Life Sciences Commons^™

Parasites From Gizzard Shad, Dorosoma Cepedianum, In Lake Charleston, Illinois, Francis M. Yabai Kaikumba

Masters Theses

Eighty gizzard shad (Dorosoma cepedianum) from Lake Charleston, Charleston, Illinois were examined for parasites. Eight fish were parasitized. Three fish contained the acanthocephalan Tanaorhamphus longirostris, three fish harbored cestodes of the genus Glaridacris and two fish had the nematode Camallanus oxycephalus. The latter is here reported for the first time from gizzard shad. Twenty fish were infected with the fungus Saprolegnia sp. The numbers of helminth parasites harbored by shad are less than reported for other forage fish. However, the incident of parasitism reported in this study is greater than that reported by previous workers.

The Effects Of Different Gases On Excystation Of Coccidian Oocysts, James B. Jensen

Theses and Dissertations

Excystation of coccidian oocysts is a diphasic process where CO2 and suitable reducing agents cause changes in oocyst wall permeability (phase I) allowing the entrance of trypsin and bile which activate the sporozoites (phase II). This project was an indirect study of the mechanism of CO2 action by the substitution of NO, NO2, N2O, H2S, SO2 , CH4, NH3 and 8M urea in place of CO2. Changes in oocyst wall permeability of Eimeria stiedae, E. bovis and E. tenella were determined by incubation with the reagents and cysteine HCl followed by treatment with trypsin and bile to initiate activation of …

The Effects Of Temperatures And Chemicals On The Oocysts Of Eimeria Stiedae, Charles Larry Keehner

Theses and Dissertations

A study was made of the effects of temperatures and chemicals on the unsporulated and sporulated oocysts of Eimeria stiedae. In general, oocysts were affected by temperatures lower than -10 C and above +39 C, while normal development occurred between these temperatures. Unsporulated oocysts were more tolerant to temperature extremes, as they survived up to 8 days at 39 C and 12 days at -28 C. Sporulated oocysts were killed after 24 hours storage at 39 C and 4 days at -28 C. Oocyst resistance to low temperatures was not improved if unsporulated and sporulated oocysts were conditioned before and …

The Comparative Histochemistry Of The Infective Oncospheres Of Oochoristica Osheroffi Meggitt, 1934, And Oochoristica Scelopori Voge And Fox, 1950, (Cyclophyllidea: Linstowiidae) With Notes On The Origins Of The Egg Membranes, Gary R. Coulter

Loma Linda University Electronic Theses, Dissertations & Projects

Infective oncospheres of Oochorlstica osheroffi and Oochoristica scelopori were compared using fifteen histochemical tests. Vital stains were also used.

Carnoy’s-fixed gravid proglottids were vacuum-embedded in a paraffin-piccolyte mixture and cut transversely at 6 microns. Sections were tested for the presence of phosphatases, proteins, sclerotin precursors, nucleic acids, carbohydrates and acid mucopolysaccharides.

Oncospheres were histochemlcally very similar. Heavy concentrations of mucoproteins and glycoproteins were detected in the oncospheral coat of O. scelopori. Acid phosphatase was found in the embryophore of O. osheroffi, but could not be demonstrated in O. scelopori. No evidence of a sclerotized egg capsule was …

Studies On The Regulation Of Cell Growth And Contact Inhibition Using Concanavalin A And Dibutyryl Adenosine Cyclic Monophosphate, Robert W. Teel

Loma Linda University Electronic Theses, Dissertations & Projects

Both dibutyryl cyclic AMP and trypsinized concanavalin A have been shown to inhibit growth in certain cell lines but little testing of these substances has been done using human tumor cell lines. Further, there are at this time no published experimental attempts to correlate the effects on growth with a particular phase of the cell cycle in any cell line.

My experimental data show that the human tumor cell lines HeLa, HEp 2 and KB exhibit pronounced inhibition of growth when treated with trypsinized concanavalin A and reach a contact-inhibited state at a density far below that to which these …

The Reproductive Biology Of The Leech Helobdella Stagnalis (Linnaeus) In Utah Lake, David L. Tillman

Theses and Dissertations

The reproductive biology of the brooding leech Helobdella stagnalis (Linnaeus) in Utah Lake was studied for an 18 month period from June 1970 to December 1971. In determining the annual reproductive cycle, the number of adult leeches carrying eggs or young were counted at weekly intervals during the reproductive season and monthly during the rest of the year. Gonadal staging data from histological sections were correlated with the field collection data. It was found that two broods of young were produced by the adult animals during the reproductive season. Continuous water temperatures were recorded at the study site throughout the …

