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Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Effect Of Organic Matter Decomposition Level On Bacterial Species Diversity And Composition In Relationship To Pythium Damping-Off Severity, Michael J. Boehm, L.V. Mdden, H.A.J. Hoitink
Effect Of Organic Matter Decomposition Level On Bacterial Species Diversity And Composition In Relationship To Pythium Damping-Off Severity, Michael J. Boehm, L.V. Mdden, H.A.J. Hoitink
Department of Plant Pathology: Faculty Publications
Rhizosphere bacteria were isolated from root tip segments of cucumber seedlings grown in a suppressive, slightly decomposed light-colored peat mix, a conducive, more decomposed dark-colored peat mix, and a suppressive dark peat mix amended with composted hardwood bark. The bacteria were identified by a gas chromatographic fatty acid methyl ester analysis. The total number of taxa recovered from a single root tip segment ranged from 9 to 18. No single taxon predominated on all root tip segments harvested from any of the mixes. The highest relative population density reached by a given taxon on any root tip segment was 45%. …
Leptospira Genomes Are Modified At 5'-Gtac, David Ralph, Quideng Que, James L. Van Etten, Michael Mcclelland
Leptospira Genomes Are Modified At 5'-Gtac, David Ralph, Quideng Que, James L. Van Etten, Michael Mcclelland
Department of Plant Pathology: Faculty Publications
Genomic DNAs of 14 strains from seven species of the spirochete Leptospira were resistant to cleavage by the restriction endonuclease RsaI 1 (5'-GTAC). A modified base comigrating with m4C was detected by chromatography. Genomic DNAs from other spirochetes, Borrelia group VS461, and Serpulina strains were not resistant to h a 1 digestion. Modification at 5'-GTAm4C may occur in most or all strains of all species of Leptospira but not in all genera of spirochetes. Genus-wide DNA modification has rarely been observed in bacteria.
The Polymerase Chain Reaction And Plant Disease Diagnosis, Joan M. Henson, Roy C. French
The Polymerase Chain Reaction And Plant Disease Diagnosis, Joan M. Henson, Roy C. French
Department of Plant Pathology: Faculty Publications
The polymerase chain reaction (PCR) provides a simple, ingenious method to exponentially amplify specific DNA sequences by in vitro DNA synthesis. Three essential steps to PCR (Figure 1) include (a) melting of the target (b) annealing of two oligonucleotide primers to the denatured DNA strands, and (c) primer extension by a thermostable DNA polymerase (123). Newly synthesized DNA strands serve as targets for subsequent DNA synthesis as the three steps are repeated up to 50 times. The specificity of the method derives from the synthetic oligonucleotide primers, which base-pair to and define each end of the target sequence to be …
Evidence For Virus-Encoded Glycosylation Specificity, Ing-Nang Wang, Yu Li, Quideng Que, Meenakshi Bhattacharya, Leslie C. Lane, William G. Chaney, James L. Van Etten
Evidence For Virus-Encoded Glycosylation Specificity, Ing-Nang Wang, Yu Li, Quideng Que, Meenakshi Bhattacharya, Leslie C. Lane, William G. Chaney, James L. Van Etten
Department of Plant Pathology: Faculty Publications
Four spontaneously derived serologically distinct classes of mutants of the Paramecium bursaria chlorella virus (PBCV-1) were isolated using polydonal antiserum prepared against either intact PBCV-1 or PBCV-1-derived serotypes. The oligosaccharide(s) of the viral major capsid protein and two minor glycoproteins determined virus serological specificity. Normally, viral glycoproteins arise from host-specific glycosylation of viral proteins; the glycan portion can be altered only by growing the virus on another host or by mutations in glycosylation sites of the viral protein. Neither mechanism explains the changes in the glycan(s) of the PBCV-1 major capsid protein because all of the viruses were grown in …
The Future Of Agricultural Research, Roger Beachy, Susanne L. Huttner, Anne K. Vidaver
The Future Of Agricultural Research, Roger Beachy, Susanne L. Huttner, Anne K. Vidaver
Department of Plant Pathology: Faculty Publications
We enthusiastically support Philip H. Abelson’s call for substantially increased funding for basic agricultural research (Editorial, 28 Aug., p. 1187). However, he neglects the government’s critical role as gatekeeper; some federal regulatory policies are serious impediments to progress in the agricultural sciences. A subsequent editorial by Charles Arntzen, “Regulation of transgenic plants’ (4 Sept., p 1327), points out that research on genetically engineered plants is now subject to delays and extensive assessments that result from perceptions of public concern and not from scientific evidence of risk. when government’s research and regulatory policies conflict, the public loses twice-their investment in the …
Sequencing Two Dna Templates In Five Channels By Digital Compression, Michaela Nelson, Yanping Zhang, David L. Steffens, Reingard Grabherr, James L. Van Etten
Sequencing Two Dna Templates In Five Channels By Digital Compression, Michaela Nelson, Yanping Zhang, David L. Steffens, Reingard Grabherr, James L. Van Etten
Department of Plant Pathology: Faculty Publications
By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneousl in only flve reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2" - 1 ≥ 4f, describes conditions under which f DNA templates can be sequenced using n channels. Such compression sequencing is accurate and effcient, as demonstrated by manual 35S autoradiographic detection and automated on-line analysis using fluorescent-labeled primers. Symmetric 5:2 compression is especially useful when …
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
Department of Plant Pathology: Faculty Publications
A polymerase chain reaction (PCR) method for discriminating Meloidogyne incognita, M. arenaria, M. javanica, M. hapla, and M. chitwoodi was developed. Single juveniles were ruptured in a drop of water and added directly to a PCR reaction mixture in a microcentrifuge tube. Primer annealing sites were located in the 3' portion of the mitochondrial gene coding for cytochrome oxidase subunit II and in the 16S rRNA gene. Following PCR amplification, fragments of three sizes were detected. The M. incognita and M. javanica reactions produced a 1.7-kb fragment; the M. arenaria reaction, a 1.1-kb fragment; and the …
Mitochondrial Dna Sequence Divergence Among Meloidogyne Incognita, Romanomermis Culicivorax, Ascaris Suum, And Caenorhabditis Elegans, Thomas O. Powers, T. S. Harris, B. C. Hyman
Mitochondrial Dna Sequence Divergence Among Meloidogyne Incognita, Romanomermis Culicivorax, Ascaris Suum, And Caenorhabditis Elegans, Thomas O. Powers, T. S. Harris, B. C. Hyman
Department of Plant Pathology: Faculty Publications
Mitochondrial DNA sequences were obtained from the NADH dehydrogenase subunit 3 (ND3), large rRNA, and cytochrome b genes from Meloidogyne incognita and Romanomermis culicivorax. Both species show considerable genetic distance within these same genes when compared with Caenorhabditis elegans or Ascaris suum, two species previously analyzed. Caenorhabditis, Ascaris, and Meloidogyne were selected as representatives of three subclasses in the nematode class Secernentea: Rhabditia, Spiruria, and Diplogasteria, respectively. Romanomermis served as a representative outgroup of the class Adenophorea. The divergence between the phytoparasitic lineage (represented by Meloidogyne) and the three other species is so great that virtually …