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Articles 61 - 90 of 2070
Full-Text Articles in Life Sciences
Characterizing The Performance And Behaviors Of Runners Using Twitter, Qian He, Emmanuel Agu, Diane Strong, Bengisu Tulu, Peder Pedersen
Characterizing The Performance And Behaviors Of Runners Using Twitter, Qian He, Emmanuel Agu, Diane Strong, Bengisu Tulu, Peder Pedersen
Emmanuel O. Agu
Running is a popular physical activity that improves physical and mental wellbeing. Unfortunately, up-to- date information about runners’ performance and psychological wellbeing is limited. Many questions remain unanswered, such as how far and how fast runners typically run, their preferred running times and frequencies, how long new runners persist before dropping out, and what factors cause runners to quit. Without hard data, establishing patterns of runner behavior and mitigating the challenges they face are difficult. Collecting data manually from large numbers of runners for research studies is costly and time consuming. Emerging Social Networking Services (SNS) and fitness tracking devices …
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko
Fgf2-Induced Effects On Transcriptome Associated With Regeneration Competence In Adult Human Fibroblasts, Olga Kashpur, David Lapointe, Sakthikumar Ambady, Elizabeth Ryder, Tanja Dominko
Sakthikumar Ambady
BACKGROUND: Adult human fibroblasts grown in low oxygen and with FGF2 supplementation have the capacity to tip the healing outcome of skeletal muscle injury - by favoring regeneration response in vivo over scar formation. Here, we compare the transcriptomes of control adult human dermal fibroblasts and induced regeneration-competent (iRC) fibroblasts to identify transcriptional changes that may be related to their regeneration competence. RESULTS: We identified a unique gene-expression profile that characterizes FGF2-induced iRC fibroblast phenotype. Significantly differentially expressed genes due to FGF2 treatment were identified and analyzed to determine overrepresented Gene Ontology terms. Genes belonging to extracellular matrix components, adhesion …
Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, Matthew Sazinsky, Benjamin Lemoine, Maria Orofino, Roman Davydov, Krisztina Bencze, Timothy Stemmler, Brian Hoffman, José Argüello, Amy Rosenzweig
Characterization And Structure Of A Zn2+ And [2fe-2s]-Containing Copper Chaperone From Archaeoglobus Fulgidus, Matthew Sazinsky, Benjamin Lemoine, Maria Orofino, Roman Davydov, Krisztina Bencze, Timothy Stemmler, Brian Hoffman, José Argüello, Amy Rosenzweig
José M. Argüello
Bacterial CopZ proteins deliver copper to P1B-type Cu+-ATPases that are homologous to the human Wilson and Menkes disease proteins. The genome of the hyperthermophile Archaeoglobus fulgidus encodes a putative CopZ copper chaperone that contains an unusual cysteine rich N-terminal domain of 130 amino acids in addition to a C-terminal copper-binding domain with a conserved CXXC motif. The N-terminal domain (CopZ-NT) is homologous to proteins found only in extremophiles and is the only such protein that is fused to a copper chaperone. Surprisingly, optical, electron paramagnetic resonance, and X-ray absorption spectroscopic data indicate the presence of a [2Fe-2S] cluster in CopZ-NT. …
Rural Juvenile Delinquency: Problems And Needs In East Tennessee, William Bolton, David Brown
Rural Juvenile Delinquency: Problems And Needs In East Tennessee, William Bolton, David Brown
David C. Brown
No abstract provided.
Fresh From The Farm... And Into The Classroom, Margaret Haase, Andrea Azuma, Robert Gottlieb, Mark Vallianatos
Fresh From The Farm... And Into The Classroom, Margaret Haase, Andrea Azuma, Robert Gottlieb, Mark Vallianatos
Mark Vallianatos
No abstract provided.
