Mechanical Engineering Commons^™

Study Of Cell Viability During Laser Direct Writing Of Cell-Alginate Suspension, Hemanth Gudapati

All Theses

Laser-assisted cell printing, developed based on Matrix-assisted pulsed-laser evaporation direct-write (MAPLE DW), a typical LIFT (laser-induced forward transfer) practice, has been emerging as one of the most promising biofabrication techniques. Alginate, particularly sodium alginate, is extensively used as the constituent of bioink in laser-assisted cell printing. However, thus far, studies investigating the effect of alginate gelation on cell viability in laser-assisted cell printing are lacking. The objective of this study is to investigate the effects of gelation, gelation time, sodium alginate concentration, and the effect of operating conditions such as the laser fluence on post-transfer cell viability during laser-assisted cell …