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An Investigation Into Bacterial Contamination In An Urban Nebraska Stream Using Microbial Source Tracking, Allison R. Speicher
An Investigation Into Bacterial Contamination In An Urban Nebraska Stream Using Microbial Source Tracking, Allison R. Speicher
Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research
High levels of fecal bacteria in surface waters are a public health concern due to the enteric illnesses caused by primary contact with these waters. Shortcomings in the current fecal indicator bacteria (FIB) monitoring approach and the ubiquity of pathogen-impaired water bodies has led to the development of microbial source tracking (MST), a molecular tool that can identify potential fecal contamination sources. In this study we use a bacterial community based approach examining the V4 region of the 16S rRNA gene to elucidate sources of fecal bacteria in Antelope Creek, an urban creek in Lincoln Nebraska.
We hypothesized that sediment …