Life Sciences Commons^™

Animal Pain And The Social Role Of Science, Leslie Irvine

Leslie Irvine, PhD

Assuming that all animals are sentient would mean ending their use in most scientific research. This does not necessarily imply an unscientific or anti-scientific stance. Examining the social role of science reveals its considerable investment in preserving the status quo, including the continued use of animal subjects. From this perspective, the use of animal subjects is a custom that science could move beyond, rather than a methodological requirement that it must defend.

Wildlife Conservation, Zoos And Animal Protection: A Strategic Analysis, Andrew N. Rowan

Andrew N. Rowan, DPhil

The publication consists of the proceedings of a workshop, sponsored by the Gilman Foundation, and held in April of 1994 at the White Oak Conservation Center in Florida. About thirty participants were invited from zoos, animal protection groups and academic institutions to discuss concepts such as wild, captive and tame; animal well-being in the wild and in zoos; and protecting individuals versus conserving populations. In order to maximize the time engaged in discussion, several individuals were identified to prepare target articles which were distributed to all participants before the meeting. These articles form the main chapters in this book. Other …

The State Of The Animals: 2001, Deborah J. Salem, Andrew N. Rowan

Andrew N. Rowan, DPhil

How has the state of animals improved in the last half century? How has it worsened? Where are gains made on behalf of animals under threat? In one landmark volume, distinguished scholars and experts examine these questions–and offer often-provocative answers–for farm animals, companion animals, laboratory animals, zoo animals, and wildlife worldwide.

The State Of The Animals Iv: 2007, Deborah J. Salem, Andrew N. Rowan

Andrew N. Rowan, DPhil

In the fourth volume of the State of the Animals series, a stellar array of researchers, scholars, and leaders in the field explores current and emerging issues in animal protection.

The State Of The Animals Iii: 2005, Deborah J. Salem, Andrew N. Rowan

Andrew N. Rowan, DPhil

In this third, all new, volume in the State of the Animals series, scholars and experts in animal protection examine the challenges facing companion animals, marine mammals, and nonhuman primates and review legal protection for animals here and abroad.

The Animal Research Controversy: Protest, Process & Public Policy, Andrew N. Rowan, Franklin M. Loew, Joan C. Weer

Andrew N. Rowan, DPhil

The controversy today regarding the use of animals in research appears on the surface to be a strongly polarized struggle between the scientific community and the animal protection movement. However, there is a wide range of opinions and philosophies on both sides. Mistrust between the factions has blossomed while communication has withered. Through the 1960s, 1970s and early 1980s, the animal movement grew in numbers and financial resources, and developed much greater public recognition and political clout. The research community paid relatively little attention to the animal movement for much of this period but, alarmed by several public relations coups …

Outdoor Cats: An Animal Welfare And Protection Perspective, John Hadidian, Inga Gibson, Susan Hagood, Nancy Peterson, Bernard Unti, Betsy Mcfarland, Katie Lisnik, Heather Bialy, Inga Fricke, Kathleen Schatzmann, Jennifer Fearing, Pam Runquist, Andrew N. Rowan (Ed.)

Andrew N. Rowan, DPhil

First raised as a serious conservation issue more than 100 years ago, the impact of free-roaming cats on wildlife has been a subject of debate, controversy, and conflict since then. Cats have been tied directly to the extinction of sensitive species in island environments and implicated as major threats to certain wildlife populations elsewhere. Yet the study of free-roaming cats and the problems attributed to them lags behind the standards of research typical with more traditional vertebrate “pest” species. Alternative management approaches, ranging from traditional practices such as removal and depopulation to emerging concepts such as Trap- Neuter-Return (TNR), have …

Outdoor Cats: An Animal Welfare And Protection Perspective, John Hadidian, Inga Gibson, Susan Hagood, Nancy Peterson, Bernard Unti, Betsy Mcfarland, Katie Lisnik, Heather Bialy, Inga Fricke, Kathleen Schatzmann, Jennifer Fearing, Pam Runquist, Andrew N. Rowan (Ed.)

