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Articles 271 - 298 of 298
Full-Text Articles in Life Sciences
The Effects Of Vitamin E On The Antioxidant System, Egg Production, And Egg Quality In Heat Stressed Laying Hens, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
The Effects Of Vitamin E On The Antioxidant System, Egg Production, And Egg Quality In Heat Stressed Laying Hens, Hasret Yardi̇bi̇, Gülhan Türkay Hoştürk
Turkish Journal of Veterinary & Animal Sciences
An experiment was carried out to investigate the effect of vitamin E on the metabolic impact of heat stress in hens. The study included 150 Leghorn laying hens, which were assigned to 2 groups and initially subjected to the same environmental conditions. Diets were based on standard layer rations with 30, 80, or 105 mg of vitamin E/kg. All birds were kept in 45% relative humidity (RH) and at a room temperature of 21 °C for first 3 weeks for adaptation. In the fourth week the temperature and RH were increased to 35 °C and 65%, respectively, in the experimental …
Yeasts As Ripening Adjunct Cultures In Turkish White Brined Cheese Production, Harun Kesenkaş, Necati̇ Akbulut
Yeasts As Ripening Adjunct Cultures In Turkish White Brined Cheese Production, Harun Kesenkaş, Necati̇ Akbulut
Turkish Journal of Veterinary & Animal Sciences
The present study aimed to determine the possibility of using yeasts as adjunct cultures in the production of white brined cheese, which is quite important to the Turkish dairy industry, and to evaluate the effects of these yeasts on cheese ripening. In addition to starter culture (Lactococcus lactis subsp. lactis + Lactococcus lactis subsp. cremoris), adjunct yeast cultures of Yarrowia lipolytica, Debaryomyces hansenii, and Kluyveromyces marxianus were separately co-inoculated to each cheese vat and, therefore, 4 different experimental cheese samples were produced, including the control cheese. Some chemical, microbiological, and sensory properties of the samples were monitored during the 90-day …
Prediction Of Postpubertal Reproductive Potential According To Prepubertal Body Weight, Testicular Size, And Testosterone Concentration Using Multiple Regression Analysis In Kıvırcık Ram Lambs, Özkan Elmaz, Serdal Di̇kmen, Ümi̇t Ci̇ri̇t, Hidir Demi̇r
Prediction Of Postpubertal Reproductive Potential According To Prepubertal Body Weight, Testicular Size, And Testosterone Concentration Using Multiple Regression Analysis In Kıvırcık Ram Lambs, Özkan Elmaz, Serdal Di̇kmen, Ümi̇t Ci̇ri̇t, Hidir Demi̇r
Turkish Journal of Veterinary & Animal Sciences
The relationship between the prepubertal body weight, testicular size, testosterone concentration, and postpubertal reproductive function was investigated in Kıvırcık ram lambs. The body weight, testicular size, and testosterone concentration were measured every 20 days between 60 and 420 days of age. Semen was collected from the ram lambs at 7, 8, 9, 10, 11, 12, 13 and 14 months of age. Data obtained were analyzed by best subsets regression model. We determined that body weight, scrotal circumference, testicular length, and testosterone levels in the early prepubertal period (days 80, 100, and 120), when combined with each other, allow the determination …
Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu
Surfactant Proteins A And D In The Seminal Plasma Of Stallions, Ayhan Ata, Orhan Kankavi̇, M.Aki̇f Çi̇ftçi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
We have previously shown that surfactant protein (SP)-A and SP-D are present in the stallion´s genital system. The aim of the present study was to evaluate the expression and presence of SP-A and SP-D in the seminal plasma and accessory sex glands of stallions. We characterized further the SP-A and SP-D in the seminal plasma of stallions by western blotting and immunohistochemically in accessory sex glands. On one-dimensional SDS-PAGE electrophoresis and western blots, bands for SP-A (34 kDa) and SP-D (43 kDa) in seminal plasma and accessory sex gland tissue extracts were detected. The similarity between biochemical properties (relative molecular …
