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Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Department of Plant Pathology: Faculty Publications
A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil samples. No regulated pest species were recovered during this survey. Meloidogyne juveniles extracted from soil were identified by mitochondrial and 18S ribosomal molecular markers. Eighty-two DNA sequences representing the two marker regions for Meloidogyne species were submitted to GenBank to facilitate evaluation of marker variability. Sufficient 18S variation was observed among some Meloidogyne species to aid in identification; however, nucleotide sequence from …