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Pot1‐Independent Single‐Strand Telomeric Dna Binding Activities In Brassicaceae, Eugene V. Shakirov, Thomas D. Mcknight, Dorothy E. Shippen
Pot1‐Independent Single‐Strand Telomeric Dna Binding Activities In Brassicaceae, Eugene V. Shakirov, Thomas D. Mcknight, Dorothy E. Shippen
Biological Sciences Faculty Research
Telomeres define the ends of linear eukaryotic chromosomes and are required for genome maintenance and continued cell proliferation. The extreme ends of telomeres terminate in a single‐strand protrusion, termed the G‐overhang, which, in vertebrates and fission yeast, is bound by evolutionarily conserved members of the POT1 (protection of telomeres) protein family. Unlike most other model organisms, the flowering plant Arabidopsis thaliana encodes two divergent POT1‐like proteins. Here we show that the single‐strand telomeric DNA binding activity present in A. thaliana nuclear extracts is not dependent on POT1a or POT1b proteins. Furthermore, in contrast to POT1 proteins from yeast and vertebrates, …