Life Sciences Commons^™

Xenobiotic Response In Drosophila Melanogaster: Sex Dependence Of P450 And Gst Gene Induction, Gaelle Le Goff, Frederique Hilliou, Blair Siegfried, Sam Boundy, Eric Wajnberg, Luc Sofer, Pascaline Audant, Richard H. Ffrench-Constant, Rene Feyereisen

Department of Entomology: Faculty Publications

The effect of xenobiotics (phenobarbital and atrazine) on the expression of Drosophila melanogaster CYP genes encoding cytochromes P450, a gene family generally associated with detoxification, was analyzed by DNA microarray hybridization and verified by real-time RT-PCR in adults of both sexes. Only a small subset of the 86 CYP genes was significantly induced by the xenobiotics. Eleven CYP genes and three glutathione S-transferases (GST) genes were significantly induced by phenobarbital, seven CYP and one GST gene were induced by atrazine. Cyp6d5, Cyp6w1, Cyp12d1 and the ecdysone-inducible Cyp6a2 were induced by both chemicals. The constitutive expression of several of the inducible …

Crop Updates 2005 Oilseeds, John Duff, Douglas Hamilton, Graham Walton, Hasan Zaheer, Neil Harris, Heather Cosgriff, Margaret C. Campbell, Bill Crabtree, R. F. Brennan, M. D. A. Bolland, P. M. Damon, Z. Rengel, Russel Speed, Neil Rothnie, John Simons, Ted Spadeck, John Moore, Brenda Coutts, Roger Jones, Neil Harris, Moin Salam, Ravjit Khangura, Art Diggle

Crop Updates

This session covers fifteen papers from different authors:

1. ACKNOWLEDGEMENTS, Douglas Hamilton, FARMING SYSTEMS DEVELOPMENT OFFICER

CROP AGRONOMY AND NUTRITION

2. Canola workshop at Crop Updates 2005, Oilseeds WA, John Duff, EXECUTIVE OFFICER OILSEEDS WA

3. Comparison of IT and TT canola varieties in geographic zones of WA, 2003-4, Graham Walton and Hasan Zaheer, Department of Agriculture

4. Farmer scale canola variety trials in WA, 2004, Graham Walton, John Duff, Neil Harris and Heather Cosgriff, Oilseeds WA

5. Oilseed crops for industrial uses, Margaret C. Campbell, Centre for Legumes in Mediterranean Agriculture (CLIMA), Graham Walton,Department of Agriculture

6. …

Atrazine Induction Of Cytochrome P450 In Chironomus Tentans Larvae, Francisca Miota, Blair Siegfried, Michael E. Scharf, Michael J. Lydy

Department of Entomology: Faculty Publications

Cytochrome P450-dependent aldrin epoxidation was characterized in third instar larvae of the aquatic midge, Chironomus tentans. Optimal in vitro assay conditions for the epoxidase were pH 7.6 and 31°C. Activity was linear up to 40 min of incubation time and 0.5 mg microsomal protein per incubation. The activity was concentrated in the mic rosomal fraction of whole body homogenates and was NADPH-dependent. The effect of atrazine exposure on aldrin epoxidase was measured to determine if this herbicide induces cytochrome P450-dependent activity. Comparisons of control and atrazine-exposed midges indicated increased epoxidase activity as a result of atrazine exposure, and a …

Chronic Effects Of Atrazine On Tolerance Of A Benthic Diatom, Karen J. Nelson, Kyle D. Hoagland, Blair Siegfried

Department of Entomology: Faculty Publications

The effects of prior chronic exposure to atrazine on responses to subsequent acute exposures were investigated using a common benthic diatom. Clonal, axenic cultures of Craticula cuspidata were established from the Platte River (Nebraska) and obtained from a culture collection (unlikely prior exposure to atrazine). All cultures received a chronic 67-d treatment of 1 μg/L atrazine, and growth was monitored using fluorometric detection of chlorophyll a. Chronic atrazine exposure significantly reduced growth rate only during the first day of treatment (p= 0.0001); no significant effect was detected throughout the remainder of the 67-d period. Following the chronic treatment, …

Uptake And Bioconcentration Of Atrazine By Selected Freshwater Algae, Jixin Tang, Kyle D. Hoagland, Blair Siegfried

Department of Entomology: Faculty Publications

Atrazine bioconcentration and uptake were determined for eight freshwater green algae and diatoms. Atrazine uptake was extremely rapid in all species examined, with nearly 90% of total uptake occurring within the first hour of exposure. Within each division, different species had different bioconcentration capacities, although the accumulation of atrazine was consistently higher in green algae (5.43–12.73 ng/mg) than in diatoms (0.33–1.69 ng/mg). Atrazine concentrations in the algal cells were much higher than in the medium, although the total amount of atrazine taken up by algae was small relative to the total atrazine in solution (1–3%). The ability of algal cells …