The Life Cycle Of Lissorchis Heterorchis, Macy And Krygier (1969), Brendan P. H. Onyejekwe

Dissertations and Theses

Many Flumenicola virens (Lea) collected from Crystal Springs Creek and Tualatin River, Portland, Oregon, were found to shed large numbers of a species of microcercous cercariae containing refractile granules. They resembled the cercariae of Triganodistomum mutabile (=Lissorchis mutabile Cort, 1918 as described by Wallace (1939), and subsequent experiments proved that they were the larval stages of what was described by Macy and Krygier (1969), as Lissorchis heterorchis. Encystment of these cercariae was induced experimentally in uninfected adult brown planarians collected partly from Crystal Springs Creek and partly from Carolina Biological Research Station in Gladstone, Portland, Oregon. Adult flukes were obtained …

Ectoparasites Of Fishes Of The Antarctic Peninsular Area, Ervin Lynn Suydam

Dissertations, Theses, and Masters Projects

No abstract provided.

Gastro-Intestinal And Abdominal Helminths Of White-Tailed Deer (Odocoileus Virginianus) In Southern Illinois, Thomas W. Cook

Masters Theses

In November and December 1971, forty deer from five southern Illinois counties were examined by necropsy for gastro-intestinal and abdominal helminths. Eight species of gastro-intestinal nematodes were found: Gongylonema pulchrum, Spiculopteroides odocoilei, Ostertagia mossi, Haemonchus contortus, Cooperia sp., Capillaria sp., Trichuris ovis and Oesophagostomum venulosum. Setaria yehi, a filiariad nematode, was found in the abdominal cavity. Moniezia benedeni was the only cestode found. No trematodes were recovered.

Gastro-Intestinal Helminths Of Illinois Bobwhites, Michael Eugene Gordon

Masters Theses

The viscera of 103 bobwhite quail (Colinus virginianus), from east central Illinois counties, were collected during November and December of 1971 and examined for helminth parasites. Two nematodes were identified: Dispharynx nasuta (9.7%) and Ascaridia sp. (7.8%). The rate of infection varied significantly between counties but there was no difference in parasitism of males and females.

Post-Mortem Examination Of Cattle And Sheep For Parasitic Helminths, Phil Duke Wright

Theses and Dissertations

A survey of helminth parasites of sheep and cattle was conducted to determine the incidence and distribution of these parasites that could be detected by post-mortem examination. Animals were obtained from local abattoirs within the valley, and the viscera were examined for the presence of helminth parasites. Parasites identifies from sheep in order of highest incidence were: Haemonchus contorus, Ostertagia cicumcinta, Chabertia ovina, Nematodirus spathiger, Trichostongylus colubriformis, Thysanosoma actinioides, Trichuris ovis, N. filicollis, Echinococcus granulosus, Strongyloides papillosus, O. ostertagi, Cooperia oncophora, Cysticercus tenuicollis, Fasciola hepatica, Oesophagostomum venulosum, Moniezia expansa, and M. benedieni. Those recovered from cattle in order of highest …

The Effect Of Elevated Glucose On Neutrophilic Phagocytosis In Genetically Diabetic Mice, Larry B. Thompson

Loma Linda University Electronic Theses, Dissertations & Projects

Infiltration to an injury site or area of bacterial contamination by polymorphonuclear leucocytes (or neutrophils) is an early and essential phase of the early inflammatory reaction. Their action on foreign matter is the most critical step taken by body defense mechanisms in the first 24 hours. Anything which interferes with that action, phagocytosis, greatly hampers early inflammation. This study was designed to see the effect of elevated glucose levels on neutrophil phagocytosis in a unique strain of genetically diabetic mice.

Twenty-two diabetic and twenty-four control female mice were used. Blood glucose determinations and total white counts were made on each …

Effect Of Splenectomy On Relapse Of Hemal Parasites In Pigeons, Phillip James Sprino

Masters Theses

No abstract provided.

Protozoan And Helminth Parasites Of White-Tailed Deer (Odocoileus Virginianus) In Southern Illinois, Sarah M. Mugwanya

Masters Theses

No abstract provided.

