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Full-Text Articles in Biomedical Engineering and Bioengineering
Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies
Scanning Electron Microscopy Of The Osteoclast-Bone Interface In Vivo, H. Zhou, R. Chernecky, J. E. Davies
Cells and Materials
Rat femoral bones were studied by scanning electron microscopy to demonstrate the morphology of osteoclast- bone matrix interfacial relationships. Two general morphological types of actively resorbing osteoclasts were observed . One cell type was approximately ovoid with highly fimbriated borders, fully attached and closely adapted to the resorption surface. The ruffled border of such cells was composed of a number of individual filopodia which were of uniform , regular shape and approximately 2-3 μm in length and 150 nm in diameter. They were found to penetrate the bone matrix to a depth of 1 μm and interdigitated with the surrounding …
Primary Bone Formation In Porous Hydroxyapatite Ceramic: A Light And Scanning Electron Microscopic Study, M. Okumura, H. Ohgushi, S. Tamai, E. C. Shors
Primary Bone Formation In Porous Hydroxyapatite Ceramic: A Light And Scanning Electron Microscopic Study, M. Okumura, H. Ohgushi, S. Tamai, E. C. Shors
Cells and Materials
Porous hydroxyapatite ceramics combined with rat marrow cells were implanted subcutaneously in the back of syngeneic Fischer rats . Fluorochrome-labeling (calcein, tetracycline) was performed post-operatively and the ceramics were harvested 4 weeks after implantation. Undecalcified thin sections of the implants were observed under light microscopy or fluoromicroscopy and the corresponding areas were also analyzed in a scanning electron microscope connected to an electron probe microanalyzer (SEM-EPMA). Many pore areas of the ceramics showed bone and osteoid formation together with active osteoblasts. The bone formation began directly on the surface of the ceramic and proceeded in a centripetal direction towards the …