Systematically Controlling For The Influence Of Age, Sex, Hertz And Time Post-Whole-Body Vibration Exposure On Four Measures Of Physical Performance In Community-Dwelling Older Adults: A Randomized Cross-Over Study, Harold Merriman, C. Brahler, Kurt Jackson
Systematically Controlling For The Influence Of Age, Sex, Hertz And Time Post-Whole-Body Vibration Exposure On Four Measures Of Physical Performance In Community-Dwelling Older Adults: A Randomized Cross-Over Study, Harold Merriman, C. Brahler, Kurt Jackson
C. Jayne Brahler
Though popular, there is little agreement on what whole-body vibration (WBV) parameters will optimize performance. This study aimed to clarify the effects of age, sex, hertz, and time on four physical function indicators in community-dwelling older adults (N = 32). Participants were exposed to 2 minutes WBV per session at either 2 Hz or 26 Hz, and outcome measures were recorded at 2-, 20-, and 40-minute post-WBV. Timed get-up-and-go and chair sit-and-reach performances improved post-WBV for both sexes, were significantly different between 2 Hz and 26 Hz treatments (P ≤ 0.05), and showed statistically significant interactions between age and gender …
Use And Selection Of Sap Trees By Yellow-Bellied Sapsuckers, Laurie Eberhardt
Use And Selection Of Sap Trees By Yellow-Bellied Sapsuckers, Laurie Eberhardt
Laurie Eberhardt
No abstract provided.
An Annotated Bibliography Of References To Historical Distributions Of Pronghorn In Southern And Baja California, David Brown, Jorge Cancino, Kevin Clark, Myrna Smith, Jim Yoakum
An Annotated Bibliography Of References To Historical Distributions Of Pronghorn In Southern And Baja California, David Brown, Jorge Cancino, Kevin Clark, Myrna Smith, Jim Yoakum
David C. Brown
Recent pronghorn (Antilocapra americana) translocations to southern California and the establishment of captive populations of endangered desert pronghorn have revived interest in the historical occurrence of pronghorn in the Californias. Adding to this interest is the recent widespread replacement of coastal sage scrub vegetation in southern California by annual grasslands more favorable to pronghorn. We have searched the scientific and popular literature, as well as museum collections, to locate pronghorn antelope occurrences from below San Francisco Bay southward through the Baja California peninsula. Our results show that pronghorn were widely distributed, and often abundant, on nearly all of the plains …
Human Cryptochrome Exhibits Light-Dependent Magnetosensitivity, Lauren Foley, Robert Gegear, Steven Reppert
Human Cryptochrome Exhibits Light-Dependent Magnetosensitivity, Lauren Foley, Robert Gegear, Steven Reppert
Robert J. Gegear
Humans are not believed to have a magnetic sense, even though many animals use the Earth's magnetic field for orientation and navigation. One model of magnetosensing in animals proposes that geomagnetic fields are perceived by light-sensitive chemical reactions involving the flavoprotein cryptochrome (CRY). Here we show using a transgenic approach that human CRY2, which is heavily expressed in the retina, can function as a magnetosensor in the magnetoreception system of Drosophila and that it does so in a light-dependent manner. The results show that human CRY2 has the molecular capability to function as a light-sensitive magnetosensor and reopen an area …
Invasion By Exotic Earthworms Alters Litter- And Soil-Dwelling Oribatid Mites, Jordan Burke, John Maerz, Joseph Milanovich, Melanie Fisk, Kamal Gandhi
Invasion By Exotic Earthworms Alters Litter- And Soil-Dwelling Oribatid Mites, Jordan Burke, John Maerz, Joseph Milanovich, Melanie Fisk, Kamal Gandhi
Joseph Milanovich
Exotic earthworms are drivers of biotic communities in invaded North American forest stands. Here we used ecologically important oribatid mite (Arachnida: Acari) communities, as model organisms to study the responses of litter- and soil-dwelling microarthropod communities to exotic earthworm invasion in a northern temperate forest. Litter- and soil-dwelling mites were sampled in 2008–2009 from forest areas: (1) with no earthworms; (2) those with epigeic and endogeic species, including Lumbricus rubellus Hoffmeister; and (3) those with epigeic, endogeic, and anecic earthworms including L. terrestris L. Species richness and diversity of litter- and soil-dwelling (0–2 cm soil depth) oribatid mites was 1–2 …
Rural People In Skilled Industrial Work: Experience Of Graduates Of A Manpower Training Program In Appalachia, David Brown, Samuel Cooper
Rural People In Skilled Industrial Work: Experience Of Graduates Of A Manpower Training Program In Appalachia, David Brown, Samuel Cooper
David C. Brown
No abstract provided.
Advancing Synthetic Ecology: Database System To Facilitate Complex Ecological Meta-Analyses, V. Bala Chaudhary
Advancing Synthetic Ecology: Database System To Facilitate Complex Ecological Meta-Analyses, V. Bala Chaudhary
V. Bala Chaudhary
No abstract provided.