An Evaluation Of Subclinical Mastitis During Lactation In Anatolian Buffaloes, Erhan Özenç, Mehmet Rifat Vural, Esra Şeker, Mehmet Uçar
An Evaluation Of Subclinical Mastitis During Lactation In Anatolian Buffaloes, Erhan Özenç, Mehmet Rifat Vural, Esra Şeker, Mehmet Uçar
Turkish Journal of Veterinary & Animal Sciences
Subclinical mastitis in Anatolian buffaloes (n = 71) in Afyon, Turkey was evaluated using 1637 milk samples collected monthly for 9 months. Tests applied to milk samples included the California Mastitis Test (CMT), somatic cell count (SCC), and bacteriological examination. The threshold limit for SCC was found as 130 × 10^3 cells/ml. Anatolian buffaloes are infected above this level and they are likely to be uninfected below this level. The highest rates of intramammary infection (IMI) per quarter (36.5%) and animal (69.1%) were encountered during the highest rainfall month of May. Candida spp., coagulase-negative staphylococcus (CNS) and Staphylococcus aureus and …
The Effects Of Seminal Plasma, Skim Milk, And Tyrodes Solutions On Survival Of Stallion Sperm Stored At 4 °C, Ergun Akçay
The Effects Of Seminal Plasma, Skim Milk, And Tyrodes Solutions On Survival Of Stallion Sperm Stored At 4 °C, Ergun Akçay
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to evaluate the effects of skim milk, skim milk extender supplemented with modified Tyrode´s medium, and seminal plasma (obtained from the sperm-rich fraction) on stallion sperm survival after 24 h cooled storage at 4 °C. Ejaculates from 3 Finnhorse stallions were used in this study. Ejaculates were collected by an automated phantom (Equidame) which separates fractions of semen into 5 cups. Only the second cup (sperm-rich fraction) was used to obtain spermatozoa and seminal plasma for the experiment. Semen was diluted with the stallion´s own second cup seminal plasma and the extenders to a final concentration …
Ultrasonographic Examination Of The Goat Liver, Sarang Soroori, Afshin Raoofi, Ali Reza Vajhi, Sara Ghorbani Nezami
Ultrasonographic Examination Of The Goat Liver, Sarang Soroori, Afshin Raoofi, Ali Reza Vajhi, Sara Ghorbani Nezami
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to obtain detailed information about the normal size and texture of the goat liver by means of ultrasonographic examination. The structure, location, and size of the liver, portal vein, and caudal vena cava were examined ultrasonographically in 6 goats. The angle of the liver and the thickness of the gallbladder wall were also determined. Examinations were performed on the right side of the abdomen, from the 6th through 12th intercostal space, using a 6-13 MHz linear transducer. In each intercostal space the dimensions of the liver, and if visible, the location and diameter of …
Investigation Of Reproductive Traits And A Computer-Assisted Single Artificial Insemination Procedure In Mares In Subtropical Taiwan, Jacky Peng-Wen Chan, Jui-Te Wu, Feng-Pang Cheng, Shi-Te Chuang, Chi-Chung Chou
Investigation Of Reproductive Traits And A Computer-Assisted Single Artificial Insemination Procedure In Mares In Subtropical Taiwan, Jacky Peng-Wen Chan, Jui-Te Wu, Feng-Pang Cheng, Shi-Te Chuang, Chi-Chung Chou
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to report the reproductive traits of mares by ultrasonography and hormone analyses in subtropical Taiwan and the successful artificial breeding of mares using a self-developed computer-assisted single insemination technique. A previously developed computer-assisted image analysis technique at our reproduction centre was employed to determine the optimal time for artificial insemination (AI). In the 8 mares that underwent this AI procedure, ovulations all occurred within 12 h post-insemination and 5 mares were impregnated (62.5%). The combined results of ultrasonic examination and hormone analyses showed that the mares in Taiwan were not only possible defined seasonal …
