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Articles 1 - 22 of 22
Full-Text Articles in Life Sciences
Sequencing Of Aspergillus Nidulans And Comparative Analysis With A. Fumigatus And A. Oryzae, James E. Galagan, Sarah E. Calvo, Christina Cuomo, Li-Jun Ma, Jennifer R. Wortman, Serafim Batzoglou, Su-In Lee, Meray Baştürkmen, Christina C. Spevak, Vladimir Kapitonov, Jerzy Jurka, Claudio Scazzocchio, Mark Farman, Jonathan Butler, Seth Purcell, Steven D. Harris, Gerhard H. Braus, Oliver Draht, Silke Busch, Christophe D'Enfert, Christiane Bouchier, Gustavo H. Goldman, Deborah Bell-Pedersen, Sam Griffiths-Jones, John H. Doonan, Jaehyuk Yu, Kay Vienken, Arnab Pain, Michael Freitag, Eric U. Selker, David B. Archer, Miguel Á. Peñalva, Berl R. Oakley, Michelle Momany, Toshihiro Tanaka, Toshitaka Kumagai, Kiyoshi Asai, Masayuki Machida, William C. Nierman, David W. Denning, Mark Caddick, Michael Hynes, Mathieu Paoletti, Reinhard Fischer, Bruce Miller, Paul Dyer, Matthew S. Sachs, Stephen A. Osmani, Bruce W. Birren
Sequencing Of Aspergillus Nidulans And Comparative Analysis With A. Fumigatus And A. Oryzae, James E. Galagan, Sarah E. Calvo, Christina Cuomo, Li-Jun Ma, Jennifer R. Wortman, Serafim Batzoglou, Su-In Lee, Meray Baştürkmen, Christina C. Spevak, Vladimir Kapitonov, Jerzy Jurka, Claudio Scazzocchio, Mark Farman, Jonathan Butler, Seth Purcell, Steven D. Harris, Gerhard H. Braus, Oliver Draht, Silke Busch, Christophe D'Enfert, Christiane Bouchier, Gustavo H. Goldman, Deborah Bell-Pedersen, Sam Griffiths-Jones, John H. Doonan, Jaehyuk Yu, Kay Vienken, Arnab Pain, Michael Freitag, Eric U. Selker, David B. Archer, Miguel Á. Peñalva, Berl R. Oakley, Michelle Momany, Toshihiro Tanaka, Toshitaka Kumagai, Kiyoshi Asai, Masayuki Machida, William C. Nierman, David W. Denning, Mark Caddick, Michael Hynes, Mathieu Paoletti, Reinhard Fischer, Bruce Miller, Paul Dyer, Matthew S. Sachs, Stephen A. Osmani, Bruce W. Birren
Department of Plant Pathology: Faculty Publications
The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. Here we report the genome sequence of the model organism Aspergillus nidulans, and a comparative study with Aspergillus fumigatus, a serious human pathogen, and Aspergillus oryzae, used in the production of sake, miso, and soy sauce. Our analysis of genome structure provided a quantitative evaluation of forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in A. fumigatus and A. oryzae. Our analysis of sequence …
Complete Deletion Of Wheat Streak Mosaic Virus Hc-Pro: A Null Mutant Is Viable For Systemic Infection, Drake C. Stenger, Roy C. French, Frederick E. Gildow
Complete Deletion Of Wheat Streak Mosaic Virus Hc-Pro: A Null Mutant Is Viable For Systemic Infection, Drake C. Stenger, Roy C. French, Frederick E. Gildow
Department of Plant Pathology: Faculty Publications
A Wheat streak mosaic virus (WSMV) genome lacking HC-Pro was constructed and confirmed by reverse transcription-PCR to systemically infect wheat, oat, and corn. Coupled in vitro transcription/translation reactions indicated that WSMV P1 proteinase cleaved the polyprotein at the P1/P3 junction of the HC-Pro null mutant. The WSMV HC-Pro null mutant was competent for virion formation, but the virus titer was reduced 4.5-fold relative to that of the wild type. Collectively, these results indicate that WSMV HC-Pro is dispensable for replication and movement, two essential processes that are disrupted by point and small-insertion mutations introduced into potyvirus HC-Pro.