Food Access, Availability, And Affordability In 3 Los Angeles Communities, Project Cafe, 2004-2006, Andrea Azuma, Susan Gilliland, Mark Vallianatos, Robert Gottlieb
Food Access, Availability, And Affordability In 3 Los Angeles Communities, Project Cafe, 2004-2006, Andrea Azuma, Susan Gilliland, Mark Vallianatos, Robert Gottlieb
Mark Vallianatos
Introduction Racial/ethnic minority communities are at increasingly high risk for chronic diseases related to obesity. Access to stores that sell affordable, nutritious food is a prerequisite for adopting a healthful diet. The objective of this study was to evaluate food access, availability, and affordability in 3 nonoverlapping but similar low-income communities in urban Los Angeles, California. Methods Using a community-based participatory research approach, we trained community members to conduct a food assessment to 1) map the number and type of retail food outlets in a defined area and 2) survey a sample of stores to determine whether they sold selected …
Effect Of Prior Anterior Superior Iliac Spine Compression Testing On Second Assessor Findings: Implications For Inter-Examiner Reliability Testing, Frank Casella, Michael Kuchera, Jeffrey Nelson, Veronica Ferencz, Nicole Myers
Effect Of Prior Anterior Superior Iliac Spine Compression Testing On Second Assessor Findings: Implications For Inter-Examiner Reliability Testing, Frank Casella, Michael Kuchera, Jeffrey Nelson, Veronica Ferencz, Nicole Myers
Michael Kuchera
BACKGROUND: Osteopathic physicians use palpation to diagnose sacroiliac joint somatic dysfunction (SD) -- including the Anterior Superior Iliac Spine (ASIS) Compression Test for dysfunctional side lateralization. (Literature suggests right-sided lateralization in 80% of asymptomatic individuals). Accurate, reliable tests are crucial however to diagnose SD and kappa (κ) analysis is a gold-standard to determine the degree of interexaminer reliability for tests. Few studies have examined the effect the palpatory examination has on subsequent diagnostic findings and therefore on κ-values.
The Effect Of Yoga Postures On Balance, Flexibility, And Strength In Healthy High School Females, Betsy Donahoe-Fillmore, C. Brahler, Mary Fisher, Kelly Beasley
The Effect Of Yoga Postures On Balance, Flexibility, And Strength In Healthy High School Females, Betsy Donahoe-Fillmore, C. Brahler, Mary Fisher, Kelly Beasley
C. Jayne Brahler
Objective: The purpose of this study was to document the effects of yoga interventions on balance, flexibility, and strength in adolescent girls 14 to 18 years. Study Design: Quasi-experimental, nonrandomized. Background: Research on the effects of yoga on balance, flexibility, and strength has focused on adults, although yoga is being marketed to all ages. Methods and Measures: A convenience sample of 33 female adolescents participated in yoga training 2 times per week and a walking program 3 times per week, for 7 weeks. The instructor-led group received instruction from a registered yoga therapist in person, while the video-led group watched …
Untangling The Biological Contributions To Soil Stability In Semiarid Shrublands, V. Bala Chaudhary, Matthew Bowker, Thomas O'Dell, James Grace, Andrea Redman, Mathias Rillig, Nancy Johnson
Untangling The Biological Contributions To Soil Stability In Semiarid Shrublands, V. Bala Chaudhary, Matthew Bowker, Thomas O'Dell, James Grace, Andrea Redman, Mathias Rillig, Nancy Johnson
V. Bala Chaudhary
Communities of plants, biological soil crusts (BSCs), and arbuscular mycorrhizal (AM) fungi are known to influence soil stability individually, but their relative contributions, interactions, and combined effects are not well understood, particularly in arid and semiarid ecosystems. In a landscape-scale field study we quantified plant, BSC, and AM fungal communities at 216 locations along a gradient of soil stability levels in southern Utah, USA. We used multivariate modeling to examine the relative influences of plants, BSCs, and AM fungi on surface and subsurface stability in a semiarid shrubland landscape. Models were found to be congruent with the data and explained …
