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Articles 31 - 60 of 112
Full-Text Articles in Life Sciences
Anti-Mullerian Hormone: Above And Beyond Conventional Ovarian Reserve Markers, Zehra Jamil, Syeda Sadia Fatima, Khalid Ahmed, Rabia Malik
Anti-Mullerian Hormone: Above And Beyond Conventional Ovarian Reserve Markers, Zehra Jamil, Syeda Sadia Fatima, Khalid Ahmed, Rabia Malik
Department of Biological & Biomedical Sciences
Management of ovarian dysfunctions requires accurate estimation of ovarian reserve (OR). Therefore, reproductive hormones and antral follicle count (AFC) are assessed to indicate OR. Serum anti-Mullerian hormone (AMH) is a unique biomarker that has a critical role in folliculogenesis as well as steroidogenesis within ovaries. Secretion from preantral and early antral follicles renders AMH as the earliest marker to show OR decline. In this review we discuss the dynamics of circulating AMH that remarkably vary with sex and age. As it emerges as a marker of gonadal development and reproductive disorders, here we summarize the role of AMH in female …
Students' Perception Of Educational Environment At Aga Khan University Medical College, Karachi, Pakistan, Rehana Rehman, Kulsoom Ghias, Syeda Sadia Fatima, Mehwish Hussain, Faiza Alam
Students' Perception Of Educational Environment At Aga Khan University Medical College, Karachi, Pakistan, Rehana Rehman, Kulsoom Ghias, Syeda Sadia Fatima, Mehwish Hussain, Faiza Alam
Department of Biological & Biomedical Sciences
Objective: To assess educational environment in Aga Khan University Medical College (AKUMC) by Dundee Ready Educational Environmental Measure (DREEM) scale.
Methods: A cross-sectional survey of students at the AKUMC with simple random sampling was carried out from June 2014 till March 2015. Responses in five subscales were used to calculate DREEM scores.
Results: The average DREEM score was 125.77±16.8 with a reliability of 91.3%. With regards to subscales, on the 12-item students' perceptions of learning (PoL) subscale, the maximum score was 48; 11 items of students' perceptions of teachers (PoT) had a maximum score of 44; students' academic self-perceptions (ASP) …
Use Of Follicular Output Rate To Predict Intracytoplasmic Sperm Injection Outcome, Rehana Rehman, Rozina Mustafa, Mukhtiar Baig, Sara Arif, Muhammad Faisal Hashmi
Use Of Follicular Output Rate To Predict Intracytoplasmic Sperm Injection Outcome, Rehana Rehman, Rozina Mustafa, Mukhtiar Baig, Sara Arif, Muhammad Faisal Hashmi
Department of Biological & Biomedical Sciences
Background: The measurement of follicular output rate (FORT) has been proposed as a good indicator for evaluating follicular response to the exogenous recombinant folliclestimulating hormone (rFSH). This places FORT as a promising qualitative marker for ovarian function. The objective of the study was to determine FORT as a predictor of oocyte competence, embryo quality and clinical pregnancy after intracytoplasmic sperm injection (ICSI).
Materials and Methods: This prospective study was carried out on a group of infer- tile females (n=282) at Islamabad Clinic Serving Infertile Couples, Islamabad, Pakistan, from June 2010 till August 2013. Downregulated females were stimulated in injection gonadotropins …
Kcnq1 Rs2237895 Polymorphism Is Associated With Gestational Diabetes In Pakistani Women, Syeda Sadia Fatima, Bushra Chaudhry, Taseer Ahmed Khan, Saad Farooq
Kcnq1 Rs2237895 Polymorphism Is Associated With Gestational Diabetes In Pakistani Women, Syeda Sadia Fatima, Bushra Chaudhry, Taseer Ahmed Khan, Saad Farooq
Department of Biological & Biomedical Sciences
Background and Objective: Genetic studies on gestational diabetes (GDM) are relatively scarce; moreover, limited data is available for KCNQ1 polymorphism in Pakistani pregnant women. We aimed to determine the frequency of KCNQ1 rs2237895 in GDM and normal pregnant controls and its association with GDM-related phenotypes.