Bernard Unti, PhD

First raised as a serious conservation issue more than 100 years ago, the impact of free-roaming cats on wildlife has been a subject of debate, controversy, and conflict since then. Cats have been tied directly to the extinction of sensitive species in island environments and implicated as major threats to certain wildlife populations elsewhere. Yet the study of free-roaming cats and the problems attributed to them lags behind the standards of research typical with more traditional vertebrate “pest” species. Alternative management approaches, ranging from traditional practices such as removal and depopulation to emerging concepts such as Trap- Neuter-Return (TNR), have …

A Standardized G‐Banded Karyotype For The Raccoon (Procyon Lotor) Compared With The Domestic Cat, Roscoe Stanyon, Francesca Bigoni, Johannes Weinberg, John Hadidian

John Hadidian, PhD

We propose a standardized karyotype for the raccoon (Procyon lotor; 2n = 38, FN 74) and compare it with that of the domestic cat (2n = 38, FN 72). Numerous chromosomes (12) have similar and sometimes identical G-banding and 14 chromosome pairs have remained intact. Other chromosomes apparently differ by Robertsonian translocations and inversions. The conservation of these karyotypes is remarkable considering that the divergence of procyonids and felids predates 50 million years B.P. However, the common diploid number of 38 is not a primitive retention, as sometimes hypothesized. Instead, cats and raccoons converged on this chromosome number by a …

Dc Birdscape: A Program For Monitoring Neotropical Migratory Birds In Washington, Dc, John Sauer, John Hadidian, Sam Droege, Paul Handly, Carolyn Williams, Christopher Swarth, George Didden, Jane Huff

John Hadidian, PhD

Urban and suburban habitats often contain a variety of Neotropical migratory birds, but are poorly sampled by programs such as the North American Breeding Bird Survey. DC Birdscape was developed to inventory and monitor birds in Washington, DC. Birds were surveyed using a systematic sample of point counts during 1993-1995. Results indicate that species richness of Neotropical migratory birds varied among land-use categories, and that maximum species richness occurred in parkland habitats. Although DC Birdscape has provided relevant information on bird distribution and species richness, it is unclear whether the information is of sufficient management interest to support its continuation …

The Relationship Of Animal Protection Interests To Animal Damage Management: Historic Paths, Contemporary Concerns And The Uncertain Future, John Hadidian

John Hadidian, PhD

More than a decade ago Schmidt (1989) called for consideration of animal welfare to become a "firstorder" decision rule in wildlife management concerns, including animal damage control. Although there has been movement in that direction, this clearly has not yet come to pass. This paper takes a brief look at the interests we call animal damage management, animal welfare and protection, animal rights, and environmentalism in order to speculate about their shared concerns and the uncertain future before them. Since animal damage and the management of that damage cannot be abstracted from the environmental context in which they occur, this …

What Is A Humane Wildlife Control Service?, John Griffin, Lori Thiele, Pamela Lough, Janet Snyder, Maggie Brasted, John Hadidian

John Hadidian, PhD

In May 2007, The Humane Society of the United States launched a for-fee business called Humane Wildlife Servicessm to engage in wildlife control jobs in the Washington, D.C. metro area. We had several purposes in launching this service. First, we felt it necessary to offer a service to customers in our home base area that allowed them to choose a wildlife removal company that did not trap and relocate, or trap and kill, animals. Second, we wished to directly experience and test the operational and conceptual challenges associated with this sort of service. Third, we wished to develop a model …

“Nuisance” Wildlife Control Trapping: Another Perspective, Brad Gates, John Hadidian, Laura Simon

John Hadidian, PhD

Urban wildlife control is a rapidly growing profession in which many practitioners apparently still come from a recreational or commercial trapping background. Perhaps for that reason, much of the “control” in resolving human-wildlife conflicts in cities and suburbs seems to revolve around the use of lethal traps to eliminate “problem” animals. Although some states allow relocation and most apparently allow for nuisance animals to be released on site, the extent to which these practices occur is little known. Further, the biological impacts of continual trapping cycles on urban wildlife populations remain little known as well. An alternative approach to trapping …

The “Nuisance” Wildlife Control Industry: Animal Welfare Concerns, John Hadidian, Laura J. Simon, Michele R. Childs

