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Characterization Of Chitosan Films For Cell Culture Applications, Michael Katalinich

Electronic Theses and Dissertations

Chitosan (β-(l,4)-2-amino-2-deoxy-D-glucose) is a naturally occurring, abundant biopolymer exhibiting desirable biomaterial properties of biodegradability, low toxicity and good biocompatibility. These properties indicate the suitability of chitosan as a surface for mammalian cell growth. The goal of this thesis is to explore the potential for using chitosan as a surface for NIH 3T3 fibroblast attachment and growth. '4 standard, reproducible film-formation technique, based on other researchers techniques was established. The reproducibility of this technique. through characterization of the physical and chemical properties of these films, was good. The attachment and growth of NIH 3T3 fibroblasts on chitosan films and controls was …