Life Sciences Commons^™

The Life Cycle Of Lissorchis Heterorchis, Macy And Krygier (1969), Brendan P. H. Onyejekwe

Dissertations and Theses

Many Flumenicola virens (Lea) collected from Crystal Springs Creek and Tualatin River, Portland, Oregon, were found to shed large numbers of a species of microcercous cercariae containing refractile granules. They resembled the cercariae of Triganodistomum mutabile (=Lissorchis mutabile Cort, 1918 as described by Wallace (1939), and subsequent experiments proved that they were the larval stages of what was described by Macy and Krygier (1969), as Lissorchis heterorchis. Encystment of these cercariae was induced experimentally in uninfected adult brown planarians collected partly from Crystal Springs Creek and partly from Carolina Biological Research Station in Gladstone, Portland, Oregon. Adult flukes were obtained …

Post-Mortem Examination Of Cattle And Sheep For Parasitic Helminths, Phil Duke Wright

Theses and Dissertations

A survey of helminth parasites of sheep and cattle was conducted to determine the incidence and distribution of these parasites that could be detected by post-mortem examination. Animals were obtained from local abattoirs within the valley, and the viscera were examined for the presence of helminth parasites. Parasites identifies from sheep in order of highest incidence were: Haemonchus contorus, Ostertagia cicumcinta, Chabertia ovina, Nematodirus spathiger, Trichostongylus colubriformis, Thysanosoma actinioides, Trichuris ovis, N. filicollis, Echinococcus granulosus, Strongyloides papillosus, O. ostertagi, Cooperia oncophora, Cysticercus tenuicollis, Fasciola hepatica, Oesophagostomum venulosum, Moniezia expansa, and M. benedieni. Those recovered from cattle in order of highest …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Effect Of Splenectomy On Relapse Of Hemal Parasites In Pigeons, Phillip James Sprino

Masters Theses

No abstract provided.

Protozoan And Helminth Parasites Of White-Tailed Deer (Odocoileus Virginianus) In Southern Illinois, Sarah M. Mugwanya

Masters Theses

No abstract provided.

The Effect Of The Parasite Hymenolepis Diminuta On Iodine Economy In The Rat, David Gourdon Holmes

Theses and Dissertations

A balance of iodine within the rats is important for the maintenance of normal thyroid function. Because of Hymenolepis d[imi]nuta's affinity for certain nutrients, it may upset norma[l] iodine balance in the rat. To determine this, tapeworm-infected and non-infected rats were given 125Iodide orally and the percent uptake of iodide by the thyroid and the amount of radioac[tiv]ity in the tapeworms was determined. The percent distribution of the radio-iodinated compounds in the thyroid, tapeworms and feces was also determined. These procedures were repeated except 125Thyroxine was administered orally. There was a decrease in percent iod[id]e uptake by thyroids of tapeworm-infected …

Infectivity And Development Of Trichinella Spiralis In Germ-Free, Monocontaminated And Conventional Mice, Daulat Sitanggang

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of the experiment was to compare the development of Trichinella spiralis in germ-free, Lactobacillus casei monocontaminated, Escherichia coli monocontaminated and conventional mice. The criteria used in the experiments were the recovery of adults and larvae of T. spiralis after 72 hours and one month post-infection, respectively. which gave an indication of the effects of no bacteria or only a certain, single species of bacterium on the development of this nematode in mice.

Germ-free T. spiralis larvae were obtained by washing them several times with sterile normal saline, and treatment with antibiotics. The gavaging procedure was done without anesthetization …

A Survey Of The Helminth Parasites And Coccidia Of Cattle And Sheep In Utah Valley, Joseph Carl Fox

Theses and Dissertations

A survey of gastro-intestinal parasites in cattle and sheep in Utah Valley was conducted. Cattle in the valley were not seriously parasitized by helminths, whereas sheep usually had heavy helminth infections. Ten genera of helminths and 8 species of coccidia were identified in cattle in the valley, and 14 genera of helminths and 8 species of coccidia were identified in sheep. In conjunction with the survey, meteorologic studies were conducted May through September, 1909, to ascertain the effects which irrigation had upon micro-environments on grass pastures in relation to the development of free-Iiving stages of gastro-intestinal nematodes of ruminants. The …

The Incidence And Intensity Of Haematozoa In The Common Crow (Corvus Brachyrhynchos) Of Western Washington, Ronald E. Scarvie

All Master's Theses

The purpose of this study was to determine the incidence and intensity of blood parasites in the common crow (Corvus brachyrhynchos). The following observations on the protozoan genera Haemoproteus, Leucocytozoon, Plasmodium and Trypanosoma with observations on the nematode group microfilaria, are based entirely on materials collected at Auburn, Washington during the spring of 1970.

Despite the numerous studies on prevalence of blood parasites in various birds, there have been few attempts to study the relative incidence and intensity of haematozoa in blood and tissue smears taken from the common crow. So far as the author has been able to ascertain, …

The Effect Of Gamma Irradiation On Growth Of Seven Strains Of Trypanosoma Avium, Roy Wesley Leid Jr.

