Life Sciences Commons^™

Pot1 Proteins In Green Algae And Land Plants: Dna-Binding Properties And Evidence Of Co-Evolution With Telomeric Dna, Eugene V. Shakirov, Xiangyu Song, Jessica A. Joseph, Dorothy E. Shippen

Biological Sciences Faculty Research

Telomeric DNA terminates with a single-stranded 3′ G-overhang that in vertebrates and fission yeast is bound by POT1 (Protection Of Telomeres). However, no in vitro telomeric DNA binding is associated with Arabidopsis POT1 paralogs. To further investigate POT1–DNA interaction in plants, we cloned POT1 genes from 11 plant species representing major branches of plant kingdom. Telomeric DNA binding was associated with POT1 proteins from the green alga Ostreococcus lucimarinus and two flowering plants, maize and Asparagus. Site-directed mutagenesis revealed that several residues critical for telomeric DNA recognition in vertebrates are functionally conserved in plant POT1 proteins. However, the plant proteins …

Expression And Function Of Hypoxia Inducible Factor-1 Alpha In Human Melanoma Under Non-Hypoxic Conditions, Caroline N. Mills, Sandeep S. Joshi, Richard M. Niles

Biochemistry and Microbiology

Background

Hypoxia inducible factor-1 alpha (HIF-1α) protein is rapidly degraded under normoxic conditions. When oxygen tensions fall HIF-1α protein stabilizes and transactivates genes involved in adaptation to hypoxic conditions. We have examined the normoxic expression of HIF-1α RNA and protein in normal human melanocytes and a series of human melanoma cell lines isolated from radial growth phase (RGP), vertical growth phase (VGP) and metastatic (MET) melanomas.

Results

HIF-1α mRNA and protein was increased in RGP vs melanocytes, VGP vs RGP and MET vs VGP melanoma cell lines. We also detected expression of a HIF-1α mRNA splice variant that lacks part …

The Importance Of Fallback Foods In Primate Ecology And Evolution, Paul J. Constantino, Barth W. Wright

Biological Sciences Faculty Research

The role of fallback foods in shaping primate ranging, socioecology, and morphology has recently become a topic of particular interest to biological anthropologists. Although the use of fallback resources has been noted in the ecological and primatological literature for a number of decades, few attempts have been made to define fallback foods or to explore the utility of this concept for primate evolutionary biologists and ecologists. As a preface to this special issue of the American Journal of Physical Anthropology devoted to the topic of fallback foods in primate ecology and evolution, we discuss the development and use of the …

Pot1‐Independent Single‐Strand Telomeric Dna Binding Activities In Brassicaceae, Eugene V. Shakirov, Thomas D. Mcknight, Dorothy E. Shippen

Biological Sciences Faculty Research

Telomeres define the ends of linear eukaryotic chromosomes and are required for genome maintenance and continued cell proliferation. The extreme ends of telomeres terminate in a single‐strand protrusion, termed the G‐overhang, which, in vertebrates and fission yeast, is bound by evolutionarily conserved members of the POT1 (protection of telomeres) protein family. Unlike most other model organisms, the flowering plant Arabidopsis thaliana encodes two divergent POT1‐like proteins. Here we show that the single‐strand telomeric DNA binding activity present in A. thaliana nuclear extracts is not dependent on POT1a or POT1b proteins. Furthermore, in contrast to POT1 proteins from yeast and vertebrates, …

Characterization And Differentiation Of Peripheral Blood Derived Multipotent Adult Progenitor Cells, Hari Satya Shankar Addagarla

Theses, Dissertations and Capstones

Stem cells are populations of undifferentiated cells that are found in most tissues and act as precursors for regeneration and maintenance. In the future, they could provide promising therapies for diseases which are to date incurable. Our lab developed a novel cell line from the peripheral blood of adult transgenic green fluorescent protein swine and designated them as Peripheral Blood Derived Multipotent Adult Progenitor Cells (PBD-MAPCs). In this study we characterized the mRNA and protein expression profiles of PBD-MAPCs before and after neural differentiation and investigated the potential of PBD-MAPCs to differentiate into myocardial or neural lineages in vitro. We …

The Occurrence Of Batrachochytrium Dendrobatidis In Salamander Populations Of West Virginia, Casey John Bartkus

Theses, Dissertations and Capstones

Batrachochytrium dendrobatidis (Bd), otherwise known as the amphibian chytrid fungus, is a pathogen that causes the amphibian disease, chytridiomycosis. While Bd has been found in amphibian populations in other states across the country, little is known of its occurrence in West Virginia. Batrachochytrium dendrobatidis is highly associated with amphibians in montane habitats. Because West Virginia has the highest mean elevation of any state east of the Mississippi River, amphibians in the state may be at high risk of an outbreak. The goal of this study was to determine the status of Bd in salamander species of concern in West Virginia. …