Iatrogenic Hyperlipidemia Associated With Lipid-Laden Aqueous Humor In A Cat Following Administration Of Megestrol Acetate, Masoud Selk Ghaffari
Iatrogenic Hyperlipidemia Associated With Lipid-Laden Aqueous Humor In A Cat Following Administration Of Megestrol Acetate, Masoud Selk Ghaffari
Turkish Journal of Veterinary & Animal Sciences
A 2-year-old male cat presented with sudden onset of binocular blindness in both eyes. The appearance of eyes seemed to change to cloudy white. Evaluation of intraocular components of the anterior segment was completely impossible due to the presence of homogeneous discoloration of the affected eyes. Careful history taking revealed that the cat had received megestrol acetate prescribed by the referring veterinarian for the treatment of urine spraying for 10 months. Laboratory findings revealed hypertriglyceridemia and hypercholesterolemia. According to the history of drug therapy, ocular signs, and laboratory findings, a diagnosis of drug induced lipemia and subsequent lipid-laden aqueous humor …
Estimation Of The Nitrogen-Phosphorus Load Caused By Rainbow Trout (Oncorhynchus Mykiss Walbaum, 1792) Cage-Culture Farms In Kesikköprü Dam Lake: A Comparison Of Pelleted And Extruded Feed, Umut Aşir, Serap Pulatsü
Estimation Of The Nitrogen-Phosphorus Load Caused By Rainbow Trout (Oncorhynchus Mykiss Walbaum, 1792) Cage-Culture Farms In Kesikköprü Dam Lake: A Comparison Of Pelleted And Extruded Feed, Umut Aşir, Serap Pulatsü
Turkish Journal of Veterinary & Animal Sciences
This research was conducted in order to estimate the nitrogen and phosphorus loads released into Kesikköprü Dam Lake between April and July 2006 from 2 different rainbow trout (Oncorhynchus mykiss Walbaum, 1792) cage farms. Both farms had a capacity of about 20 t year^{-1}, and used pelleted and extruded feed. In Kesikköprü Dam Lake the nitrogen load was estimated to be between 56.00 and 62.92 kg t^{-1}of fish produced, and the phosphorus load was estimated to be between 10.66 and 12.17 kg t^{-1}of fish produced in cages in which pelleted feed was used, In cages in which extruded feed was …
Antagonism Of Medetomidine Sedation In Goats By Atipamezole And Yohimbine, Mahmood B. Mahmood, Fouad K. Mohammad
Antagonism Of Medetomidine Sedation In Goats By Atipamezole And Yohimbine, Mahmood B. Mahmood, Fouad K. Mohammad
Turkish Journal of Veterinary & Animal Sciences
The alpha 2-adrenoceptor antagonists atipamezole and yohimbine were used to antagonize medetomidine-induced sedation in 6-8-month-old native breed female goats. Intramuscular injection of medetomidine at the dose rate of 40 \mu g/kg body weight induced sedation and recumbency within 10-20 min in the goats. After recumbency, the goats were injected with saline (5 ml) (control group), atipamezole (0.2 mg/kg), or yohimbine (0.2 mg/kg) via the jugular vein. The duration of recumbency in the control group was 90 ± 9 min (mean ± SE). The effects of sedation continued in the control group goats even after regaining the righting reflex and standing. …
Production Losses Due To Endemic Foot-And-Mouth Disease In Cattle In Turkey, Berri̇n Şentürk, Cengi̇z Yalçin
Production Losses Due To Endemic Foot-And-Mouth Disease In Cattle In Turkey, Berri̇n Şentürk, Cengi̇z Yalçin
Turkish Journal of Veterinary & Animal Sciences
This research paper is aimed at estimating foot-and-mouth disease (FMD)-induced production losses in individual dairy and fattening cattle in Turkey. The data used were obtained from a Delphi Expert Opinion Survey. The weighted average FMD-induced financial losses for all breeds were estimated to be $294/head for a milking cow, $152/head for a dairy heifer and $197/head for beef cattle. However, the amount of financial loss varied across different breeds, ages, and sexes. The study findings suggest that culling Holstein breed cows, beef cattle, and Holstein and cross breed female calves, as soon as they have an FMD infection, would be …