Methods: A total of 637 pregnant females (429 controls and 208 cases) in their second trimester were classified according to the International Association of the Diabetes and Pregnancy Study criteria in this study. Their blood samples were genotyped for KCNQ1 SNP rs2237895 using PCR-RFLP method and sequencing. Fasting and two hour-post glucose load blood levels, …
Fsh/Lh Ratio In Females And Intracytoplasmic Sperm Injection, Rehana Rehman, Hareem Syed, Najeeha Talat Iqbal, Sara Arif, Saeeda Sheharyar
Fsh/Lh Ratio In Females And Intracytoplasmic Sperm Injection, Rehana Rehman, Hareem Syed, Najeeha Talat Iqbal, Sara Arif, Saeeda Sheharyar
Department of Biological & Biomedical Sciences
Objective: To observe the effect of follicle stimulating hormone-luteinizing hormone ratio on oocyte parameters, quality of embryo, implantation and clinical pregnancy rate after intracytoplasmic sperm injection.
Methods: The retrospective study was conducted at a fertility clinic in Islamabad, Pakistan, and comprised data of primary infertile females who underwent intracytoplasmic sperm injection from June 2011 to March 2013. All subjects had duration of infertility more than two years, and age range was 20-40 years. Follicle stimulating hormone and luteinizing hormone were estimated by enzyme-linked immunosorbent assay on day 3 of the cycle and the ratio was calculated. Groups were stratified on …
Effect Of Raised Body Fat On Vitamin D, Leptin And Bone Mass, Syeda Sadia Fatima, Saad Farooq, Maaida Asif Tauni, Omar Irfan, Faiza Alam
Effect Of Raised Body Fat On Vitamin D, Leptin And Bone Mass, Syeda Sadia Fatima, Saad Farooq, Maaida Asif Tauni, Omar Irfan, Faiza Alam
Department of Biological & Biomedical Sciences
Objective: To estimate leptin, vitamin D and bone mineral density levels in individuals with high fat mass, and to assess any correlation.Methods: The cross-sectional study was conducted at the Basic Medical Sciences Institute, Jinnah Post Graduate Medical Centre, Karachi, and Aga Khan University, Karachi, from August 2012 to July 2014, and comprised healthy male volunteers between the ages of 18-60 years. Body fat percentage was determined using bioelectrical impedance analysis and the participants were classified as: Group A (15-21.9); Group B (22-27.9); and Group C ( > 28). Bone mineral density was calculated by ultrasound bone densitometer (T-score between +1 and …
Short Communication: Lack Of Association Between Mthfr Gene Polymorphisms And Response To Methotrexate Treatment In Pakistani Patients With Rheumatoid Arthritis, Mohammad Perwaiz Iqbal, Azra Arif Ali, Naseema Mehboobali, Khalida Iqbal
Short Communication: Lack Of Association Between Mthfr Gene Polymorphisms And Response To Methotrexate Treatment In Pakistani Patients With Rheumatoid Arthritis, Mohammad Perwaiz Iqbal, Azra Arif Ali, Naseema Mehboobali, Khalida Iqbal
Department of Biological & Biomedical Sciences
Methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms have been reported to be associated with response to methotrexate (MTX) in certain populations of patients with rheumatoid arthritis (RA). This study aims at investigating any relationship of two single nucleotide polymorphisms (SNPs) in MTHFR gene, C677T and A1298C with response to therapy with MTX in Pakistani RA patients. Allelic frequencies of the two polymorphisms (C677T and A1298C) were determined in 67 RA patients (9 males and 58 females; mean age 42.87 ± 13.5 years) who had previously participated in a prospective clinical trial. Fifty-one patients had received MTX and were followed up for response …
Producing Financially Literate Medical Graduates -- A National Need For A Brighter Pakistan, Rehana Rehman, Shah Jahan Katpar, Mehwish Hussain, Rakhshaan Khan
Producing Financially Literate Medical Graduates -- A National Need For A Brighter Pakistan, Rehana Rehman, Shah Jahan Katpar, Mehwish Hussain, Rakhshaan Khan
Department of Biological & Biomedical Sciences
Objective: To compare the awareness of financial wellness between male and female medical students.Methods: The cross-sectional questionnaire-based survey was conducted from February to December 2011 and comprised first year medical students belonging to different medical colleges of Karachi. Students from both genders, aged 19-24 years, belonging to all ethnic groups willing to participate were included. A close-ended questionnaire evaluated aspects of financial wellness, using four-point Likert's scale: never, sometimes, mostly, and always from 0 to 4. Besides, factor analysis was performed to investigate common financial wellness among the participants.Results: Of the total 800 students approached, complete responses were received from …
Thyroxine Replacement In Patients On Parenteral Nutrition: A Clinical Conundrum, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Thyroxine Replacement In Patients On Parenteral Nutrition: A Clinical Conundrum, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Department of Biological & Biomedical Sciences
No abstract provided.