Determining The Distribution Of Antibiotic Resistant And Fecal Indicator Bacteria In The Ohio River, Caitlin Nicole Swecker

Theses, Dissertations and Capstones

The Ohio River extends 981 miles from Pittsburgh, PA to Cairo, Ill providing drinking water to over three million people, a natural habitat for aquatic life, a public recreation resource, a major transportation route, and a source of water for industry. The Guyandotte River is a highly impacted tributary emptying into the Ohio River in Huntington, WV. The objectives of this study were to determine if sediment load is correlated to the number of antibiotic resistant bacteria (ARB) and determine if a single surface sample is a sufficient representative measurement of ARB populations in a large river. In 2007, subsurface …

The Natural History Of Cave-Associated Populations Of Eurycea L. Longicauda With Notes On Sympatric Amphibian Species, Kevin Wayne Saunders

Theses, Dissertations and Capstones

The purpose of this study was to collect data on the natural history of the Long-tailed Salamander (Eurycea l. longicauda) in eastern Kentucky and West Virginia. The objectives of this research included characterization of epigean and hypogean habitat for this species, recording distances moved by individuals in populations associated with caves, and collection of data on courtship, oviposition, and larval development. The primary study site was Carter Caves State Park in Olive Hill, Kentucky. This area was visited from October 2007 to December 2008. Salamanders were photographed for identification based on unique pattern aberrancies and distances were measured between the …

Developing A Monitoring Protocol For The Monkey River Watershed, Belize, Central America, Sean Elliott Collins

Theses, Dissertations and Capstones

The study of tropical aquatic systems has been limited. Research in developing countries can be challenging due to inadequate resources and cultural variety. Generally, efforts are concentrated on developing and maintaining economic stability rather than ecological sustainability. The aim of this project was to preliminarily develop and utilize a rapid bioassessment protocol (RBP) for the Monkey River watershed in Belize by determining which metrics best described overall stream health. Like biomonitoring protocols already established for temperate systems, a regional tropical aquatic watershed monitoring program should provide information including stream and watershed health. These protocols score systems on a variety of …

Loss Of Ski Expression In Testicular Cancer Leads To An Enhanced Invasive Phenotype Through Both Bmp-Dependent And Bmp-Independent Pathways, Amy N. Nash

Theses, Dissertations and Capstones

The proto-oncogene SKI is a transcription factor and a co-repressor of the TGFβ superfamily, including TGF

β and BMP. However, additional data suggests that SKI may function as a tumor suppressor in some cell types. The TGFβ superfamily has been implicated in cancer progression and germ cell migration. Testicular cancer afflicts men during their peak reproductive years and is the most common cancer among men of this age group. Cisplatin-based chemotherapy is the standard treatment for testicular cancer. This treatment can lead to undesirable side effects, including infertility. We have shown that SKI expression is decreased in testicular germ cell …

Enumeration Of Endothelial Progenitor Cells In Hind Limb Suspended Rats And The Mechanics Of Endothelial Wound Healing, Jarrod Matthew Pennington

Theses, Dissertations and Capstones

The endothelium repairs itself through two methods. One is by the activity of circulating endothelial progenitor cells (EPCs). EPCs are immature endothelial cells that circulate the bloodstream that are capable of proliferation and differentiation into mature endothelium. It is thought that EPCs contribute to the repair and replacement of damaged endothelial cells in the process of reendothelialization. Physical inactivity and bed rest are known to be deleterious to the endothelium. It is possible that this inactivity is also deleterious to the number of viable EPCs, which would reduce the ability of the endothelium to repair itself. We used hind limb …

High Resolution Single Molecule Optical Localization Of Multiple Fluorophores On Dna Origami Constructs Fluorophores On Dna Origami Constructs, Anuradha Rajulapati

Theses, Dissertations and Capstones

An ongoing challenge in the development of nanoelectronics and nanophotonics is the nondestructive, high-resolution localization in space of single molecules and multi-molecular assemblies. The apparent barrier to the use of optical microscopy at the sub-100 nm scale is the well known Abbe Limit, the diffraction limit to resolution. This laboratory has adapted a technique called Single-molecule high resolution imaging with photobleaching (SHRIMP). We have developed methods for utilizing SHRIMP for the determination of the separation of two fluorophores in single DNA origami constructs.