John Hadidian, PhD

The recent and rapid growth of the private “nuisance” wildlife control industry follows the unparalleled current period of urban and suburban expansion. Nuisance wildlife control businesses range from simple home-based services to sophisticated franchised businesses. The nuisance wildlife control operator may hold an advanced degree in the wildlife sciences, or simply be an entrepreneur without formal education or even background experience in wildlife. State and federal agencies may participate directly or indirectly in nuisance wildlife control, in activities ranging from dissemination of advice or information to actual participation in programs that may lead to removal of animals. Naturally, all of …

Salinity Gradient Power (Sgp): A Developmental Roadmap Covering Existing Generation Technologies And Recent Investigative Results Into The Feasibility Of Bipolar Membrane-Based Salinity Gradient Power Generation, Clifford R. Merz, Wilfrido A. Moreno, Marilyn Barger, Stephen M. Lipka

Clifford Merz

Besides wind and solar-based renewable energy technologies, marine sources are being actively discussed. Sources of marine renewable energy traditionally have included ocean currents, ocean waves, tides, thermal gradients, and salinity gradients. Salinity gradient power (SGP) is an attractive marine renewable resource because it possesses not only the largest energy potential but likely the largest total available resource as well. SGP is instantly available when diluted and concentrated ionic solutions are mixed; is renewable, sustainable, and produces no CO₂ emissions or other significant effluents that may interfere with global climate. The ultimate challenge is in the economics of the recovery …

Effect Of Acetylation On The Mechanical And Thermal Properties Of Soy Flour Elastomers, Kendra A. Allen, Sarah Cady, David Grewell

Sarah Cady

Biobased fillers were utilized as components in soy-elastomer composites. Soy flour is lightweight, low cost, and high strength, which makes it an ideal alternative to petroleum-derived fillers. However, poor interfacial adhesion and low dispersion within the polymer matrix are limiting factors for composite performance. Soy flour chemically pretreated by acetylation was compounded with synthetic rubber elastomers. In general, soy flour (as received) concentration in the composite is proportional to the ultimate strength. However, soy-elastomer composites with acetylated filler performed similar to the neat elastomer. In addition, the pretreated composite's thermal stability increased and exhibited less phase seperation compared to the …

“Everyday” Knowledge And A New Paradigm Of Animal Research, David Fraser, Jeffrey M. Spooner, Catherine A. Schuppli

David Fraser, PhD

Commentary on Marino and Allen (2017) The Psychology of Cows

International Consensus Principles For Ethical Wildlife Control, Sara Dubois, Nicole Fenwick, Erin A. Ryan, Liv Baker, Sandra E. Baker, Ngaio J. Beausoleil, Scott Carter, Barbara Cartwright, Federico Costa, Chris Draper, John Griffin, Adam Grogan, Gregg Howald, Bidda Jones, Kate E. Littin, Amanda T. Lombard, David J. Mellor, Daniel Ramp, Catherine A. Schuppli, David Fraser

David Fraser, PhD

Human–wildlife conflicts are commonly addressed by excluding, relocating, or lethally controlling animals with the goal of preserving public health and safety, protecting property, or conserving other valued wildlife. However, declining wildlife populations, a lack of efficacy of control methods in achieving desired outcomes, and changes in how people value animals have triggered widespread acknowledgment of the need for ethical and evidence-based approaches to managing such conflicts. We explored international perspectives on and experiences with human–wildlife conflicts to develop principles for ethical wildlife control. A diverse panel of 20 experts convened at a 2-day workshop and developed the principles through a …

Distributions Of Hypothalamic Neuron Populations Co-Expressing Tyrosine Hydroxylase And The Vesicular Gaba Transporter In The Mouse., Kenichiro Negishi, Mikayla A. Payant, Kayla S. Schumacker, Gabor Wittman, Rebecca M. Butler, Ronald M. Lechan, Harry W. M. Steinbusch M, Arshad M. Khan, Melissa J. Chee

Arshad M. Khan, Ph.D.

No abstract provided.

