Civil Engineering Commons^™

An Investigation Into Bacterial Contamination In An Urban Nebraska Stream Using Microbial Source Tracking, Allison R. Speicher

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

High levels of fecal bacteria in surface waters are a public health concern due to the enteric illnesses caused by primary contact with these waters. Shortcomings in the current fecal indicator bacteria (FIB) monitoring approach and the ubiquity of pathogen-impaired water bodies has led to the development of microbial source tracking (MST), a molecular tool that can identify potential fecal contamination sources. In this study we use a bacterial community based approach examining the V4 region of the 16S rRNA gene to elucidate sources of fecal bacteria in Antelope Creek, an urban creek in Lincoln Nebraska.

We hypothesized that sediment …

Development Of Best Management Practice Design Guidance For Roadway Applications In Nebraska, Benedict Vacha

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Runoff from roadways carries pollutants which may be detrimental to aquatic ecosystems. The primary pollutants of concern for roadway runoff are solids and heavy metals, particularly cadmium, copper, and zinc. Roadway runoff falls under the legislation of the Clean Water Act (CWA) via the National Pollutant Discharge Elimination System (NPDES). CWA regulates discharge of nonpoint source pollutants, such as roadway runoff, by issuing permits to public entities which manage Municipal Separate Storm Sewer Systems (MS4s). Part of the Nebraska Department of Roads (NDOR) permitting requirement is to create a design guide for Best Management Practices (BMPs) tailored to remediate roadway …