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Full-Text Articles in Life Sciences

Chemical Synthesis Of Sensitive Dna, Komal Chillar Jan 2024

Chemical Synthesis Of Sensitive Dna, Komal Chillar

Dissertations, Master's Theses and Master's Reports

Over the past decades, researchers have tried various chemical methods to synthesize modified oligodeoxynucleotides (ODNs, i.e. short segments of DNAs). Traditional ODN synthesis methods require strong basic, and nucleophilic conditions for the deprotection and cleavage of the ODN from the solid support. However, the sensitive ODNs containing labile functionalities are vulnerable to such harsh conditions. Sensitive ODNs have a wide range of applications in research and pharmaceuticals. To synthesize sensitive ODNs, researchers devised different strategies but no practical methods have been developed. To overcome these challenges, we developed alkyl Dim alkyl Dmoc technology. This innovative technology uses weakly basic and …


Valorization Of Spent Vetiver Roots For Biochar Generation, Sameer Neve, Dibyendu Sarkar, Manas Warke, Teresa Bandosz, Rupali Datta Jan 2024

Valorization Of Spent Vetiver Roots For Biochar Generation, Sameer Neve, Dibyendu Sarkar, Manas Warke, Teresa Bandosz, Rupali Datta

Michigan Tech Publications, Part 2

Vetiver root is widely used to produce essential oils in the aromatherapy industry. After the extraction of oil, the roots are disposed of as waste. The central objective of this research was to explore the conversion of this waste into a resource using a circular economy framework. To generate biochar, vetiver roots were pyrolyzed at different temperatures (300, 500, and 700 °C) and residence times (30, 60, and 120 min). Analysis showed the root biochar generated at 500 °C and held for 60 min had the highest surface area of 308.15 m2/g and a yield of 53.76%, in addition to …


Lead In Paint-Contaminated Residential Soils With Varying Physicochemical Properties From Three Large Us Cities: Assessment Of Geochemical Forms And Recommendations On Amendments For Immobilization, Hadeer Saleh, Dibyendu Sarkar, Washington Braida, Steve Larson, Rupali Datta Dec 2023

Lead In Paint-Contaminated Residential Soils With Varying Physicochemical Properties From Three Large Us Cities: Assessment Of Geochemical Forms And Recommendations On Amendments For Immobilization, Hadeer Saleh, Dibyendu Sarkar, Washington Braida, Steve Larson, Rupali Datta

Michigan Tech Publications, Part 2

Lead in yard soils has been recognized as the principal source of excess lead absorption among young children. The hazard imposed by soil lead is dependent on the geochemical forms of lead in soils. Soil properties such as pH, soil organic matter, clay, and carbonate content influence the geochemical forms of lead in soil. This study was conducted to investigate the correlation between soil properties and the geochemical speciation of lead in lead paint-contaminated residential soils from three major US cities. A comprehensive field survey was conducted, involving the collection of soils from ten houses in each of the cities: …


Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang Dec 2023

Long Oligodeoxynucleotides: Chemical Synthesis, Isolation Via Catching-By-Polymerization, Verification Via Sequencing, And Gene Expression Demonstration, Yipeng Yin, Reed Arneson, Alexander Apostle, Adikari M.D.N. Eriyagama, Komal Chillar, Emily Burke, Martina Jahfetson, Yinan Yuan, Shiyue Fang

Michigan Tech Publications, Part 2

Long oligodeoxynucleotides (ODNs) are segments of DNAs having over one hundred nucleotides (nt). They are typically assembled using enzymatic methods such as PCR and ligation from shorter 20 to 60 nt ODNs produced by automated de novo chemical synthesis. While these methods have made many projects in areas such as synthetic biology and protein engineering possible, they have various drawbacks. For example, they cannot produce genes and genomes with long repeats and have difficulty to produce sequences containing stable secondary structures. Here, we report a direct de novo chemical synthesis of 400 nt ODNs, and their isolation from the complex …


Monitoring Time Domain Characteristics Of Parkinson's Disease Using 3d Memristive Neuromorphic System, Md Abu Bakr Siddique, Yan Zhang, Hongyu An Dec 2023

Monitoring Time Domain Characteristics Of Parkinson's Disease Using 3d Memristive Neuromorphic System, Md Abu Bakr Siddique, Yan Zhang, Hongyu An