The Artificial Divide Between Biomedical Sciences And Clinical Medicine In Undergraduate Medical Education, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
The Artificial Divide Between Biomedical Sciences And Clinical Medicine In Undergraduate Medical Education, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Department of Biological & Biomedical Sciences
No abstract provided.
Cellulose Degradation: A Therapeutic Strategy In The Improved Treatment Of Acanthamoeba Infections, Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Cellulose Degradation: A Therapeutic Strategy In The Improved Treatment Of Acanthamoeba Infections, Sahreena Lakhundi, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
Acanthamoeba is an opportunistic free-living amoeba that can cause blinding keratitis and fatal brain infection. Early diagnosis, followed by aggressive treatment is a pre-requisite in the successful treatment but even then the prognosis remains poor. A major drawback during the course of treatment is the ability of the amoeba to enclose itself within a shell (a process known as encystment), making it resistant to chemotherapeutic agents. As the cyst wall is partly made of cellulose, thus cellulose degradation offers a potential therapeutic strategy in the effective targeting of trophozoite encased within the cyst walls. Here, we present a comprehensive report …
How Pakistan's Media Spreads The Message About Reproductive And Sexual Health, Syed H. Abidi, Muhammad Raees, Syed Ali
How Pakistan's Media Spreads The Message About Reproductive And Sexual Health, Syed H. Abidi, Muhammad Raees, Syed Ali
Department of Biological & Biomedical Sciences
Abstract, issue, and pagination are not provided by the author/publisher
Severe Ovarian Hyperstimulation Syndrome Complicated By Stenotrophomonas Maltophilia Peritonitis: A Case Report And Literature Review, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Severe Ovarian Hyperstimulation Syndrome Complicated By Stenotrophomonas Maltophilia Peritonitis: A Case Report And Literature Review, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Department of Biological & Biomedical Sciences
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic disorder resulting from ovulation induction. Although the occurrence of this disorder is rare, it can be potentially life-threatening in its most severe forms. We herein present the case of a young nulliparous woman who presented with features of abdominal compartment syndrome and was subsequently diagnosed with severe OHSS. All physicians, in particular critical care doctors, must be aware of this rare, but potentially life-threatening iatrogenic disorder.
Stress Management In Cyst-Forming Free-Living Protists: Programmed Cell Death And/Or Encystment, Naveed Ahmed Khan, Junaid Iqbal, Ruqaiyyah Siddiqui
Stress Management In Cyst-Forming Free-Living Protists: Programmed Cell Death And/Or Encystment, Naveed Ahmed Khan, Junaid Iqbal, Ruqaiyyah Siddiqui
Department of Biological & Biomedical Sciences
In the face of harsh conditions and given a choice, a cell may (i) undergo programmed cell death, (ii) transform into a cancer cell, or (iii) enclose itself into a cyst form. In metazoans, the available evidence suggests that cellular machinery exists only to execute or avoid programmed cell death, while the ability to form a cyst was either lost or never developed. For cyst-forming free-living protists, here we pose the question whether the ability to encyst was gained at the expense of the programmed cell death or both functions coexist to counter unfavorable environmental conditions with mutually exclusive phenotypes.
