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Articles 1 - 30 of 205
Full-Text Articles in Life Sciences
A Shared Gene Expression Signature In Mouse Models Of Ebv-Associated And Non-Ebv-Associated Burkitt Lymphoma, Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada, Richard Longnecker
A Shared Gene Expression Signature In Mouse Models Of Ebv-Associated And Non-Ebv-Associated Burkitt Lymphoma, Kathryn T. Bieging, Kamonwan Fish, Subbarao Bondada, Richard Longnecker
Microbiology, Immunology, and Molecular Genetics Faculty Publications
The link between EBV infection and Burkitt lymphoma (BL) is strong, but the mechanism underlying that link has been elusive. We have developed a mouse model for EBV-associated BL in which LMP2A, an EBV latency protein, and MYC are expressed in B cells. Our model has demonstrated the ability of LMP2A to accelerate tumor onset, increase spleen size, and bypass p53 inactivation. Here we describe the results of total gene expression analysis of tumor and pretumor B cells from our transgenic mouse model. Although we see many phenotypic differences and changes in gene expression in pretumor B cells, the transcriptional …
Synergistic Roles Of Eukaryotic Translation Elongation Factors 1bγ And 1a In Stimulation Of Tombusvirus Minus-Strand Synthesis, Zsuzsanna Sasvari, Lara Izotova, Terri Goss Kinzy, Peter D. Nagy
Synergistic Roles Of Eukaryotic Translation Elongation Factors 1bγ And 1a In Stimulation Of Tombusvirus Minus-Strand Synthesis, Zsuzsanna Sasvari, Lara Izotova, Terri Goss Kinzy, Peter D. Nagy
Plant Pathology Faculty Publications
Host factors are recruited into viral replicase complexes to aid replication of plus-strand RNA viruses. In this paper, we show that deletion of eukaryotic translation elongation factor 1Bgamma (eEF1Bγ) reduces Tomato bushy stunt virus (TBSV) replication in yeast host. Also, knock down of eEF1Bγ level in plant host decreases TBSV accumulation. eEF1Bγ binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex. Additional in vitro assays with whole cell extracts prepared from yeast strains lacking eEF1Bγ demonstrated its role in minus-strand synthesis by opening of the structured 3' end of the viral …
Juvenile Hormone Regulates Vitellogenin Gene Expression Through Insulin-Like Peptide Signaling Pathway In The Red Flour Beetle, Tribolium Castaneum, Zhentao Sheng, Jingjing Xu, Hua Bai, Fang Zhu, Subba R. Palli
Juvenile Hormone Regulates Vitellogenin Gene Expression Through Insulin-Like Peptide Signaling Pathway In The Red Flour Beetle, Tribolium Castaneum, Zhentao Sheng, Jingjing Xu, Hua Bai, Fang Zhu, Subba R. Palli
Entomology Faculty Publications
Our recent studies identified juvenile hormone (JH) and nutrition as the two key signals that regulate vitellogenin (Vg) gene expression in the red flour beetle, Tribolium castaneum. Juvenile hormone regulation of Vg synthesis has been known for a long time in several insects, but the mechanism of JH action is not known. Experiments were conducted to determine the mechanism of action of these two signals in regulation of Vg gene expression. Injection of bovine insulin or FOXO double-stranded RNA into the previtellogenic, starved, or JH-deficient female adults increased Vg mRNA and protein levels, thereby implicating the pivotal role for …
Maternal Separation Affects Dopamine Transporter Function In The Spontaneously Hypertensive Rat: An In Vivo Electrochemical Study, Jacqueline S. Womersley, Jennifer H. Hsieh, Lauriston A. Kellaway, Greg A. Gerhardt, Vivienne A. Russell
Maternal Separation Affects Dopamine Transporter Function In The Spontaneously Hypertensive Rat: An In Vivo Electrochemical Study, Jacqueline S. Womersley, Jennifer H. Hsieh, Lauriston A. Kellaway, Greg A. Gerhardt, Vivienne A. Russell
Neuroscience Faculty Publications
