Chemical Engineering Commons^™

Fluidization Of Nanoparticles, Caroline Hijung Nam

Dissertations

During the past decade, nanoparticles (1-100 nm) and nanocomposites have become the focus of many studies due to the unique properties of nanostructured materials that make them attractive for various applications. Due to their atomic and molecular interactions, nanoparticles and nanocomposites have unique and often favorable catalytic, mechanical, optical, electronic and / or other properties. For instance, nanocrystalline copper is up to 5 times harder than conventional micron sized copper particles. Nanocomposites, such as a homogenous mixture of different nanoparticles, can also exhibit improved properties. Other examples include coating and reacting nanoparticles with a second nanostructured phase. These processes are …

Novel Internally-Staged Ultrafiltration For Protein Purification, Meredith Ann Feins

Dissertations

A new ultrafiltration technique based on a multimembrane stack has been developed to fractionate proteins closer in molecular weight than conventionally possible. The technique is illustrated here by obtaining a pure protein product from a binary protein mixture. By employing membranes in series using the same membrane without any gaskets or spacers in-between, ultrafiltration is carried out to separate two proteins relatively close in molecular weight. Flat membranes, of the same molecular weight cutoff (MWCO) 30,000 or 100,000, are stacked together in the desired number, and ultrafiltration takes place. The membrane rejection of a protein is amplified with each additional …

Studies On Improved Integrated Membrane-Based Chromatographic Process For Bioseparation, Yanke Xu

Dissertations

To improve protein separation and purification directly from a fermentation broth, a novel membrane filtration-cum-chromatography device configuration having a relatively impermeable coated zone near the hollow fiber module outlet has been developed. The integrated membrane filtration-cum-chromatography unit packed with chromatographic beads on the shell side of the hollow fiber unit enjoys the advantages of both membrane filtration and chromatography; it allows one to load the chromatographic media directly from the fermentation broth or lysate and separate the adsorbed proteins through the subsequent elution step in a cyclic process.

Interfacial polymerization was carried out to coat the bottom section of the …