Act On The Registration And Evaluation Of Chemicals (K-Reach) And Replacement, Reduction Or Refinement Best Practices, Soojin Ha, Troy Seidle, Kyung-Min Lim

Troy Seidle, PhD

Objectives - Korea’s Act on the Registration and Evaluation of Chemicals (K-REACH) was enacted for the protection of human health and the environment in 2015. Considering that about 2000 new substances are introduced annually across the globe, the extent of animal testing requirement could be overwhelming unless regulators and companies work proactively to institute and enforce global best practices to replace, reduce or refine animal use. In this review, the way to reduce the animal use for K-REACH is discussed. Methods - Background of the enforcement of the K-REACH and its details was reviewed along with the papers and regulatory …

Mental Stress From Animal Experiments: A Survey With Korean Researchers, Minji Kang, Ahram Han, Da-Eun Kim, Troy Seidle, Kyung-Min Lim, Seungjin Bae

Troy Seidle, PhD

Animal experiments have been widely conducted in the life sciences for more than a century, and have long been a subject of ethical and societal controversy due to the deliberate infliction of harm upon sentient animals. However, the harmful use of animals may also negatively impact the mental health of researchers themselves. We sought to evaluate the anxiety level of researchers engaged in animal use to analyse the mental stress from animal testing. The State Anxiety Scale of the State-Trait Anxiety Inventory (STAI) was used to evaluate how researchers feel when they conduct animal, as opposed to non-animal, based experiments …

The Efficacy Of Whole Human Genome Capture On Ancient Dental Calculus And Dentin, Kirsten A. Ziesemer, Jazmin Ramos-Madrigal, Allison E. Mann, Bernd W. Brandt, Krithivasan Sankaranarayanan, Andrew T. Ozga, Menno Hoogland, Courtney A. Hofman, Domingo C. Salazar-Garcia, Bruno Frohlich, George R. Miller, Anne C. Stone, Mark Aldenderfer, Cecil M. Lewis Jr., Corinne L. Hofman, Christina Warinner, Hannes Schroeder

Andrew Ozga

Objectives

Dental calculus is among the richest known sources of ancient DNA in the archaeological record. Although most DNA within calculus is microbial, it has been shown to contain sufficient human DNA for the targeted retrieval of whole mitochondrial genomes. Here, we explore whether calculus is also a viable substrate for whole human genome recovery using targeted enrichment techniques.

Materials and methods

Total DNA extracted from 24 paired archaeological human dentin and calculus samples was subjected to whole human genome enrichment using in‐solution hybridization capture and high‐throughput sequencing.

Results

Total DNA from calculus exceeded that of dentin in all cases, …

Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner

Andrew Ozga

Objectives

Archaeological dental calculus is a rich source of host‐associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis.

Materials and …

Subsistence Strategies In Traditional Societies Distinguish Gut Microbiomes, Alexandra J. Obregon-Tito, Raul Y. Tito, Jessica Metcalf, Krithivasan Sankaranarayanan, Jose C. Clemente, Luke K. Ursell, Zhenjiang Zech Xu, Will Van Treuren, Rob Knight, Patrick M. Gaffney, Paul Spicer, Paul Lawson, Luis Marin-Reyes, Omar Trujillo-Villarroel, Morris Foster, Emilio Guija-Poma, Luzmila Troncoso-Corzo, Christina Warinner, Andrew T. Ozga, Cecil M. Lewis Jr.

Andrew Ozga

Recent studies suggest that gut microbiomes of urban-industrialized societies are different from those of traditional peoples. Here we examine the relationship between lifeways and gut microbiota through taxonomic and functional potential characterization of faecal samples from hunter-gatherer and traditional agriculturalist communities in Peru and an urban-industrialized community from the US. We find that in addition to taxonomic and metabolic differences between urban and traditional lifestyles, hunter-gatherers form a distinct sub-group among traditional peoples. As observed in previous studies, we find that Treponema are characteristic of traditional gut microbiomes. Moreover, through genome reconstruction (2.2–2.5 MB, coverage depth × 26–513) and functional …