Michigan Tech Publications, Part 2

INTRODUCTION: Parkinson's disease (PD) is a neurodegenerative disorder affecting millions of patients. Closed-Loop Deep Brain Stimulation (CL-DBS) is a therapy that can alleviate the symptoms of PD. The CL-DBS system consists of an electrode sending electrical stimulation signals to a specific region of the brain and a battery-powered stimulator implanted in the chest. The electrical stimuli in CL-DBS systems need to be adjusted in real-time in accordance with the state of PD symptoms. Therefore, fast and precise monitoring of PD symptoms is a critical function for CL-DBS systems. However, the current CL-DBS techniques suffer from high computational demands for real-time …


Engineering Exosomes To Specifically Target The Mitochondria Of Brain Cells, Xin Yan, Xinqian Chen, Zhiying Shan, Lanrong Bi Dec 2023

Engineering Exosomes To Specifically Target The Mitochondria Of Brain Cells, Xin Yan, Xinqian Chen, Zhiying Shan, Lanrong Bi

Michigan Tech Publications, Part 2

Mitochondrial dysfunction is associated with various health conditions, including cardiovascular and neurodegenerative diseases. Mitochondrial-targeting therapy aims to restore or enhance mitochondrial function to treat or alleviate these conditions. Exosomes, small vesicles that cells secrete, containing a variety of biomolecules, are critical in cell-to-cell communication and have been studied as potential therapeutic agents. Exosome-based therapy has the potential to treat both cardiovascular and neurodegenerative diseases. Combining these two approaches involves using exosomes as carriers to transport mitochondrial-targeting agents to dysfunctional or damaged mitochondria within target cells. This article presents a new technique for engineering brain-derived exosomes that target mitochondria and has …


Engineering Exosomes To Specifically Target The Mitochondria Of Brain Cells, Xin Yan, Xinqian Chen, Zhiying Shan, Lanrong Bi Dec 2023

Engineering Exosomes To Specifically Target The Mitochondria Of Brain Cells, Xin Yan, Xinqian Chen, Zhiying Shan, Lanrong Bi

Michigan Tech Publications, Part 2

Mitochondrial dysfunction is associated with various health conditions, including cardiovascular and neurodegenerative diseases. Mitochondrial-targeting therapy aims to restore or enhance mitochondrial function to treat or alleviate these conditions. Exosomes, small vesicles that cells secrete, containing a variety of biomolecules, are critical in cell-to-cell communication and have been studied as potential therapeutic agents. Exosome-based therapy has the potential to treat both cardiovascular and neurodegenerative diseases. Combining these two approaches involves using exosomes as carriers to transport mitochondrial-targeting agents to dysfunctional or damaged mitochondria within target cells. This article presents a new technique for engineering brain-derived exosomes that target mitochondria and has …


Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John Vucetich, Jennifer A. Leonard, Robert K. Wayne Dec 2023

Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John Vucetich, Jennifer A. Leonard, Robert K. Wayne

Michigan Tech Publications, Part 2

Effective population size estimates are critical information needed for evolutionary predictions and conservation decisions. This is particularly true for species with social factors that restrict access to breeding or experience repeated fluctuations in population size across generations. We investigated the genomic estimates of effective population size along with diversity, subdivision, and inbreeding from 162,109 minimally filtered and 81,595 statistically neutral and unlinked SNPs genotyped in 437 grey wolf samples from North America collected between 1986 and 2021. We found genetic structure across North America, represented by three distinct demographic histories of western, central, and eastern regions of the continent. Further, …


The Role Of Governance In Rewilding The United States To Stem The Biodiversity Crisis, Shelby C. Carlson, John A. Vucetich, L Mark Elbroch, Shelby Perry, Lydia A. Roe, Tom Butler, Jeremy T. Bruskotter Dec 2023

The Role Of Governance In Rewilding The United States To Stem The Biodiversity Crisis, Shelby C. Carlson, John A. Vucetich, L Mark Elbroch, Shelby Perry, Lydia A. Roe, Tom Butler, Jeremy T. Bruskotter

Michigan Tech Publications, Part 2

A critical but underattended feature of the biodiversity crisis is the contraction of geographic range experienced by most studied terrestrial vertebrates. In the United States, the primary policy tool for mitigating the biodiversity crisis is a federal law, the Endangered Species Act (ESA). For the past two decades, the federal agencies that administer the ESA have interpreted the act in a manner that precludes treating this geographic element of the crisis. Therefore, the burden of mitigating the biodiversity crisis largely falls on wildlife agencies within state government, which are obligated to operate on behalf of the interests of their constituents. …


Regulation Of Regeneration In Arabidopsis Thaliana, Md Khairul Islam, Sai Teja Mummadi, Sanzhen Liu, Hairong Wei Nov 2023

Regulation Of Regeneration In Arabidopsis Thaliana, Md Khairul Islam, Sai Teja Mummadi, Sanzhen Liu, Hairong Wei