Macronuclear Genome Sequence Of The Ciliate Tetrahymena Thermophila, A Model Eukaryote, Jonathan Eisen, Robert Coyne, Martin Wu, Dongying Wu, Mathangi Thiagarajan, Jennifer Wortman, Jonathan Badger, Qinghu Ren, Paolo Amedeo, Kristie Jones, Luke Tallon, Arthur Delcher, Steven Salzberg, Joana Silva, Brian Haas, William Majoros, Maryam Farzad, Jane Carlton, Roger Smith, Jyoti Garg, Ronald Pearlman, Kathleen Karrer, Lei Sun, Gerard Manning, Nels Elde, Aaron Turkewitz, David Asai, David Wilkes, Yufeng Wang, Hong Cai, Kathleen Collins, B. Stewart, Suzanne Lee, Katarzyna Wilamowska, Zasha Weinberg, Walter Ruzzo, Dorota Wloga, Jacek Gaertig, Joseph Frankel, Che-Chia Tsao, Martin Gorovsky, Patrick Keeling, Ross Waller, Nicola Patron, J. Cherry, Nicholas Stover, Cynthia Krieger, Christina Del Toro, Hilary Ryder, Sondra Williamson, Rebecca Barbeau, Eileen Hamilton, Eduardo Orias
Macronuclear Genome Sequence Of The Ciliate Tetrahymena Thermophila, A Model Eukaryote, Jonathan Eisen, Robert Coyne, Martin Wu, Dongying Wu, Mathangi Thiagarajan, Jennifer Wortman, Jonathan Badger, Qinghu Ren, Paolo Amedeo, Kristie Jones, Luke Tallon, Arthur Delcher, Steven Salzberg, Joana Silva, Brian Haas, William Majoros, Maryam Farzad, Jane Carlton, Roger Smith, Jyoti Garg, Ronald Pearlman, Kathleen Karrer, Lei Sun, Gerard Manning, Nels Elde, Aaron Turkewitz, David Asai, David Wilkes, Yufeng Wang, Hong Cai, Kathleen Collins, B. Stewart, Suzanne Lee, Katarzyna Wilamowska, Zasha Weinberg, Walter Ruzzo, Dorota Wloga, Jacek Gaertig, Joseph Frankel, Che-Chia Tsao, Martin Gorovsky, Patrick Keeling, Ross Waller, Nicola Patron, J. Cherry, Nicholas Stover, Cynthia Krieger, Christina Del Toro, Hilary Ryder, Sondra Williamson, Rebecca Barbeau, Eileen Hamilton, Eduardo Orias
Jonathan A. Eisen Ph.D.
The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and …
Mysterious Mycorrhizae? A Field Trip And Classroom Experiment To Demystify The Symbioses Formed Between Plants And Fungi, Nancy Johnson, V. Bala Chaudhary, Jason Hoeksema, John Moore, Anne Pringle
Mysterious Mycorrhizae? A Field Trip And Classroom Experiment To Demystify The Symbioses Formed Between Plants And Fungi, Nancy Johnson, V. Bala Chaudhary, Jason Hoeksema, John Moore, Anne Pringle
V. Bala Chaudhary
No abstract provided.
Cd4+ Regulatory T Cells Require Ctla-4 For The Maintenance Of Systemic Tolerance, Randall Friedline, David Brown, Hai Nguyen, Hardy Kornfeld, Jinhee Lee, Yi Zhang, Mark Appleby, Sandy Der, Joonsoo Kang, Cynthia Chambers
Cd4+ Regulatory T Cells Require Ctla-4 For The Maintenance Of Systemic Tolerance, Randall Friedline, David Brown, Hai Nguyen, Hardy Kornfeld, Jinhee Lee, Yi Zhang, Mark Appleby, Sandy Der, Joonsoo Kang, Cynthia Chambers
David C. Brown
Cytotoxic T lymphocyte antigen-4 (CTLA-4) plays a critical role in negatively regulating T cell responses and has also been implicated in the development and function of natural FOXP3(+) regulatory T cells. CTLA-4-deficient mice develop fatal, early onset lymphoproliferative disease. However, chimeric mice containing both CTLA-4-deficient and -sufficient bone marrow (BM)-derived cells do not develop disease, indicating that CTLA-4 can act in trans to maintain T cell self-tolerance. Using genetically mixed blastocyst and BM chimaeras as well as in vivo T cell transfer systems, we demonstrate that in vivo regulation of Ctla4(-/-) T cells in trans by CTLA-4-sufficient T cells is …
Correlations Between Oxygen Affinity And Sequence Classifications Of Plant Hemoglobins, Benoit Smagghe, Julie Hoy, Ryan Percifield, Suman Kundu, Mark Hargrove, Gautam Sarath, Jean-Louis Hilbert, Richard Watts, Elizabeth Dennis, W. James Peacock, Sylvia Dewilde, Luc Moens, George Blouin, John Olson, Cyril Appleby
Correlations Between Oxygen Affinity And Sequence Classifications Of Plant Hemoglobins, Benoit Smagghe, Julie Hoy, Ryan Percifield, Suman Kundu, Mark Hargrove, Gautam Sarath, Jean-Louis Hilbert, Richard Watts, Elizabeth Dennis, W. James Peacock, Sylvia Dewilde, Luc Moens, George Blouin, John Olson, Cyril Appleby
John Olson
Plants express three phylogenetic classes of hemoglobins (Hb) based on sequence analyses. Class 1 and 2 Hbs are full-length globins with the classical eight helix Mb-like fold, whereas Class 3 plant Hbs resemble the truncated globins found in bacteria. With the exception of the specialized leghemoglobins, the physiological functions of these plant hemoglobins remain unknown. We have reviewed and, in some cases, measured new oxygen binding properties of a large number of Class 1 and 2 plant nonsymbiotic Hbs and leghemoglobins. We found that sequence classification correlates with distinct extents of hexacoordination with the distal histidine and markedly different overall …
Phosphorylation Keeps Pten Phosphatase Closed For Business, Alonzo Ross, Arne Gericke
Phosphorylation Keeps Pten Phosphatase Closed For Business, Alonzo Ross, Arne Gericke
Arne Gericke
No abstract provided.