DNA Origami is extremely useful because it can address nanocomponents down to 2nm separation. It should be …

Prevalence Of Intestinal Parasite Infection In Symptomatic And Asymptomatic Dogs In Southwestern West Virginia: The Potential Impact On Human Health, Tashina Marie Savilla

Theses, Dissertations and Capstones

Most dog owners are unaware of asymptomatic infection and of the possibility of contracting zoonotic parasites from their dogs. We hypothesized that parasite infection is: associated with one or more symptoms; independent of gender and age; and independent of anthelmintic usage. Stool samples were collected from 231 dogs in Kanawha County, West Virginia, and were examined by simple fecal flotation. Parasitic prevalence was found to be 23% for Ancylostoma caninum, 8% for Trichuris vulpis, 7% for Toxocara canis, 4% for Isospora species, and 32.5% overall. There was no significant relationship between infection and gender, nor between infection and symptom for …

Demographics, Activity, And Habitat Selection Of The Eastern Box Turtle (Terrapene C. Carolina) In West Virginia, Justin Adam Weiss

Theses, Dissertations and Capstones

Little is known about the eastern box turtle, Terrapene c. carolina, in West Virginia. The purpose of this study was to compare demographic data between two populations of box turtles, to determine which environmental parameter(s) influence activity patterns, and to describe the optimal habitat of the box turtles at two study sites (Lake and State Park) at Beech Fork Wildlife Management Area. I observed male-skewed sex ratios and low numbers of juveniles in both populations. Male turtles were larger than females in carapace length, but females were larger in carapace height. Turtles at the Lake site exhibited greater body mass …

Distribution And Status Of The Northern Leopard Frog, Rana Pipiens, In West Virginia, Amanda Nicole Spriggs

Theses, Dissertations and Capstones

The Northern Leopard Frog, Rana pipiens, is listed as a species of concern by the West Virginia Division of Natural Resources. Because this species is uncommon in West Virginia, information is needed to understand its distribution and conservation needs. I surveyed 14 historic R. pipiens locations and 70 Wildlife Management Areas throughout West Virginia beginning in March 2008. Rana pipiens was found in four locations; Greenbottom Wildlife Management Area was confirmed through both auditory and visual surveys and the rest were confirmed through visual observations. Besides Greenbottom Wildlife Management Area, all other sites were confirmed by the presence of a …

A Study Of The Morphology Of Tatenectes Laramiensis, A Cryptocleidoid Plesiosaur From The Sundance Formation (Wyoming, Usa), Hallie P. Street

Theses, Dissertations and Capstones

Tatenectes laramiensis is a cryptocleidoid plesiosaur from the Late Jurassic Sundance Formation. This study primarily concerns a partial skeleton comprised of dorsal vertebrae, ribs, gastralia, and pelvic girdle. Even though the skeleton is incomplete, it is hypothesized that the preserved fossils are sufficient to indicate the overall body shape and also the preferred habitat of Tatenectes. Possibly the most notable of the novel characters is the pachyostotic state of the gastralia. In relation to the ribs and overall size of Tatenectes, in comparison to the gastralia in related taxa, these gastralia are disproportionately robust. The combination of these morphologies suggests …

Application Of Remote Sensing To Examine Changes In Bird Communities Over A Gradient Of Urbanization, John E. Northeimer

Theses, Dissertations and Capstones

Interest in urban ecology and the impact of urbanization on natural resources has increased over the last decade. Studies have related degradation of water quality and loss of biodiversity to increased urbanization. Avian species comprise an important part of the urban ecology. Surveys of bird populations can provide insight into the effects of urbanization on species composition, diversity, and distribution. A search of available literature revealed no published comprehensive avian surveys completed during the breeding season within the city of Huntington, West Virginia. The purpose of this study was to provide an initial characterization of land bird populations during the …

A Reconnaissance Of The Intersex Condition In Micropterus Dolomieu Of The Upper Ohio River Basin As An Indicator Of Anthropogenic Endocrine Disruptors, Christopher Barry

Theses, Dissertations and Capstones

The intersex condition has been used as a marker of endocrine disruption in West Virginia. Fifty-two male smallmouth bass from 6 basins on the upper Ohio River were examined for the presence and severity of intersex. Vitellogenin (VTG) RNA levels were quantified as marker of estrogenic contaminants. A significant increase in intersex (p=0.02), ranging from 83.3% to 100%, corresponds with higher population densities and agricultural land (p=0.003). At reference sites, smallmouth bass had intersex frequencies of 16.7% to 33.3%. Vitellogenin results were significantly lower (p< 0.001) at impacted sites, consistent with previously reported results in female fish. VTG levels in males were not significantly different. Increased intersex occurrence in males and lowered VTG expression in females have shown that agricultural chemicals and common residential wastewater constituents are likely targets for exploring the relationship between individual EDCs and intersex. There may be a natural background level of the intersex condition.

