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- Scanning Electron Microscopy (15)
- Scanning electron microscopy (15)
- X-ray microanalysis (7)
- Freeze-drying (5)
- Low temperature embedding (5)
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- Transmission electron microscopy (5)
- Collagen (4)
- Cryoultramicrotomy (4)
- Freeze-substitution (4)
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- Culture (2)
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- Electron energy loss spectroscopy (2)
Articles 121 - 122 of 122
Full-Text Articles in Life Sciences
Immunocytochemical Labeling Of Enzymes In Low Temperature Embedded Plant Tissue: The Precursor Of Glyoxysomal Malate Dehydrogenase Is Located In The Cytosol Of Watermelon Cotyledon Cells, C. Sautter
Scanning Electron Microscopy
The Lowicryl-technique in combination with protein A gold was used in order to localize the precursor of glyoxysomal malate dehydrogenase in watermelon cotyledons. Preservation of the antigen was evaluated by a preembedding technique in isolated organelles. The glyoxysomal malate dehydrogenase was localized in tissue sections by a postembedding technique. Antigens of glyoxysomal malate dehydrogenase were found in the glyoxysomal matrix and in the cytosol, whereas the endoplasmic reticulum was completely free of labeling. Controls are presented by preimmunserum, by a serum against various proteins of the glyoxysomal membrane and by application of cycloheximide in order to inhibit translation at cytosolic …
To Resin Or Not To Resin In Immunocytochemistry, J. J. Wolosewick
To Resin Or Not To Resin In Immunocytochemistry, J. J. Wolosewick
Scanning Electron Microscopy
Hydrated resinless sections produced by a variety of methods (cryoultramicrotomy or polyethylene glycol techniques) appear to be excellent specimens for post-embedding immunocytochemistry at the electron microscopic level. A perplexing problem is the lack of apparent penetration throuqhout the section thickness of particulate probes such as ferritin or colloidal gold. This report draws attention to the possible causes for this phenomenon namely the size of the probe and the possible effects of fixation or processing. Smaller probes, gentler fixation or permeabilization procedures and increased incubation times all seem to be logical approaches to increasing penetration of immunoreagents into thick hydrated sections.