First Report Of Crown And Stem Rot Of Orchid (Orchis Palustris) Caused By Sclerotinia Minor, C. Eken, S. Ercişli, A. Eşitken, E. Demirci, G. Y. Yuen
First Report Of Crown And Stem Rot Of Orchid (Orchis Palustris) Caused By Sclerotinia Minor, C. Eken, S. Ercişli, A. Eşitken, E. Demirci, G. Y. Yuen
Department of Plant Pathology: Faculty Publications
Orchis palustris Jacq. is a wild orchid native to wetlands in eastern Anatolia. During June of 2003, near Erzurum, Turkey, a decline of this orchid was observed in several meadows that had been irrigated for forage production. Stems were chlorotic, wilted, and collapsed. There was a soft, watery rot at the crowns and lower stems. White mycelium and black sclerotia formed on necrotic stem and crown tissues. The fungus was isolated from sclerotia on potato dextrose agar (PDA) and identified as Sclerotinia minor Jagger on the basis of small sclerotia (0.5 to 2.5 mm long) scattered throughout the colonies (2). …
Reclassification Of Xanthomonas Campestris Pv. Citri (Ex Hasse 1915) Dye 1978 Forms A, B/C/D, And E As X. Smithii Subsp. Citri (Ex Hasse) Sp. Nov. Nom. Rev. Comb. Nov., X. Fuscans Subsp. Aurantifolii (Ex Gabriel 1989) Sp. Nov. Nom. Rev. Comb. Nov., And X. Alfalfae Subsp. Citrumelo (Ex Riker And Jones) Gabriel Et Al., 1989 Sp. Nov. Nom. Rev. Comb. Nov.; X. Campestris Pv Malvacearum (Ex Smith 1901) Dye 1978 As X. Smithii Subsp. Smithii Nov. Comb. Nov. Nom. Nov.; X. Campestris Pv. Alfalfae (Ex Riker And Jones, 1935) Dye 1978 As X. Alfalfae Subsp. Alfalfae (Ex Riker Et Al., 1935) Sp. Nov. Nom. Rev.; And ‘‘Var. Fuscans’’ Of X. Campestris Pv. Phaseoli (Ex Smith, 1987) Dye 1978 As X. Fuscans Subsp. Fuscans Sp. Nov., Norman W. Schaad, Elena Postnikova, George H. Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver
Reclassification Of Xanthomonas Campestris Pv. Citri (Ex Hasse 1915) Dye 1978 Forms A, B/C/D, And E As X. Smithii Subsp. Citri (Ex Hasse) Sp. Nov. Nom. Rev. Comb. Nov., X. Fuscans Subsp. Aurantifolii (Ex Gabriel 1989) Sp. Nov. Nom. Rev. Comb. Nov., And X. Alfalfae Subsp. Citrumelo (Ex Riker And Jones) Gabriel Et Al., 1989 Sp. Nov. Nom. Rev. Comb. Nov.; X. Campestris Pv Malvacearum (Ex Smith 1901) Dye 1978 As X. Smithii Subsp. Smithii Nov. Comb. Nov. Nom. Nov.; X. Campestris Pv. Alfalfae (Ex Riker And Jones, 1935) Dye 1978 As X. Alfalfae Subsp. Alfalfae (Ex Riker Et Al., 1935) Sp. Nov. Nom. Rev.; And ‘‘Var. Fuscans’’ Of X. Campestris Pv. Phaseoli (Ex Smith, 1987) Dye 1978 As X. Fuscans Subsp. Fuscans Sp. Nov., Norman W. Schaad, Elena Postnikova, George H. Lacy, Aaron Sechler, Irina V. Agarkova, Paul E. Stromberg, Verlyn K. Stromberg, Anne K. Vidaver
Department of Plant Pathology: Faculty Publications
Bacterial canker of citrus is a serious disease of citrus worldwide. Five forms of the disease have been described, cankers “A”, “B”, “C”, “D”, and “E”. Although considerable genetic diversity has been described among the causal agents of the five forms of citrus canker and supports multiple taxons, the causal agents currently are classified as pathovars citri (“A”), aurantifolii (“B/C/D”) and citrumelo (“E”) of a single species, Xanthomonas campestris pv. citri (or X. axonopodis pv. citri).To determine the taxonomic relatedness among strains of X. campestris pv. citri, we conducted DNA–DNA relatedness assays, sequenced the 16S-23S intergenic spacer (ITS) …
Plant Virus Hc-Pro Is A Determinant Of Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Frederick E. Gildow, Kempton M. Horken, Roy C. French
Plant Virus Hc-Pro Is A Determinant Of Eriophyid Mite Transmission, Drake C. Stenger, Gary L. Hein, Frederick E. Gildow, Kempton M. Horken, Roy C. French
Department of Plant Pathology: Faculty Publications
