Biomedical Engineering and Bioengineering Commons^™

Modeling The Production Of Microalgal Biomass In Large Water Resource Recovery Facilities And Its Processing Into Various Commodity Bioproducts, James Pierson, Gopi Raju Makkena, Sandeep Kumar, Vinod Kumar, Vivekanand Vivekanand, Hasan Husain, Muhammad Ayser, Venkatesh Balan

Civil & Environmental Engineering Faculty Publications

Algae are capable of sequestering nutrients such as nitrates and phosphates from wastewater in the presence of sunlight and carbon dioxide (CO₂) to build up their body mass and help combat climate change. In the current study, we carried out different case studies to estimate the volume of algal biomass that could be produced annually using the rotating algal biofilm (RAB) method in three large-scale water resource recovery facilities (WRRFs) in Texas: Fort Worth, Dallas, and Houston. We calculated the total amount of lipids, carbohydrates, and proteins that could be fractionated from the algal biomass while using the …

Adaptive Laboratory Evolution Of Scenedesmus Obliquus For Increased Carbohydrate Content And Biomass Productivity, Nahel Ali

Master's Theses

The economics of microalgal bioproduct commercialization would benefit from increased accumulation of energy storage compounds, such as carbohydrates and lipids, and increased biomass productivity. This thesis explores two adaptive laboratory evolution strategies for improving Scenedesmus obliquus: single UV-mutagenesis and low light cultivation at a high dilution rate to produce cultigens with greater carbohydrate content and iterative UV-mutagenesis and selection under outdoor conditions in a raceway pond at a high dilution rate to increase biomass productivity.

Two cultigens were generated with the goal of increased carbohydrate content: K5 and K7. Both were mutagenized for 5 seconds and cultivated in 50-mL …

Methods To Increase Algae Biomass Productivity In Raceway Pond Monocultures, Ryan S. Anderson

Master's Theses

The economics of algae biofuels and bioproducts would be improved by increased biomass productivity. Two studies on this potential are described in this thesis – one on a locally isolated filamentous yellow-green alga and the other on a planktonic strain genetically improved via selective enrichment.

Polycultures have been viewed as productive, stable, and, in some cases, harvestable by natural bioflocculation. Local native strains might have higher productivity than culture collection strains because they are already adapted to local outdoor conditions. In this study, the filamentous yellow-green alga Tribonema minus was isolated from a local volunteer polyculture. Its productivity as a …

Preferentially Selecting Cellular Metabolism And Improving Productivity By Controlling Do And Pco2, Jiayi Zhang, Ryan Cassidy, Mark Emanuele, Gonzalo Milet, Lada Laenen

Cell Culture Engineering XV

Cells utilize glucose as their main resource for deriving energy through ATP production. The quantity of ATP generated depends on the metabolic pathways that are employed, aerobic glucose metabolism or anaerobic glucose metabolism. Using our bench top bioreactor model, we have shown these two metabolic pathways can be preferentially selected by controlling the desired cell culture DO and pCO2, and productivity was increased as a result. The DO and pCO2 controlling strategy was implemented in at-scale bioreactors and yielded the expected metabolic and productivity outcome

Bioreactor Process Improvements In A Legacy Perfusion-Based Process, Rakesh Motani, Gonzalo Milet, Gregory Walsh, Lada Laenen

Cell Culture Engineering XV

The legacy manufacturing processes for production of the enzyme products at Genzyme include long-term bioreactor perfusion-based cell culture platform. For the process with tight filed ranges and limited characterization, a phased approach is being used to improve bioreactor productivity. In the first phase, short-term process changes that are within filed and historical ranges were implemented. In the second phase, long-term process improvements that are outside the filed ranges will be implemented for a significant improvement in bioreactor productivity. Results from lab and at-scale study have confirmed that there was no adverse impact of phase 2 process improvements on cell culture, …

Rapid Development Of A Perfusion Process With High Productivity, Sen Xu, Linda Hoshan, Balrina Gupta, Hao Chen

Cell Culture Engineering XV

Continuous process improvement is required during a biologics process life cycle to increase process yield and reduce cost. In this poster, we will present a case study in late stage process development where everything was changed but the product quality. The following major changes were made when developing the new process:

• Production cell line – subclone of the original clone used.
• Media – from hydrolysate-containing feeds to fully chemically-defined feeds.
• Feeding strategy – from fixed volume/fixed schedule feeding to daily on-demand feeding.

Media components were optimized through spent media analysis and mechanistic understanding to accommodate the new feeding strategy. High-throughput …

From Fed-Batch To Perfusion: Productivity And Quality Considerations For A Late-Stage Program, Sen Xu, Hao Chen

Integrated Continuous Biomanufacturing II

In this poster, we will present the rapid development of a perfusion process with high productivity for late-stage manufacturing. The process uses a Chinese Hamster Ovary (CHO) cell line for monoclonal antibody production. Prior to the development, the clinical supply was manufactured by a fed-batch process with the same cell line.

To achieve desired productivity and comparable quality attributes, we developed a repeated batch shake flask model to rapidly screen media and process conditions. Shake flasks were inoculated at 10 × 10⁶ cells/mL and maintained in a CO₂ incubator with medium exchange for every 48 hours, repeated for …

Factors Affecting The Productivity Of 4-Column Periodic Counter Current Chromatography (4c-Pcc), Hani El-Sabbahy, Laura Fagan, Verity Nancollis

Integrated Continuous Biomanufacturing II

The advantages of continuous bioprocessing include steady state operation, reduced equipment footprint, high volumetric productivity, streamlined process flow, and reduced capital cost. These benefits have led to considerable interest in evaluating these technologies for the purposes of bioprocess intensification (Konstantinov & Cooney, 2014). It has been shown, through cost of goods modelling, that the cost of protein A is one of the most significant costs in a MAb process, and that decreasing the cost of protein A by maximising loading or increasing lifetime will have a significant effect on consumable costs (Broly, et al., 2010). Consequently, the application of continuous …

Strategy For Scaling Semi-Continuous Downstream And Integration Of Process Analytical Tools For Monoclonal Antibody Toxicology, Darshini Shah, Rebecca Chmielowski, Colette Cutler, Hong Li, David Roush, Nihal Tugcu

Integrated Continuous Biomanufacturing II

Currently, continuous processing for biologics is being pursued from a technology perspective in order to increase productivity and decrease cost of manufacture. Disruptive technologies, such as PCC (Periodic Counter-current Chromatography), have been researched extensively in order to reduce the bottleneck for the downstream capture chromatography step. However, there is extremely limited knowledge in how to scale these disruptive technologies for clinical and commercial manufacturing. In this presentation, we demonstrate a strategy for scaling and integration of a PCC capture step into a semi-continuous downstream process (Protein A chromatography-Viral inactivation-Filtration-Anion exchange chromatography). Harvest cell culture fluid (HCCF) feed streams ranging from …

Effect Of Glucose Supplementation On Nighttime Biomass Loss And Productivity Of Microalgae Chlorella, Dhruvesh Shah

ETD Archive

Microalgae have been proven to be a promising source for the production of biofuel. It has higher oil yield than any other food crop. Oil yield from microalgae is 7 to 13 times higher than the best food crop. There are several issues to be addressed for economic production of biofuel from microalgae. One such issue is cultivation of microalgae. Algae can be cultivated photoautotrophically or heterotrophically. Algae grown photoautotrophically lose up to 35 of the biomass during nighttime. Heterotrophic cultivation of microalgae has been proven to be resulting in faster microalgae production compared to photoautotrophic growth, but requires use …