The Effect Of Duration Of Finishing Period On The Performance, Slaughter, Carcass, And Beef Quality Characteristics Of Eastern Anatolian Red Bulls, Abdülkadi̇r Özlütürk, Nuri̇ni̇sa Esenbuğa, Mete Yanar, Necdet Ünlü, Muhli̇s Maci̇t, Si̇nan Kopuzlu
The Effect Of Duration Of Finishing Period On The Performance, Slaughter, Carcass, And Beef Quality Characteristics Of Eastern Anatolian Red Bulls, Abdülkadi̇r Özlütürk, Nuri̇ni̇sa Esenbuğa, Mete Yanar, Necdet Ünlü, Muhli̇s Maci̇t, Si̇nan Kopuzlu
Turkish Journal of Veterinary & Animal Sciences
Seventeen Eastern Anatolian Red (EAR) bulls at 20 months of age were divided into 2 treatment groups and fed for either 168 days (S) or 210 days (L). Initial and final weights were not significantly different. While bulls in the L group had the highest total weight gain (149.0 vs. 177.2 kg), feed efficiency ratio was superior for the cattle fed for 168 days. Carcass weight, spleen and front + hind feet weights as a percentage of slaughter weight, weight of kidney fat, percentage of kidney, pelvic, and heart fat, and yield grade all increased (P < 0.05) with feeding time. Bulls in the L group received a higher (P < 0.05) panel rating for juiciness, and the panel juiciness score was closely associated with tenderness measurements such as panel tenderness score, Warner Bratzler shear force value, and number of chews before swallowing. It is concluded that duration of finishing significantly influenced carcass, slaughter, and organoleptic beef quality traits of young EAR bulls.
Determination Of The Feeding Values Of Feedstuffs And Mixed Feeds Used In The Southeastern Anatolia Region Of Turkey, Murat Sedat Baran, Ramazan Demi̇rel, Di̇lek Şentürk Demi̇rel, Tarkan Şahi̇n, Derya Yeşi̇lbağ
Determination Of The Feeding Values Of Feedstuffs And Mixed Feeds Used In The Southeastern Anatolia Region Of Turkey, Murat Sedat Baran, Ramazan Demi̇rel, Di̇lek Şentürk Demi̇rel, Tarkan Şahi̇n, Derya Yeşi̇lbağ
Turkish Journal of Veterinary & Animal Sciences
It is very important to know the feeding value and metabolizable energy content of feedstuffs for balancing animal diets. Feeding value and energy content of animal feeds change according to maturity stage, soil conditions, fertilization, climate, processing methods, etc. There are no adequate tables that show the basic feeding values of feedstuffs grown in different regions of Turkey; therefore, the present study analyzed 8 different feedstuffs and 56 dairy and beef cattle mixed feeds to determine their feeding value and energy content. Additionally, the possibility of using these feedstuffs for ruminant nutrition is discussed. Crude protein content of the dairy …
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
The Influence Of Epidermal Growth Factor On Maturation And Fertilisation Of Bovine Oocytes In Vitro, Bülent Polat, M. Rifat Salmanoğlu
Turkish Journal of Veterinary & Animal Sciences
The aim of the present study was to evaluate the efficiency of different doses of epidermal growth factor (EGF) on maturation and fertilisation of bovine oocytes in vitro. A total of 1209 high quality oocytes (follicles 2-8 mm in diameter, with unexpanded cumulus cells) were obtained by aspiration from ovaries collected at a slaughterhouse. The oocytes were then assigned into 6 groups: 1, 10, 100, and 1000 ng/ml EGF groups, as well as positive (EGF replaced with hCG) and negative (TCM 199 only, no supplement) control groups. Maturation and fertilisation rates were calculated at 39 °C in an atmosphere of …