All Master's Theses

The prime motivating factor in the present study was the desire to show significant differences in radio-sensitivities within six strains of Trypanosoma avium. A seventh strain was later added for the last two irradiation treatments. Gross morphology and differences in growth curves associated with varying temperatures suggested that the strains being cultured were not the same. With these differences in mind, the strains were subjected to different dosage levels of gamma irradiation.

Aspects Of The Ecology, Life History, And Host-Parasite Relationship Of Loxothylacus Panopaei (Sacculinidae) In Chesapeake Bay, Seth Jerome Daugherty

Dissertations, Theses, and Masters Projects

No abstract provided.

Concurrent Infections Of Schistosoma Mansoni And Trypanosoma Equiperdum In Mice, Venus Ee-Siriporn

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this investigation was to determine factors which may influence the survival time of mice infected with Trypanosoma equiperdum and the effects of T. equiperdum on mice infected with Schistosoma mansoni.

In order to maintain experimentally obtained standard hours of survival (88 hrs) in mice inoculated intraperitoneally with 150,000 T. equiperdum, it was found that trypanosomes removed from the donor rat should be kept at 25 C and all counts, dilutions of the organisms, and injection should be completed within 1 hour after removal from the donor host. Survival time of mice is increased as the …

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

Studies On Blood Parasites Of Birds In Coles County, Illinois, Edward G. Fox

Masters Theses

No abstract provided.

Some Endoparasites Of The Herpetofauna Of Dominica, West Indies, And Their Use As Clues To Host Zoogeography, Eugene William Nicholls

Dissertations, Theses, and Masters Projects

No abstract provided.

Carbon Dioxide Fixation In Schistosoma Mansoni, Clint Earl Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined …

The Effect Of Schistosoma Mansoni On Free Amino Acid Levels In Mice, Richard D. Tkachuck

Loma Linda University Electronic Theses, Dissertations & Projects

Free amino acid levels in the blood plasma of mice infected with Schistosoma mansoni Sambon, 1907, were measured at 3, 6, and 9 weeks post-infection with an automatic amino acid analyzer. Significant differences between experimental and control animals occurred at all three time periods. At 3 weeks, asparagine, alanine, citrulline, proline, methionine, and threonine levels all were lower in the infected animals. At 6 weeks, four differences were noted. Alpha-amino-n-butyric acid, ornithine, lysine, and histidine all had lower values in infected animals. At 9 weeks there were 12 differences. Tyrosine, phenylalanine, threonine, serine, proline, citrulline, glycine, alanine, valine, isoleucine, and …

Ticks Of The Genus Haemaphysalis In The Western United States, Joe M. Merino

Theses and Dissertations

Two species of Haemaphysalis occurring in the western United States were studied with respect to geographical distribution, seasonal occurrence, host-parasite association and external anatomy and morphology. H. leporispalustris is widely distributed in the west. Abundant records for some states are lacking and should be provided to extend the knowledge of this tick's distribution.

Dermacentor Andersoni And Rocky Mountain Spotted Fever In National Forest Recreational Sites Of Utah, C. Selby Herrin

Theses and Dissertations

The objectives of this study were to determine (1) the prevalence of adult ticks of Dermacentor andersoni in national forest recreational sites of Utah, and (2) the incidence of spotted fever rickettsia, Rickettsia rickettsii, in the ticks of these areas. With the use of a white flannel cloth, 358 adult D. andersoni (135 males and 223 females) were collected from 48 recreational sites during the spring and summer of 1964. Ticks from each collection were put in pools, preserved in non-fat skim milk at -30° C, and subsequently tested for the presence of spotted fever rickettsia by guinea pig inoculations. …

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Life History Of A Haemogregarine, Hepatozoon Sp. In The Chuckwalla: Sauromalus, John E. Lewis

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]

Quantitative Evaluation Of Streptococcal Antibodies By A Serum Inhibition Fluorescent Antibody Tube Method, Charles Glenn Nichols

Loma Linda University Electronic Theses, Dissertations & Projects

The development and description of a quantitative method of evaluating streptococcal antibodies by inhibition of fluorescent staining is reported. Suspensions of Streptococcus pyogenes group A, treated with methyl alcohol to reduce auto-fluorescence, were used as antigen. The degree of inhibition of uptake of fluorescent labeled antibody produced by the presence of antibodies in the immune test sera was measured after elution of the labeled antibody. Comparisons were made with controls using normal sera containing no inhibiting antibodies. A method of reporting the results in terms of titer is described.