The eriophyid mite transmitted Wheat streak mosaic virus (WSMV; genus Tritimovirus, family Potyviridae) shares a common genome organization with aphid transmitted species of the genus Potyvirus. Although both tritimoviruses and potyviruses encode helper component-proteinase (HC-Pro) homologues (required for nonpersistent aphid transmission of potyviruses), sequence conservation is low (amino acid identity, ~16%), and a role for HC-Pro in semipersistent transmission of WSMV by the wheat curl mite (Aceria tosichella [Keifer]) has not been investigated. Wheat curl mite transmissibility was abolished by replacement of WSMV HC-Pro with homologues of an aphid transmitted potyvirus (Turnip mosaic virus), …
Aspergillus Nidulans UvsbAtr And ScaaNbs1 Genes Show Genetic Interactions During Recovery From Replication Stress And Dna Damage, Marcia Regina Von Zeska Kress Fagundes, Camile P. Semighini, Iran Malazavi, Marcela Savoldi, Joel Fernandes De Lima, Maria Helena De Souza Goldman, Steven D. Harris, Gustavo Henrique Goldman
Aspergillus Nidulans UvsbAtr And ScaaNbs1 Genes Show Genetic Interactions During Recovery From Replication Stress And Dna Damage, Marcia Regina Von Zeska Kress Fagundes, Camile P. Semighini, Iran Malazavi, Marcela Savoldi, Joel Fernandes De Lima, Maria Helena De Souza Goldman, Steven D. Harris, Gustavo Henrique Goldman
Department of Plant Pathology: Faculty Publications
The ATM/ATR kinases and the Mre11 (Mre11-Rad50-Nbs1) protein complex are central players in the cellular DNA damage response. Here we characterize possible interactions between Aspergillus nidulans uvsB- ATR and the Mre11 complex (scaANBS1). We demonstrate that there is an epistatic relationship between uvsBATR, the homolog of the ATR/MEC1 gene, and scaANBSI, the homolog of the NBS1/XRS2 gene, for both repair and checkpoint functions and that correct ScaANBS1 expression during recovery from replication stress depends on uvsBATR. In addition, we also show that the formation of UvsC foci during recovery …
Mutagenesis Of Β-1,3-Glucanase Genes In Lysobacter Enzymogenes Strain C3 Results In Reduced Biological Control Activity Toward Bipolaris Leaf Spot Of Tall Fescue And Pythium Damping-Off Of Sugar Beet, Jeffrey D. Palumbo, Gary Y. Yuen, C. Christine Jochum, Kristin Tatum, Donald Y. Kobayashi
Mutagenesis Of Β-1,3-Glucanase Genes In Lysobacter Enzymogenes Strain C3 Results In Reduced Biological Control Activity Toward Bipolaris Leaf Spot Of Tall Fescue And Pythium Damping-Off Of Sugar Beet, Jeffrey D. Palumbo, Gary Y. Yuen, C. Christine Jochum, Kristin Tatum, Donald Y. Kobayashi
Department of Plant Pathology: Faculty Publications
Lysobacter enzymogenes produces extracellular lytic enzymes capable of degrading the cell walls of fungi and oomycetes. Many of these enzymes, including β-1,3-glucanases, are thought to contribute to the biological control activity expressed by several strains of the species. L. enzymogenes strain C3 produces multiple extracellular β-1,3-glucanases encoded by the gluA, gluB, and gluC genes. Analysis of the genes indicates they are homologous to previously characterized genes in the related strain N4-7, each sharing >95% amino acid sequence identity to their respective counterparts. The gluA and gluC gene products encode enzymes belonging to family 16 glycosyl …
Green Fluorescent Protein Is Lighting Up Fungal Biology, J.M. Lorang, R.P. Tuori, J.P. Martinez, T.L. Sawyer, R.S. Redman, J.A. Rollins, T. J. Wolpert, K.B. Johnson, R. J. Rodriguez, M. B. Dickman, L. M. Ciuffetti
Green Fluorescent Protein Is Lighting Up Fungal Biology, J.M. Lorang, R.P. Tuori, J.P. Martinez, T.L. Sawyer, R.S. Redman, J.A. Rollins, T. J. Wolpert, K.B. Johnson, R. J. Rodriguez, M. B. Dickman, L. M. Ciuffetti
Department of Plant Pathology: Faculty Publications
