Life Sciences Commons^™

Factors Associated With Acoustic Bat Presence During Spring Emergence In The Appalachian Mountains Of Western Virginia, Emily Kirk Pody

Theses, Dissertations and Capstones

Studies of threats that bats face during hibernation have increased in response to white-nose syndrome (WNS), a fungal disease that has ravaged North American bat populations. However, impacts of WNS on bat ecology during spring emergence, when bats are potentially recovering from infection and allocating resources for reproduction, is relatively understudied. As more bat species become listed at the federal and state level, the need to understand the factors associated with spring emergence is critical for improving conservation guidelines and habitat management practices. Acoustic monitoring is an efficient method for monitoring bat presence for prolonged periods of time, giving biologists …

The Comparison Of Different Wetland Fish Assemblages Over Time, Robert Edward Adelstein

Theses, Dissertations and Capstones

Wetlands provide essential ecosystem services. Historically, we have drained and filled 73% of wetlands for agricultural use throughout the United States from the 1780s to the 1980s (Dahl, 1990). A nationwide focus on restoring wetlands has since occurred. Literature on restored/mitigated wetlands is rife with examples that do and do not support the same ecosystem services as natural wetlands (Langston, 1997; Meil, 2014). Restoration of wetlands occurred at the Green Bottom Wildlife Management Area (GBWMA) over several decades. Various sections of the wetland were classified by age, water depth, and vegetation. One hypothesis was that differences in fish assemblage would …

The Inbio Barcoding Initiative Database: Dna Barcodes Of Iberian Trichoptera, Documenting Biodiversity For Freshwater Biomonitoring In A Mediterranean Hotspot, Joana Pauperio, Luis Martin Gonzalez, Jesus Martinez, Marcos A. González, Filipa Ms Martins, Joana Veríssimo, Pamela Puppo, Joana Pinto, Cátia Chaves, Catarina J. Pinho, José Manuel Grosso-Silva, Lorenzo Quaglietta, Teresa Luísa L. Silva, Pedro Sousa, Paulo Celio Alves, Nuno Fonseca, Pedro Beja, Sónia Ferreira

Biological Sciences Faculty Research

Background

The Trichoptera are an important component of freshwater ecosystems. In the Iberian Peninsula, 380 taxa of caddisflies are known, with nearly 1/3 of the total species being endemic in the region. A reference collection of morphologically identified Trichoptera specimens, representing 142 Iberian taxa, was constructed. The InBIO Barcoding Initiative (IBI) Trichoptera 01 dataset contains records of 438 sequenced specimens. The species of this dataset correspond to about 37% of Iberian Trichoptera species diversity. Specimens were collected between 1975 and 2018 and are deposited in the IBI collection at the CIBIO (Research Center in Biodiversity and Genetic Resources, Portugal) or …

Assessing The Unionid Assemblage Of The Robert C. Byrd Pool, Ohio River, Ethan Hunter Bellamy

Theses, Dissertations and Capstones

Unionid mussels are the most imperiled taxa in the United States and are vastly understudied. The entire Robert C. Byrd (RCB) pool of the Ohio River is the study area. I selected sites from the 2019 and 2013 ORSANCO RCB pool assessments that utilize random site selection across the pool. I used SCUBA to survey and collect data on unionid diversity, reproduction, and habitat. I collected 1,083 individuals over 19 species in RCB pool. I compared results from RCB pool survey to two similar surveys conducted in Greenup Pool. Both richness and abundance in the Upper Section of Greenup Pool …

Leveraging Herbaria Specimens: A New 3d Imaging Protocol And Analyses Of Diversity Of West Virginia Rosids, Zachary Scott Shamblin

Theses, Dissertations and Capstones

Herbaria are repositories of plant natural history, whose data can provide insights into the life histories of plant taxa over space and time. Recent initiatives to digitize herbarium data have made studies over broad geographic and taxonomic extents possible, with the ability of scientists to access data from large databases of herbaria. Most of the digitization initiatives have focused on flat 2D herbaria sheets, but herbaria also include 3D collections, such as fungi, mosses, lichens, and dry fruits. In Chapter 1 we propose an easy and cost-effective workflow that uses free photogrammetry software to produce 3D images of 3D herbaria …