Prevalence Of Obesity And Overweight, Its Clinical Markers And Associated Factors In A High Risk South-Asian Population, Faridah Amin, Syeda Sadia Fatima, Najmul Islam, Anwar H. Gilani
Prevalence Of Obesity And Overweight, Its Clinical Markers And Associated Factors In A High Risk South-Asian Population, Faridah Amin, Syeda Sadia Fatima, Najmul Islam, Anwar H. Gilani
Department of Biological & Biomedical Sciences
Background: Obesity is a global epidemic, which is a risk factor for cardiovascular diseases and metabolic abnormalities. It is measured by body mass index (BMI), waist circumference (WC), waist-hip ratio (WHR), body fat (BF) distribution and abdominal fat mass, each having its own merits and limitations. Variability in body composition between ethnic groups in South-Asians is significant and may not be truly reflected by BMI alone, which may result in misclassification. This study therefore, aims to determine the frequency of obesity, body fat composition and distribution, in a high risk population of an urban slum of Karachi, Pakistan. This survey …
Increased Body Mass Index May Lead To Hyperferritinemia Irrespective Of Body Iron Stores, Faiza Alam, Abdul Shakoor Memon, Syeda Sadia Fatima
Increased Body Mass Index May Lead To Hyperferritinemia Irrespective Of Body Iron Stores, Faiza Alam, Abdul Shakoor Memon, Syeda Sadia Fatima
Department of Biological & Biomedical Sciences
Objective: Obesity causes subclinical inflammation which results in the secretion of various bioactive peptides that are key players in metabolic regulation of iron homeostasis. We sought to establish correlation of one such peptide (ferritin) with marker of subclinical inflammation (CRP) in various BMI.
Methods: Total 150 subjects between the ages of 20-60 years were included in the cross-sectional study conducted at Basic Medical Sciences Institute, Jinnah Post Graduate Medical Centre, Karachi, Pakistan. Body Mass Index (BMI) was calculated by weight (kg) /height (m(2)). The given values were used as reference for Group A: normal weight (18.0-22.9 kg/m2), Group B: overweight …
Gossypiboma: An Unrecognized And Under-Reported Problem In Pakistan, Abdul Rehman, Noor-Ul-Ain Baloch, Muhammad Awais
Gossypiboma: An Unrecognized And Under-Reported Problem In Pakistan, Abdul Rehman, Noor-Ul-Ain Baloch, Muhammad Awais
Department of Biological & Biomedical Sciences
No abstract provided.
Primary Amoebic Meningoencephalitis Caused By Naegleria Fowleri: An Old Enemy Presenting New Challenges, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Primary Amoebic Meningoencephalitis Caused By Naegleria Fowleri: An Old Enemy Presenting New Challenges, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
First discovered in 1899, Naegleria fowleri is a protist pathogen, known to infect the central nervous system and produce primary amoebic meningoencephalitis. The most distressing aspect is that the fatality rate has remained more than 95%, despite our advances in antimicrobial chemotherapy and supportive care. Although rare worldwide, most cases have been reported in the United States, Australia, and Europe (France). A large number of cases in developing countries go unnoticed. In particular, religious, recreational, and cultural practices such as ritual ablution and/or purifications, Ayurveda, and the use of neti pots for nasal irrigation can contribute to this devastating infection. …
Low Doses Of Colony-Stimulating Factors Lead To Resolution Of Neutropenia In Cancer Patients Through Increased Levels Of Dihydrofolate Reductase, Mohammad Perwaiz Iqbal, Ikram Ali Burney
Low Doses Of Colony-Stimulating Factors Lead To Resolution Of Neutropenia In Cancer Patients Through Increased Levels Of Dihydrofolate Reductase, Mohammad Perwaiz Iqbal, Ikram Ali Burney
Department of Biological & Biomedical Sciences
Low doses of granulocyte- colony stimulating factor (G-CSF) and granulocyte macrophage- colony stimulating factor (GM-CSF) have been shown to be beneficial in reducing duration of systemic antibiotic therapy and in-patient hospitalization by decreasing the period of neutropenia in cancer patients undergoing chemotherapy. Since the underlying mechanism is unclear, the aim of this study was to investigate whether the administration of G-CSF and GM-CSF in two different doses (low dose and standard dose) would result into resolution of neutropenia with concomitant increase in multiple forms of dihydrofolate reductase (DHFR, a pivotal enzyme in the pathway of de novo DNA synthesis). Thirty …
Cholesterol-Cholate-Butterfat Diet Offers Multi-Organ Dysfunction In Rats, Humaira Jamshed, Jamshed Arslan, Anwar-Ul-Hassan Gilani
Cholesterol-Cholate-Butterfat Diet Offers Multi-Organ Dysfunction In Rats, Humaira Jamshed, Jamshed Arslan, Anwar-Ul-Hassan Gilani
Department of Biological & Biomedical Sciences
Background: Comparable to commercial expensive high-fat diets, cholesterol-cholate-butterfat (CCB) diet has also been used to induce hyperlipidemia in rats. Our objective was to explore its influence on multiple organs. Consequence of fasting was also analysed.