Organizational Problems Of Small Watersheds, David Brown, Joseph Winsett
Organizational Problems Of Small Watersheds, David Brown, Joseph Winsett
David C. Brown
No abstract provided.
Defining Behavioral And Molecular Differences Between Summer And Migratory Monarch Butterflies, Haisun Zhu, Robert Gegear, Amy Casselman, Sriramana Kanginakudru, Steven Reppert
Defining Behavioral And Molecular Differences Between Summer And Migratory Monarch Butterflies, Haisun Zhu, Robert Gegear, Amy Casselman, Sriramana Kanginakudru, Steven Reppert
Robert J. Gegear
BACKGROUND: In the fall, Eastern North American monarch butterflies (Danaus plexippus) undergo a magnificent long-range migration. In contrast to spring and summer butterflies, fall migrants are juvenile hormone deficient, which leads to reproductive arrest and increased longevity. Migrants also use a time-compensated sun compass to help them navigate in the south/southwesterly direction en route for Mexico. Central issues in this area are defining the relationship between juvenile hormone status and oriented flight, critical features that differentiate summer monarchs from fall migrants, and identifying molecular correlates of behavioral state. RESULTS: Here we show that increasing juvenile hormone activity to induce summer-like …
Do Species Distribution Models Predict Species Richness In Urban And Natural Green Space? A Case Study Using Amphibians, Joseph Milanovich, William Peterman, Kyle Barrett, Matthew Hopton
Do Species Distribution Models Predict Species Richness In Urban And Natural Green Space? A Case Study Using Amphibians, Joseph Milanovich, William Peterman, Kyle Barrett, Matthew Hopton
Joseph Milanovich
No abstract provided.
Effect Of Sugars On Artemisinin Production In Artemisia Annua L.: Transcription And Metabolite Measurements, Patrick Arsenault, Daniel Vail, Kristin Wobbe, Pamela Weathers
Effect Of Sugars On Artemisinin Production In Artemisia Annua L.: Transcription And Metabolite Measurements, Patrick Arsenault, Daniel Vail, Kristin Wobbe, Pamela Weathers
Kristin K. Wobbe
The biosynthesis of the valuable sesquiterpene anti-malarial, artemisinin, is known to respond to exogenous sugar concentrations. Here young Artemisia annua L. seedlings (strain YU) were used to measure the transcripts of six key genes in artemisinin biosynthesis in response to growth on sucrose, glucose, or fructose. The measured genes are: from the cytosolic arm of terpene biosynthesis, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), farnesyl disphosphate (FPS); from the plastid arm of terpene biosynthesis, 1-deoxyxylulose-5-phosphate synthase (DXS), 1-deoxyxylulouse 5-phosphate reductoisomerase (DXR); from the dedicated artemisinin pathway amorpha-4,11-diene synthase (ADS), and the P450, CYP71AV1 (CYP). Changes in intracellular concentrations of artemisinin (AN) and its precursors, …
Diet Of Western Slimy Salamander, Plethodon Albagula (Caudata: Plethodontidae), From Two Mountain Ranges In Arkansas, Joseph Milanovich, Stanley Trauth, Tanja Mckay
Diet Of Western Slimy Salamander, Plethodon Albagula (Caudata: Plethodontidae), From Two Mountain Ranges In Arkansas, Joseph Milanovich, Stanley Trauth, Tanja Mckay
Joseph Milanovich
We identified stomach contents of 80 Plethodon albagula (Western Slimy Salamander) from two mountain ranges in Arkansas (Ozark and Ouachita) to examine if regional differences in diet occur. Museum specimens from 1985 to 2005 were used from locations throughout each mountain range. Although a wide variety of prey were found in stomachs, Hymenoptera: Formicidae and Coleoptera: Carabidae were found to be the most important food items in the diet of P. albagula. Ants and beetles constituted 85% abundance of their total diet (79.6% Ozark and 90.1% Ouachita) and 52.2% of their total biomass (42.6% Ozark and 64.1% Ouachita). Seventy-eight and …