BACKGROUND: Attention-deficit/hyperactivity disorder (ADHD) is a developmental disorder characterised by symptoms of inattention, impulsivity and hyperactivity. The spontaneously hypertensive rat (SHR) is a well-characterised model of this disorder and has been shown to exhibit dopamine dysregulation, one of the hypothesised causes of ADHD. Since stress experienced in the early stages of life can have long-lasting effects on behaviour, it was considered that early life stress may alter development of the dopaminergic system and thereby contribute to the behavioural characteristics of SHR. It was hypothesized that maternal separation would alter dopamine regulation by the transporter (DAT) in ways that distinguish SHR …
A Comparison Of Intensive Care Unit Mortality Prediction Models Through The Use Of Data Mining Techniques, Sujin Kim, Woojae Kim, Rae Woong Park
A Comparison Of Intensive Care Unit Mortality Prediction Models Through The Use Of Data Mining Techniques, Sujin Kim, Woojae Kim, Rae Woong Park
Institute for Biomedical Informatics Faculty Publications
OBJECTIVES: The intensive care environment generates a wealth of critical care data suited to developing a well-calibrated prediction tool. This study was done to develop an intensive care unit (ICU) mortality prediction model built on University of Kentucky Hospital (UKH)'s data and to assess whether the performance of various data mining techniques, such as the artificial neural network (ANN), support vector machine (SVM) and decision trees (DT), outperform the conventional logistic regression (LR) statistical model.
METHODS: The models were built on ICU data collected regarding 38,474 admissions to the UKH between January 1998 and September 2007. The first 24 hours …
Forage News [2011-12], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News [2011-12], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News
- Heart of America Grazing Conference
- UK Forage Variety Tests
- Forage Dynamics and Calf Behavior in Two Creep Grazing Systems
- Testing and Feeding Tobacco-Brown Hay and Silage
- World Population Hits Seven Billion
- Farmers Use of Broadband Internet
- High Cattle Prices to Continue, Analysts Say
Fungal Desaturases And Related Methods, David F. Hildebrand, Suryadevara S. Rao, John Thoguru
Fungal Desaturases And Related Methods, David F. Hildebrand, Suryadevara S. Rao, John Thoguru
Plant and Soil Sciences Faculty Patents
The presently-disclosed subject matter provides isolated nucleic acid and amino acid sequences encoding mushroom desaturase polypeptides that are active with both palmitic and stearic acid, as well as vectors and transgenic plant cells comprising nucleic acids of the presently-disclosed subject matter. The presently-disclosed subject matter further provides methods of producing monounsaturated fatty acids, such as palmitoleic acid (16:1), and monounsaturated fatty acids prepared by the methods disclosed herein.
Age-Associated Disruption Of Molecular Clock Expression In Skeletal Muscle Of The Spontaneously Hypertensive Rat, Mitsunori Miyazaki, Elizabeth Schroder, Stephanie E. Edelmann, Michael E. Hughes, Karl Kornacker, C. William Balke, Karyn A. Esser
Age-Associated Disruption Of Molecular Clock Expression In Skeletal Muscle Of The Spontaneously Hypertensive Rat, Mitsunori Miyazaki, Elizabeth Schroder, Stephanie E. Edelmann, Michael E. Hughes, Karl Kornacker, C. William Balke, Karyn A. Esser
Physiology Faculty Publications
It is well known that spontaneously hypertensive rats (SHR) develop muscle pathologies with hypertension and heart failure, though the mechanism remains poorly understood. Woon et al. (2007) linked the circadian clock gene Bmal1 to hypertension and metabolic dysfunction in the SHR. Building on these findings, we compared the expression pattern of several core-clock genes in the gastrocnemius muscle of aged SHR (80 weeks; overt heart failure) compared to aged-matched control WKY strain. Heart failure was associated with marked effects on the expression of Bmal1, Clock and Rora in addition to several non-circadian genes important in regulating skeletal muscle phenotype including …
Investigating A Conformational Change In The Enzyme Neurolysin, Fei Xiong
Investigating A Conformational Change In The Enzyme Neurolysin, Fei Xiong
Kaleidoscope
No abstract provided.