Prasher (42) cloned a cDNA for the green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria in 1992. Shortly thereafter, to the amazement of many investigators, this gene or derivatives thereof were successfully expressed and conferred fluorescence to bacteria and Caenorhabditis elegans cells in culture (10, 31), followed by yeast (24, 39), mammals (40), Drosophila (66), Dictyostelium (23, 30), plants (28, 49), and filamentous fungi (54). The tremendous success of GFP as a reporter can be attributed to unique qualities of this 238- amino-acid, 27-kDa protein which absorbs light at maxima of 395 and 475 nm and emits light …
Proposed Guidelines For A Unified Nomenclature And Phylogenetic Analysis Of Type Iii Hop Effector Proteins In The Plant Pathogen Pseudomonas Syringae, Magdalen Lindeberg, John Stavrinides, Jeffrey H. Chang, James R. Alfano, Alan Collmer, Jeffrey L. Dangl, Jean T. Greenberg, John W. Mansfield, David S. Guttman
Proposed Guidelines For A Unified Nomenclature And Phylogenetic Analysis Of Type Iii Hop Effector Proteins In The Plant Pathogen Pseudomonas Syringae, Magdalen Lindeberg, John Stavrinides, Jeffrey H. Chang, James R. Alfano, Alan Collmer, Jeffrey L. Dangl, Jean T. Greenberg, John W. Mansfield, David S. Guttman
Department of Plant Pathology: Faculty Publications
Pathovars of Pseudomonas syringae interact with their plant hosts via the action of Hrp outer protein (Hop) effector proteins, injected into plant cells by the type III secretion system (TTSS). Recent availability of complete genome sequences for a number of P. syringae pathovars has led to a significant increase in the rate of effector discovery. However, lack of a systematic nomenclature has resulted in multiple names being assigned to the same Hop, unrelated Hops designated by the same alphabetic character, and failure of name choices to reflect consistent standards of experimental confirmation or phylogenetic relatedness. Therefore, specific experimental and bioinformatic …
Impact Of Reduced Lignin On Plant Fitness, Jeffrey F. Pedersen, Kenneth P. Vogel, Deanna L. Funnell
Impact Of Reduced Lignin On Plant Fitness, Jeffrey F. Pedersen, Kenneth P. Vogel, Deanna L. Funnell
Department of Plant Pathology: Faculty Publications
Lignin content of crop plants has been reduced by traditional plant breeding, natural and induced mutations, and insertion of transgenes. The effects of these genes and associated lower lignin content have been examined in terms of agricultural fitness or with regard to economically harvestable yields of useful plant products, or, in the case of some perennial species, survivability over multiple years. In general, crop yields are depressed by significant reductions in lignin content. Other negative effects observed in plants with lowered lignin contents include lodging and reduction of long-term survival of some perennial species. However, the interactions of genes involved …
Registration Of Common Bacterial Blight Resistant Pinto Bean Germplasm Line Abcp-8, N. Mutlu, P.N. Miklas, J. R. Steadman, A. K. Vidaver, Dale T. Lindgren, J. Reiser, D.P. Coyne, M. A. Pator-Corrales
Registration Of Common Bacterial Blight Resistant Pinto Bean Germplasm Line Abcp-8, N. Mutlu, P.N. Miklas, J. R. Steadman, A. K. Vidaver, Dale T. Lindgren, J. Reiser, D.P. Coyne, M. A. Pator-Corrales
Department of Plant Pathology: Faculty Publications
Pinto bean (Phaseolus vulgaris L.) germplasm line ABCP-8 (Reg. no. GP-237, PI 635118) was developed by the University of Nebraska Agricultural Research Division in cooperation with USDA-ARS and released in 2004. This line was bred specifically for enhanced resistnace to common bacterial blight [caused by Xanthomonas campestris pv. phaseoli (Smith) Dye]. Pinto and other dry bean market classes (dark red kidney, great northern, navy, etc.) grown in the U.S.A. east of the continental divide are often attacked by common bacterial blight, a seed-transmitted disease that causes up to 40% yield loss in susceptible cultivars as well as reduction of …
