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Biochemistry, Biophysics, and Structural Biology

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1991

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Articles 31 - 43 of 43

Full-Text Articles in Life Sciences

Improved Syntheses Of Some Monochloro- And Monobromo-8-Quinolinols / Herman Gershon And Donald D. Clarke Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd Jan 1991

Improved Syntheses Of Some Monochloro- And Monobromo-8-Quinolinols / Herman Gershon And Donald D. Clarke Department Of Chemistry, Fordham University, Bronx, Ny 10458, Usa, Herman Gershon, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Procedures were developed for the preparation of the 2-, 3-, 4-, and 6-monosubstituted chloro and bromo 8-quinolinols which afforded greater yields and/or reduced the number of steps in the preparation. 100 MHz 1H-NMR spectra for the 12 possible monochloro and mono bromo analogues are given


Lignin Distribution In Thermomechanically Pulped Wood Fibers Via X-Ray Microanalysis, Douglas Stokke, Xuerong Wang Jan 1991

Lignin Distribution In Thermomechanically Pulped Wood Fibers Via X-Ray Microanalysis, Douglas Stokke, Xuerong Wang

Douglas D. Stokke

The three major framework organic constituents of the secondary xylem (wood) of trees are cellulose, hemicelluloses, and lignin. Lignin imparts a number of important physical properties to the woody cell wall. In addition, lignin serves as the "cement" which binds cells together, and is the material which must be softened, altered, or removed during pulping processes. The distribution of lignin within pulp cell walls is of importance with respect to use of the pulp fibers. In this study, x-ray analysis was used to investigate lignin distribution in thermomechanically pulped (i.e., pulped with heat and pressure, with no chemical treatment) aspen …


Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson Jan 1991

Monoclonal Antibodies To The Fusion Protein Of Bovine Respiratory Syncytial Virus, Kent M. Mulkey, Gary A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Five monoclonal antibodies specific for bovine respiratory syncytial virus were characterized by Western immunoblotting, radioimmunoprecipitation, and epitope mapping assays. The monoclonal antibodies were found to be specific for the fusion protein, and there were at least two antigen binding sites, one of which was neutralizing.


Fluoroacetate And Fluorocitrate: Mechanism Of Action / Donald D. Clarke, Donald Dudley Clarke Phd Jan 1991

Fluoroacetate And Fluorocitrate: Mechanism Of Action / Donald D. Clarke, Donald Dudley Clarke Phd

Chemistry Faculty Publications

The concept of lethal synthesis as suggested by Peters is reviewed in the light of the more recent work in this area. It is suggested that fluorocitrate is a "suicide" substrate for aconitase rather than a competitive inhibitor as originally suggested. The use of these substances to study glialneuronal relationships is considered


Evidence Of Steric Factors In The Fungitoxic Mechanism Of 8-Quinolinol And Its 5- And 7-Halogenated Analogues / Herman Gershon, Donald D. Clarke, And Muriel Gershon, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon Jan 1991

Evidence Of Steric Factors In The Fungitoxic Mechanism Of 8-Quinolinol And Its 5- And 7-Halogenated Analogues / Herman Gershon, Donald D. Clarke, And Muriel Gershon, Herman Gershon, Donald Dudley Clarke Phd, Muriel Gershon

Chemistry Faculty Publications

Antifungal studies were made of mixtures of minimal inhibitory concentrations (MICs) of 8-quinolinol and its 5- and 7-halo analogues against six fungi: Aspergillus niger, A. oryzae, Trichoderma viride, Myrothecium verrucaria, Mucor cirinelloides, and Trichophyton mentagrophytes. Mixtures of 8-quinolinol with 5- or 7-fluoro-8-quinolinol and of 5- and 7-fluoro-8-quinolinol showed additive activity, and their respective toxicities were reversed by L-cysteine. These results suggested a common mechanism of activity for the three toxicants. Potentiation of the fungitoxicity of mixtures of 8-quinolinol and its 5- and 7-chloro, bromo, and iodo analogues, as well as mixtures of 5- and 7-chloro, 5- and 7-bromo, and 5- …


Immunological Identification Of A Centrin Homologue In The Red Alga Gracilaria Tikvahiae, Christopher Lee Dassler Jan 1991

Immunological Identification Of A Centrin Homologue In The Red Alga Gracilaria Tikvahiae, Christopher Lee Dassler

Dissertations, Theses, and Masters Projects

No abstract provided.


Monooxygenase Induction And Lethality As Endpoints In Aquatic Toxicology, Daniel W. Sved Jan 1991

Monooxygenase Induction And Lethality As Endpoints In Aquatic Toxicology, Daniel W. Sved

Dissertations, Theses, and Masters Projects

Spot, Leiostomus xanthurus, were exposed to suspended sediments (&\approx&20 mg/L) contaminated with polycyclic aromatic hydrocarbons (PAH) in a laboratory flow-through system to evaluate the applicability of hepatic ethoxyresorufin O-deethylase (EROD) induction as an indicator of PAH exposure. PAH sources tested were coal-tar creosote (CTC), a low molecular weight fraction of creosote (LMWF), and a high molecular weight fraction of creosote (HMWF). A standard 96-h acute toxicity test was conducted to ensure that PAH concentrations tested in induction studies were sub-acutely toxic. The 96-h LC50 for spot was 1740 &\mu&g PAH/L (95% confidence interval = 1480-2060 &\mu&g PAH/L). The lowest concentration …


Nicotinate, Nicotinamide, And The Reactivity Of Leghemoglobin In Soybean Root Nodules, Robert V. Klucas, Cyril A. Appleby Jan 1991