Distribution Of Woodland Salamanders Of The Valley And Ridge In West Virginia, H. Reid Downer

Theses, Dissertations and Capstones

Two terrestrial woodland salamanders of the genus Plethodon, P. punctatus and P. virginia, are endemic to the Valley and Ridge Physiographic Province in West Virginia and Virginia and occupy limited geographic ranges. Two common and widespread species, P. cylindraceus and P. cinereus, also inhabit this region. To evaluate the distribution and habitat of these terrestrial salamanders I surveyed wooded ridges and slopes throughout the geographic range of the two endemic species by conducting daytime cover object searches and nocturnal visual encounter surveys. From March through November 2008, I recorded the presence of 321 woodland salamanders at 91 sites. I used …

Effects Of Aging On Regulators Of Muscle Apoptosis In The Female F344bn Rat, Murali K. Gadde

Theses, Dissertations and Capstones

Age-related muscle atrophy is a consequence of normal aging characterized by decreases in muscle mass and strength. The mechanism(s) underlying the loss of muscle mass with increasing age is not fully understood, however recent data has suggested that muscle cell apoptosis may be involved. Here we investigate how aging affects the regulation of muscle apoptosis in the extensor digitorum longus (EDL) and soleus muscles of young (6-month), aged (26-month), and very aged (30-month) female Fischer 344/NNiaHSD X Brown Norway / BiNia (F344BN) rats. EDL and soleus muscle mass/body weight ratios were lower in aged animals but not different between 26- …

The Influence Of Fallback Foods On Great Ape Tooth Enamel, Paul J. Constantino, Peter W. Lucas, James J.-W. Lee, Brian R. Lawn

Biological Sciences Faculty Research

Lucas and colleagues recently proposed a model based on fracture and deformation concepts to describe how mammalian tooth enamel may be adapted to the mechanical demands of diet (Lucas et al.: Bioessays 30[2008] 374-385). Here we review the applicability of that model by examining existing data on the food mechanical properties and enamel morphology of great apes (Pan, Pongo, and Gorilla). Particular attention is paid to whether the consumption of fallback foods is likely to play a key role in influencing great ape enamel morphology. Our results suggest that this is indeed the case. We also consider the implications of …

A Plesiosaur Containing An Ichthyosaur Embryo As Stomach Contents From The Sundance Formation Of The Bighorn Basin, Wyoming, F. Robin O’Keefe, Hallie P. Street, Jean Pierre Cavigelli, John J. Socha, R. Dennis O'Keefe

Biological Sciences Faculty Research

Herein we report the discovery of an ichthyosaur embryo from the Upper Member of the Sundance Formation (Oxfordian) of the Bighorn Basin, Wyoming. The specimen is the first known ichthyosaur embryo from the Upper Jurassic, and is the first Jurassic ichthyosaur embryo from North America. The embryo was discovered in close association with the abdomen of an articulated partial plesiosaur skeleton, and several lines of evidence support the interpretation of the embryo as plesiosaur stomach contents. The small size and extremely poor ossification of the embryo indicate that the animal was probably not a neonate. Although the taxonomic affinities of …

Osteology Of The Cryptocleidoid Plesiosaur Tatenectes Laramiensis, With Comments On The Taxonomic Status Of The Cimoliasauridae, F. Robin O’Keefe, Hallie P. Street

Biological Sciences Faculty Research

Recent field work in the Bighorn Basin, Wyoming has recovered significant new material of the plesiosaur Tatenectes laramiensis. The majority of cryptocleidoid plesiosaurs have been recovered from Middle and Upper Jurassic units (Oxford and Kimmeridge Clays, respectively) in the United Kingdom, but Tatenectes laramiensis is one of at least two cryptocleidoids known from the Upper Sundance Member of the Sundance Formation (Oxfordian) of North America. Although poorly known, they bear directly on both the phylogeny and biogeography of the cryptocleidoid plesiosaurs. Here we describe new fossil material of Tatenectes, and reevaluate the phylogenetic position of this genus based on all …

Beheading First: On Nabokov's Translation Of Lewis Carroll, Victor Fet

Biological Sciences Faculty Research

Anya v Strane chudes, young Nabokov’s 1923 Russian translation of Alice’s Adventures in Wonderland, contains an intentionally shifted statement “beheading first, sentence later” compared to Lewis Carroll’s “sentence first, verdict later”. The shift is fitting for the 1920s children émigré audience.