Immunomodulatory And Protective Effects Of Sugar Cane Juice In Chickens Against Eimeria Infection, Masood Akhtar, M. Abdul Hafeez, Faqir Muhammad, Ahsan Ul Haq, M. Irfan Anwar
Immunomodulatory And Protective Effects Of Sugar Cane Juice In Chickens Against Eimeria Infection, Masood Akhtar, M. Abdul Hafeez, Faqir Muhammad, Ahsan Ul Haq, M. Irfan Anwar
Turkish Journal of Veterinary & Animal Sciences
Present paper reports the effects of sugar cane juice (SCJ) on immune responses and its protection against Eimeria (mixed species) infections in broiler chickens. Experiment 1 was conducted to test the dose efficacy of SCJ and its effects on chickens against coccidiosis. Maximum weight gain per day was recorded in chickens given SCJ @ 400 mg/kg body weight. Significantly (P < 0.01) low mortality and increased body weight gain per day were recorded in chickens administered SCJ orally and challenged with mixed species of genus Eimeria. Furthermore, mild hemorrhages, lower lesion scores (+1), and significantly lower number of oocysts shed in faeces were recorded in immunized chickens compared to control. Experiment 2 was carried out to evaluate the immunomodulatory effects of SCJ on cellular and humoral responses. Highest antibody titres (1:1024-1:16; GMT 367.28) were recorded in chickens administered SCJ @ 400 mg/kg body weight in comparison with control (1:16-1:2; GMT 2.82). To demonstrate the cell mediated immunity (CMI), amplitude of toe-web swelling 72 h post avian tuberculin injection was recorded in the experimental and control groups. Maximum swelling (8.13 ± 0.21 mm) was recorded in the experimental group as compared to control (1.04 ± 0.10); indicating higher cell mediated immune response. From these results it was concluded that SCJ elicit a significantly higher cellular and humoral responses compared to control, which may provide protection against Eimeria infections (mixed species) in chickens.
Seroprevalence Of Coxiella Burnetii In Stray Cats In Central Anatolia, Selçuk Kiliç, Tomoyoshi Komiya, Beki̇r Çelebi̇, Ni̇jat Aydin, Junko Saito, Hiroko Toriniwa, Bi̇lge Karatepe, Cahi̇t Babur
Seroprevalence Of Coxiella Burnetii In Stray Cats In Central Anatolia, Selçuk Kiliç, Tomoyoshi Komiya, Beki̇r Çelebi̇, Ni̇jat Aydin, Junko Saito, Hiroko Toriniwa, Bi̇lge Karatepe, Cahi̇t Babur
Turkish Journal of Veterinary & Animal Sciences
Coxiella burnetii causes Q fever (Coxiellosis) in humans and animals worldwide. The present study was carried out to determine the seroprevalence of Q fever among stray cats in 3 providences (Ankara, Niğde, and Kayseri) in Central Anatolia, Turkey. A total of 143 sera from stray cats were examined for the presence of IgG against C. burnetii phase II antigen by indirect fluorescent antibody test (IFAT). Seven out of the 143 (4.9%) stray cats were seropositive for Q fever, with titers of 1:64 to 1:256. Seroprevalences in Ankara, Niğde, and Kayseri provinces were 1.6%, 7.4%, and 8.3%, respectively. This is the …
Disseminated Streptococcus Equi Subsp. Zooepidemicus Infection In A Foal And Associated Mastitis In A Mare, A. Levent Kocabiyik, Esra Büyükcangaz, Ahmet Akkoç, Cüneyt Özakin, İ. Taci̇ Cangül
Disseminated Streptococcus Equi Subsp. Zooepidemicus Infection In A Foal And Associated Mastitis In A Mare, A. Levent Kocabiyik, Esra Büyükcangaz, Ahmet Akkoç, Cüneyt Özakin, İ. Taci̇ Cangül
Turkish Journal of Veterinary & Animal Sciences
A systemic infection with Streptococcus equi subsp. zooepidemicus is described in a 22-day-old female foal for the first time in Turkey. Clinically the foal was in poor condition and had severe pneumonia. Painful swellings of the carpal and tarsal joints were evident. Examination of the dam revealed clinical mastitis. Postmortem histopathological and microbiological examinations of samples from the foal revealed a disseminated infection with S. equi subsp. zooepidemicus. The causative agent was also recovered in pure culture from a milk sample of the mare.