Intrinsic Challenges In Ancient Microbiome Reconstruction Using 16s Rrna Gene Amplification, Kirsten Ziesemer, Allison Mann, Krithivasan Sankaranarayanan, Hannes Schroeder, Andrew T. Ozga, Bernd W. Brandt, Egija Zaura, Andrea Waters-Rist, Menno Hoogland, Domingo C. Salazar-Garcia, Mark Aldenderfer, Camilla Speller, Jessica Hendy, Darlene A. Weston, Sandy J. Macdonald, Gavin H. Thomas, Matthew J. Collins, Cecil M. Lewis Jr., Corinne Hofman, Christina Warinner

Andrew Ozga

To date, characterization of ancient oral (dental calculus) and gut (coprolite) microbiota has been primarily accomplished through a metataxonomic approach involving targeted amplification of one or more variable regions in the 16S rRNA gene. Specifically, the V3 region (E. coli341–534) of this gene has been suggested as an excellent candidate for ancient DNA amplification and microbial community reconstruction. However, in practice this metataxonomic approach often produces highly skewed taxonomic frequency data. In this study, we use non-targeted (shotgun metagenomics) sequencing methods to better understand skewed microbial profiles observed in four ancient dental calculus specimens previously analyzed by amplicon …

Origins Of An Unmarked Georgia Cemetery Using Ancient Dna Analysis, Andrew T. Ozga, Raul Y. Tito, Brian M. Kemp, Hugh Matternes, Alexandra J. Obregon-Tito, Leslie Neal, Cecil M. Lewis Jr.

Andrew Ozga

Determining the origins of those buried within undocumented cemeteries is of incredible importance to historical archaeologists and, in many cases, the nearby communities. In the case of Avondale Burial Place, a cemetery in Bibb County, Georgia, in use from 1820 to 1950, all written documentation of those interred within it has been lost. Osteological and archaeological evidence alone could not describe, with confidence, the ancestral origins of the 101 individuals buried there. In the present study, we used ancient DNA extraction methods in well-preserved skeletal fragments from 20 individuals buried in Avondale Burial Place to investigate the origins of the …

The Farmers' Millennium: The Ideology Of Agricultural Improvement In Iowa, 1855 To 1865, Michael Belding Iii

Michael Belding III

The Morrill Act of 1862, a piece of federal legislation enacted a century and a half ago, lives on today. That law allocated thousands of acres of federal land to state governments, based on the size of their congressional delegations, so they could establish colleges of agriculture and the mechanic arts and give a college education, liberal and practical, to students who could not otherwise afford one. The Morrill Act lives on because the "land-grant colleges" it endowed with financial resources still exist today, operating on billion-dollar budgets and enrolling tens of thousands of students. Further, at least at Iowa …

Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone

Andrew Ozga

Objectives

The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi‐tropical sites in Tanzania, Mexico, and Puerto Rico (N = 12).

Materials and Methods

All samples were extracted twice, built into double‐stranded sequencing libraries, and shotgun sequenced on the Illumina HiSeq 2500. The first extraction protocol, Method D, was previously designed for …

Differential Preservation Of Endogenous Human And Microbial Dna In Dental Calculus And Dentin, Allison E. Mann, Susanna Sabin, Kirsten Ziesemer, Ashild J. Vagene, Hannes Schroeder, Andrew T. Ozga, Krithivasan Sankaranarayanan, Courtney A. Hofman, James A. Fellows Yates, Domingo C. Salazar-Garcia, Bruno Frohlich, Mark Aldenderfer, Menno Hoogland, Christopher Read, George R. Milner, Anne C. Stone, Cecil M. Lewis Jr., Johannes Krause, Corinne Hofman, Kirsten I. Bos, Christina Warinner

Andrew Ozga

Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is …

Marine Invertebrates: Communities At Risk, Jennifer A. Mather

Jennifer Mather, PhD

Our definition of the word ‘animal’ centers on vertebrates, yet 99% of the animals on the planet are invertebrates, about which we know little. In addition, although the Census of Marine Life (COML.org) has recently conducted an extensive audit of marine ecosystems, we still do not understand much about the animals of the seas. Surveys of the best-known ecosystems, in which invertebrate populations often play a key role, show that the invertebrate populations are affected by human impact. Coral animals are the foundation of coral reef systems, which are estimated to contain 30% of the species in the ocean. Physical …