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Identification Of Screwworm Species By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
Identification Of Screwworm Species By Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
David B. Taylor
Restriction fragment length polymorphisms in polymerase chain reaction amplified fragments (PCR-RFLP) of mitochondrial DNA were used to differentiate species of New World screwworms (Diptera : Calliphoridae) . Twenty-seven restriction enzymes were screened on five regions of mtDNA . Eleven restriction fragment length patterns differentiated New World screwworm, Cochliomyia hominivorax (Coquerel), from secondary screwworm, Cochliomyia macellaria (F.) . Five restriction fragment length patterns were polymorphic in C. hominivorax while all fragment patterns were fixed in C. macellaria. Diagnostic restriction fragment length patterns were used for species diagnosis, whereas intraspecific variable patterns were used to characterize field samples and laboratory strains . …
Mitochondrial Dna Variation In Screwworm, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
Mitochondrial Dna Variation In Screwworm, David B. Taylor, Allen L. Szalanski, Richard D. Peterson Ii
David B. Taylor
Polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) analysis was used to characterize mitochondrial DNA (mtDNA) variation in screwworms, Cochliomyia hominivorax, and secondary screwworm, C. macellaria, from the Caribbean, North America and South America. Four amplicons, totaling 7.1 kb, were analysed with sixteen restriction enzymes. A total of 133 restriction sites was observed in the two species, 104 in C. hominivorax, of which nineteen were variable, and ninety-five in C. macellaria, none of which was variable. Fourteen mtDNA haplotypes were observed among eighteen C. hominivorax examined. Mean divergence between C. hominivorax haplotypes (d) was 0.0064 …
Population Genetics And Gene Variation In Secondary Screwworm (Diptera: Calliphoridae), David Taylor, Richard Peterson Ii
Population Genetics And Gene Variation In Secondary Screwworm (Diptera: Calliphoridae), David Taylor, Richard Peterson Ii
David B. Taylor
Allozyme variation in 4 populations of secondary screwworm, Cochliomyia macellaria (F.), representing North, Central, and South America was examined . Variability was observed in 12 of 13 enzyme loci, and the frequency of the most common allele was <0.95 for 6 loci . Observed and expected heterozygosities were 0.149 and 0.154, respectively. Nei's genetic distances were ≤ 0.001. Goodness-of-fit statistics for Hardy-Weinberg equilibrium and Wright's FIS statistics indicated random mating within populations . Wright's FST statistics and chi-square contingency analyses indicated homogeneous gene frequencies among the populations. These data suggest that C. macellaria is a panmictic species with high levels …