New Alien Species For The Flora Of Romania: Bidens Bipinnata L. (Asteraceae), Culita Sirbu, Adrian Oprea
New Alien Species For The Flora Of Romania: Bidens Bipinnata L. (Asteraceae), Culita Sirbu, Adrian Oprea
Turkish Journal of Botany
This paper presents an alien plant species that has recently migrated into Romania, namely Bidens bipinnata L. For this species, the following information is provided: occurrence in Romania, a short botanical description, general distribution, way of migration, and general aspects regarding its biology, ecology, and invasive character.
A Comparative Study On Colchicine Application Methods In Obtaining Doubled Haploids Of Tobacco (Nicotiana Tabacum L.), Betül Bürün, Ülkü Emi̇roğlu
A Comparative Study On Colchicine Application Methods In Obtaining Doubled Haploids Of Tobacco (Nicotiana Tabacum L.), Betül Bürün, Ülkü Emi̇roğlu
Turkish Journal of Biology
In order to obtain androgenic doubled haploids of tobacco (Nicotiana tabacum cv. Karabağlar 6265) colchicine was applied at 3 different stages of anther culture. Before culture, anthers were treated with 0.4% aqueous solution of colchicine for 0, 2, 4, 6, 8, 10, and 12 h. Culture response of anthers decreased as the treatment duration increased (except 12 h) and the highest diploidization of 29.7% was obtained with 6 h. During culture, when macroscopic embryoids appeared in dehisced anthers, they were wrapt up in sterile cotton saturated with 0.2% colchicine for 3 days and transferred to fresh medium. This application resulted …
Chitinase Production By Serratia Marcescens Gg5, Gursharan Singh, Joginder Ram Sharma, Gurinder Singh Hoondal
Chitinase Production By Serratia Marcescens Gg5, Gursharan Singh, Joginder Ram Sharma, Gurinder Singh Hoondal
Turkish Journal of Biology
Swollen chitin, flake chitin, powder chitin, and mushroom paste were used as substrates for chitinase production by Serratia marcescens GG5 in submerged fermentation. Enzyme production was 0.3 U/ml when the organism was grown in M9 medium supplemented with 0.5% swollen chitin and 0.5% soluble starch. Scanning electron microscopy revealed that Serratia marcescens GG5 digested the chitin flakes by producing chitinase.
Optimization Of Process Parameters For Alkaline Protease Production By Bacillus Licheniformis N-2 And Kinetics Studies In Batch Fermentation, Muhammad Nadeem, Javed Iqbal Qazi, Qurat-Ul-Ain Syed, Shahjahan Baig
Optimization Of Process Parameters For Alkaline Protease Production By Bacillus Licheniformis N-2 And Kinetics Studies In Batch Fermentation, Muhammad Nadeem, Javed Iqbal Qazi, Qurat-Ul-Ain Syed, Shahjahan Baig
Turkish Journal of Biology
Production of alkaline protease from B. licheniformis N-2 was studied in shake flask experiments in growth medium comprised of (g/l): glucose, 10; soybean meal, 10; K_2HPO_4, 5; MgSO_4.7H_2O, 0.5; NaCl, 0.5; CaCl_2.2H_2O, 0.5. Different process parameters, such as fermentation period, initial pH, incubation temperature, agitation speed, and size and age of inoculum, were optimized for the maximum yield of the alkaline protease. Maximum enzyme production (991.04 PU/ml) was obtained after 24 h of incubation at 37 °C in growth medium of pH 10. The optimum inoculum size and age were determined as 2.0% (v/v) and 24 h, respectively. Kinetics of …
The Interaction Effects Of Flooding And Kinetin On Growth Criteria, Chlorophyll Content, And 5-Aminolevulinic Acid Dehydratase Activity In Corn Seedlings, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare
The Interaction Effects Of Flooding And Kinetin On Growth Criteria, Chlorophyll Content, And 5-Aminolevulinic Acid Dehydratase Activity In Corn Seedlings, Rashid Jamei, Reza Heidari, Jalil Khara, Samad Zare