Is The Common Teasel (Dipsacus Sylvestris) A Carnivorous Plant Or Was Francis Darwin Wrong?, Justin Matthew Thomas
Is The Common Teasel (Dipsacus Sylvestris) A Carnivorous Plant Or Was Francis Darwin Wrong?, Justin Matthew Thomas
Kaleidoscope
No abstract provided.
Smp1-3 Insight Into Seed Longevity, Taylor Lloyd
An Exotic Invader Threatens An Ecologically Sensitive Bluegrass Savanna Remnant, R. Murphy Coy
An Exotic Invader Threatens An Ecologically Sensitive Bluegrass Savanna Remnant, R. Murphy Coy
Kaleidoscope
No abstract provided.
Sag101 Forms A Ternary Complex With Eds1 And Pad4 And Is Required For Resistance Signaling Against Turnip Crinkle Virus, Shifeng Zhu, Rae-Dong Jeong, Srivathsa C. Venugopal, Ludmila Lapchyk, Duroy Navarre, Aardra Kachroo, Pradeep Kachroo
Sag101 Forms A Ternary Complex With Eds1 And Pad4 And Is Required For Resistance Signaling Against Turnip Crinkle Virus, Shifeng Zhu, Rae-Dong Jeong, Srivathsa C. Venugopal, Ludmila Lapchyk, Duroy Navarre, Aardra Kachroo, Pradeep Kachroo
Plant Pathology Faculty Publications
EDS1, PAD4, and SAG101 are common regulators of plant immunity against many pathogens. EDS1 interacts with both PAD4 and SAG101 but direct interaction between PAD4 and SAG101 has not been detected, leading to the suggestion that the EDS1-PAD4 and EDS1-SAG101 complexes are distinct. We show that EDS1, PAD4, and SAG101 are present in a single complex in planta. While this complex is preferentially nuclear localized, it can be redirected to the cytoplasm in the presence of an extranuclear form of EDS1. PAD4 and SAG101 can in turn, regulate the subcellular localization of EDS1. We also show that the Arabidopsis genome …
Investigating Calmodulin-Long Qt Syndrome Restorative Interactions Through Combinatorial Approaches, Michael Bricken
Investigating Calmodulin-Long Qt Syndrome Restorative Interactions Through Combinatorial Approaches, Michael Bricken
Kaleidoscope
No abstract provided.
Heart And Ventilatory Measures In Crayfish During Copulation, Richard M. Cooper, Heidi Schapker Finucane, Megan Adami, Robin L. Cooper
Heart And Ventilatory Measures In Crayfish During Copulation, Richard M. Cooper, Heidi Schapker Finucane, Megan Adami, Robin L. Cooper
Biology Faculty Publications
Monitoring heart rate (HR) and ventilatory rate (VR) during defined sensory stimuli and during aggressive and submissive social interactions has provided additional information of a crayfish's physiological state which is not achieved by behavioral observations. In this study, the HR and VR of crayfish were monitored before, during and after the act of copulation in both heterosexual partners. The female crayfish abruptly reduces HR and VR during copulation but the male maintains HR and VR. After separation from copulation the female HR and VR are elevated, potentially paying back the O2 debt. The tight relationship with HR and VR …
Forage News [2011-11], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News [2011-11], Department Of Plant And Soil Sciences, University Of Kentucky
Forage News
- Brent White is Kentucky Forage Spokesman
- KFGC Presents Awards
- KFGC Nominating Committee Report
- AFGC Coming to Louisville
- Forages at KCA
- Selling & Shipping Hay to Texas and Other Southern Tier States
- Hay Production Changes in Kentucky
- Climate Change Effects on Forage Quality of Kentucky Hay Fields
- Be Alert to Health Risks When Grazing Turnips
Revision Of The New World Genus Crassomicrodus Ashmead (Hymenoptera, Braconidae, Agathidinae), With An Identification Key To Species, José Isaac Figueroa, Michael J. Sharkey, Jesus Romero Nápoles, José Antonio Sánchez García, Ana Mabel Martínez, Victor López-Martínez, Samuel Pineda
Revision Of The New World Genus Crassomicrodus Ashmead (Hymenoptera, Braconidae, Agathidinae), With An Identification Key To Species, José Isaac Figueroa, Michael J. Sharkey, Jesus Romero Nápoles, José Antonio Sánchez García, Ana Mabel Martínez, Victor López-Martínez, Samuel Pineda
Entomology Faculty Publications
A key to species and descriptions are presented for 14 species of the New World genus Crassomicrodus Ashmead. Seven new species, Crassomicrodus azteca, Crassomicrodus clypealis, Crassomicrodus costaricensis, Crassomicrodus jalisciensis, Crassomicrodus mariae, Crassomicrodus oaxaquensis,and Crassomicrodus olgae are described. Crassomicrodus fenestratus (Viereck) is synonymized with Crassomicrodus nigriceps (Cresson). Crassomicrodus melanopleurus (Ashmead) is recognized as a valid species.