Michigan Tech Publications, Part 2

We employed several algorithms with high efficacy to analyze the public transcriptomic data, aiming to identify key transcription factors (TFs) that regulate regeneration in Arabidopsis thaliana. Initially, we utilized CollaborativeNet, also known as TF-Cluster, to construct a collaborative network of all TFs, which was subsequently decomposed into many subnetworks using the Triple-Link and Compound Spring Embedder (CoSE) algorithms. Functional analysis of these subnetworks led to the identification of nine subnetworks closely associated with regeneration. We further applied principal component analysis and gene ontology (GO) enrichment analysis to reduce the subnetworks from nine to three, namely subnetworks 1, 12, and 17. …


Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John A. Vucetich, Jennifer A. Leonard, Robert K. Wayne Nov 2023

Demographic History Shapes North American Gray Wolf Genomic Diversity And Informs Species' Conservation, Bridgett M. Vonholdt, Daniel R. Stahler, Kristin Brzeski, Marco Musiani, Rolf O. Peterson, Michael Phillips, John Stephenson, Kent Laudon, Erin Meredith, John A. Vucetich, Jennifer A. Leonard, Robert K. Wayne

Michigan Tech Publications, Part 2

Effective population size estimates are critical information needed for evolutionary predictions and conservation decisions. This is particularly true for species with social factors that restrict access to breeding or experience repeated fluctuations in population size across generations. We investigated the genomic estimates of effective population size along with diversity, subdivision, and inbreeding from 162,109 minimally filtered and 81,595 statistically neutral and unlinked SNPs genotyped in 437 grey wolf samples from North America collected between 1986 and 2021. We found genetic structure across North America, represented by three distinct demographic histories of western, central, and eastern regions of the continent. Further, …


Micro-Fibrillated Cellulose In Lignin–Phenol–Formaldehyde Adhesives For Plywood Production, Sheikh Ali Ahmed, Reza Hosseinpourpia, Stergios Adamopoulos Nov 2023

Micro-Fibrillated Cellulose In Lignin–Phenol–Formaldehyde Adhesives For Plywood Production, Sheikh Ali Ahmed, Reza Hosseinpourpia, Stergios Adamopoulos

Michigan Tech Publications, Part 2

Petrochemical-based phenol–formaldehyde (PF) adhesives are widely used in plywood production. To substitute phenol in the synthesis of PF adhesives, lignin can be added due to its structural similarity to phenol. Moreover, micro-fibrillated cellulose (MFC) can further enhance the bond performance, mechanical properties, and toughness of adhesive systems. Thus, the aim of this study was to evaluate the adhesion performance of lignin–PF (LPF) adhesives reinforced with MFC. In LPF formulations, three levels of MFC (0, 15, and 30 wt% based on the total solid content of adhesives) were added to the homogenous adhesive mixture. Three-layer plywood panels from birch (Betula pendula …


Ecological Change And Livestock Governance In A Peruvian National Park, Kenneth R. Young, Eyner Alata, Rodney Chimner, Randall B. Boone, Gillian Bowser, Laura Bourgeau-Chavez, Beatriz Fuentealba, Jessica Gilbert, Javier A. Ñaupari, Molly H. Polk, Sigrid Resh, Cecilia Turin, Melody Zarria-Samanamud Nov 2023

Ecological Change And Livestock Governance In A Peruvian National Park, Kenneth R. Young, Eyner Alata, Rodney Chimner, Randall B. Boone, Gillian Bowser, Laura Bourgeau-Chavez, Beatriz Fuentealba, Jessica Gilbert, Javier A. Ñaupari, Molly H. Polk, Sigrid Resh, Cecilia Turin, Melody Zarria-Samanamud

Michigan Tech Publications, Part 2

While the grazing of livestock has occurred for millennia in the Andes, current sustainability debates center on concerns with co-managing climate change and pastoralism. These discussions have special resonance in places protected by the state for biodiversity, scenery, and sustainable and traditional land uses, such as those found in protected areas and biosphere reserves. For this article, we integrate data from a social-ecological research project on the land use systems that affect high-elevation ecosystems in Peru’s Huascarán National Park, with special emphasis on the wetlands. We used land cover and land use data and insights from interactions with pastoralists to …