Turkish Journal of Biology
The interaction effects of kinetin and flooding on growth indexes, chlorophyll content, and the activity of 5-aminolevulinic acid dehydratase (ALA-D; EC 4.2.1.24) in seedlings of corn (Zea mays L. cv. 704) were investigated. Samples were taken 48, 96, 144, and 192 h after the start of treatment. Growth criteria (root length, shoot height, and dry weight) were inhibited more in flooded plants than in control plants. The pattern of growth changes was similar to chlorophyll a and b, as well as ALA-D activity. The exogenous application of kinetin (100 ppm) nullified the resulting decrease in growth and chlorophyll, but only …
In Vivo Anti-Ulcerogenic Activity Of Equisetum Telmateia Ehrh. Extracts Used In Turkish Folk Medicine, İlhan Gürbüz, Erdem Yeşi̇lada
In Vivo Anti-Ulcerogenic Activity Of Equisetum Telmateia Ehrh. Extracts Used In Turkish Folk Medicine, İlhan Gürbüz, Erdem Yeşi̇lada
Turkish Journal of Biology
The aerial part of Equisetum telmateia Ehrh. is used against various conditions including stomachache and peptic ulcer in Turkish folk medicine. In order to prove the claimed anti-ulcerogenic potential of the plant, aqueous and methanol extracts were prepared from aerial parts of E. telmateia and investigated for their anti-ulcerogenic effect on ethanol-induced gastric ulcer model in rats. Pharmacological experiments clearly demonstrated that the oral administration of both methanol and aqueous extracts of Equisetum telmateia Ehrh. plant showed significant stomach protection (77.9% and 100% ulcer inhibition, respectively) against the applied model of ulcerogenesis (P < 0.001). Since both extracts were found significantly active, 80% ethanol was preferred for the extraction of the plant material and this crude methanolic extract was further fractionated by successive solvent extractions with chloroform and H_2O saturated n-BuOH to obtain the chloroform and n-BuOH extracts as well as the remaining aqueous extract. The 80% ethanol extract and the solvent extracts were then submitted to pharmacological assay using the same in vivo experimental ulcer model. All the extracts statistically showed potent anti-ulcerogenic effect on this model (P < 0.001). However, the remaining aqueous extract was found prominent (98.2% ulcer inhibition). Consequently, folkloric utilization of E. telmateia on peptic ulcer has been confirmed in the present study by using ethanol-induced experimental ulcer model in rats.
Determination Of Antimicrobial And Antioxidant Activities Of Turkish Endemic Salvia Halophila Hedge, Sevi̇l Albayrak, Ahmet Aksoy, Ergi̇n Hamzaoğlu
Determination Of Antimicrobial And Antioxidant Activities Of Turkish Endemic Salvia Halophila Hedge, Sevi̇l Albayrak, Ahmet Aksoy, Ergi̇n Hamzaoğlu
Turkish Journal of Biology
In this study, the antioxidant activity of ethanol extract of Salvia halophila Hedge (Lamiaceae) was evaluated by 2 antioxidant assays, including phosphomolybdenum reduction and free radical scavenging activity. The ethanol extract showed free radical scavenging activity with IC_{50} of 67.73 \mug/mL in 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The antioxidant activity of the extract was found to be 84.87 ± 0.7 mg ascorbic acid equivalent (AAE)/g extract in phosphomolybdenum assay. In the addition to the antioxidant activity of the extract, the total phenolic content was measured. Total phenolic content of ethanol extract was found to be 8.21 ± 0.0 mg gallic acid equivalent …
Investigation Of The Antimutagenic Potentials Of The Methanol Extract Of Origanum Vulgare L. Subsp. Vulgare In The Eastern Anatolia Region Of Turkey, Tüli̇n Özbek, Medi̇ne Güllüce, Fi̇kretti̇n Şahi̇n, Hakan Özkan, Sevgi̇ Sevsay, Özlem Bariş