Methods: Rats in groups 1 and 2 received normal diet (ND) whereas groups 3 and 4 received CCB-diet. Food was withdrawn daily for two hours from groups 2 (ND-F) and 4 (CCB-F). Blood was collected at fourth and sixth week for biochemical estimation; Morris water maze was done in the sixth week for learning ability and memory; after which aortae were isolated for vascular reactivity. …
Gossypiboma Diagnosed Fifteen Years After A Cesarean Section: A Case Report, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Gossypiboma Diagnosed Fifteen Years After A Cesarean Section: A Case Report, Abdul Rehman, Noor Ul-Ain Baloch, Muhammad Awais
Department of Biological & Biomedical Sciences
Gossypiboma, a retained surgical sponge, is a rare complication following any surgical procedure and is primarily a result of human error. Such patients often have vague clinical presentations and the diagnosis often comes as a surprise. We present the case of a 40-year-old woman who had a long-standing history of lower abdominal discomfort. She was diagnosed with a gossypiboma fifteen years after a cesarean section. All general physicians, surgeons and radiologists must be aware of this rare but clinically significant entity.
Interactions Of Neuropathogenic Escherichia Coli K1 (Rs218) And Its Derivatives Lacking Genomic Islands With Phagocytic Acanthamoeba Castellanii And Nonphagocytic Brain Endothelial Cells, Farzana Abubakar Yousuf, Zuhair Yousuf, Junaid Iqbal, Ruqaiyyah Siddiqui, Hafsa Khan, Naveed Ahmed Khan
Interactions Of Neuropathogenic Escherichia Coli K1 (Rs218) And Its Derivatives Lacking Genomic Islands With Phagocytic Acanthamoeba Castellanii And Nonphagocytic Brain Endothelial Cells, Farzana Abubakar Yousuf, Zuhair Yousuf, Junaid Iqbal, Ruqaiyyah Siddiqui, Hafsa Khan, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
Here we determined the role of various genomic islands in E. coli K1 interactions with phagocytic A. castellanii and nonphagocytic brain microvascular endothelial cells. The findings revealed that the genomic islands deletion mutants of RS218 related to toxins (peptide toxin, alpha -hemolysin), adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), protein secretion system (T1SS for hemolysin), invasins (IbeA, CNF1), metabolism (D-serine catabolism, dihydroxyacetone, glycerol, and glyoxylate metabolism) showed reduced interactions with both A. castellanii and brain microvascular endothelial cells. Interestingly, the deletion of RS218-derived genomic island 21 containing adhesins (P fimbriae, F17-like fimbriae, nonfimbrial adhesins, Hek, and hemagglutinin), …
Self-Reported Attitudes And Behaviours Of Medical Students In Pakistan Regarding Academic Misconduct: A Cross-Sectional Study, Kulsoom Ghias, Ghulam Rehmani Lakho, Hamna Asim, Iqbal Syed Azam, Sheikh Abdul Saeed
Self-Reported Attitudes And Behaviours Of Medical Students In Pakistan Regarding Academic Misconduct: A Cross-Sectional Study, Kulsoom Ghias, Ghulam Rehmani Lakho, Hamna Asim, Iqbal Syed Azam, Sheikh Abdul Saeed
Department of Biological & Biomedical Sciences
Background: Honesty and integrity are key attributes of an ethically competent physician. However, academic misconduct, which includes but is not limited to plagiarism, cheating, and falsifying documentation, is common in medical colleges across the world. The purpose of this study is to describe differences in the self-reported attitudes and behaviours of medical students regarding academic misconduct depending on gender, year of study and type of medical institution in Pakistan.