A Haplotype-Resolved Chromosome-Scale Genome For Quercus Rubra L. Provides Insights Into The Genetics Of Adaptive Traits For Red Oak Species, Beant Kapoor, Jerry Jenkins, Jeremy Schmutz, Tatyana Zhebentyayeva, Carsten Kulheim, Mark Coggeshall, Chris Heim, Jesse R. Lasky, Laura Leites, Nurul Islam-Faridi, Jeanne Romero-Severson, Victoria L. Deleo, Sarah M. Lucas, Desanka Lazic, Oliver Gailing, John Carlson, Margaret Staton Nov 2023

A Haplotype-Resolved Chromosome-Scale Genome For Quercus Rubra L. Provides Insights Into The Genetics Of Adaptive Traits For Red Oak Species, Beant Kapoor, Jerry Jenkins, Jeremy Schmutz, Tatyana Zhebentyayeva, Carsten Kulheim, Mark Coggeshall, Chris Heim, Jesse R. Lasky, Laura Leites, Nurul Islam-Faridi, Jeanne Romero-Severson, Victoria L. Deleo, Sarah M. Lucas, Desanka Lazic, Oliver Gailing, John Carlson, Margaret Staton

Michigan Tech Publications, Part 2

Northern red oak (Quercus rubra L.) is an ecologically and economically important forest tree native to North America. We present a chromosome-scale genome of Q. rubra generated by the combination of PacBio sequences and chromatin conformation capture (Hi-C) scaffolding. This is the first reference genome from the red oak clade (section Lobatae). The Q. rubra assembly spans 739 Mb with 95.27% of the genome in 12 chromosomes and 33,333 protein-coding genes. Comparisons to the genomes of Quercus lobata and Quercus mongolica revealed high collinearity, with intrachromosomal structural variants present. Orthologous gene family analysis with other tree species revealed that gene …


Coexistence Of Specialist And Generalist Species Within Mixed Plastic Derivative-Utilizing Microbial Communities, Laura G. Schaerer, Lindsay I. Putman, Isaac Bigcraft, Emma Byrne, Daniel G. Kulas, Ali Zolghadr, Sulihat Aloba, Rebecca Ong, David Shonnard, Stephen Techtmann Oct 2023

Coexistence Of Specialist And Generalist Species Within Mixed Plastic Derivative-Utilizing Microbial Communities, Laura G. Schaerer, Lindsay I. Putman, Isaac Bigcraft, Emma Byrne, Daniel G. Kulas, Ali Zolghadr, Sulihat Aloba, Rebecca Ong, David Shonnard, Stephen Techtmann

Michigan Tech Publications, Part 2

BACKGROUND Plastic-degrading microbial isolates offer great potential to degrade, transform, and upcycle plastic waste. Tandem chemical and biological processing of plastic wastes has been shown to substantially increase the rates of plastic degradation however, the focus of this work has been almost entirely on microbial isolates (either bioengineered or naturally occurring). We propose that a microbial community has even greater potential for plastic upcycling. A microbial community has greater metabolic diversity to process mixed plastic waste streams and has built-in functional redundancy for optimal resilience. RESULTS Here, we used two plastic-derivative degrading communities as a model system to investigate the …


Quantification Of Amu River Riverbank Erosion In Balkh Province Of Afghanistan During 2004–2020, Abdul Basir Mahmoodzada, Divyesh Varade, Sawahiko Shimada, Hiromu Okazawa, Shafiqullah Aryan, Gulbuddin Gulab, Abd El Zaher M.A. Mustafa, Humaira Rizwana, Yogesh Ahlawat, Hosam O. Elansary Oct 2023

Quantification Of Amu River Riverbank Erosion In Balkh Province Of Afghanistan During 2004–2020, Abdul Basir Mahmoodzada, Divyesh Varade, Sawahiko Shimada, Hiromu Okazawa, Shafiqullah Aryan, Gulbuddin Gulab, Abd El Zaher M.A. Mustafa, Humaira Rizwana, Yogesh Ahlawat, Hosam O. Elansary

Michigan Tech Publications, Part 2

In this study, we propose quantifying the Amu River riverbank erosion with the modelled river discharge in Kaldar District, Balkh Province of Afghanistan from 2004 to 2020. We propose a framework synergizing multi-source information for modelling the erosion area based on three components: (1) river discharge, (2) river width, and (3) erosion area. The total river discharge for the watershed shared by Afghanistan and Tajikistan was modelled using hydrological parameters from the European Centre for Medium-Range Weather Forecasts (ECMWF) Reanalysis v5 (ERA5) data through multivariate linear regression with ground station data. The river width was determined manually using the Normalized …