Nicotinate, Nicotinamide, And The Reactivity Of Leghemoglobin In Soybean Root Nodules, Robert V. Klucas, Cyril A. Appleby

Department of Biochemistry: Faculty Publications

Nicotinate has been postulated to interfere with the binding of O2 to ferrous leghemoglobin in soybean (Glycine max) root nodules. For such a function, the levels of nicotinate in nodules must be sufficiently high to bind a significant amount of leghemoglobin. We have measured levels of nicotinate, nicotinamide, and leghemoglobin in soybean nodules from plants 34 to 73 days after planting in a glasshouse. On a per gram nodule fresh weight basis, levels between 10.4 and 21 nanomoles for nicotinate, 19.2 and 37.8 nanomoles for nicotinamide, and 170 to 280 nanomoles for leghemoglobin were measured. Even if …


Detection Of Mercuric Ions In Water By Elisa With A Mercury- Specific Antibody, Dwane E. Wylie, Larry D. Carlson, Randy Carlson, Fred W. Wagner, Sheldon M. Schuster Jan 1991

Detection Of Mercuric Ions In Water By Elisa With A Mercury- Specific Antibody, Dwane E. Wylie, Larry D. Carlson, Randy Carlson, Fred W. Wagner, Sheldon M. Schuster

Department of Biochemistry: Faculty Publications

An immunoassay that detects mercuric ions in water at concentrations of 0.5 ppb and above is described. The assay utilizes a monoclonal antibody that binds specifically to mercuric ions immobilized in wells of microtiter plates. Within the range of 0.5-10 ppb mercury, the absorbance in the enzyme-linked immunosorbent assay (ELISA) is linear to the log of the mercuric ion concentration. The quantitation of mercury by ELISA correlates closely with results from cold-vapor atomic absorption. Other divalent metal cations do not interfere with the assay, although there is interference in the presence of 1 mM chloride ions. The optimum pH for …


Photosynthetic Electron Transport In Genetically Altered Photosystem Ii Reaction Centers Of Chloroplasts, Robin A. Roffey, John H. Golebeck, C. Russ Hille, Richard T. Sayre Jan 1991

Photosynthetic Electron Transport In Genetically Altered Photosystem Ii Reaction Centers Of Chloroplasts, Robin A. Roffey, John H. Golebeck, C. Russ Hille, Richard T. Sayre

Department of Biochemistry: Faculty Publications

Using a cotransformation system to identify chloroplast transformants in Chlamydomonas reinhardtii, we converted histidine-195 of the photosystem H reaction center D1 protein to a tyrosine residue. The mutants were characterized by a reduced quantum efficiency for photosynthetic oxygen evolution, which varied in a pH-dependent manner, a reduced capacity to oxidize artificial donors to photosystem II, and P680+) reduction kinetics (microsecond) that were essentially similar to wild type. In addition, a dark-stable radical was detected by ESR in mutant photosystem II particles but not in wild-type particles. This radical was similar in g value and lineshape to chlorophyll …


Evaluation Of A Technique To Quantify Lipids In Bivalve Larvae Crassostrea Virginica Gmelin, Using Nile Red And Epiflourescence Microscopy, Laura Liliana Castell Perez Jan 1991

Evaluation Of A Technique To Quantify Lipids In Bivalve Larvae Crassostrea Virginica Gmelin, Using Nile Red And Epiflourescence Microscopy, Laura Liliana Castell Perez

Dissertations, Theses, and Masters Projects

No abstract provided.


Cell Surface-Binding Sites For Progesterone Mediate Calcium Uptake In Human Sperm, Peter F. Blackmore, Joseph Neulan, Frank Lattanzio, Stephen J. Beebe Jan 1991

Cell Surface-Binding Sites For Progesterone Mediate Calcium Uptake In Human Sperm, Peter F. Blackmore, Joseph Neulan, Frank Lattanzio, Stephen J. Beebe

Bioelectrics Publications

Recent studies (e.g. Blackmore, P. F., Beebe, S. J., Danforth, D. R., and Alexander, N.) (1990) J. Biol. Chem. 265, 1376-1380) have shown that in human sperm, progesterone produces a rapid increase in intracellular free calcium ([Ca2+]i) and an induction of the acrosome reaction (e.g. Osman, R. A., Andria, M. L., Jones, A. D., and Meizel, S. (1989) Biochem. Biophys. Res. Commun. 160, 828-833). In this study, the location of progesterone receptors on the cell surface of human sperm was identified using progesterone immobilized on bovine serum albumin (BSA) (progesterone 3-(O-carboxymethyl)oxime:BSA) as well as progesterone and its 3-O-carboxymethyloxime derivative. Using …


Phenology And Bionomics Of Culicoides (Diptera: Ceratopogonidae) In Glynn County, Georgia, Lisa M. Westberry Jan 1991

Phenology And Bionomics Of Culicoides (Diptera: Ceratopogonidae) In Glynn County, Georgia, Lisa M. Westberry

Legacy ETDs

Seasonal abundance and population densities of Culicoides species from sites in Glynn County, Georgia were surveyed using 15 New Jersey light traps during a 9-month period from April-December 1990. Environmental effects on the abundances were also analyzed. The three primary species present were: Culicoides furens (Poey), C. hollensis Mellander & Brues, and C. melleus (Coguillett). C. furens were collected year round, but predominantly during the summer. C. hollensis had a bimodal abundance (spring and fall) . C. melleus also had a bimodal abundance (late summer and fall).

Larval habitats of the primary species were identified …