Methods: A cross sectional study was conducted with medical students from one private and one public sector medical college. A pre-coded questionnaire about attitudes and behaviours regarding plagiarism, lying, cheating and …
Culturable Aerobic And Facultative Anaerobic Intestinal Bacterial Flora Of Black Cobra (Naja Naja Karachiensis) In Southern Pakistan, Junaid Iqbal, Mehwish Sagheer, Nazneen Tabassum, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Culturable Aerobic And Facultative Anaerobic Intestinal Bacterial Flora Of Black Cobra (Naja Naja Karachiensis) In Southern Pakistan, Junaid Iqbal, Mehwish Sagheer, Nazneen Tabassum, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
Using morphological analysis and biochemical testing, here for the first time, we determined the culturable gut bacterial flora (aerobes and facultative anaerobes) in the venomous Black Cobra (Naja naja karachiensis) from South Asia. The findings revealed that these snakes inhabit potentially pathogenic bacteria including Serratia marcescens, Pseudomonas aeruginosa, Shewanella putrefaciens, Aeromonas hydrophila, Salmonella sp., Moraxella sp., Bacillus sp., Ochrobactrum anthropi, and Providencia rettgeri. These findings are of concern, as injury from snake bite can result in wound infections and tissue necrosis leading to sepsis/necrotizing fasciitis and/or expose consumers of snake meat/medicine in the community to infections.
Treating Cancer With Heat: Hyperthermia As Promising Strategy To Enhance Apoptosis, Kanwal Ahmed, Syed Faisal Zaidi
Treating Cancer With Heat: Hyperthermia As Promising Strategy To Enhance Apoptosis, Kanwal Ahmed, Syed Faisal Zaidi
Department of Biological & Biomedical Sciences
The fundamental idea and the effects of heat on cancer cells are well known. However, the results obtained in therapy by hyperthermia (HT) alone have been only partially satisfactory. Treatment at temperatures between .40 and 44 degrees C is cytotoxic for cells in an environment with a low oxygen partial pressure and low pH, conditions that are found specifically within tumour tissue, due to insufficient blood perfusion. Under such conditions radiotherapy is less effective, and systemically applied cytotoxic agents will reach such areas in lower concentrations than in well-perfused areas. Therefore, clinically, it is preferred to use hyperthermia in combination …
Serene Threonine Kinase 39 Gene Single Nucleotide A-G Polymorphism Rs35929607 Is Weakly Associated With Essential Hypertension In Population Of Tharparkar, Pakistan, Loung Vasandas Umedani, Bushra Chaudhry, Vikram Mehraj, Muhammad Ishaq
Serene Threonine Kinase 39 Gene Single Nucleotide A-G Polymorphism Rs35929607 Is Weakly Associated With Essential Hypertension In Population Of Tharparkar, Pakistan, Loung Vasandas Umedani, Bushra Chaudhry, Vikram Mehraj, Muhammad Ishaq
Department of Biological & Biomedical Sciences
Objective: To study the prevalence of Serine Threonine Kinase-39 A-G polymorphism rs3592960 in rural population.
Methods: The random, cross-sectional study was carried out from 2009 to 2010 on 528 subjects in Tharparkar, Pakistan. Data was recorded excluding cases of secondary hypertension. Normotensives were used as the controls and hypertensives as the cases. Genotyping was carried out by tetra-primer amplification refraction mutation system-polymerase chain reaction method and the data was analysed statistically with SPSS-14.