A High-Efficiency Gene Silencing In Plants Using Two-Hit Asymmetrical Artificial Micrornas, Sachin Teotia, Xiaoran Wang, Na Zhou, Mengmeng Wang, Haiping Liu, Jun Qin, Dianwei Han, Chingwen Li, Christine E. Li, Shangjin Pan, Haifeng Tang, Wenjun Kang, Zhanhui Zhang, Xiaoqing Tang, Ting Peng, Guiliang Tang Sep 2023

A High-Efficiency Gene Silencing In Plants Using Two-Hit Asymmetrical Artificial Micrornas, Sachin Teotia, Xiaoran Wang, Na Zhou, Mengmeng Wang, Haiping Liu, Jun Qin, Dianwei Han, Chingwen Li, Christine E. Li, Shangjin Pan, Haifeng Tang, Wenjun Kang, Zhanhui Zhang, Xiaoqing Tang, Ting Peng, Guiliang Tang

Michigan Tech Publications

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a crucial role in gene regulation. They are produced through an enzyme-guided process called dicing and have an asymmetrical structure with two nucleotide overhangs at the 3' ends. Artificial microRNAs (amiRNAs or amiRs) are designed to mimic the structure of miRNAs and can be used to silence specific genes of interest. Traditionally, amiRNAs are designed based on an endogenous miRNA precursor with certain mismatches at specific positions to increase their efficiency. In this study, the authors modified the highly expressed miR168a in Arabidopsis thaliana by replacing the single miR168 stem-loop / …


Integrating Forest Structural Diversity Measurement Into Ecological Research, Jeff W. Atkins, Parth Bhatt, Luis Carrasco, Emily Francis, James E. Garabedian, Christopher R. Hakkenberg, Brady S. Hardiman, Jinha Jung, Anil Koirala, Elizabeth A. Larue, Sungchan Oh, Gang Shao, Guofan Shao, H. H. Shugart, Anna Spiers, Atticus E.L. Stovall, Thilina D. Surasinghe, Xiaonan Tai, Lu Zhai, Tao Zhang, Keith Krause Sep 2023

Integrating Forest Structural Diversity Measurement Into Ecological Research, Jeff W. Atkins, Parth Bhatt, Luis Carrasco, Emily Francis, James E. Garabedian, Christopher R. Hakkenberg, Brady S. Hardiman, Jinha Jung, Anil Koirala, Elizabeth A. Larue, Sungchan Oh, Gang Shao, Guofan Shao, H. H. Shugart, Anna Spiers, Atticus E.L. Stovall, Thilina D. Surasinghe, Xiaonan Tai, Lu Zhai, Tao Zhang, Keith Krause

Michigan Tech Publications, Part 2

The measurement of forest structure has evolved steadily due to advances in technology, methodology, and theory. Such advances have greatly increased our capacity to describe key forest structural elements and resulted in a range of measurement approaches from traditional analog tools such as measurement tapes to highly derived and computationally intensive methods such as advanced remote sensing tools (e.g., lidar, radar). This assortment of measurement approaches results in structural metrics unique to each method, with the caveat that metrics may be biased or constrained by the measurement approach taken. While forest structural diversity (FSD) metrics foster novel research opportunities, understanding …


Evaluating Impacts Of R3 Workshops For First-Time Hunters At Universities Across The United States, Richard Von Furstenberg, Victoria R. Vayer, Lincoln R. Larson, M. Nils Peterson, Kangjae Jerry Lee, Kathryn Stevenson, Adam A. Ahlers, Christine Anhalt-Depies, Taniya Bethke, Brian Clark, Kiley M. Davan, Ashley A. Dayer, Todd E. Fisher, Benjamin Ghasemi, Larry Gigliotti, Kris Irwin, Samuel J. Keith, Matt Kelly, Gerard Kyle, Elizabeth Metcalf, Mark D. Needham, Neelam C. Poudyal, Michael Quartuch, Shari L. Rodriguez, Chelsie Romulo, Ryan L. Sharp, William Siemer, Matthew T. Springer, Richard Stedman, Taylor Stein, Timothy R. Van Deelen Sep 2023

Evaluating Impacts Of R3 Workshops For First-Time Hunters At Universities Across The United States, Richard Von Furstenberg, Victoria R. Vayer, Lincoln R. Larson, M. Nils Peterson, Kangjae Jerry Lee, Kathryn Stevenson, Adam A. Ahlers, Christine Anhalt-Depies, Taniya Bethke, Brian Clark, Kiley M. Davan, Ashley A. Dayer, Todd E. Fisher, Benjamin Ghasemi, Larry Gigliotti, Kris Irwin, Samuel J. Keith, Matt Kelly, Gerard Kyle, Elizabeth Metcalf, Mark D. Needham, Neelam C. Poudyal, Michael Quartuch, Shari L. Rodriguez, Chelsie Romulo, Ryan L. Sharp, William Siemer, Matthew T. Springer, Richard Stedman, Taylor Stein, Timothy R. Van Deelen

