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Articles 61 - 90 of 155
Full-Text Articles in Life Sciences
Possible Distorting Effects Of Gef26 In Drosophila Melanogaster, Kaylie C. Church
Possible Distorting Effects Of Gef26 In Drosophila Melanogaster, Kaylie C. Church
Honors Program Projects
Segregation distortion is a meiotic drive system that results in the favorable inheritance of the SD chromosome over the SD+counterpart. SD produces a malfunctioning form of RanGAP, instead of being able to move in and out of the nucleus this truncated anGAP is unable to be exported and therefore aggregates within the nucleus. This appears to affect RspS during the condensation phase of spermatogenesis, but the exact mechanism for this is unknown. In order to further understand the working components of this system, specific deletions within the second chromosome were studied. The study was conducted systematically by beginning with …
Molecular Marker Assisted Development And Stability Analysis Of Low Phytate, High Inorganic Phosphorus Soybean [Glycine Max (L.) Merrill], Suzannah Joy Wiggins
Molecular Marker Assisted Development And Stability Analysis Of Low Phytate, High Inorganic Phosphorus Soybean [Glycine Max (L.) Merrill], Suzannah Joy Wiggins
Masters Theses
Phytate [myo-inositol 1,2,3,4,5,6-hexakisphosphate] is a mixed cation salt of phytic acid which binds to other minerals, in many field crops including soybean [Glycine max (L.) Merr.]. Livestock with monogastric digestive systems lack the ability to break bonds between phytate and these minerals, causing phytate phosphorus (P) to be excreted in the waste and contributing to possible P and other mineral deficiencies. Discovery of single sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers associated with low phytate QTL (cqPha-001 and cqPha-002) have aided in development of low phytate soybean lines. The objectives of this study were …
Predictors Of Contralateral Breast Cancer In Brca Negative Women, Ann E. Simmons
Predictors Of Contralateral Breast Cancer In Brca Negative Women, Ann E. Simmons
Dissertations & Theses (Open Access)
Breast cancer is the most common cancer diagnosis and second leading cause of death in women. Risk factors associated with breast cancer include: increased age, alcohol consumption, cigarette smoking, white race, physical inactivity, benign breast conditions, reproductive and hormonal factors, dietary factors, and family history. Hereditary breast and ovarian cancer syndrome (HBOC) is caused by mutations in the BRCA1 and BRCA2 genes. Women carrying a mutation in these genes are at an increased risk to develop a second breast cancer. Contralateral breast cancer is the most common second primary cancer in patients treated for a first breast cancer. Other risk …
Diversity, Identification, Mapping And Pyramiding Of Genes For Resistance To Soybean Mosaic Virus [Glycine Max (L.) Merr.], Innan Gloria Cervantes Martinez
Diversity, Identification, Mapping And Pyramiding Of Genes For Resistance To Soybean Mosaic Virus [Glycine Max (L.) Merr.], Innan Gloria Cervantes Martinez
Graduate Theses and Dissertations
Soybean Mosaic Virus (SMV) is a prevalent viral pathogen transmitted by aphids and via seed, causing significant yield loss and quality reduction. There are seven SMV strain groups (G1 to G7) and three resistance loci( Rsv1, Rsv3, and Rsv4) reported in soybean. New sources of SMV resistance would be valuable for breeding cultivars with durable resistance to multiple strains. The objectives of this research were to analyze genetic diversity of SMV-resistant soybean germplasm at the molecular level; to pyramid three genes from different sources for durable SMV resistance; and to identify and map new genes/alleles for differential reactions to SMV …
Knowledge, Attitudes, And Utilization Of Brca Testing Among Obstetricians And Gynecologists, Salma Nassef
Knowledge, Attitudes, And Utilization Of Brca Testing Among Obstetricians And Gynecologists, Salma Nassef
Dissertations & Theses (Open Access)
Hereditary breast and ovarian cancer (HBOC) is an inherited cancer syndrome that is associated with mutations in the BRCA1 and BRCA2 genes. Carriers of BRCA mutations, both men and women, are at an increased risk for developing certain cancers. Carriers are most notably at an increased risk to develop breast and ovarian cancers; however an increased risk for prostate cancer, melanoma, and pancreatic cancers has also been associated with these mutations. In 2009 the American Congress of Obstetricians and Gynecologists (ACOG) released a practice bulletin stating that evaluating a patient’s risk for HBOC should be a routine part of obstetric …
Genetic Predictors Of Hyperglycemia Due To Hydrochlorothiazide Therapy, Jorge L. Del Aguila
Genetic Predictors Of Hyperglycemia Due To Hydrochlorothiazide Therapy, Jorge L. Del Aguila
Dissertations & Theses (Open Access)
Response to pharmacological treatment is variable among individuals. Some patients respond favorably to a drug while others develop adverse reactions. Early investigations showed evidence of variation in genes that code for drug receptors, drug transporters, and drug metabolizing enzymes; and pharmacogenetics appeared as the science that studies the relationship between drug response and genetic variation.