Numerous Test Items In The Complete And Short Forms Of The Bot-2 Do Not Contribute Substantially To Motor Performance Assessments In Typically Developing Children Six To Ten Years Old, C. Brahler, Betsy Donahoe-Fillmore, Sara Mrowzinski, Susan Aebker, Megan Kreill
Numerous Test Items In The Complete And Short Forms Of The Bot-2 Do Not Contribute Substantially To Motor Performance Assessments In Typically Developing Children Six To Ten Years Old, C. Brahler, Betsy Donahoe-Fillmore, Sara Mrowzinski, Susan Aebker, Megan Kreill
C. Jayne Brahler
The purposes of the current study were (1) to determine the magnitude of association between individual subtest items and the respective total subtest scores on the four subtests in the Bruininks-Oseretsky Test of Motor Proficiency, second edition (BOT-2) and (2) to review items on the BOT-2 Short Form. The correlation coefficients (r) between ranged from .865 to .071. Several items suffered from a ceiling effect, and four of the eight items on the Short Form for these four subtests had very low associations with their subtest total score. These results raise several issues with the BOT-2 that warrant further investigation.
Urspelerpes Brucei, Carlos Campbell, Trip Lamb, Joseph Milanovich
Urspelerpes Brucei, Carlos Campbell, Trip Lamb, Joseph Milanovich
Joseph Milanovich
No abstract provided.
Disease Will Limit Future Food Supply From The Global Crustacean Fishery And Aquaculture Sectors, G. Stentiford, D. Neil, E. Peeler, J. Shields, H. Small, T. Flegel, J. Vlak, B. Jones, F. Morado, S. Moss, J. Lotz, Lyric Bartholomay, D. Behringer, C. Hauton, D. Lightner
Disease Will Limit Future Food Supply From The Global Crustacean Fishery And Aquaculture Sectors, G. Stentiford, D. Neil, E. Peeler, J. Shields, H. Small, T. Flegel, J. Vlak, B. Jones, F. Morado, S. Moss, J. Lotz, Lyric Bartholomay, D. Behringer, C. Hauton, D. Lightner
Lyric Bartholomay
Seafood is a highly traded food commodity. Farmed and captured crustaceans contribute a significant proportion with annual production exceeding 10 M metric tonnes with first sale value of $40bn. The sector is dominated by farmed tropical marine shrimp, the fastest growing sector of the global aquaculture industry. It is significant in supporting rural livelihoods and alleviating poverty in producing nations within Asia and Latin America while forming an increasing contribution to aquatic food supply in more developed countries. Nations with marine borders often also support important marine fisheries for crustaceans that are regionally traded as live animals and commodity products. …
Differential Effects Of Egf And Tgf-Beta1 On Fibroblast Activity In Fibrin-Based Tissue Equivalents, Jaime Grouf, Angela Throm, Jenna Balestrini, Katie Bush, Kristen Billiar
Differential Effects Of Egf And Tgf-Beta1 On Fibroblast Activity In Fibrin-Based Tissue Equivalents, Jaime Grouf, Angela Throm, Jenna Balestrini, Katie Bush, Kristen Billiar
Kristen L. Billiar
Transforming growth factor-beta1 (TGF-beta1) is commonly used to promote matrix production for engineered tissues in vitro, yet it also enhances fibroblast contractility. For applications where contraction is undesirable, we hypothesized that epidermal growth factor (EGF) would yield equivalent mechanical properties without enhancing contractility. In this study, the response of human dermal fibroblasts to EGF (5 ng/mL) and TGF-beta1 (5 ng/mL) was determined within hemispheric fibrin-based gels by assessing matrix compaction and strength, cell number, collagen production, and contractility. After 3 weeks, both cytokines enhanced compaction relative to controls, and EGF roughly doubled matrix strength over controls and TGF-beta1-treated samples. TGF-beta1 …