The Effect Of The Parasite Hymenolepis Diminuta On Iodine Economy In The Rat, David Gourdon Holmes

Theses and Dissertations

A balance of iodine within the rats is important for the maintenance of normal thyroid function. Because of Hymenolepis d[imi]nuta's affinity for certain nutrients, it may upset norma[l] iodine balance in the rat. To determine this, tapeworm-infected and non-infected rats were given 125Iodide orally and the percent uptake of iodide by the thyroid and the amount of radioac[tiv]ity in the tapeworms was determined. The percent distribution of the radio-iodinated compounds in the thyroid, tapeworms and feces was also determined. These procedures were repeated except 125Thyroxine was administered orally. There was a decrease in percent iod[id]e uptake by thyroids of tapeworm-infected …

Infectivity And Development Of Trichinella Spiralis In Germ-Free, Monocontaminated And Conventional Mice, Daulat Sitanggang

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of the experiment was to compare the development of Trichinella spiralis in germ-free, Lactobacillus casei monocontaminated, Escherichia coli monocontaminated and conventional mice. The criteria used in the experiments were the recovery of adults and larvae of T. spiralis after 72 hours and one month post-infection, respectively. which gave an indication of the effects of no bacteria or only a certain, single species of bacterium on the development of this nematode in mice.

Germ-free T. spiralis larvae were obtained by washing them several times with sterile normal saline, and treatment with antibiotics. The gavaging procedure was done without anesthetization …

A Survey Of The Helminth Parasites And Coccidia Of Cattle And Sheep In Utah Valley, Joseph Carl Fox

Theses and Dissertations

A survey of gastro-intestinal parasites in cattle and sheep in Utah Valley was conducted. Cattle in the valley were not seriously parasitized by helminths, whereas sheep usually had heavy helminth infections. Ten genera of helminths and 8 species of coccidia were identified in cattle in the valley, and 14 genera of helminths and 8 species of coccidia were identified in sheep. In conjunction with the survey, meteorologic studies were conducted May through September, 1909, to ascertain the effects which irrigation had upon micro-environments on grass pastures in relation to the development of free-Iiving stages of gastro-intestinal nematodes of ruminants. The …

The Incidence And Intensity Of Haematozoa In The Common Crow (Corvus Brachyrhynchos) Of Western Washington, Ronald E. Scarvie

All Master's Theses

The purpose of this study was to determine the incidence and intensity of blood parasites in the common crow (Corvus brachyrhynchos). The following observations on the protozoan genera Haemoproteus, Leucocytozoon, Plasmodium and Trypanosoma with observations on the nematode group microfilaria, are based entirely on materials collected at Auburn, Washington during the spring of 1970.

Despite the numerous studies on prevalence of blood parasites in various birds, there have been few attempts to study the relative incidence and intensity of haematozoa in blood and tissue smears taken from the common crow. So far as the author has been able to ascertain, …

The Effect Of Gamma Irradiation On Growth Of Seven Strains Of Trypanosoma Avium, Roy Wesley Leid Jr.

All Master's Theses

The prime motivating factor in the present study was the desire to show significant differences in radio-sensitivities within six strains of Trypanosoma avium. A seventh strain was later added for the last two irradiation treatments. Gross morphology and differences in growth curves associated with varying temperatures suggested that the strains being cultured were not the same. With these differences in mind, the strains were subjected to different dosage levels of gamma irradiation.

Aspects Of The Ecology, Life History, And Host-Parasite Relationship Of Loxothylacus Panopaei (Sacculinidae) In Chesapeake Bay, Seth Jerome Daugherty

Dissertations, Theses, and Masters Projects

No abstract provided.

Some Aspects Of Carbohydrate Metabolism In The Cercariae Of Schistosoma Mansoni Sambon, 1907, A. Eugene Dunham Jr.

Loma Linda University Electronic Theses, Dissertations & Projects

Cercariac of Schistosoma mansoni were studied for glycogen utilization, uptake of glucose from solution, lactic acid production, and respiration.

Using the anthrone method of determination of carbohydrate, the amount of glycogen used by a single cercaria in 4 hours is determined as 43.2 x 10^-4 μg ± 6.6 x 10^-4 μg.

Glucose was not observed to have been used from solution under the conditions of the experiment, even in the presence of trehalose. The determinations were made by the Glucostat method.

After deproteinization and desugarization, aliquots of supernatant from cercarial suspensions were analyzed for lactic acid using p-phenylphenol …

Concurrent Infections Of Schistosoma Mansoni And Trypanosoma Equiperdum In Mice, Venus Ee-Siriporn

Loma Linda University Electronic Theses, Dissertations & Projects

The purpose of this investigation was to determine factors which may influence the survival time of mice infected with Trypanosoma equiperdum and the effects of T. equiperdum on mice infected with Schistosoma mansoni.