Dna Molecules And Polypeptides Of Pseudomonas Siringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 6,852,835 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski
Dna Molecules And Polypeptides Of Pseudomonas Siringae Hrp Pathogenicity Island And Their Uses: U.S. Patent No. Us 6,852,835 B2, Alan Collmer, James R. Alfano, Amy O. Charkowski
Department of Plant Pathology: Faculty Publications
One aspect of the present invention relates to isolated nucleic acid molecules (i) encoding proteins or polypeptides of Pseudomonas CEL and EEL genomic regions, (ii) nucleic acid molecules which hybridize thereto under stringent conditions, or (iii) nucleic acid molecules that include a nucleotide sequence which is complementary to the nucleic acid molecules of (i) and (ii). Expression vectors, host cells, and transgenic plants which include the DNA molecules of the present invention are also disclosed. Another aspect relates to the isolated proteins or polypeptides and compositions containing the same. The nucleic acid molecules and proteins of the present invention can …
Polarisome Meets Spitzenkö̈Rper: Microscopy, Genetics, And Genomics Converge, Steven D. Harris, Nick D. Read, Robert W. Roberson, Brian Shaw, Stephan Seiler, Mike Plamann, Michelle Momany
Polarisome Meets Spitzenkö̈Rper: Microscopy, Genetics, And Genomics Converge, Steven D. Harris, Nick D. Read, Robert W. Roberson, Brian Shaw, Stephan Seiler, Mike Plamann, Michelle Momany
Department of Plant Pathology: Faculty Publications
The impact of filamentous fungi on human welfare has never been greater. Fungi are acknowledged as the most economically devastating plant pathogens (1) and are attaining increasing notoriety for their ability to cause life-threatening infections in humans (57, 71), and fungal products sustain a billion dollar manufacturing industry (70). The tools available to study filamentous fungi are more sophisticated than ever and include the complete annotated genome sequences of multiple filamentous fungi (12), resources being made available through various functional genomics projects, and advanced bioimaging methods, including high-resolution live-cell imaging (20, 32) and electron tomography (19, 50). The increasing impact …
Mutual Interference Between Genomic Rna Replication And Subgenomic Mrna Transcription In Brome Mosaic Virus, Valery Z. Grdzelishvili, Hernan Garcia-Ruiz, Tokiko Watanabe, Paul Ahlquist
Mutual Interference Between Genomic Rna Replication And Subgenomic Mrna Transcription In Brome Mosaic Virus, Valery Z. Grdzelishvili, Hernan Garcia-Ruiz, Tokiko Watanabe, Paul Ahlquist
Department of Plant Pathology: Faculty Publications
Replication by many positive-strand RNA viruses includes genomic RNA amplification and subgenomic mRNA (sgRNA) transcription. For brome mosaic virus (BMV), both processes occur in virus-induced, membrane- associated compartments, require BMV replication factors 1a and 2a, and use negative-strand RNA3 as a template for genomic RNA3 and sgRNA syntheses. To begin elucidating their relations, we examined the interaction of RNA3 replication and sgRNA transcription in Saccharomyces cerevisiae expressing 1a and 2a, which support the full RNA3 replication cycle. Blocking sgRNA transcription stimulated RNA3 replication by up to 350%, implying that sgRNA transcription inhibits RNA3 replication. Such inhibition was independent of the …
No Political Interference In Us Agricultural Grants, Anne Vidaver
No Political Interference In Us Agricultural Grants, Anne Vidaver