Michigan Tech Publications, Part 2

Declines in hunter numbers across the United States make hunter recruitment, retention, and reactivation (R3) a high priority for wildlife management. As wildlife management agencies and nongovernmental organizations seek to reach new audiences, college campuses present a unique opportunity to cultivate nontraditional path hunters. Despite recent proliferation of R3 initiatives, little research has evaluated effects of hunting programs on knowledge, attitudes, and behaviors of new hunters. We designed and implemented Getting Started Outdoors: Hunting 101 workshops specifically targeting college students without previous hunting experience, and we assessed workshop efficacy with a theoretically-grounded approach to workshop evaluation. Using quantitative and qualitative …


Object-Detection From Multi-View Remote Sensing Images: A Case Study Of Fruit And Flower Detection And Counting On A Central Florida Strawberry Farm, Caiwang Zheng, Tao Liu, Amr Abd-Elrahman, Vance M. Whitaker, Benjamin Wilkinson Sep 2023

Object-Detection From Multi-View Remote Sensing Images: A Case Study Of Fruit And Flower Detection And Counting On A Central Florida Strawberry Farm, Caiwang Zheng, Tao Liu, Amr Abd-Elrahman, Vance M. Whitaker, Benjamin Wilkinson

Michigan Tech Publications, Part 2

Object detection in remote sensing images is one of the most critical computer vision tasks for various earth observation applications. Previous studies applied object detection models to orthomosaic images generated from the SfM (Structure-from-Motion) analysis to perform object detection and counting. However, some small objects that are occluded from the vertical view but observable in raw images from the oblique views cannot be detected in the orthomosaic image, leading to an occlusion issue that cannot be resolved with the traditional orthophoto-based approach. Taking strawberry detection as a case study, the objective of this study is to detect small objects directly …


Finding The Variables That React: Relationships Between Ability Beliefs And Student Achievement In An Inquiry-Based Introductory Chemistry Laboratory Course, Lindsay B. Wheeler, Amanda L. Gonczi Sep 2023

Finding The Variables That React: Relationships Between Ability Beliefs And Student Achievement In An Inquiry-Based Introductory Chemistry Laboratory Course, Lindsay B. Wheeler, Amanda L. Gonczi

Michigan Tech Publications, Part 2

This quantitative, exploratory study examined whether a Project-Based Guided Inquiry (PBGI) chemistry laboratory course supported more incremental ability beliefs among students, examined achievement outcomes for students grouped based on changes in student-reported chemistry ability beliefs from pre- to post-course, and provided explanations for differences in achievement and chemistry ability beliefs. Data sources included pre/post surveys of 367 undergraduate students' and 18 teaching assistants' (TAs) content knowledge, ability beliefs, sociodemographics (e.g., gender, race), and prior chemistry experience. The hierarchical linear regression (HLR) model accounted for 27.4% of the variance in post-content scores, with male students and students with more prior chemistry …


Reconstructing 42 Years (1979–2020) Of Great Lakes Surface Temperature Through A Deep Learning Approach, Miraj Kayastha, Tao Liu, Daniel Titze, Timothy C. Havens, Chenfu Huang, Pengfei Xue Aug 2023

Reconstructing 42 Years (1979–2020) Of Great Lakes Surface Temperature Through A Deep Learning Approach, Miraj Kayastha, Tao Liu, Daniel Titze, Timothy C. Havens, Chenfu Huang, Pengfei Xue

Michigan Tech Publications, Part 2

Accurate estimates for the lake surface temperature (LST) of the Great Lakes are critical to understanding the regional climate. Dedicated lake models of various complexity have been used to simulate LST but they suffer from noticeable biases and can be computationally expensive. Additionally, the available historical LST datasets are limited by either short temporal coverage (<30 >years) or lower spatial resolution (0.25° × 0.25°). Therefore, in this study, we employed a deep learning model based on Long Short-Term Memory (LSTM) neural networks to produce a daily LST dataset for the Great Lakes that spans an unparalleled 42 years (1979–2020) at …


The Far-Reaching Effects Of Genetic Process In A Keystone Predator Species, Grey Wolves, Sarah Hoy, Philip W. Hedrick, Rolf O. Peterson, Leah Vucetich, Kristin Brzeski, John A. Vucetich Aug 2023