The Future Of The Big Sagebrush Ecosystem: Plant Community Response To Grazing, Wildfire, And Invasive Annual Grass In A Changing Climate, Margaret Eleanor England

Theses, Dissertations and Capstones

Climate change is increasingly affecting the plant communities across the world. In the Western United States, these changes are affecting plant communities and their risk of invasive species, as well as the frequency and severity of wildfire. Big sagebrush (Artemisia tridentata Nutt.) ecosystems of the Western United States are facing threats of increasingly frequent wildfire and invasion of the annual grass cheatgrass (Bromus tectorum L.), which may be exacerbated by global climate change. Past studies have found cheatgrass to exhibit a positive feedback loop with wildfire, and climate change is expected to facilitate expansion of the cheatgrass …

West Virginia Herbaria: Status, Updates, And Plans, Pamela Puppo, Katharine Gregg, Donna Ford-Werntz, James Vanderhorst, Michelle Mabry, Jeremy Keene

Biological Sciences Faculty Research

The West Virginia (U.S.A.) Herbarium Curators met on December 1st, 2021, with the intent of sharing updates on the collections, fostering collaboration, learning from each other's experiences, and identifying priorities for the collections towards the future. This article presents a summary of the herbaria that were represented in this meeting.

Partnering With Archivists To Process The Manuscript Collection Present At The Marshall University Herbarium, Pamela Puppo, Lori Thompson

Biological Sciences Faculty Research

The Marshall University Herbarium (MUHW) is located on the third floor of the Science Building at Marshall University in Huntington, West Virginia, United States. It was founded in the 1930's by Dr. Frank A. Gilbert when Marshall was still Marshall College. Today, MUHW is the second largest herbarium in West Virginia with about 50,000 specimens, including 20 types. Mostly composed of vascular plants, the herbarium also contains small collections of non-vascular plants, fungi, algae, fossils, and some ethnobotanical material, mainly brought from Ecuador by one of the former curators, Dr. Dan Evans between the 1980's and 2000's. Apart from these …

Crippling Rapid Evolution Of Metastasis And Drug Resistance In A549 Non-Small Cell Lung Cancer Cells With The Clinically Relevant Hsp90 Inhibitor Auy922, Nickolas Anthony Bacon

Theses, Dissertations and Capstones

The ability for species to evolve new features in response to changing circumstances in order to survive and propagate is a ubiquitous observation on both the macroscopic and microscopic levels of living systems. It should be no surprise, then, that diseases such as cancer utilize their own forms of adaptation to perpetuate themselves when exposed to external threats. Indeed, concepts drawn from Darwinian evolution are now widely accepted to help explain certain aspects of carcinogenesis and malignant progression, the sum of which have come to be known as the theory of tumor evolution. Since metastasis and drug resistance are features …

The Effects Of Endocrine Disrupting Chemicals On Plants, Christian Mathew Pridemore

Theses, Dissertations and Capstones

Endocrine disrupting chemicals (EDCs) are a diverse group of chemicals which can have a detrimental effect on human health, affecting any physiological body system sensitive to hormonal changes. Human exposure to EDCs is inevitable due to their prevalence in modern living; EDCs are integral compounds found in everyday items with which humans interact and ingest. These, along with other indirect avenues leading to EDC exposure, pose a threat to both human health and to the health of wildlife and agricultural environments. Studies on the effects of EDCs on plants can be substantially informative and necessary for progress towards solving the …

Lynn Margulis And Boris Kozo-Polyansky: How The Symbiogenesis Was Translated From Russian, Victor Fet

Biological Sciences Faculty Research

This contribution details the complex history of the early work by Boris Kozo-Polyansky (1924) that became available in English translation 86 years after it was published in Russian. The great American naturalist Lynn Margulis—whose serial endosymbiosis theory was presciently predated by Kozo-Polyansky by four decades—was instrumental in organizing this resurrection and ‘horizontal transfer’ of knowledge, forgotten by that time even in Russia.