Results: The association of Serine Threonine Kinase-39 rs35929607 with essential hypertension was as 3.07 (95% confidence interval 2.10-4.49) units/mmHg per G allele (p = 0.001). Raised systolic …
Acanthamoeba Castellanii Of The T4 Genotype Is A Potential Environmental Host For Enterobacter Aerogenes And Aeromonas Hydrophila, Farzana Abubakar Yousuf, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Acanthamoeba Castellanii Of The T4 Genotype Is A Potential Environmental Host For Enterobacter Aerogenes And Aeromonas Hydrophila, Farzana Abubakar Yousuf, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
Background: Acanthamoeba can interact with a wide range of microorganisms such as viruses, algae, yeasts, protists and bacteria including Legionella pneumophila, Pseudomonas aeruginosa, Vibrio cholerae, Helicobacter pylori, Listeria monocytogenes, Mycobacterium spp., and Escherichia coli. In this capacity, Acanthamoeba has been suggested as a vector in the transmission of bacterial pathogens to the susceptible hosts.
Methods: Here, we used a keratitis isolate of A. castellanii of the T4 genotype and studied its interactions with two bacterial genera which have not been tested before, Enterobacter aerogenes, and Aeromonas hydrophila, as well as E. coli. Assays were performed to determine bacterial association with …
Epidemiological Ins And Outs Of Helicobacter Pylori: A Review, Jibran Sualeh Muhammad, Syed Faisal Zaidi, Toshiro Sugiyama
Epidemiological Ins And Outs Of Helicobacter Pylori: A Review, Jibran Sualeh Muhammad, Syed Faisal Zaidi, Toshiro Sugiyama
Department of Biological & Biomedical Sciences
Helicobacter pylori infection is now recognised as a worldwide problem. It is the most common cause of chronic gastritis, and is strongly linked to peptic ulcer disease and gastric cancer. A comprehensive understanding of epidemiological ins and outs of H. pylori infection is very important in solving the patho-physiological enigma and might help in leading towards better management and prognosis of infection. This article presents a review of the literature on the epidemiology of H. pylori infection from 2006 to 2011. The authors used Medical Subject Heading (MeSH) terms 'Helicobacter' with 'epidemiology,' 'transmission' or 'risk factor' to search PubMed database. …
Effects Of Betel Nut On Cardiovascular Risk Factors In A Rat Model, Mohammad Perwaiz Iqbal, Naseema Mehboobali, Ghulam Haider, Shahid Pervez, Iqbal Azam
Effects Of Betel Nut On Cardiovascular Risk Factors In A Rat Model, Mohammad Perwaiz Iqbal, Naseema Mehboobali, Ghulam Haider, Shahid Pervez, Iqbal Azam
Department of Biological & Biomedical Sciences
Background: Areca nut (commonly known as betel nut) chewing has been shown to be associated with metabolic syndrome and cardiovascular disease (CVD). The mechanism by which betel nut ingestion could lead to development of CVD is not precisely known; however, dyslipidemia, hyperhomocysteinemia, hypertriglyceridemia and inflammation could be some of the potential risk factors. This study was undertaken to investigate the effects of two dosages of betel nut on homocysteinemia, inflammation and some of the components of metabolic syndrome, such as hypertriglyceridemia, low HDL-cholesterol, obesity and fasting hyperglycemia in a rat model.
Methods: Thirty-six adult female Sprague Dawley rats, aged 10–12 …
Photochemotherapeutic Strategies Against Acanthamoeba Keratitis, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Photochemotherapeutic Strategies Against Acanthamoeba Keratitis, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Department of Biological & Biomedical Sciences
Here, we determined the potential of photochemotherapy, namely the application of photodynamic compounds followed by exposure to a suitable source of UV-visible radiation against corneal pathogen, Acanthamoeba. Organometallic macromolecule, tin porphyrin [Sn(IV)porphyrin] was synthesized and purity confirmed using nuclear magnetic resonance spectroscopy. The Sn(IV)porphyrin was tested against a keratitis isolate of Acanthamoeba castellanii belonging to the T4 genotype using growth and viability assays. The effects of Sn(IV)porphyrin on A. castellanii binding to and cytopathogenicity of human corneal epithelial cells in vitro were tested. The metalloporphyrin showed potent amoebistatic effects. The tin porphyrin inhibited amoebae binding to and cytopathogenicity of corneal …