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Full-Text Articles in Life Sciences
Establishment Of Cell Suspension Cultures And Plant Regeneration In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Ekrem Gürel, Zeki̇ Kaya
Establishment Of Cell Suspension Cultures And Plant Regeneration In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Ekrem Gürel, Zeki̇ Kaya
Turkish Journal of Botany
The establishment of cell suspension culture from the callus of sugar beet (Beta vulgaris L.) breeding lines and plant regeneration from suspension-derived callus are described in this study. Using different concentrations and combinations of BAP and 2,4-D, the growth patterns of cell suspension cultures were examined during a range of culture periods (0, 3, 5, 7, 9, 11, 13 and 15 days). In all lines, the growth rates of cells were initially slow but as the culture proceeded, they increased significantly and accumulated great amounts of biomass over a period of 15 days. Medium containing high BAP (0.25 mg/l) and …
Callus Production And Plant Regeneration From Anther Culture Of Some Turkish Barley Cultivars, Çi̇ğdem Savaşkan, Ivona Szarejko, M. Ci̇hat Toker
Callus Production And Plant Regeneration From Anther Culture Of Some Turkish Barley Cultivars, Çi̇ğdem Savaşkan, Ivona Szarejko, M. Ci̇hat Toker
Turkish Journal of Botany
Anther culture of four different barley cultivars ( Hordeum vulgareL. cvs. Anadolu, Cumhuriyet-50, Obruk-86 and Tokak-157/37) currently cultivated in Turkey has been investigated. Two different culture media (BAC3 and FHG) were used and the effect of a 21-day cold pretreatment was studied. Androgenesis on BAC3 medium was evaluated statistically in all genotypes and the percentage of anther response and the frequency of calli production were found significantly different (P
Plant Regeneration From Unfertilized Ovaries Of Sugar Beet ( Beta Vulgaris L.)Cultured In Vitro, Ekrem Gürel, Songül Gürel
Plant Regeneration From Unfertilized Ovaries Of Sugar Beet ( Beta Vulgaris L.)Cultured In Vitro, Ekrem Gürel, Songül Gürel
Turkish Journal of Botany
A. method is described for plant regeneration from unfertilized ovaries isolated from a diploid male sterile sugar beet ( Beta vulgaris L.) breeding line that was developed at the Sugar Institute, Ankara, Turkey. Ovary explants were cultured on Murashige & Skoog (MS) medium containing 2.0 mg/l benzylaminopurine (BAP). Two treatments were tested by incubating all of the explants in darkness for 15 days, and then transferring one half to light and keeping the other half in darkness throughout the culture. Callus formation occurred in both treatments more or less at similar rates: however, there was a significant difference between the …
Development Of An Efficient Plant Regeneration System In Sunflower(Helianthus Annuus L.), Ekrem Gürel, Kemal Kazan
Development Of An Efficient Plant Regeneration System In Sunflower(Helianthus Annuus L.), Ekrem Gürel, Kemal Kazan
Turkish Journal of Botany
With the aim of developing an efficient plant regeneration system from cells or tissues of sunflower ( Helianthus annuus L.), we compared several regeneration protocols using different explant types and hormonal combinations. Somatic embryogenesis could be induced on cotyledon explants, especially from the basal (proximal) portion of the cotyledons, but genotypic vairation appeared to be the most critical factor for both somatic embryo and root production. Such a variation was more prominent when 10 different sunflower cultivars were compared for shoot production from shoot-tip explants. Further refinement of the culture conditions may be neceassary to improve the efficiency of somatic …