuels from microalgae used as a feedstock allows for the …

Effects Of Non-Surface-Disturbing Restoration Treatments On Native Grass Revegetation And Soil Seed Bank Composition In Cheatgrass-Invaded Sagebrush-Steppe Ecosystems, Alexandra D. Reinwald

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The sagebrush-steppe communities of the Great Basin have been dramatically transformed by the invasion of the non-native annual grass cheatgrass. In many areas of the Great Basin, this invasion has resulted in the loss of native plant species and ultimately the conversion to cheatgrass-dominated communities. As healthy sagebrush communities provide multiple ecosystem services such as diverse wildlife habitat, forage for cattle grazing, and water filtration, restoration of these communities is a high priority to landowners and land management agencies. Established perennial grasses can successfully compete with non-native annual grasses and increase the resistance of plant communities to invasion by non-native …