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A Biochemical Study Of Gossypol And Lactate Dehydrogenase X Binary Interactions, Patricia Brown Ravenell
A Biochemical Study Of Gossypol And Lactate Dehydrogenase X Binary Interactions, Patricia Brown Ravenell
Theses and Dissertations in Biomedical Sciences
The goal of this project was to study the mechanism of action of gossypol through its (a) binary interaction with native and trypsin digested lactate dehydrogenase-X (LD-X), a sperm-specific isozyme, and (b) its binding in vitro in primary cultures of spermatogenic cells. Mouse LD-X was cleaved with trypsin before and after treatment with gossypol. This was followed by high performance chromatography (HPLC) separation of the LD-X tryptic peptide fragments to observed alterations in separation patterns as indicators of intramolecular disturbance in the enzyme molecule. Definite alterations in peptide fragment peaks were observed in the presence of gossypol and suggest conformational …