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A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
Department of Plant Pathology: Faculty Publications
A polymerase chain reaction (PCR) method for discriminating Meloidogyne incognita, M. arenaria, M. javanica, M. hapla, and M. chitwoodi was developed. Single juveniles were ruptured in a drop of water and added directly to a PCR reaction mixture in a microcentrifuge tube. Primer annealing sites were located in the 3' portion of the mitochondrial gene coding for cytochrome oxidase subunit II and in the 16S rRNA gene. Following PCR amplification, fragments of three sizes were detected. The M. incognita and M. javanica reactions produced a 1.7-kb fragment; the M. arenaria reaction, a 1.1-kb fragment; and the …