Civil Engineering Commons^™

Biodegradation Of The High Explosive Hexanitrohexaazaiso-Wurtzitane (Cl-20), Pelin Karakaya, Christos Christodoulatos, Agamemnon Koutsospyros, Wendy Balas, Steve Nicolich Nicolich, Mohammed Sidhoum

Civil Engineering Faculty Publications

The aerobic biodegradability of the high explosive CL-20 by activated sludge and the white rot fungus Phanerochaete chrysosporium has been investigated. Although activated sludge is not effective in degrading CL-20 directly, it can mineralize the alkaline hydrolysis products. Phanerochaete chrysosporium degrades CL-20 in the presence of supplementary carbon and nitrogen sources. Biodegradation studies were conducted using various nutrient media under diverse conditions. Variables included the CL-20 concentration; levels of carbon (as glycerol) and ammonium sulfate and yeast extract as sources of nitrogen. Cultures that received CL-20 at the time of inoculation transformed CL-20 completely under all nutrient conditions studied. When …