Investigation Of The Antimutagenic Potentials Of The Methanol Extract Of Origanum Vulgare L. Subsp. Vulgare In The Eastern Anatolia Region Of Turkey, Tüli̇n Özbek, Medi̇ne Güllüce, Fi̇kretti̇n Şahi̇n, Hakan Özkan, Sevgi̇ Sevsay, Özlem Bariş
Turkish Journal of Biology
The aim of this study was to evaluate the antimutagenic activity of the methanol extract of Origanum vulgare L. subsp. vulgare. Antimutagenic activity was estimated by employing the plate incorporation AMES/Salmonella histidine reversion assay. The base pair substitution tester strain Salmonella typhimurium TA1535 and the frame shift mutagen strain S. typhimurium TA1538 were used against direct acting mutagens - sodium azide (NaN_3), 4-nitro-1-quinoline oxide (4NQO) and the S9-dependent mutagen 2-aminofluorene (2AF). The results suggested that in the absence of S9 metabolic activation, all 3 doses (5, 0.5, and 0.05 \mug/plate) of the plant extract tested caused statistically significant (P < 0.05) antimutagenic activity on TA1535 strain, but not on TA1538 strain. However, in the presence of S9 microsomal fraction, the plant extract exerted moderate antimutagenic activity against the 2AF mutagen and reduced mutant colonies in TA1535 and TA1538 strains. As a result, the methanol extract of Origanum vulgare subsp. vulgare showed antimutagenic effects at 5, 0.5, and 0.05 \mug/plate concentrations. These effects may be explained with the antioxidant activity mechanism, changes in membrane lipids, and permeability of ion channels.
Analysis Of Ki-Ras Exon 2 Gene Mutations In 3-Methylcholanthrene And Butylated Hydroxytoluene-Induced Rat Lung Tissues, Fi̇kri̇ye Polat, Öztürk Özdemi̇r, Şahende Elagöz
Analysis Of Ki-Ras Exon 2 Gene Mutations In 3-Methylcholanthrene And Butylated Hydroxytoluene-Induced Rat Lung Tissues, Fi̇kri̇ye Polat, Öztürk Özdemi̇r, Şahende Elagöz
Turkish Journal of Biology
3-Methylcholanthrene (MCA) is a polycyclic aromatic hydrocarbon and potent carcinogenic agent that is often used in experimental cancer studies. Butylated hydroxytoluene (BHT) has been widely used for many years as an antioxidant to preserve and stabilize the freshness, nutritional value, flavor, and color of foods. The aim of the present study was to investigate the role of the application of MCA and BHT in the development of lung cancer, and to detect any mutation in the Ki-ras gene exon 2. Rats were killed by cervical dislocation 26 weeks after the last BHT injection and lung tissues were surgically removed. Lung …
The Effects Of Micronuclei With Whole Chromosome On Biological Dose Estimation, Funda Si̇bel Pala, Fadi̇me Alkaya, Kiymet Tabakçioğlu, Füsun Tokatli, Cem Uzal, Şule Parlar, Çeti̇n Algüneş
The Effects Of Micronuclei With Whole Chromosome On Biological Dose Estimation, Funda Si̇bel Pala, Fadi̇me Alkaya, Kiymet Tabakçioğlu, Füsun Tokatli, Cem Uzal, Şule Parlar, Çeti̇n Algüneş
Turkish Journal of Biology
The total micronucleus (MN) assay has been used for purposes of biological dosimetry for many years. The variable spontaneous incidence of micronuclei in peripheral blood lymphocytes affects the sensitivity of biological dose estimations at low doses. It has been suggested that this problem could be solved by using the micronuclei-centromere assay. In this study, Co-60 gamma ray dose response curves for micronuclei (MN) and micronuclei without centromeres (MNC-) in the range of 0-5.0 Gy were established using a pancentromeric FISH probe on cultured binucleate lymphocytes from 2 donors. There were no significant inter-donor differences in the dose responses for either …