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Development Of An Efficient Plant Regeneration System In Sunflower(Helianthus Annuus L.), Ekrem Gürel, Kemal Kazan
Development Of An Efficient Plant Regeneration System In Sunflower(Helianthus Annuus L.), Ekrem Gürel, Kemal Kazan
Turkish Journal of Botany
With the aim of developing an efficient plant regeneration system from cells or tissues of sunflower ( Helianthus annuus L.), we compared several regeneration protocols using different explant types and hormonal combinations. Somatic embryogenesis could be induced on cotyledon explants, especially from the basal (proximal) portion of the cotyledons, but genotypic vairation appeared to be the most critical factor for both somatic embryo and root production. Such a variation was more prominent when 10 different sunflower cultivars were compared for shoot production from shoot-tip explants. Further refinement of the culture conditions may be neceassary to improve the efficiency of somatic …
Plant Regeneration From Unfertilized Ovaries Of Sugar Beet ( Beta Vulgaris L.)Cultured In Vitro, Ekrem Gürel, Songül Gürel
Plant Regeneration From Unfertilized Ovaries Of Sugar Beet ( Beta Vulgaris L.)Cultured In Vitro, Ekrem Gürel, Songül Gürel
Turkish Journal of Botany
A. method is described for plant regeneration from unfertilized ovaries isolated from a diploid male sterile sugar beet ( Beta vulgaris L.) breeding line that was developed at the Sugar Institute, Ankara, Turkey. Ovary explants were cultured on Murashige & Skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP). Two treatments were tested by incubating all of the explants in darkness for 15 days, and then transferring one half to light and keeping the other half in darkness throughout the culture. Callus formation occurred in both treatments more or less at similar rates: however, there was a significant difference between the …