Revision Of The Genus Styxosaurus And Relationships Of The Late Cretaceous Elasmosaurids (Sauropterygia: Plesiosauria) Of The Western Interior Seaway, Elliott Armour Smith

Theses, Dissertations and Capstones

Growing evidence indicates that elasmosaurid plesiosaurs from the Late Cretaceous Western Interior Seaway are members of a single clade, the Styxosaurinae. The styxosaurines are reported to be mostly Campanian in age, and taxa within the clade obtain the longest necks, by number of cervical vertebrae, of any known vertebrate. The styxosaurines are morphologically diverse and include taxa that exhibit a secondary reduction in neck length. Given the evolutionary plasticity of postcranial characters in plesiosaurs in general, and neck length in elasmosaurs, scrutiny of cranial osteology is pertinent to advancing understanding of Western Interior Seaway elasmosaurids. This study finds that an …

Using Molecular Diet Analysis To Inform Invasive Species Management: A Case Study Of Introduced Rats Consuming Endemic New Zealand Frogs, Bastian Egeter, Cailín Roe, Sara Peixoto, Pamela Puppo, Luke J. Easton, Joana Pinto, Phillip J. Bishop, Bruce C. Robertson

Biological Sciences Faculty Research

The decline of amphibians has been of international concern for more than two decades, and the global spread of introduced fauna is a major factor in this decline. Conservation management decisions to implement control of introduced fauna are often based on diet studies. One of the most common metrics to report in diet studies is Frequency of Occurrence (FO), but this can be difficult to interpret, as it does not include a temporal perspective. Here, we examine the potential for FO data derived from molecular diet analysis to inform invasive species management, using invasive ship rats (Rattus rattus) …

The Genomic Landscape Of Molecular Responses To Natural Drought Stress In Panicum Hallii., John T. Lovell, Jerry Jenkins, David B. Lowry, Sujan Mamidi, Avinash Sreedasyam, Xiaoyu Weng, Kerrie Barry, Jason Bonnette, Brandon Campitelli, Chris Daum, Sean P. Gordon, Billie A. Gould, Albina Khasanova, Anna Lipzen, Alice Macqueen, Juan Diego Palacio-Mejía, Christopher Plott, Eugene V. Shakirov, Shengqiang Shu, Yuko Yoshinaga, Matt Zane, Dave Kudrna, Jason D. Talag, Daniel Rokhsar, Jane Grimwood, Jeremy Schmutz, Thomas E. Juenger

Biological Sciences Faculty Research

Environmental stress is a major driver of ecological community dynamics and agricultural productivity. This is especially true for soil water availability, because drought is the greatest abiotic inhibitor of worldwide crop yields. Here, we test the genetic basis of drought responses in the genetic model for C4 perennial grasses, Panicum hallii, through population genomics, field-scale gene-expression (eQTL) analysis, and comparison of two complete genomes. While gene expression networks are dominated by local cis-regulatory elements, we observe three genomic hotspots of unlinked trans-regulatory loci. These regulatory hubs are four times more drought responsive than the genome-wide average. Additionally, cis- and trans-regulatory …

Using Environmental Dna And Occupancy Modeling To Examine Drivers Of Eastern Hellbender Extirpation And Sampling Method Efficiency In West Virginia, Sean Michael Wineland

Theses, Dissertations and Capstones

Amphibian population declines and range constrictions are widespread but poorly understood. Effective conservation planning relies on accurate distribution data to develop a fundamental understanding of causal changes in species distributions. However, conventional detection methods for cryptic and elusive amphibians suffer from imperfect detection. Environmental DNA (eDNA) has emerged as an innovative and powerful conservation tool for detecting aquatic species presence; however comparative studies aimed at fully understanding eDNA detection probabilities are lacking. In this study, I used environmental DNA (eDNA) sampling methods and detailed historical records to identify drivers of extirpation and sampling method efficiency for an imperiled, long-lived giant …

A Characterization Of West Virginia Coyotes (Canis Latrans) Utilizing Skull Morphology, Katharina E. Scholer

Theses, Dissertations and Capstones

Coyotes (Canis latrans) are now found throughout North and Central America, but before European colonization were restricted to west of the Mississippi. Migration occurred in two major paths to the East; north over the Great Lakes (through Canada) and south below the Great Lakes. The location of these routes is significant because those migrating north interbred with the wolves that reside there. These hybrid animals are larger and behaviorally different from their western counterparts. It is possible to differentiate these hybrids morphologically and genetically. Hybrids are known to be located in Maine, New York, and Pennsylvania, but the interest of …