The Far-Reaching Effects Of Genetic Process In A Keystone Predator Species, Grey Wolves, Sarah Hoy, Philip W. Hedrick, Rolf O. Peterson, Leah Vucetich, Kristin Brzeski, John A. Vucetich

Michigan Tech Publications, Part 2

Although detrimental genetic processes are known to adversely affect the viability of populations, little is known about how detrimental genetic processes in a keystone species can affect the functioning of ecosystems. Here, we assessed how changes in the genetic characteristics of a keystone predator, grey wolves, affected the ecosystem of Isle Royale National Park over two decades. Changes in the genetic characteristic of the wolf population associated with a genetic rescue event, followed by high levels of inbreeding, led to a rise and then fall in predation rates on moose, the primary prey of wolves and dominant mammalian herbivore in …


Blood Flow Restriction As A Potential Therapy To Restore Physical Function Following Covid-19 Infection, Isaac J. Wedig, John J. Durocher, John Mcdaniel, Steve Elmer Jul 2023

Blood Flow Restriction As A Potential Therapy To Restore Physical Function Following Covid-19 Infection, Isaac J. Wedig, John J. Durocher, John Mcdaniel, Steve Elmer

Michigan Tech Publications, Part 2

Accumulating evidence indicates that some COVID-19 survivors display reduced muscle mass, muscle strength, and aerobic capacity, which contribute to impairments in physical function that can persist for months after the acute phase of illness. Accordingly, strategies to restore muscle mass, muscle strength, and aerobic capacity following infection are critical to mitigate the long-term consequences of COVID-19. Blood flow restriction (BFR), which involves the application of mechanical compression to the limbs, presents a promising therapy that could be utilized throughout different phases of COVID-19 illness. Specifically, we hypothesize that: 1) use of passive BFR modalities can mitigate losses of muscle mass …


Coastal Environments: Mine Discharges And Infringements On Indigenous Peoples’ Rights, Charles Kerfoot, Gary Swain, Luis M. Verissimo, Erin Johnston, Carol Maclennan, Daniel Schneider, Noel Urban Jul 2023

Coastal Environments: Mine Discharges And Infringements On Indigenous Peoples’ Rights, Charles Kerfoot, Gary Swain, Luis M. Verissimo, Erin Johnston, Carol Maclennan, Daniel Schneider, Noel Urban

Michigan Tech Publications, Part 2

Over a century ago, copper mills on the Keweenaw Peninsula of Lake Superior sluiced 64 million metric tonnes (MMT) of tailings into coastal waters, creating a metal-rich “halo”. Here we show that relatively small discharges can spread widely in time and space. Mass Mill (2.9 MMT) dumping into Lake Superior also illustrates the complexity of interactions with Indigenous Peoples. A combination of aerial photos, LiDAR, and a microscope technique for distinguishing end-member particles traces the migration of tailings. The clay fraction spread rapidly across Keweenaw Bay and curled into terminal L’Anse Bay, within tribal Reservation boundaries. The coarse stamp sand …


Putrescine Mitigates High Temperature Effects By Modulating Morpho-Physiological And Biochemical Attributes In Brassica Juncea Seedlings, Parul Sharma, Nita Lakra, Yogesh Ahlawat, Abbu Zaid, Ahmed M. Abd-Elgawad, Hosam O. Elansary, Anita Gupta Jul 2023

Putrescine Mitigates High Temperature Effects By Modulating Morpho-Physiological And Biochemical Attributes In Brassica Juncea Seedlings, Parul Sharma, Nita Lakra, Yogesh Ahlawat, Abbu Zaid, Ahmed M. Abd-Elgawad, Hosam O. Elansary, Anita Gupta

Michigan Tech Publications, Part 2

A variety of environmental issues are affecting crops all across the world, but rising temperatures are posing the greatest threat. High temperature has been found to drastically inhibit seedling emergence and cause leaf necrosis at the seedling stage, which results in poor plant stand and significantly decreased yields. Polyamines (PAs) are positively charged, low-molecular-weight aliphatic nitrogenous bases present in all living organisms and are involved in various biological processes in plant growth and development, including senescence and response to different abiotic stresses. Putrescine (Put) functions as a master growth regulator that promotes optimal plant development and greater stress tolerance. Here, …


Burned Area And Carbon Emissions Across Northwestern Boreal North America From 2001-2019, Stefano Potter, Sol Cooperdock, Sander Veraverbeke, Xanthe Walker, Michelle C. Mack, Scott J. Goetz, Jennifer Baltzer, Laura Bourgeau-Chavez, Arden Burrell, Catherine Dieleman, Nancy French, Stijn Hantson, Elizabeth E. Hoy, Liza Jenkins, Jill F. Johnstone, Evan S. Kane, Susan M. Natali, James T. Randerson, Merritt R. Turetsky, Ellen Whitman, Elizabeth Wiggins, Brendan M. Rogers Jul 2023

