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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
Pixel-Based Absorption Correction For Dual-Tracer Fluorescence Imaging Of Receptor Binding Potential, Stephen C. Kanick, Kenneth M. Tichauer, Jason Gunn, Kimberley S. Samkoe, Brian W. Pogue
Pixel-Based Absorption Correction For Dual-Tracer Fluorescence Imaging Of Receptor Binding Potential, Stephen C. Kanick, Kenneth M. Tichauer, Jason Gunn, Kimberley S. Samkoe, Brian W. Pogue
Dartmouth Scholarship
Ratiometric approaches to quantifying molecular concentrations have been used for decades in microscopy, but have rarely been exploited in vivo until recently. One dual-tracer approach can utilize an untargeted reference tracer to account for non-specific uptake of a receptor-targeted tracer, and ultimately estimate receptor binding potential quantitatively. However, interpretation of the relative dynamic distribution kinetics is confounded by differences in local tissue absorption at the wavelengths used for each tracer. This study simulated the influence of absorption on fluorescence emission intensity and depth sensitivity at typical near-infrared fluorophore wavelength bands near 700 and 800 nm in mouse skin in order …
Sub-Diffusive Scattering Parameter Maps Recovered Using Wide-Field High-Frequency Structured Light Imaging, Stephen C. Kanick, David M. Mcclatchy, Venkataramanan Krishnaswamy, Jonathan T. Elliott, Keith D. Paulsen, Brian W. Pogue
Sub-Diffusive Scattering Parameter Maps Recovered Using Wide-Field High-Frequency Structured Light Imaging, Stephen C. Kanick, David M. Mcclatchy, Venkataramanan Krishnaswamy, Jonathan T. Elliott, Keith D. Paulsen, Brian W. Pogue
Dartmouth Scholarship
This study investigates the hypothesis that structured light reflectance imaging with high spatial frequency patterns (fx) can be used to quantitatively map the anisotropic scattering phase function distribution (P(θs)) in turbid media. Monte Carlo simulations were used in part to establish a semi-empirical model of demodulated reflectance (Rd) in terms of dimensionless scattering (μ′sf−1x) and γ, a metric of the first two moments of the P(θs) distribution. Experiments completed in tissue-simulating phantoms showed that simultaneous analysis of Rd spectra sampled at multiple f …
A Gamos Plug-In For Geant4 Based Monte Carlo Simulation Of Radiation-Induced Light Transport In Biological Media, Adam K. Glaser, Stephen C. Kanick, Rongxiao Zhang, Pedro Arce, Brian W. Pogue
A Gamos Plug-In For Geant4 Based Monte Carlo Simulation Of Radiation-Induced Light Transport In Biological Media, Adam K. Glaser, Stephen C. Kanick, Rongxiao Zhang, Pedro Arce, Brian W. Pogue
Dartmouth Scholarship
We describe a tissue optics plug-in that interfaces with the GEANT4/GAMOS Monte Carlo (MC) architecture, providing a means of simulating radiation-induced light transport in biological media for the first time. Specifically, we focus on the simulation of light transport due to the Čerenkov effect (light emission from charged particle's traveling faster than the local speed of light in a given medium), a phenomenon which requires accurate modeling of both the high energy particle and subsequent optical photon transport, a dynamic coupled process that is not well-described by any current MC framework. The results of validation simulations show excellent agreement with …
Singular Value Decomposition Metrics Show Limitations Of Detector Design In Diffuse Fluorescence Tomography, Frederic Leblond, Kenneth M. Tichauer, Brian W. Pogue
Singular Value Decomposition Metrics Show Limitations Of Detector Design In Diffuse Fluorescence Tomography, Frederic Leblond, Kenneth M. Tichauer, Brian W. Pogue
Dartmouth Scholarship
The spatial resolution and recovered contrast of images reconstructed from diffuse fluorescence tomography data are limited by the high scattering properties of light propagation in biological tissue. As a result, the image reconstruction process can be exceedingly vulnerable to inaccurate prior knowledge of tissue optical properties and stochastic noise. In light of these limitations, the optimal source-detector geometry for a fluorescence tomography system is non-trivial, requiring analytical methods to guide design. Analysis of the singular value decomposition of the matrix to be inverted for image reconstruction is one potential approach, providing key quantitative metrics, such as singular image mode spatial …
Critical Computational Aspects Of Near Infrared Circular Tomographic Imaging: Analysis Of Measurement Number, Mesh Resolution And Reconstruction Basis, Phaneendra K. Yalavarthy, Hamid Dehghani, Brian W. Pogue, Keit D. Paulsen
Critical Computational Aspects Of Near Infrared Circular Tomographic Imaging: Analysis Of Measurement Number, Mesh Resolution And Reconstruction Basis, Phaneendra K. Yalavarthy, Hamid Dehghani, Brian W. Pogue, Keit D. Paulsen
Dartmouth Scholarship
The image resolution and contrast in Near-Infrared (NIR) tomographic image reconstruction are affected by parameters such as the number of boundary measurements, the mesh resolution in the forward calculation and the reconstruction basis. Increasing the number of measurements tends to make the sensitivity of the domain more uniform reducing the hypersensitivity at the boundary. Using singular-value decomposition (SVD) and reconstructed images, it is shown that the numbers of 16 or 24 fibers are sufficient for imaging the 2D circular domain for the case of 1% noise in the data. The number of useful singular values increases as the logarithm of …