Thiazide diuretics are the recommended first-line monotherapy for hypertension (i.e. SBP>140 or DBP>90). Even so, diuretics are associated with adverse metabolic side effects, such as hyperglycemia, which increase the risk of developing type 2 diabetes. Published approaches testing variation in candidate genes (e.g. …
Effects Of Thymus Size And Involution On The Contribution Of Recent Thymic Emigrants To The Peripheral T Cell Pool, Michelle L. Bolner
Effects Of Thymus Size And Involution On The Contribution Of Recent Thymic Emigrants To The Peripheral T Cell Pool, Michelle L. Bolner
Dissertations & Theses (Open Access)
The contribution of recent thymic emigrants (RTEs) to the peripheral naïve T cell population is necessary to maintain diversity of the T cell receptor (TCR) repertoire and produce immune responses against newly encountered antigens.
The thymus involutes with age, after irradiation or chemotherapy, and due to severe viral infections. Thymus involution results in decreased thymopoiesis and RTE output leading to a reduced diversity of peripheral T cells. This increases susceptibility to disease and impairs immune responsiveness to vaccines. Therefore, studies aimed at maintaining or regenerating thymic function are integral for maintaining and restoring peripheral TCR diversity.
Mice that express a …
Abd-A Regulation By The Iab-8 Noncoding Rna, Maheshwar Gummalla, Robert K. Maeda, Javier J. Castro Alvarez, Henrik Gyurkovics, Kevin A. Edwards, Swetha Singari, Welcome Bender
Abd-A Regulation By The Iab-8 Noncoding Rna, Maheshwar Gummalla, Robert K. Maeda, Javier J. Castro Alvarez, Henrik Gyurkovics, Kevin A. Edwards, Swetha Singari, Welcome Bender
Faculty Publications – Biological Sciences
The homeotic genes in Drosophila melanogaster are aligned on the chromosome in the order of the body segments that they affect. The genes affecting the more posterior segments repress the more anterior genes. This posterior dominance rule must be qualified in the case of abdominal-A (abd-A) repression by Abdominal-B (Abd-B). Animals lacking Abd-B show ectopic expression of abd-A in the epidermis of the eighth abdominal segment, but not in the central nervous system. Repression in these neuronal cells is accomplished by a 92 kb noncoding RNA. This "iab-8 RNA" produces a micro RNA to repress abd-A, but also has a …
Signaling Pathways And Genetic Interactions That Lead To Metastatic Cancer In Drosophila Melanogaster, Amanda Cole
Signaling Pathways And Genetic Interactions That Lead To Metastatic Cancer In Drosophila Melanogaster, Amanda Cole
Honors Capstone Projects - All
Cancer is a complex and multigenic disease, which is typically initiated by genetic mutations in tumor suppressor genes that regulate homeostatic mechanisms within cells. Oncogenic promoter mutations, like those involved in signal transduction pathways, also have the potential to induce cancer in an otherwise healthy organism. Transformation is highly dependent upon mutations to both tumor suppressor and oncogenes, as neither mutation is exclusive in its ability to generate malignant tumors. In the model organism, Drosophila melanogaster, I have generated metastatic cancer through the genetic effect of overactive Raf signaling, in conjugation with silencing selected tumor suppressor genes using RNA …
Heterotopic Ossification: Cellular Basis, Symptoms, And Treatment, Brian Wolfe
Heterotopic Ossification: Cellular Basis, Symptoms, And Treatment, Brian Wolfe
Senior Honors Theses
Heterotopic ossification (HO) is the process by which calcified bone develops in soft tissues. Because of the abnormal calcification, complications such as bone deformation, loss of range of motion, and joint immobility adversely affect patients. There are many genetic types of heterotopic ossification, namely fibrodysplasia ossificans progressiva, progressive osseous heteroplasia, and Albright hereditary osteodystrophy. However, this condition can also arise from surgery, burns, or traumatic injuries, so it is seen as an important area for research in the future. There are various treatments available such as non-steroidal anti-inflammatory drugs and radiation therapy, as well as combinations of the two. The …
Revised Selection Criteria For Candidate Restriction Enzymes In Genome Walking, Ali Taheri, Stephen J. Robinson, Isobel Parkin, Margaret Y. Gruber
Revised Selection Criteria For Candidate Restriction Enzymes In Genome Walking, Ali Taheri, Stephen J. Robinson, Isobel Parkin, Margaret Y. Gruber