Reversal Of Glial And Neurovascular Markers Of Unhealthy Brain Aging By Exercise In Middle-Aged Female Mice, Caitlin S. Latimer, James L. Searcy, Michael T. Bridges, Lawrence D. Brewer, Jelena Popović, Eric M. Blalock, Philip W. Landfield, Olivier Thibault, Nada M. Porter
Reversal Of Glial And Neurovascular Markers Of Unhealthy Brain Aging By Exercise In Middle-Aged Female Mice, Caitlin S. Latimer, James L. Searcy, Michael T. Bridges, Lawrence D. Brewer, Jelena Popović, Eric M. Blalock, Philip W. Landfield, Olivier Thibault, Nada M. Porter
Pharmacology and Nutritional Sciences Faculty Publications
Healthy brain aging and cognitive function are promoted by exercise. The benefits of exercise are attributed to several mechanisms, many which highlight its neuroprotective role via actions that enhance neurogenesis, neuronal morphology and/or neurotrophin release. However, the brain is also composed of glial and vascular elements, and comparatively less is known regarding the effects of exercise on these components in the aging brain. Here, we show that aerobic exercise at mid-age decreased markers of unhealthy brain aging including astrocyte hypertrophy, a hallmark of brain aging. Middle-aged female mice were assigned to a sedentary group or provided a running wheel for …
Thrombospondin1 Deficiency Reduces Obesity-Associated Inflammation And Improves Insulin Sensitivity In A Diet-Induced Obese Mouse Model, Yanzhang Li, Xiaopeng Tong, Courtney Rumala, Kate Clemons, Shuxia Wang
Thrombospondin1 Deficiency Reduces Obesity-Associated Inflammation And Improves Insulin Sensitivity In A Diet-Induced Obese Mouse Model, Yanzhang Li, Xiaopeng Tong, Courtney Rumala, Kate Clemons, Shuxia Wang
Graduate Center for Nutritional Sciences Faculty Publications
BACKGROUND: Obesity is prevalent worldwide and is associated with insulin resistance. Advanced studies suggest that obesity-associated low-grade chronic inflammation contributes to the development of insulin resistance and other metabolic complications. Thrombospondin 1 (TSP1) is a multifunctional extracellular matrix protein that is up-regulated in inflamed adipose tissue. A recent study suggests a positive correlation of TSP1 with obesity, adipose inflammation, and insulin resistance. However, the direct effect of TSP1 on obesity and insulin resistance is not known. Therefore, we investigated the role of TSP1 in mediating obesity-associated inflammation and insulin resistance by using TSP1 knockout mice.
METHODOLOGY/PRINCIPAL FINDINGS: Male TSP1-/- mice …
Identification And Thermochemical Analysis Of High-Lignin Feedstocks For Biofuel And Biochemical Production, Venugopal Mendu, Anne E. Harman-Ware, Mark Crocker, Jungho Jae, Jozsef Stork, Samuel Morton, Andrew Placido, George Huber, Seth Debolt
Identification And Thermochemical Analysis Of High-Lignin Feedstocks For Biofuel And Biochemical Production, Venugopal Mendu, Anne E. Harman-Ware, Mark Crocker, Jungho Jae, Jozsef Stork, Samuel Morton, Andrew Placido, George Huber, Seth Debolt
Horticulture Faculty Publications
BACKGROUND: Lignin is a highly abundant biopolymer synthesized by plants as a complex component of plant secondary cell walls. Efforts to utilize lignin-based bioproducts are needed.