Department of Plant Pathology: Faculty Publications
I write on behalf of several former chief scientists in charge of the US Department of Agriculture’s National Research Initiative (NRI) competitive grants program. We wish to clarify part of your Editorial “A chance for growth” (Nature 432, 257; 2004). The Editorial could be interpreted as suggesting that the department’s competitive peer-reviewed research programs are influenced by political interests. Such interests have played a part in dictating the general areas in which to conduct research, but as chief scientists in the competitive programs area, we did not observe interference with the peer-review process itself.
Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten
Chlorovirus: A Genus Of Phycodnaviridae That Infects Certain Chlorella-Like Green Algae, Ming Kang, David Dunigan, James L. Van Etten
Department of Plant Pathology: Faculty Publications
Taxonomy: Chlorella viruses are assigned to the family Phycodnaviridae, genus Chlorovirus, and are divided into three species: Chlorella NC64A viruses, Chlorella Pbi viruses and Hydra viridis Chlorella viruses. Chlorella viruses are large, icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorella-like green algae. The type member is Paramecium bursaria chlorella virus 1 (PBCV-1).
Physical properties: Chlorella virus particles are large (molecular weight ~1 × 109 Da) and complex. The virion of PBCV-1 contains more than 100 different proteins; the major capsid protein, Vp54, comprises ~40% of the virus protein. Cryoelectron microscopy and three-dimensional image reconstruction of PBCV-1 virions …
Morphogenesis In Germinating Fusarium Graminearum Macroconidia, Steven D. Harris
Morphogenesis In Germinating Fusarium Graminearum Macroconidia, Steven D. Harris
Department of Plant Pathology: Faculty Publications
Fusarium graminearum (teleomorph Gibberella zeae) is a significant pathogen of wheat and corn. F. graminearum forms multicellular macroconidia that play an important role in dissemination of the disease. The spatial pattern of morphogenesis in germinating macroconidia is described. Germ tubes preferentially emerge from the apical cells in a bipolar pattern that appears to be common to filamentous fungi. Chitin deposition occurs at two locations: the spore apices and cortical regions of macroconidial cells that subsequently produce a germ tube. The spatial pattern of morphogenesis requires the presence of functional microtubules, which may be responsible for the transport of key …
Proline Suppresses Apoptosis In The Fungal Pathogen Colletotrichum Trifolii, Changbin Chen, Martin B. Dickman
Proline Suppresses Apoptosis In The Fungal Pathogen Colletotrichum Trifolii, Changbin Chen, Martin B. Dickman
Department of Plant Pathology: Faculty Publications
The role of reactive oxygen species (ROS) in cell communication, control of gene expression, and oxygen sensing is well established. Inappropriate regulation of ROS levels can damage cells, resulting in a diseased state. In Colletotrichum trifolii, a fungal pathogen of alfalfa, the mutationally activated oncogenic fungal Ras (DARas) elevates levels of ROS, causing abnormal fungal growth and development and eventual apoptotic-like cell death but only when grown under nutrient-limiting conditions. Remarkably, restoration to the wild-type phenotype requires only proline. Here, we describe a generally unrecognized function of proline: its ability to function as a potent antioxidant and inhibitor of …
Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Department of Plant Pathology: Faculty Publications