Agricultural and Environmental Sciences Faculty Research
A new method to improve the efficiency of flanking sequence identification by genome walking was developed based on an expanded, sequential list of criteria for selecting candidate enzymes, plus several other optimization steps. These criteria include: step (1) initially choosing the most appropriate restriction enzyme according to the average fragment size produced by each enzyme determined using in silico digestion of genomic DNA, step (2) evaluating the in silico frequency of fragment size distribution between individual chromosomes, step (3) selecting those enzymes that generate fragments with the majority between 100 bp and 3,000 bp, step (4) weighing the advantages and …
Regression Approaches For Microarray Data Analysis, Mark R. Segal, Kam D. Dahlquist, Bruce R. Conklin
Regression Approaches For Microarray Data Analysis, Mark R. Segal, Kam D. Dahlquist, Bruce R. Conklin
Mark R Segal
A variety of new procedures have been devised to handle the two sample comparison (e.g., tumor versus normal tissue) of gene expression values as measured with microarrays. Such new methods are required in part because of some defining characteristics of microarray-based studies: (i) the very large number of genes contributing expression measures which far exceeds the number of samples (observations) available, and (ii) the fact that by virtue of pathway/network relationships, the gene expression measures tend to be highly correlated. These concerns are exacerbated in the regression setting, where the objective is to relate gene expression, simultaneously for multiple genes, …
Resource Availability, Mortality And Fertility: A Path Analytic Approach To Global Life History Variation, Mark A. Caudell, Robert J. Quinlan
Resource Availability, Mortality And Fertility: A Path Analytic Approach To Global Life History Variation, Mark A. Caudell, Robert J. Quinlan
Human Biology Open Access Pre-Prints
Humans exhibit considerable diversity in timing and rate of reproduction. Life history theory suggests that ecological cues of resource richness and survival probabilities shape human phenotypes across populations. Populations experiencing high extrinsic mortality due to uncertainty in resources should exhibit faster life histories. Here we use a path analytic approach informed by life history theory to model the multiple pathways between resources, mortality rates, and reproductive behavior in 191 countries. Resources that account for the most variance in population mortality rates are predicted to explain the most variance in total fertility rates. Results indicate that resources (e.g., calories, sanitation, education, …
Studies On Enzyme Functional Evolution In The Sabath Multigene Family Using Phylogenetic And Biochemical Approaches, Ruiqi Huang
Studies On Enzyme Functional Evolution In The Sabath Multigene Family Using Phylogenetic And Biochemical Approaches, Ruiqi Huang
Masters Theses
Gene duplication is believed to be the major source for providing genetic materials for the innovation and diversification of protein functions; natural selection and/or neutral drift then works on these genetic materials to guide their evolutionary directions. Here, I used the salicylic acid/benzoic acid/theobromine (SABATH) multigene family to study how natural selection acted on duplicated genes to prompt functional diversification. Members in this family methylate plant secondary metabolites by transferring the methyl group from S-adenosyl-L-methionine (SAM) to the carboxyl group or ring nitrogen of the substrates. In the Apocynaceae and Solanaceae lineage of this family, I documented three putative gene …
Living In An Age Of Technology: College Students' Perception Of The Role Of Genetic Engineering And Reproductive Technology In Today's Society, Kayla Quadros
Honors Projects
This study examines attitudes about prevalent issues in genetics and reproduction among the college population. Eighteen interviews were conducted with students at Rhode Island College. Respondents were asked questions about their moral standpoints concerning utilizing genetic engineering for disease control and aesthetic purposes, employing assisted reproductive technologies (ARTs), regulation of such procedures, and also about media sources for which they have heard of these topics. Findings suggest that, generally, participants felt that genetic engineering for health issues is permissible, yet reprehensible for the purpose of aesthetically "designing" a baby, though four students (three of which were female) found the enhancement …