RESULTS: Herein we identify and characterize the composition and pyrolytic deconstruction characteristics of high-lignin feedstocks. Feedstocks displaying the highest levels of lignin were identified as drupe endocarp biomass arising as agricultural waste from horticultural crops. By performing pyrolysis coupled to gas chromatography-mass spectrometry, we characterized lignin-derived deconstruction products from endocarp biomass and compared these with switchgrass. By comparing individual pyrolytic products, we document higher amounts of acetic acid, 1-hydroxy-2-propanone, acetone and furfural in switchgrass …
A New Genus Of Agathidinae With The Description Of A New Species Parasitic On Samea Multiplicalis (Guenée), Michael J. Sharkey, Katherine A. Parys, Stephanie Clutts
A New Genus Of Agathidinae With The Description Of A New Species Parasitic On Samea Multiplicalis (Guenée), Michael J. Sharkey, Katherine A. Parys, Stephanie Clutts
Entomology Faculty Publications
A new genus of Agathidinae (Hymenoptera, Braconidae), Neothlipsis, is proposed to include 10 species. Nine of these species were formerly included in the polyphyletic genus Therophilus Wesmael 1837, formerly referred to as Bassus. A new species, Neothlipsis parysae, parasitic on Samea multiplicalis (Guenée), is described. The ten described species transferred to Neothlipsis are:, Neothlipsis agathoides, comb. n. for Bassus agathoides Newton and Sharkey 2000; Neothlipsis agilis, comb. n. for Bassus agilis Cresson 1868; Neothlipsis brevicauda, comb. n. for Bassus brevicaudus Muesebeck 1932; Neothlipsis californica, comb. n. for Bassus californicus Muesebeck 1927; Neothlipsis cincta, comb. n. for Microdus …
Anatomical Correlates To Nectar Feeding Among The Strepsirrhines Of Madagascar: Implications For Interpreting The Fossil Record, Magdalena N. Muchlinski, Jonathan M. G. Perry
Anatomical Correlates To Nectar Feeding Among The Strepsirrhines Of Madagascar: Implications For Interpreting The Fossil Record, Magdalena N. Muchlinski, Jonathan M. G. Perry
Neuroscience Faculty Publications
One possible ecological scenario for the origin of primates is the archaic pollination and coevolution hypothesis. Its proponents contend that the consumption of nectar by some early primates and the resulting cross-pollination is an example of coevolution that drove adaptive radiations in some primates. This hypothesis is perhaps ecologically sound, but it lacks the morphology-behavior links that would allow us to test it using the fossil record. Here we attempt to identify cranial adaptations to nectar feeding among the strepsirrhines of Madagascar in order to provide such links. Many Malagasy strepsirrhines are considered effective cross-pollinators of the flowers they feed …
Taking “Grazing” To The Next Level, Edward N. Ballard
Taking “Grazing” To The Next Level, Edward N. Ballard
Kentucky Grazing Conference
Since the late 1980’s, producer awareness of pasture management has been increasing. A number of factors have brought this about. Mainly, economic conditions have forced producers to look at their bottom line and this has reflected the low returns from mismanagement of forages and pastures. Also, advancements in the technology of pasture management and improvement have made more intensive utilization of the forage resource more economically viable. Advances in equipment, especially fencing and water equipment, have also helped bring about the increased interest in better grassland management.