A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil samples. No regulated pest species were recovered during this survey. Meloidogyne juveniles extracted from soil were identified by mitochondrial and 18S ribosomal molecular markers. Eighty-two DNA sequences representing the two marker regions for Meloidogyne species were submitted to GenBank to facilitate evaluation of marker variability. Sufficient 18S variation was observed among some Meloidogyne species to aid in identification; however, nucleotide sequence from …
The Glycosphingolipid Globotriaosylceramide In The Metastatic Transformation Of Colon Cancer, Olga Kovbasnjuk, Rakhilya Mourtazina, Boris Baibakov, Thomas Wang, Christian Elowsky, Michael A. Choti, Anne Kane, Mark Donowitz
The Glycosphingolipid Globotriaosylceramide In The Metastatic Transformation Of Colon Cancer, Olga Kovbasnjuk, Rakhilya Mourtazina, Boris Baibakov, Thomas Wang, Christian Elowsky, Michael A. Choti, Anne Kane, Mark Donowitz
Department of Plant Pathology: Faculty Publications
The most devastating aspect of cancer is the emergence of metastases. Thus, identification of potentially metastatic cells among a tumor cell population and the underlying molecular changes that switch cells to a metastatic state are among the most important issues in cancer biology. Here we show that, although normal human colonic epithelial cells lack the glycosphingolipid globotriaosylceramide (Gb3), this molecule is highly expressed in metastatic colon cancer. In addition, a subpopulation of cells that are greatly enriched in Gb3 and have an invasive phenotype was identified in human colon cancer cell lines. In epithelial cells in culture, …
Genome Sequences Of Agropyron Mosaic Virus And Hordeum Mosaic Virus Support Reciprocal Monophyly Of The Genera Potyvirus And Rymovirus In The Family Potyviridae, Roy C. French, D.C. Stenger
Genome Sequences Of Agropyron Mosaic Virus And Hordeum Mosaic Virus Support Reciprocal Monophyly Of The Genera Potyvirus And Rymovirus In The Family Potyviridae, Roy C. French, D.C. Stenger
Department of Plant Pathology: Faculty Publications
Assignment of mite-transmitted species to the genus Rymovirus (family Potyviridae) has changed several times, and the status of the genus has been questioned. To address this issue, complete genome sequences of the rymoviruses Agropyron mosaic virus (AgMV) and Hordeum mosaic virus (HoMV) were determined. AgMV (9540 nucleotides) and HoMV (9463 nucleotides) each encode a single polyprotein with proteinase cleavage sites demarcating protein products characteristic of monopartite species of the family Potyviridae. Of the described species of Potyviridae, AgMV and HoMV are most closely related to each other (68.5% nucleotide and 71.6% amino acid sequence identity) and equidistant …
Chlorella Virus Marburg Topoisomerase Ii: High Dna Cleavage Activity As A Characteristic Of Chlorella Virus Type Ii Enzymes, Jennifer S. Dickey, Tae-Jin Choi, James L. Van Etten, Neil Osheroff
Chlorella Virus Marburg Topoisomerase Ii: High Dna Cleavage Activity As A Characteristic Of Chlorella Virus Type Ii Enzymes, Jennifer S. Dickey, Tae-Jin Choi, James L. Van Etten, Neil Osheroff
Department of Plant Pathology: Faculty Publications
Although the formation of a covalent enzyme-cleaved DNA complex is a prerequisite for the essential functions of topoisomerase II, this reaction intermediate has the potential to destabilize the genome. Consequently, all known eukaryotic type II enzymes maintain this complex at a low steady-state level. Recently, however, a novel topoisomerase II was discovered in Paramecium bursaria chlorella virus-1 (PBCV-1) that has an exceptionally high DNA cleavage activity [Fortune et al. (2001) J. Biol. Chem. 276, 24401-24408]. If robust DNA cleavage is critical to the physiological functions of chlorella virus topoisomerase II, then this remarkable characteristic should be …