Association Among Obesity-Related Anthropometric Phenotypes: Analyzing Genetic And Environmental Contribution, Aline Jelenkovic, Esther Rebato
Association Among Obesity-Related Anthropometric Phenotypes: Analyzing Genetic And Environmental Contribution, Aline Jelenkovic, Esther Rebato
Human Biology Open Access Pre-Prints
Obesity has become a public health and policy problem in many parts of the world. Epidemiological and population studies in this field are usually based on different anthropometric measures, however, common genetic and environmental factors between these phenotypes have been scarcely studied. The objective of this work is to assess the strength of these factors on the covariation among a large set of obesity-related traits. The subject group consisted of 533 nuclear families living in the Greater Bilbao (Spain), and included 1702 individuals aged 2-61 years. Detailed anthropometric measurements (stature, breadths, circumferences and skinfolds) were carried out in each subject. …
Microgeographic Differentiation In Historical Yemen Inferred By Morphometric Distances, Maria Enrica Danubio, Emanuele Sanna, Fabrizio Rufo, Domenico Martorella, Elvira Vecchi, Alfredo Coppa
Microgeographic Differentiation In Historical Yemen Inferred By Morphometric Distances, Maria Enrica Danubio, Emanuele Sanna, Fabrizio Rufo, Domenico Martorella, Elvira Vecchi, Alfredo Coppa
Human Biology Open Access Pre-Prints
This study analysed the variations in space of 8 body dimensions and 11 measures of the head of 1,244 adult Yemenite males, collected in 1933/34 by Coon in Yemen and in Hadhramawt. The aim was to evaluate the presence of geographic microdifferentiation of the populations settled in the different regions of Yemen at the time. Coon sub-divided the sample into 6 geographical areas according to birthplace and ethnicity of the individuals: Tihamah, the Western Mountains, the Central Plateau, the South Coast, the Eastern Mountains and Hadhramawt. The results of ANCOVA (age as covariate) show that the observed differences of all …
The Dietary Isoprenoid Perillyl Alcohol Inhibits Telomerase Activity In Prostate Cancer Cells, Tabetha Sundin
The Dietary Isoprenoid Perillyl Alcohol Inhibits Telomerase Activity In Prostate Cancer Cells, Tabetha Sundin
Theses and Dissertations in Biomedical Sciences
This is the first evidence that a plant-derived compound–perillyl alcohol regulates telomerase activity via the mammalian target of rapamycin (mTOR) pathway in prostate cancer cells. Telomerase–the enzyme responsible for immortalizing cells through telomeric repeats addition–is de-repressed early in an aspiring cancer cell. We hypothesized that perillyl alcohol regulates hTERT (human telomerase reverse transcriptase) at the translational and post-translational levels via its effects on the mTOR pathway. A rapid suppression of telomerase activity was detected in prostate cancer cell lines (PC-3 and DU145) in response to biologically-relevant concentrations and short incubations of perillyl alcohol or the mTOR inhibitor—rapamycin.
Western blot analysis …
The Family Name As Socio-Cultural Feature And Genetic Metaphor: From Concepts To Methods, Pierre Darlu, Gerrit Bloothooft, Alessio Boattini, Leendert Brouwer, Matthijs Brouwer, Guy Brunet, Pascal Chareille, James Cheshire, Richard Coates, Paul Longley, Kathrin DräGer, Bertrand Desjardins, Patrick Hanks, Kees Mandemakers, Pablo Mateos, Davide Pettener, Antonella Useli, Franz Manni
The Family Name As Socio-Cultural Feature And Genetic Metaphor: From Concepts To Methods, Pierre Darlu, Gerrit Bloothooft, Alessio Boattini, Leendert Brouwer, Matthijs Brouwer, Guy Brunet, Pascal Chareille, James Cheshire, Richard Coates, Paul Longley, Kathrin DräGer, Bertrand Desjardins, Patrick Hanks, Kees Mandemakers, Pablo Mateos, Davide Pettener, Antonella Useli, Franz Manni
Human Biology Open Access Pre-Prints
A recent workshop on "Family name between socio-cultural feature and genetic metaphor - From concepts to methods" was held in Paris on the 9th and 10th December 2010, partly sponsored by the Social Science and Humanity Institute (CNRS), and by Human Biology. This workshop was intended to facilitate exchanges on recent questions related to the names of persons and to confront different multidisciplinary approaches in a field of investigation where geneticists and historians, geographers, sociologists and ethnologists have all an active part. Here are the abstracts of some contributions.