My Grazing Experience: Reflections And Observations, Russell C. Hackley
My Grazing Experience: Reflections And Observations, Russell C. Hackley
Kentucky Grazing Conference
My pastures are utilized by a beef operation consisting primarily of a small cow/calf herd (30 cows), and a stocker operation numbering from 300-350 head annually, which are grazed only, from spring until fall. These stockers are purchased, continental breed calves, weighing from 500-550 pounds. In the fall, they are sold to Laura’s Lean Beef, usually at a forward contracted price after adding 300 pounds of gain. Occasionally, the stocking includes calves not eligible for Laura’s Lean which are then sold in truckload lots through internet sales.
Options For Getting Water In Every Paddock, Kevin Laurent
Options For Getting Water In Every Paddock, Kevin Laurent
Kentucky Grazing Conference
Whether you call it rotational grazing, intensive grazing or management intensive grazing, the economic benefits of controlling how and where your cattle graze are well documented. Increased forage utilization, greater stocking rates, greater legume persistence, reduced hay feeding and more uniform nutrient recycling are just some of the many benefits producers can take advantage of when practicing some form of controlled grazing. However, one of the greatest challenges to implementing a controlled grazing system is the delivery of stock water to the grazing animal.
Stockpiling Tall Fescue: Cost & Return, Greg S. Halich
Stockpiling Tall Fescue: Cost & Return, Greg S. Halich
Kentucky Grazing Conference
An opportunity that Kentucky cattle farmers have in reducing their hay requirements is to apply nitrogen on select pastures to stockpile for fall and winter grazing. By increasing the total pasture production during this time period, the grazing season can be extended and the amount of hay required can be reduced.
Ryzup Smartgrass: Growth Promotion For Forages, S. Ray Smith
Ryzup Smartgrass: Growth Promotion For Forages, S. Ray Smith
Kentucky Grazing Conference
Whenever we discuss grazing management we emphasize how proper grazing will produce the maximum amount of plant growth. Giving forage plants an adequate rest period after each grazing event is essential to allow growth and storage of carbohydrates for the next growth cycle. With grasses, leaving leaf area after grazing allows the plant to maintain photosynthesis and regrow faster. On a more basic level though, all plants regulate growth with hormones. These hormones are termed “plant growth regulators.” Auxin controls cell division and the direction of plant growth. Gibberellic acid (abbrev. GA) has three main functions in plants: 1) stimulate …
Benefits Of "Improved" Grazing--More Important Now Than Ever!, Garry D. Lacefield
Benefits Of "Improved" Grazing--More Important Now Than Ever!, Garry D. Lacefield
Kentucky Grazing Conference
At this Conference twelve years ago I talked about the “Benefits of Improved Grazing”. We have had aspects of that theme at every Kentucky Grazing Conference since and also emphasized that message at 10 Heart of America Grazing Conferences and three National Grazing Conferences. With all that emphasis, why do I bring this up again and even have the nerve to say “More Important Now than Ever”? Well, the short answer is “things are different now!”
Foreword And Kfgc Award Winners [2011], Garry D. Lacefield, Christi L. Forsythe
Foreword And Kfgc Award Winners [2011], Garry D. Lacefield, Christi L. Forsythe
Kentucky Grazing Conference
No abstract provided.
Wolbachia Infections That Reduce Immature Insect Survival: Predicted Impacts On Population Replacement, Philip R. Crain, James W. Mains, Eunho Suh, Yunxin Huang, Philip H. Crowley, Stephen L. Dobson
Wolbachia Infections That Reduce Immature Insect Survival: Predicted Impacts On Population Replacement, Philip R. Crain, James W. Mains, Eunho Suh, Yunxin Huang, Philip H. Crowley, Stephen L. Dobson
Entomology Faculty Publications
BACKGROUND: The evolutionary success of Wolbachia bacteria, infections of which are widespread in invertebrates, is largely attributed to an ability to manipulate host reproduction without imposing substantial fitness costs. Here, we describe a stage-structured model with deterministic immature lifestages and a stochastic adult female lifestage. Simulations were conducted to better understand Wolbachia invasions into uninfected host populations. The model includes conventional Wolbachia parameters (the level of cytoplasmic incompatibility, maternal inheritance, the relative fecundity of infected females, and the initial Wolbachia infection frequency) and a new parameter termed relative larval viability (RLV), which is the survival of infected larvae relative to …