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Full-Text Articles in Life Sciences
Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Incorporating Molecular Identification Of Meloidogyne Spp. Into A Large-Scale Regional Nematode Survey, Thomas O. Powers, P. G. Mullin, T. S. Harris, L. A. Sutton, R. S. Higgins
Department of Plant Pathology: Faculty Publications
A regional nematode survey of potato fields was conducted in the central United States during 2002 and 2003. The survey encompassed seven states and included a morphological and molecular examination of nematodes of regulatory concern from 1,929 soil samples. No regulated pest species were recovered during this survey. Meloidogyne juveniles extracted from soil were identified by mitochondrial and 18S ribosomal molecular markers. Eighty-two DNA sequences representing the two marker regions for Meloidogyne species were submitted to GenBank to facilitate evaluation of marker variability. Sufficient 18S variation was observed among some Meloidogyne species to aid in identification; however, nucleotide sequence from …
Morphological And Molecular Characterization Of Meloidogyne Mayaguensis Isolates From Florida, J. Brito, Thomas O. Powers, P. G. Mullin, Florida Department Of Agriculture And Consumer Services N., D. W. Dickson
Morphological And Molecular Characterization Of Meloidogyne Mayaguensis Isolates From Florida, J. Brito, Thomas O. Powers, P. G. Mullin, Florida Department Of Agriculture And Consumer Services N., D. W. Dickson
Department of Plant Pathology: Faculty Publications
The discovery of Meloidogyne mayaguensis is confirmed in Florida; this is the first report for the continental United States. Meloidogyne mayaguensis is a virulent species that can reproduce on host cultivars bred for nematode resistance. The perineal patterns of M. mayaguensis isolates from Florida show morphological variability and often are similar to M. incognita. Useful morphological characters for the separation of M. mayaguensis from M. incognita from Florida are the male stylet length values (smaller for M. mayaguensis than M. incognita) and J2 tail length values (greater for M. mayaguensis than M. incognita). Meloidogyne mayaguensis values for …
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
Department of Plant Pathology: Faculty Publications
A polymerase chain reaction (PCR) method for discriminating Meloidogyne incognita, M. arenaria, M. javanica, M. hapla, and M. chitwoodi was developed. Single juveniles were ruptured in a drop of water and added directly to a PCR reaction mixture in a microcentrifuge tube. Primer annealing sites were located in the 3' portion of the mitochondrial gene coding for cytochrome oxidase subunit II and in the 16S rRNA gene. Following PCR amplification, fragments of three sizes were detected. The M. incognita and M. javanica reactions produced a 1.7-kb fragment; the M. arenaria reaction, a 1.1-kb fragment; and the …