Neurodegeneration - A Means To An End, Amit Singh
Neurodegeneration - A Means To An End, Amit Singh
Biology Faculty Publications
Cell death, a global phenomenon found throughout the animal kingdom, is a mechanism to maintain tissue homeostasis and for adaptation to changes in the environment [1,2]. Millions of cells die in our body daily- they succumb to stress and commit suicide by a mechanism referred to as cell death or apoptosis [2-4]. Under normal conditions cells are continuously replaced by new cells from the stemor progenitor- cells. For example, an optimum balance in shedding of dead cells from the skin and their replenishment by new ones maintain our health and hygiene. In this context, apoptosis is a mechanism to eliminate …
Modeling The Dispersion Of Eastern Oyster Larvae (Crassostrea Virginica) And Its Effects On The Movement Of Disease Resistant Genes In The Delaware Bay Estuary, Diego A. Narvaez
Modeling The Dispersion Of Eastern Oyster Larvae (Crassostrea Virginica) And Its Effects On The Movement Of Disease Resistant Genes In The Delaware Bay Estuary, Diego A. Narvaez
OES Theses and Dissertations
This study combines several models to address two primary research questions. How does the interaction of larval biology and environmental variability determine the spatial distribution of oyster larvae in Delaware Bay? What is the role of larval dispersion in the transference of disease-resistant genes? The particle-tracking module in the Regional Ocean Modeling System (ROMS) was converted into an Individual-Based model representing Eastern oyster larvae that has growth and vertical migration. Exchange of larvae between natural oyster reefs was estimated and used in an Individual-Based genetic model that simulates the genetic structure of eastern oysters. Particles were released from a number …
Phylogenetics Of Morus (Moraceae) Inferred From Its And Trnl-Trnf Sequence Data., Madhav Nepal
Phylogenetics Of Morus (Moraceae) Inferred From Its And Trnl-Trnf Sequence Data., Madhav Nepal
Madhav Nepal
Comparative Sequence Analysis Of Fatty Acid Desaturase 2 (Fad2) Cdna Sequences From The Compositae [Asteraceae], Paul Ames Muller Jr.
Comparative Sequence Analysis Of Fatty Acid Desaturase 2 (Fad2) Cdna Sequences From The Compositae [Asteraceae], Paul Ames Muller Jr.
Paul Muller
Fatty acid desaturases are enzymes capable of modifying carbon-carbon bonds within fatty acids. All higher plants contain one or more oleic acid desaturases that catalyze insertion of a double bond between carbons 12 and 13 of oleic acid to produce linoleic acid, an unsaturated fatty acid. However, this family of enzymes may vary in specificity and are responsible for a wide spectrum of unsaturated fatty acids found in nature that play important ecological, physiological, and industrial roles. Here, I examine nucleotide sequence evolution and predict structural changes of translated expressed Fatty Acid Desaturase 2 (FAD2) cDNA sequences in the Compositae …
Dialogues, Dilemmas, And Disclosures: Genomic Research And Incidental Findings, Lynn W. Bush, Karen H. Rothenberg
Dialogues, Dilemmas, And Disclosures: Genomic Research And Incidental Findings, Lynn W. Bush, Karen H. Rothenberg
Faculty Scholarship
No abstract provided.