In order to maintain experimentally obtained standard hours of survival (88 hrs) in mice inoculated intraperitoneally with 150,000 T. equiperdum, it was found that trypanosomes removed from the donor rat should be kept at 25 C and all counts, dilutions of the organisms, and injection should be completed within 1 hour after removal from the donor host. Survival time of mice is increased as the …

Some Endoparasites Of The Herpetofauna Of Dominica, West Indies, And Their Use As Clues To Host Zoogeography, Eugene William Nicholls

Dissertations, Theses, and Masters Projects

No abstract provided.

Studies On Blood Parasites Of Birds In Coles County, Illinois, Edward G. Fox

Masters Theses

No abstract provided.

Carbon Dioxide Fixation In Schistosoma Mansoni, Clint Earl Carter

Loma Linda University Electronic Theses, Dissertations & Projects

Adults of the blood fluke Schistosoma mansoni were perfused from the hepatic portal system of CF1 female mice sixty to seventy days post infection. The worms were then incubated in Eagle’s minimal essential medium with glutamate and 0.03 mg/100 ml of nonlabelled aspartic acid. To this medium was added 10 μc of either NaH¹⁴0₃, glucose-UC¹⁴ or aspartate-4-C¹⁴. The worms were found to fix carbon from the NaH¹⁴0₃ and to incorporate the glucose and aspartate. The amount of activity recovered in five different chemical fractions after two hours incubation was determined …

The Effect Of Schistosoma Mansoni On Free Amino Acid Levels In Mice, Richard D. Tkachuck

Loma Linda University Electronic Theses, Dissertations & Projects

Free amino acid levels in the blood plasma of mice infected with Schistosoma mansoni Sambon, 1907, were measured at 3, 6, and 9 weeks post-infection with an automatic amino acid analyzer. Significant differences between experimental and control animals occurred at all three time periods. At 3 weeks, asparagine, alanine, citrulline, proline, methionine, and threonine levels all were lower in the infected animals. At 6 weeks, four differences were noted. Alpha-amino-n-butyric acid, ornithine, lysine, and histidine all had lower values in infected animals. At 9 weeks there were 12 differences. Tyrosine, phenylalanine, threonine, serine, proline, citrulline, glycine, alanine, valine, isoleucine, and …

Ticks Of The Genus Haemaphysalis In The Western United States, Joe M. Merino

Theses and Dissertations

Two species of Haemaphysalis occurring in the western United States were studied with respect to geographical distribution, seasonal occurrence, host-parasite association and external anatomy and morphology. H. leporispalustris is widely distributed in the west. Abundant records for some states are lacking and should be provided to extend the knowledge of this tick's distribution.

Dermacentor Andersoni And Rocky Mountain Spotted Fever In National Forest Recreational Sites Of Utah, C. Selby Herrin

Theses and Dissertations

The objectives of this study were to determine (1) the prevalence of adult ticks of Dermacentor andersoni in national forest recreational sites of Utah, and (2) the incidence of spotted fever rickettsia, Rickettsia rickettsii, in the ticks of these areas. With the use of a white flannel cloth, 358 adult D. andersoni (135 males and 223 females) were collected from 48 recreational sites during the spring and summer of 1964. Ticks from each collection were put in pools, preserved in non-fat skim milk at -30° C, and subsequently tested for the presence of spotted fever rickettsia by guinea pig inoculations. …

The Effect Of Varying Protein Intake On Trichinella Spiralis Infection In Mice, Clare K. Kwan

Loma Linda University Electronic Theses, Dissertations & Projects

Three hundred Swiss Webster albino mice three weeks of age were placed on low (8%), medium (24%), high (48%) protein levels and a commercial laboratory animal feed, Purina Chow for various lengths of time: (1) simultaneously with infection, and four weeks thereafter, (2) three weeks before infection and throughout the four-week infection period, and (3) six weeks prior to infection and during the infection period. The protein source was casein. Sixty of the 300 mice used served as noninfected controls, and the other 240 were each infected with 400 washed, suspended Trichinella spiralis larvae obtained from a rat by the …

Life History Of A Haemogregarine, Hepatozoon Sp. In The Chuckwalla: Sauromalus, John E. Lewis

Loma Linda University Electronic Theses, Dissertations & Projects

[Abstract Not Included]