Burned Area And Carbon Emissions Across Northwestern Boreal North America From 2001-2019, Stefano Potter, Sol Cooperdock, Sander Veraverbeke, Xanthe Walker, Michelle C. Mack, Scott J. Goetz, Jennifer Baltzer, Laura Bourgeau-Chavez, Arden Burrell, Catherine Dieleman, Nancy French, Stijn Hantson, Elizabeth E. Hoy, Liza Jenkins, Jill F. Johnstone, Evan S. Kane, Susan M. Natali, James T. Randerson, Merritt R. Turetsky, Ellen Whitman, Elizabeth Wiggins, Brendan M. Rogers

Michigan Tech Publications, Part 2

Fire is the dominant disturbance agent in Alaskan and Canadian boreal ecosystems and releases large amounts of carbon into the atmosphere. Burned area and carbon emissions have been increasing with climate change, which have the potential to alter the carbon balance and shift the region from a historic sink to a source. It is therefore critically important to track the spatiotemporal changes in burned area and fire carbon emissions over time. Here we developed a new burned-area detection algorithm between 2001-2019 across Alaska and Canada at 500 m (meters) resolution that utilizes finer-scale 30 m Landsat imagery to account for …


Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang Jun 2023

Impacts Of Microrna-483 On Human Diseases, Katy Matson, Aaron B. Macleod, Nirali Mehta, Ellie Sempek, Xiaoqing Tang

Michigan Tech Publications

MicroRNAs (miRNAs) are short non-coding RNA molecules that regulate gene expression by targeting specific messenger RNAs (mRNAs) in distinct cell types. This review provides a com-prehensive overview of the current understanding regarding the involvement of miR-483-5p and miR-483-3p in various physiological and pathological processes. Downregulation of miR-483-5p has been linked to numerous diseases, including type 2 diabetes, fatty liver disease, diabetic nephropathy, and neurological injury. Accumulating evidence indicates that miR-483-5p plays a crucial protective role in preserving cell function and viability by targeting specific transcripts. Notably, elevated levels of miR-483-5p in the bloodstream strongly correlate with metabolic risk factors and …


Using Culturally Significant Birds To Guide The Timing Of Prescribed Fires In The Klamath Siskiyou Bioregion, Linda L. Long, Frank L. Lake, Jaime L. Stephens, John D. Alexander, C. John Ralph, Jared D. Wolfe Jun 2023

Using Culturally Significant Birds To Guide The Timing Of Prescribed Fires In The Klamath Siskiyou Bioregion, Linda L. Long, Frank L. Lake, Jaime L. Stephens, John D. Alexander, C. John Ralph, Jared D. Wolfe

Michigan Tech Publications

Historically, wildfire and tribal burning practices played important roles in shaping ecosystems throughout the Klamath Siskiyou Bioregion of northern California and southern Oregon. Over the past several decades, there has been increased interest in the application of fire for forest management through the implementation of prescribed fires within habitats that are used by a diversity of migrant and resident land birds. While many bird species may benefit from habitat enhancements associated with wildfires, cultural burning, and prescribed fire, individuals may face direct or indirect harm. In this study, we analyzed the timing of breeding and molting in 11 species of …


Agroforestry Buffers On Nitrogen Reduction In Groundwater On A Grazed Hillslope, Miguel Salceda, Ranjith P. Udawatta, Stephen H. Anderson, Sidath S. Mendis, Fengjing Liu Jun 2023

Agroforestry Buffers On Nitrogen Reduction In Groundwater On A Grazed Hillslope, Miguel Salceda, Ranjith P. Udawatta, Stephen H. Anderson, Sidath S. Mendis, Fengjing Liu

Michigan Tech Publications

Agricultural practices often contribute to the transport of solutes into groundwater; thus, low-cost strategies that extract nutrients from groundwater are essential to address water pollution. This study evaluated the effects of agroforestry (tree + grass; AB [cottonwood {Populus deltoides Bortr. ex Marsh.}]) and grass buffers (GB; [tall fescue Schedonorus phoenix (Scop.) Holub, Red clover {Trifolium pretense L.}, and Lespedeza {Lespedeza Michx}]) on groundwater nitrogen (N) concentrations. The experiment consisted of two grazing watersheds, one with an AB and another with a GB treatment. Buffers were not grazed since 2001. Three wells representing summit, backslope, and foot-slope positions were installed at …