Spatial Genetic Variation Among Spodoptera Frugiperda (Lepidoptera: Noctuidae) Sampled From The United States, Puerto Rico, Panama, And Argentina, Difabachew K. Belay, Pete L. Clark, Steven R. Skoda, David J. Isenhour, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Spatial Genetic Variation Among Spodoptera Frugiperda (Lepidoptera: Noctuidae) Sampled From The United States, Puerto Rico, Panama, And Argentina, Difabachew K. Belay, Pete L. Clark, Steven R. Skoda, David J. Isenhour, Jaime Molina-Ochoa, Claudia Gianni, John E. Foster
Department of Entomology: Faculty Publications
Spatial genetic variability of the fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), was studied by collecting samples from 31 locations in the United States, Argentina, Panama, and Puerto Rico, and then using amplified fragment length polymorphism to detect genetic variation. Analysis of molecular variance showed significant genetic variation in fall armyworm among all (28%) sample locations and individuals within (71%) sample locations; genetic variation of fall armyworm was minimal between sample locations grouped into regions. The pairwise fixation index (FST) comparisons showed significant genetic differentiation (0.288) among the 31 locations. However, dendrograms of results from cluster …
Mama What Is Celiac, Suad Fahad Alferaih
Mama What Is Celiac, Suad Fahad Alferaih
Dr. Suad Fahad AlFuraih
قصة ماما ماهو السلياك
Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok
Estrogenic Compounds Downstream From Three Small Cities In Eastern Nebraska: Occurrence And Biological Effect, Marlo K. Sellin, Daniel D. Snow, Debbie L. Akerly, Alan S. Kolok
Daniel D Snow
Recent studies have detected estrogenic compounds in surface waters in North America and Europe. Furthermore, the presence of estrogenic compounds in surface waters has been attributed, in some cases, to the discharge of wastewater treatment plant (WWTP) effluent. The primary objective of the current study was to determine if WWTP effluent contributes estrogens to the surface waters of Nebraska. A second objective of this study was to determine if estrogens were found in concentrations sufficient enough to manifest feminizing effects on fish. These objectives were satisfied by deploying polar organic chemical integrative samplers (POCIS) and caged fathead minnows at eight …
Comparison Of Two Gelling Agents For Screwworm (Diptera: Calliphoridae) Larval Diets, David B. Taylor
Comparison Of Two Gelling Agents For Screwworm (Diptera: Calliphoridae) Larval Diets, David B. Taylor
David B. Taylor
A sodium polyacrylamide polyacrylate gelling agent, Water-Lock G-400, and a galactan polysaccharide agent, carrageenan, were compared for solidifying larval diets of the screwworm, Cochliomyia hominivorax (Coquerel). Water-Lock diet produced larger pupae with less larval mortality than did carrageenan diet. Pupae from larvae reared on the Water-Lock diet exhibited higher emergence and produced adults with higher fecundity. Water-Lock diet also produced larger larvae at 56 h after oviposition. Densities in excess of 300 mg of eggs (16,200 eggs) per liter of Water-Lock gelled diet did not increase larval yield but did reduce larval weight and survival.
Mitochondrial Dna Variation Among Muscidifurax Spp. (Hymenoptera: Pteromalidae), Pupal Parasitoids Of Filth Flies (Diptera), David B. Taylor, Richard D. Peterson Ii, Allen L. Szalanski, James J. Petersen
Mitochondrial Dna Variation Among Muscidifurax Spp. (Hymenoptera: Pteromalidae), Pupal Parasitoids Of Filth Flies (Diptera), David B. Taylor, Richard D. Peterson Ii, Allen L. Szalanski, James J. Petersen
David B. Taylor
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and sequencing analyses were used to characterize an amplicon of ~625 bp in 4 of the 5 nominate species of Muscidifurax Girault & Sanders, pupal parasitoids of muscoid flies. A single polymorphic nucleotide site was observed among 2 samples of M. raptor Girault & Sanders. No sequence variation was observed among 3 samples of M. raptorellus Kogan & Legner. The sequence of M. uniraptor Kogan & Legner was identical to that of M. raptorellus. Nucleotide divergence among the Muscidifurax spp. ranged from 0.14 to 0.18 substitutions per nucleotide. Muscidifurax zaraptor Kogan & Legner …
Identification Of Screwworm Species By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
Identification Of Screwworm Species By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
David B. Taylor
Restriction fragment length polymorphisms in polymerase chain reaction amplified fragments (PCR-RFLP) of mitochondrial DNA were used to differentiate species of New World screwworms (Diptera : Calliphoridae) . Twenty-seven restriction enzymes were screened on five regions of mtDNA . Eleven restriction fragment length patterns differentiated New World screwworm, Cochliomyia hominivorax (Coquerel), from secondary screwworm, Cochliomyia macellaria (F.) . Five restriction fragment length patterns were polymorphic in C. hominivorax while all fragment patterns were fixed in C. macellaria. Diagnostic restriction fragment length patterns were used for species diagnosis, whereas intraspecific variable patterns were used to characterize field samples and laboratory strains . …