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Full-Text Articles in Mechanical Engineering
Articular Cartilage Mechanical And Biochemical Property Relations Before And After In Vitro Growth, Timothy Ficklin, Gregory Thomas, James C. Barthel, Anna Asanbaeva, Eugene J. Thonar, Koichi Masuda, Albert C. Chen, Robert L. Sah, Andrew Davol, Stephen M. Klisch
Articular Cartilage Mechanical And Biochemical Property Relations Before And After In Vitro Growth, Timothy Ficklin, Gregory Thomas, James C. Barthel, Anna Asanbaeva, Eugene J. Thonar, Koichi Masuda, Albert C. Chen, Robert L. Sah, Andrew Davol, Stephen M. Klisch
Mechanical Engineering
The aim of this study was to design in vitro growth protocols that can comprehensively quantify articular cartilage structure–function relations via measurement of mechanical and biochemical properties. Newborn bovine patellofemoral groove articular cartilage explants were tested sequentially in confined compression (CC), unconfined compression (UCC), and torsional shear before (D0, i.e. day zero) and after (D14, i.e. day 14) unstimulated in vitro growth. The contents of collagen (COL), collagen-specific pyridinoline (PYR) crosslinks, glycosaminoglycan, and DNA significantly decreased during in vitro growth; consequently, a wide range of biochemical properties existed for investigating structure–function relations when pooling the D0 and D14 groups. All …