Streptococcal antibodies were demonstrated quantitatively by this inhibition test in sera …

Rapid Identification Of Streptococcal Infections Using Fluorescent Antibody Techniques, James H. Meadows

Biology ETDs

The use of fluorescent microscopy coupled with immunological techniques offer new methods for the demonstration of antigen-antibody reactions. The adaptation of these techniques to grouping of beta hemolytic streptococci makes feasible their further adaptation toward identification of group A streptococci in clinical material. Commercially available labeled antibodies against group A beta hemolytic streptococci were obtained for use in this study. A method for the determination of labeled antisera specificity is proposed and a technique for absorbing out any heterologous reacting antibody is described. Using this commercial labeled antisera, subsequent to specificity studies plus absorption if necessary, adaptations of basic fluorescent …

A Morphological Study Of Some Larval Ticks Of The Genus Dermacentor With Emphasis On Structures Of Possible Taxonomic Importance, Richard Grant Robertson

Theses and Dissertations

This study was undertaken to evaluate and determine morphological characteristics which might prove valuable in the identification of the larvae of the following species of ticks of the genus Dermacentor Koch: D. albipictus Packard, D. andersoni Stiles, D. hunteri Bishop, D. parumapertus Neuman, and D. variabilis Say. A taxonomic key for the identification of all of the Canadian and United States species of the larvae of the genus Dermacentor Koch cannot be constructed until specimens of D. halli and D. occidentalis have been examined. However, the followng conclusions were reached regarding the species studied: 1. Position of setae is of …

The Effect Of The Shave On Infection In Maternity Patients, Doris Christine Bethea

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this study was to find out if the shave routinely received by maternity patients could be limited to the perineal area without increasing the incidence of infection among these patients. The experimental method, with the parallel group technique and a single variable, was used.

The study was conducted at two hospitals; the White Memorial Hospital in Los Angeles, California, called Hospital A; and the Washington Sanitarium and Hospital in Takoma Park, Maryland, referred to as Hospital B. Data were collected on ninety clinic patients at Hospital A and on two-hundred and one private patients at Hospital B. …

Identification Of Larval Ticks Of The Genus Ixodes Known To Occur In Utah, Leland D. White

Theses and Dissertations

The purpose of this study is to present an analysis and key to the larvae of the nine species of Ixodes known to occur in Utah. These are I. kingi, I. muris, I. texanus, I. pacificus, I. spinipalpis, I. marmotae, I. ochotonoe, I angustus, and I. sculptus. Larvae from the first five of these nine species used in this study were reared in the laboratory by worked cited in the secion on Analysis of Species. those of the remaining four were identified on the basis of their presence with identifiable numphal and adult forms. Host specificity, although certainly variable with …

Natural History Of Thelazia Californiensis Price, 1930 (Nematoda: Thelaziidae), A Mammalian Eye Worm, Warren E. Parmelee

Loma Linda University Electronic Theses, Dissertations & Projects

Various factors—embryology, host associations, and seasonal changes In eye worn populations—tending to promote the propagation of T. califomlensls were studied.

The early embryology closely parallels that of other nematode species.

The mature embryos remain alive in saline at 35°C as long as 5 days. Dead embryos can be distinguished from living ones by virtue of the rapid staining by methylene blue by the former. Parturient female thelazias release embryos into saline, and embryos are found regularly in tear secretions of infected laboratory rabbits.

Ovic embryos possess an enveloping egg membrane containing chitin. On this account, this eye worm species is …

Natural And Experimental Infections Of Thelazia Californiensis Price (1930, Nematoda: Thelaziidae) With Descriptions Of The Adult And Larval Parasites, Howard S. Burnett

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this work was to determine the extent and effects of infestation of the eye worm of mammals, Thelazia californiensis, and to elucidate through experimentation or observation the mode of transmission.

A survey of literature revealed that thelaziasie (infestation by Thelazia species) may cause serious pathological changes in the eye and its adnexa, sometimes resulting in blindness. It was found that reports of thelaziasis in the United States were (with two exceptions) of cases in California. It was further found that T. californiensis has been reported in a wider variety of host than has any of other …

Oviposition Habits Of The Tick Dermacentor Parumapertus Neumann And Factors Influencing Egg Development, Clive D. Jorgensen

Theses and Dissertations

This paper includes data concerning the influence of relative humidity, temperature, and percentage of engorgement on the pre-oviposition period, oviposition period, post-oviposition period, and incubation period of the tick Dermacentor parumapertus Neumann. The influence of the percentage of engorgement on the oviposition period, oviposition rate, and egg number is considered. Data on the disposition and number of eggs in nature are discussed.

Evidence Against The Transmission Of Staphylococci By The Mosquito Culex Tarsalis, John R. Molenda

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Staphylococcal synovitis is a pathological condition of birds involving primarily the wing and leg joints. It is frequently referred to as arthritis, osteoarthritis, bursitis, hock disease, staphylococcosis, and weak leg.

The disease has been reported throughout various parts of the world as having been found in a great variety of avian hosts including pheasants, chickens, pigeons, canaries, geese, ducks, grouse, and turkeys.

Miner (1945) presented results of a survey made on turkey diseases in Sanpete County, Utah, which indicated staphylococcal synovitis to be responsible for the greatest numbers of deaths. His report cites this infection as having been found in …